Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54780); Uniprot accession (7858); PDB accession (467); 3D entries (111); cryst (2)
All (50004) Archaea (367) Bacteria (46504) Eukaryota (3003) unclassified (130) Structure (111 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Archaea
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 pullulanase (ApuA;Pul-Dm;Desmu_0653) 3.2.1.41 Desulfurococcus mucosus DSM 2162 AAG31033.1
ADV64961.1
E8R8Y5
Q9HHB0
  20
 α-amylase (AmyH) 3.2.1.1 Haloarcula hispanica CAI64586.1 Q4A3E0  
 α-amylase (MalA) 3.2.1.1 Haloarcula japonica TR-1 BAM75337.1    
 α-cyclomaltodextrin glucanotransferase (Cgt) 2.4.1.19 Haloferax mediterranei CAI46245.1 Q53I75   2
 maltooligosyltrehalose trehalohydrolase (TreZ;MTH) 3.2.1.141 Metallosphaera hakonensis JCM 8857 AAR18372.1 Q6SZP6   10
 maltooligosyltrehalose synthase (TreY;MTS) 5.4.99.15 Metallosphaera hakonensis JCM 8857 AAR18371.1 Q6SZP7   26
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116
Natronococcus sp. Ah-36 BAA05516.1 Q60224  
 trehalose synthase (PTO0069) 5.4.99.16 Picrophilus torridus DSM 9790 AAT42654.1
CBF79071.1
Q6L2Z7   16
 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13) 3.2.1.1
3.2.1.54
Pyrococcus furiosus DSM 3638 AAL82063.1
CAZ78721.1
NP_579668.1
Q8TZP8 4AEF[A,B] 20
 β-cyclodextrin glucanotransferase (PfCgt;PF0478;PF0478*) 2.4.1.19 Pyrococcus furiosus DSM 3638 ABA33720.1
AAL80602.1
ABA30024.1
ABA30025.1
NP_578207.1
Q3HUR2
Q8U3I8
  2
 α-amylase (AmyA;PF0477) 3.2.1.1 Pyrococcus furiosus DSM 3638 AAL80601.1
AAB67705.1
AAC45663.1
NP_578206.1
O08452
Q8U3I9
  7
 α-amylase (AmyA) 3.2.1.1 Pyrococcus woesei AAD54338.1
AAF44693.1
ACH26211.1
Q7LYT7
Q9P9L0
1MWO[A]
1MXD[A]
1MXG[A]
7
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) 3.2.1.133
3.2.1.54
Staphylothermus marinus F1 ABN69720.1 A3DM60 4AEE[A,B] 20
 α-amylase/maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.1
3.2.1.141
Sulfolobus acidocaldarius BAA11011.1
BAA11863.1
Q53641   10
 maltooligosyltrehalose synthase (TreY;MTSase) 5.4.99.15 Sulfolobus acidocaldarius BAA11009.1
BAA11865.1
Q53688 1IV8[A] 26
 isoamylase (TreX) 3.2.1.68 Sulfolobus acidocaldarius ATCC33909 BAA11864.1 O05152   11
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Sulfolobus shibatae AAF17554.1
AAM81591.1
Q9UWN8   26
 maltooligosyl trehalose trehalohydrolase (TreZ) 3.2.1.141 Sulfolobus shibatae AAF17553.1
AAM81589.1
Q9UWN9   10
 glycogen debranching enzyme (TreX) 2.4.1.25
3.2.1.68
Sulfolobus shibatae B12 AAM81590.1 Q8NKQ3   11
 α-amylase / maltooligosyltrehalose trehalohydrolase (GTHase) 3.2.1.1
3.2.1.141
