Activities in Sub Family	Endo-β-1,4-mannanase (EC 3.2.1.78);
Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (10); Uniprot accession (37); PDB accession (10); 3D entries (6); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
Calkro_0851		Caldicellulosiruptor kronotskyensis 2002	ADQ45728.1			8
Calkro_0854		Caldicellulosiruptor kronotskyensis 2002	ADQ45730.1			8
Calkro_0856 (fragment)		Caldicellulosiruptor kronotskyensis 2002	ADQ45732.1			8
OTK00_002183		Caldicellulosiruptor morganii DSM 8990	WAM33661.1			8
OTJ99_000852		Caldicellulosiruptor naganoensis DSM 8991	WAM32321.1			8
OTJ99_000849		Caldicellulosiruptor naganoensis DSM 8991	WAM32678.1			8
OTJ99_000858		Caldicellulosiruptor naganoensis DSM 8991	WAM32679.1			8
COB47_1669		Caldicellulosiruptor obsidiansis OB47	ADL42950.1			8
bifunctional β-mannanase / endo-β-1,4-glucanase (ManA)	3.2.1.78	Caldicellulosiruptor saccharolyticus	AAA71887.1 AAA72861.1	P22533		8
Csac_1077 (ManA)		Caldicellulosiruptor saccharolyticus DSM 8903	ABP66691.1			8
Csac_1080 (ManB) (fragment)		Caldicellulosiruptor saccharolyticus DSM 8903	ABP66694.1			8
CS0771_62390		Catellatospora sp. IY07-71	BCJ76695.1			8
CS0771_27670		Catellatospora sp. IY07-71	BCJ73223.1			8
C1707_15825		Caulobacter flavus RHGG3	AYV49609.1			8
CA608_04095		Caulobacter vibrioides CB2	ATC23771.1			8
NP064_14475		Cellulomonas chengniuliangii zg-Y338	UUI74970.1			8
KKR89_13905		Cellulomonas dongxiuzhuiae zg-ZUI157	QWC15390.1			8
KG102_14195		Cellulomonas fengjieae zg-ZUI188	QVI65258.1			8
Cfla_2811		Cellulomonas flavigena DSM 20109	ADG75696.1			8
Celgi_2637		Cellulomonas gilvus ATCC 13127	AEI13136.1			8
LFM56_04035		Cellulomonas iranensis ZJW-6	UCN15505.1			8
F1D97_17090		Cellulomonas palmilytica EW123	UJP39960.1			8
E5225_03685		Cellulomonas shaoxiangyii Z28	QCB92788.1			8
GXP71_12270		Cellulomonas sp. H30R-01	QHT56776.1			8
GC089_03930		Cellulomonas sp. JZ18	QGQ18559.1			8
CBP52_10310		Cellulomonas sp. PSBB021	ASR55418.1			8
OKX07_15650		Cellulomonas sp. S1-8	UZN02476.1			8
KG103_14245		Cellulomonas wangleii zg-ZUI222	QVI61610.1			8
NP075_14595		Cellulomonas wangsupingiae zg-Y908	UUI64340.1			8
NP048_14230		Cellulomonas xiejunii zg-B89	UUI70941.1			8
H1Q78_09060		Cellulosimicrobium cellulans 2020WEIHUA_E	UKJ65424.1			8
H1Q78_09065		Cellulosimicrobium cellulans 2020WEIHUA_E	UKJ65425.1			8
CBR64_11685		Cellulosimicrobium cellulans PSBB019	ARU52036.1			8
CBR64_01715		Cellulosimicrobium cellulans PSBB019	ARU50408.1			8
CBR64_01710		Cellulosimicrobium cellulans PSBB019	ARU50407.1			8
NMQ07_13550		Cellulosimicrobium cellulans TL2	UTT58277.1			8
NMQ07_13555		Cellulosimicrobium cellulans TL2	UTT58278.1			8
endo-β-1,4-mannanase (ManB)		Cellulosimicrobium cellulans YB-43	AQQ16388.1			8
β-1,4-mannanase (ManH)	3.2.1.78	Cellulosimicrobium sp. HY-13	AEE43708.1	G3FBL1		8
FY115_16115		Cellvibrio japonicus ADPT1-KOJIBIOSE	QEI20734.1			8
FY115_11945		Cellvibrio japonicus ADPT1-KOJIBIOSE	QEI21361.1			8
FY116_11950		Cellvibrio japonicus ADPT2-NIGEROSE	QEI17786.1			8
FY116_16120		Cellvibrio japonicus ADPT2-NIGEROSE	QEI17157.1			8
FY117_11945		Cellvibrio japonicus ADPT3-ISOMALTOSE	QEI14209.1			8
FY117_16115		Cellvibrio japonicus ADPT3-ISOMALTOSE	QEI13583.1			8
endo-β-1,4-mannanase 5A (CjMan5A; CJA_3338) (Man5A)	3.2.1.78	Cellvibrio japonicus Ueda107	ACE84673.1 AAO31759.1	B3PF24 Q840C2		8
endo-β-1,4-mannanase 5B (CJA_2475) (Man5B)	3.2.1.78	Cellvibrio japonicus Ueda107	AAO31760.1 ACE84941.1	B3PKK7 Q840C1		8
