Activities in Sub Family	Endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (10); Uniprot accession (37); PDB accession (10); 3D entries (6); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
C6W10_17835		Plantactinospora sp. BB1	AVT37994.1			8
C6361_28680		Plantactinospora sp. BC1	AVT32781.1			8
C6361_04175		Plantactinospora sp. BC1	AVT28826.1			8
CIK06_09835		Plantactinospora sp. KBS50	ASW54425.1			8
Prubr_59870		Polymorphospora rubra NBRC 101157	BCJ68966.1			8
Prubr_45610		Polymorphospora rubra NBRC 101157	BCJ67540.1			8
C9I28_17010		Pseudoduganella armeniaca ZMN-3	AVR97152.1			8
β-1,4-mannanase (AmnA)	3.2.1.78	Pseudomonas sp. ND137	BAB79290.2	Q8RR71		8
Rumal_1363		Ruminococcus albus 7 = DSM 20455	ADU21877.1			8
Rumal_2599		Ruminococcus albus 7 = DSM 20455	ADU23074.1			8
RBI_I00637 (ManA)		Ruminococcus bicirculans (ex Wegman et al. 2014) 80/3	CCO04360.1			8
β-mannanase (GH5C;RUM_21650) (Man5A)	3.2.1.78	Ruminococcus champanellensis 18P13 = JCM 17042	CBL18180.1	D4LEY5		8
glucomannanase (RfGH5-1/2)		Ruminococcus flavefaciens	WP_009984467.1			8
C2869_05895		Saccharobesus litoralis CCB-QB4	AWB65998.1			8
Sde_0064 (Man5N)		Saccharophagus degradans 2-40	ABD79328.1			8
β-mannanase (Sde_0656) (Man5O)	3.2.1.78	Saccharophagus degradans 2-40	ABD79918.1	Q21N11		8
QFX18_18065		Saccharophagus degradans FZY0027	WGO97918.1			8
QFX18_00245		Saccharophagus degradans FZY0027	WGP00529.1			8
GCM10017745_70320		Saccharothrix mutabilis subsp. capreolus JCM 4248	BFE53605.1			8
IOD16_09275		Saccharothrix sp. 6-C	QQQ78619.1			8
IOD16_12655		Saccharothrix sp. 6-C	QQQ79201.1			8
IOD16_13025		Saccharothrix sp. 6-C	QQQ79259.1			8
L3Q67_29510		Saccharothrix sp. AJ9571	UJW29359.1			8
EKG83_20685		Saccharothrix syringae NRRL B-16468	QFZ19526.1			8
EKG83_12065		Saccharothrix syringae NRRL B-16468	QFZ18119.1			8
EKG83_22410		Saccharothrix syringae NRRL B-16468	QFZ19812.1			8
β-mannanase 5A (BaMan5A) (Man5A)	3.2.1.78	Salipaludibacillus agaradhaerens	AAN27517.1 AAN27518.1 AAQ31837.1 AAQ79152.1 AAQ79153.1 ABC13790.1 ABC13791.1	G1K3N4 Q5YEX6	2WHJ[A] 2WHL[A]	8
MM221_05580		Salipaludibacillus sp. LMS25	UTR16031.1			8
G7063_10220		Sanguibacter sp. HDW7	QIK83955.1			8
DUT88_01665		Shouchella clausii 088AE	QNM45522.1			8
Q7C08_04400		Shouchella clausii 2102	WMM33363.1			8
SR921_01525		Shouchella clausii ATCC 700160	WQG95481.1			8
BC8716_04830		Shouchella clausii DSM 8716	AST98531.1			8
DB29_01746		Shouchella clausii ENTPro	ALA52574.1			8
ABC0320		Shouchella clausii KSM-K16	BAD62862.1			8
N7M99_04120		Shouchella clausii SF174	UXN55621.1			8
V5G21_10920		Shouchella rhizosphaerae CP20	WWA28283.1			8
O7608_24935		Solwaraspora sp. WMMA2056	WJK39668.1			8
O7553_21645 (fragment)		Solwaraspora sp. WMMA2059	WBB95937.1			8
O7553_08115		Solwaraspora sp. WMMA2059	WBB98838.1			8
O7610_02865		Solwaraspora sp. WMMA2065	WJK35341.1			8
O7610_15835 (fragment)		Solwaraspora sp. WMMA2065	WJK32255.1			8
O7543_25730 (fragment)		Solwaraspora sp. WMMA2080	WBC20159.1			8
O7543_09250		Solwaraspora sp. WMMA2080	WBC22609.1			8
O7623_00755		Solwaraspora sp. WMMD791	WFE27780.1			8
O7621_10865		Solwaraspora sp. WMMD937	WFE23721.1			8
O7621_16825		Solwaraspora sp. WMMD937	WFE19593.1			8
STHERM_c12480		Spirochaeta thermophila DSM 6192	ADN02189.1			8
Spith_1286		Spirochaeta thermophila DSM 6578	AEJ61551.1			8
GO596_01805		Streptococcus equinus CNU 77-23	QGX44134.1			8