Sulfolobus solfataricus KM1 AAR46454.1
BAA11010.1
Q55088 1EH9[A]
1EHA[A]
3VGB[A]
3VGD[A]
3VGE[A]
3VGF[A]
3VGG[A]
3VGH[A]
10
 glycosyltrehalose-producing enzyme 5.4.99.15 Sulfolobus solfataricus KM1 BAA11008.1 Q55262   26
 maltooligosyltrehalose synthase (TreY;SSO2095) 5.4.99.15 Sulfolobus solfataricus P2 AAK42274.1
CAA69505.1
NP_343484.1
P95869
Q7LX98
  26
 maltooligosyltrehalose trehalohydrolase (TreZ;MTHase;SSO2093;c06020) 3.2.1.141 Sulfolobus solfataricus P2 AAK42272.1
CAA69503.1
CAC23737.1
NP_343482.1
P95867   10
 isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25
3.2.1.68
Sulfolobus solfataricus P2 AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868
Q7LX99
2VNC[A,B]
2VR5[A,B]
2VUY[A,B]
11
 pullulanase type III (PulhA;Pul-Ta) 3.2.1.41 Thermococcus aggregans CAB94218.1
CAZ78888.1
Q9P9A0   20
 cyclomaltodextrinase (CD;TcCD;CL1_0884) 3.2.1.54 Thermococcus cleftensis AFL95089.1     20
 α-amylase (Amy) 3.2.1.1 Thermococcus hydrothermalis AL662 AAC97877.1
AAC97878.1
ACH26213.1
O93647
O93648
  7
 β-cyclodextrin glucanotransferase (Cgt;TK-cgt;TK2172) 2.4.1.19 Thermococcus kodakarensis KOD1 BAB78538.1
BAD86361.1
CAT70763.1
Q8X268   2
 cyclodextrinase (CDase-Tk;TK1770) 3.2.1.54 Thermococcus kodakarensis KOD1 BAD85959.1
CAT70564.1
Q5JJ59   20
 amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977) 3.2.1.1
3.2.1.41
Thermococcus kodakarensis KOD1 BAD85166.1
CAT69621.1
CAZ78793.1
Q5JID9   20
 α-amylase (ApkA;TK1884) 3.2.1.1 Thermococcus kodakarensis KOD1 BAA21130.1
ACH26210.1
BAD86073.1
CAT70635.1
O33476
Q5JER7
  7
 cyclomaltodextrinase (Tocd;TON_1796) 3.2.1.54 Thermococcus onnurineus NA1 ACJ17286.1 B6YV58   20
 α-amylase (TO_Amyl;TON_1660) 3.2.1.1 Thermococcus onnurineus NA1 ABA26948.1
ACJ17150.1
B6YUG5   7
 α-amylase (BD5031) 3.2.1.1 Thermococcus sp. AEPII 1a AAM48113.1
ACH26099.1
Q8NKR4   7
 α-cyclodextrin glucanotransferase (CgtA) 2.4.1.19 Thermococcus sp. B1001 BAA88217.1
BAB18101.1
CAZ78889.1
Q9UWN2   2
 cyclomaltodextrinase (CdnA) 3.2.1.133
3.2.1.54
Thermococcus sp. B1001 BAB18100.1
CAZ78887.1
Q9HHC8   20
 α-amylase (BD5064) 3.2.1.1 Thermococcus sp. GU5L5 AAM48112.1
ACH26098.1
Q8NKR5   7
 α-amylase (THJA) 3.2.1.1 Thermococcus sp. HJ21 ABU98335.1 B4X9V8   7
 α-amylase 3.2.1.1 Thermococcus sp. Rt3 AAB87860.1
ACH26212.1
O50200   7
 α-amylase (Amy) 3.2.1.1 Thermococcus thioreducens AAT11125.1 Q66SB4   7
 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458) 3.2.1.133
3.2.1.54
Thermofilum pendens Hrk 5 ABL78855.1 A1S075   20
 maltogenic α-amylase (TpMA;TVG0226058) 3.2.1.133 Thermoplasma volcanium GSS1 BAB59359.1
CAZ78693.1
NP_110736.1
Q97C86   20