D0C16_14850		Cellvibrio sp. KY-GH-1	QEY19054.1			8
D0B88_02480		Cellvibrio sp. KY-YJ-3	QEY11219.1			8
D0B88_05675		Cellvibrio sp. KY-YJ-3	QEY11805.1			8
CBR65_07645		Cellvibrio sp. PSBB006	ARU29988.1			8
NNX04_12935		Cellvibrio sp. QJXJ	UUA71317.1			8
NNX04_16415		Cellvibrio sp. QJXJ	UUA71990.1			8
glycosyl hydrolase family 5_8 domain-containing protein, partial		Cellvibrionaceae bacterium Bs08	AIF91546.1			8
glycosyl hydrolase family 5_8 domain-containing protein		Cellvibrionaceae bacterium Bs12	AIF91534.1			8
CM240_1811		Clostridium bornimense M2/40	CDM68969.1			8
HLV60_02965		Clostridium butyricum 16-3	QJU43361.1			8
FBD77_05345		Clostridium butyricum 29-1	QCJ02105.1			8
FBD76_03930		Clostridium butyricum 4-1	QCJ05579.1			8
Q9978_05970		Clostridium butyricum CBM588	WLS69050.1			8
KDJ93_12955		Clostridium butyricum CBUT	QUF82632.1			8
NPD4_1214		Clostridium butyricum CDC_51208	APF25044.1			8
HNS01_14185		Clostridium butyricum CFSA-TJ-E	UTY54187.1			8
EBL75_17275		Clostridium butyricum CFSA3987	QGH23260.1			8
EBQ27_17275		Clostridium butyricum CFSA3989	QGH27303.1			8
QVH08_14925		Clostridium butyricum DKU-11	WJV33520.1			8
JZY05_13875		Clostridium butyricum DS501	QSX02130.1			8
FF104_04180		Clostridium butyricum DSM 10702	QMW90174.1			8
ATD26_04905		Clostridium butyricum JKY6D1	ALS16221.1			8
ATN24_06355		Clostridium butyricum KNU-L09	ALP89769.1			8
Cbu04g_27490		Clostridium butyricum NBRC 13949	BBK77741.1			8
DRB99_03605		Clostridium butyricum S-45-5	AXB84071.1			8
BBB49_04985		Clostridium butyricum TK520	AOR93452.1			8
AZ909_04800		Clostridium butyricum TOA	ANF13383.1			8
O4N14_13955		Clostridium butyricum WQ1	WBP98363.1			8
C1I91_04495		Clostridium manihotivorum CT4	QAA30979.1			8
CSACC_27090 (Mana_2)		Clostridium saccharobutylicum BAS/B3/SW/136	AQS00885.1			8
CLSA_c27120		Clostridium saccharobutylicum DSM 13864	AGX43683.1			8
CLOSACC_27090 (Mana_2)		Clostridium saccharobutylicum NCP 195	AQS14868.1			8
CLOSC_27020 (Mana_2)		Clostridium saccharobutylicum NCP 200	AQR90981.1			8
CLOBY_27680 (Mana_2)		Clostridium saccharobutylicum NCP 258	AQS10623.1			8
ONV75_05090		Clostridium sp. LQ25	UZT07236.1			8
FPL14_19700		Cohnella cholangitidis 1605-214	QMV43160.1			8
COUCH_24875		Couchioplanes caeruleus TL65	UQU62260.1			8
N8J89_16995		Crossiella sp. CA-258035	WHT22694.1			8
F0U63_15495		Cystobacter fuscus Cbf 10	WNG15906.1			8
F0U62_16535		Cystobacter fuscus Cbf 8	WNG25439.1			8
CYFUS_003189		Cystobacter fuscus DSM 52655	ATB37764.1			8
RCC89_12495		Cytophagaceae bacterium ABcell3	WMJ73976.1			8
Daura_43730		Dactylosporangium aurantiacum NRRL B-8018	UWZ53387.1			8
Daura_17555		Dactylosporangium aurantiacum NRRL B-8018	UWZ57812.1			8
Daura_43775		Dactylosporangium aurantiacum NRRL B-8018	UWZ53394.1			8
Daura_28235		Dactylosporangium aurantiacum NRRL B-8018	UWZ59799.1			8
Daura_12145		Dactylosporangium aurantiacum NRRL B-8018	UWZ56856.1			8
Dfulv_32760		Dactylosporangium fulvum NRRL B-16292	UWP79917.1			8
Dfulv_40190		Dactylosporangium fulvum NRRL B-16292	UWP81283.1			8
Dfulv_40005		Dactylosporangium fulvum NRRL B-16292	UWP81247.1			8
Dmats_39810		Dactylosporangium matsuzakiense NRRL B-16293	UWZ43541.1			8
Dmats_12675		Dactylosporangium matsuzakiense NRRL B-16293	UWZ47182.1			8
Dmats_39785		Dactylosporangium matsuzakiense NRRL B-16293	UWZ43536.1			8
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