GPA00_04115		Streptococcus equinus CNU G6	QGX46302.1			8
H1R75_01435		Streptococcus equinus MDC1	QMS96580.1			8
NCTC10389_00101 (Gmug_2) (fragment)		Streptococcus equinus NCTC10389	VEE21077.1			8
NCTC10389_00100 (Gmug_1) (fragment)		Streptococcus equinus NCTC10389	VEE21075.1			8
NCTC11436_00058 (GmuG) (fragment)		Streptococcus equinus NCTC11436	VEB79170.1			8
NCTC8140_00242 (GmuG)		Streptococcus equinus NCTC8140	VED90612.1			8
KRG72_01225		Streptococcus equinus S1	UVF02955.1			8
KIP81_01305		Streptococcus equinus SheepZ001	UOC11361.1			8
FOC63_04970		Streptococcus gallolyticus FDAARGOS_755	QKI00895.1			8
NCTC13773_00214 (GmuG)		Streptococcus gallolyticus NCTC13773	SQG78449.1			8
SGGB_0206 (GmuG)		Streptococcus gallolyticus subsp. gallolyticus ATCC 43143	BAK27103.1			8
SGGBAA2069_c01800		Streptococcus gallolyticus subsp. gallolyticus ATCC BAA-2069	CBZ47352.1			8
BTR42_00825		Streptococcus gallolyticus subsp. gallolyticus DSM 16831	AQP41170.1			8
NCTC8133_00229 (GmuG)		Streptococcus gallolyticus subsp. gallolyticus NCTC8133	VTS81291.1			8
JGX27_01060		Streptococcus gallolyticus subsp. gallolyticus TX20005	QWX86966.1			8
GALLO_0162		Streptococcus gallolyticus UCN34	CBI12654.1			8
OIY87_02430		Streptococcus gallolyticus XH2168	WAW99227.1			8
GO995_01385		Streptococcus infantarius CNU G3	QGW99981.1			8
AU079_01000 (fragment)		Streptococcus infantarius ICDDRB-NRC-S5	ALT81993.1			8
NCTC13774_00217 (GmuG) (fragment)		Streptococcus lutetiensis NCTC13774	SQG55543.1			8
NCTC8738_00214 (GmuG) (fragment)		Streptococcus lutetiensis NCTC8738	SQF41473.1			8
NCTC8796_01659 (GmuG) (fragment)		Streptococcus lutetiensis NCTC8796	VTT08225.1			8
GPZ88_05395		Streptococcus ruminicola CNU G2	QIM46502.1			8
P7Y79_08225		Streptococcus ruminicola CNU_77-47	WFM81543.1			8
GP482_09170		Streptococcus sp. CNU 77-61	QGZ28267.1			8
EKD16_00235 (Mana1)		Streptomonospora litoralis M2	QBI51874.1			8
OUQ99_16310		Streptomonospora nanhaiensis 12A09	WAE70807.1			8
DMT42_29510		Streptomyces actuosus ATCC 25421	AWT46021.1			8
K1J60_40575		Streptomyces akebiae MG28	QYX82013.1			8
K1J60_38525		Streptomyces akebiae MG28	QYX83409.1			8
H9W91_34690		Streptomyces alfalfae XN-04	QUI35402.1			8
SAM23877_4871 (ManA)		Streptomyces ambofaciens ATCC 23877	AKZ57916.1			8
SAM23877_6969		Streptomyces ambofaciens ATCC 23877	AKZ60014.1			8
SAMR0614		Streptomyces ambofaciens ATCC 23877	CAJ88324.1			8
SAM40697_4389		Streptomyces ambofaciens DSM 40697	ANB08347.1			8
SAM40697_6286		Streptomyces ambofaciens DSM 40697	ANB10239.1			8
G9272_05275		Streptomyces asoensis H2S5	QJT06651.1			8
CD934_30235		Streptomyces asterosporus DSM 41452	QDI72496.1			8
O1Q96_29225		Streptomyces aurantiacus Qhu-M197	WAU83413.1			8
O1Q96_27190		Streptomyces aurantiacus Qhu-M197	WAU83049.1			8
BV401_06615		Streptomyces autolyticus CGMCC0516	AQA16021.1			8
SBI_09150		Streptomyces bingchenggensis BCW-1	ADI12268.1			8
EJC51_05095		Streptomyces brunneus CR22	AZP15527.1			8
IHE65_38655		Streptomyces caniscabiei ID-03-3A	WEO28625.1			8
IHE65_40820		Streptomyces caniscabiei ID-03-3A	WEO29003.1			8
OG925_41530		Streptomyces canus NBC_01585	WSD90407.1			8
N0X72_25785		Streptomyces carpaticus SCPM-O-B-9993	UWM52155.1			8
PYS65_31605		Streptomyces sp. HUAS 5	WGD44318.1			8
NRO40_23045		Streptomyces changanensis HL-66	UUS33401.1			8
CP983_39955		Streptomyces chartreusis ATCC 14922	QEV72209.1			8
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