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 acarbose resistent α-amylase (AcbE;ACPL_3683) 3.2.1.1 Actinoplanes sp. SE50/110 AEV84578.1
CAC02970.1
CAJ81030.1
SLM00378.1
Q27GR6
Q9K5L6
 
 acarviosyltransferase (AcbD; ACPL_3684) 2.4.1.- Actinoplanes sp. SE50/110 CAC02971.1
AAE37556.1
AEV84579.1
CAJ81031.1
SLM00379.1
Q9K5L5   2
 trehalose synthase (TreS) 5.4.99.16 Actinoplanes sp. SN223/29 ABV26719.1 A8QX00   16
 malto-oligosyltrehalose synthase (TreY) 5.4.99.15 Actinoplanes sp. SN223/29 ABV26725.1 A8QX07   26
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141 Actinoplanes sp. SN223/29 ABV26726.1 A8QX08   10
 α-amylase 3.2.1.1 Aeromonas hydrophila JMP636 AAA21936.1 P22630   27
 α-amylase (AmyA) 3.2.1.1 Aeromonas hydrophila MCC-1 AAA21016.1 P41131   32
 branching-enzyme (GlgB) 2.4.1.18 Agrobacterium tumefaciens A348 AAD03472.1 P52979   9
 sucrose phosphorylase 2.4.1.7 Agrobacterium vitis CAA80424.1
CAA80426.1
CAA80428.1
P33910   18
 amylase-pullulanase (Amy;Aaci_2874) 3.2.1.1
3.2.1.41
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59878.1
CAA44638.1
CAZ78868.1
C8WUR2
Q06307
  39
 cyclomaltodextrinase (CdaA;Aaci_2869) 3.2.1.54 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59873.1
CAB40078.1
CAB65655.1
CAY02967.1
CAZ78890.1
C8WUQ7
Q9WX32
  20
 α-amylase (AmyA4) 3.2.1.1 Alicyclobacillus sp. A4 / CGMCC 3147 ADZ99363.1     39
 α-amylase (Amy1;AmyA;AmyB) 3.2.1.1 Alkalimonas amylolytica N10 AAQ01675.1 Q6WUB6   5
 α-amylase (AmySTU) 3.2.1.1 Alteromonas macleodii B7 AGT20491.1     27
 amylosucrase (Amas) 2.4.1.4 Alteromonas macleodii KCTC 2957 BAG82876.1 B6F2H1   4
 pullulanase (PulAg) 3.2.1.41 Anaerobranca gottschalkii AAS47565.1 Q6QHE9   14
 β-cyclodextrin glycosyltransferase (CGTase) 2.4.1.19 Anaerobranca gottschalkii CAH61550.1 Q5ZEQ7   2
 α-amylase (AmyB) 3.2.1.1 Anaerobranca gottschalkii AAW32490.1
CAZ78873.1
Q5I943  
 α-amylase (AmyA) 3.2.1.1 Anaerobranca gottschalkii AAW32491.1 Q5I942   36
 1,4-α-glucan branching enzyme (GlgB) 2.4.1.18 Anaerobranca gottschalkii CAJ38414.1 Q3BJY5   9
 pullulanase 3.2.1.41 Anaerobranca horikoshii AAP45012.1 Q6XQ00   14
 cyclomaltodextrinase (Cda13;Amy98) 3.2.1.54 Anoxybacillus flavithermus AAX29991.1
CAY02963.1
CAZ78871.1
Q5BLZ6   20
 α-amylase (AFA;AMY1048) 3.2.1.1 Anoxybacillus flavithermus CAL14744.1     5
 maltogenic α-amylase (ADTA) 3.2.1.133 Anoxybacillus sp. DT3-1 AFI49456.1    
 type I pullulanase (PulA) 3.2.1.41 Anoxybacillus sp. LM18-11 AEW23439.1   3WDH[A]
3WDI[A]
3WDJ[A]
14
 α-amylase (ASKA) 3.2.1.1 Anoxybacillus sp. SK3-4 AFI49455.1   5A2A[A]
5A2B[A]
5A2C[A]
 branching enzyme (GlgB;Aq_722) 2.4.1.18 Aquifex aeolicus VF5 AAC06895.1
CAE51854.1
NP_213496.1
O66936   9
 cyclic maltosyl-maltose hydrolase (CmmF) 3.2.1.- Arthrobacter globiformis M6 BAI67607.1 D2YYE1   20
 6-α-maltosyltransferase (CmmA) 2.4.1.- Arthrobacter globiformis M6 BAE79266.1 Q2L6M1  
 α-glucosidase (CmmB) 3.2.1.20 Arthrobacter globiformis M6 BAI67603.1
BAE79267.1
D2YYD7
Q2L6M0
  30
 malto-oligosyltrehalose synthase (MTSase;TreY) 5.4.99.15 Arthrobacter ramosus S34 BAB40765.1 Q9AJN7   26
 malto-oligosyltrehalose trehalohydrolase (MTHase;TreZ) 3.2.1.141 Arthrobacter ramosus S34 BAB40766.1 Q9AJN6   10
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Arthrobacter sp. Q36 BAA09667.1
BAA94843.1
Q44315   26
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141 Arthrobacter sp. Q36 AAE13738.1
AAE13730.1
AAE15470.1
AAE15478.1
BAA09668.1
Q44316   10
 α-amylase (fragment) 3.2.1.1 Bacillus acidicola TSAS1 AEW07376.1     5
 pullulanase (PulB) 3.2.1.41 Bacillus acidopullulyticus CAC60158.1
AAX00709.1
ACK18365.1
CAX87016.1
CAZ67879.1
CAZ67880.1
    14
 pullulanase (PulA;BaPul13A) (Pul13A) 3.2.1.41 Bacillus acidopullulyticus CAC60156.1
AAX00707.1
ACK18363.1
CAX87014.1
  2WAN[A] 14
 maltogenic α-amylase 3.2.1.133 Bacillus acidopullulyticus CAA80246.1
CAY02968.1
CAZ78895.1
P32818   20
 α-amylase (Amy1;AmyQ;BAA) 3.2.1.1 Bacillus amyloliquefaciens AAA22191.1
AAE12381.1
AAE13947.1
AAE37101.1
AAE59727.1
AAE62349.1
AAN20003.1
AAN27445.1
AAN99911.1
AAP02211.1
AAR63623.1
AAR76030.1
AAS26291.1
AAY72173.1
ABJ43095.1
ABJ43099.1
ABN29369.1
ACK07344.1
ACP75069.1
ACS10035.1
ACW26904.1
CAA01489.1
CAA23430.1
CAC16484.1
CAC88551.1
CAD19035.1
CAD20678.1
CAD26702.1
CAD67517.1
CAD88112.1
P00692 3BH4[A,B] 5
 α-amylase 3.2.1.1 Bacillus amyloliquefaciens ATCC 23842 ADE44086.1 D5KR60   5
 α-amylase (BaqA) 3.2.1.1 Bacillus aquimaris MKSC 6.2 AER68125.1    
 branching-enzyme (GlgB) 2.4.1.18 Bacillus caldolyticus CAA78440.1 P30537   9
 oligo-α-1,6-glucosidase (BC4015) 3.2.1.10 Bacillus cereus ATCC 14579 AAP10934.1
NP_833733.1
Q819G7   31
 oligo-α-1,6-glucosidase (MalL) 3.2.1.10 Bacillus cereus ATCC 7064 CAA37583.1 P21332 1UOK[A] 31
 α-amylase (BCA) 3.2.1.1 Bacillus cereus GUF8 ABY86223.1 B0LVG1   5
 type II pullulanase A (PulA) 3.2.1.41 Bacillus cereus GXBC-3 AFP89962.1     14
 type I pullulanase C (PulC) 3.2.1.41 Bacillus cereus GXBC-3 AFP89964.1     14
 type I pullulanase B (PulB) 3.2.1.41 Bacillus cereus GXBC-3 AFP89963.1     14
 pullulanase 3.2.1.41 Bacillus cereus OPF-0031 AFJ04510.1     14
 branching enzyme 2.4.1.18 Bacillus cereus Q BAE96028.1 Q1AQI6   9
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus circulans 251 CAA55023.1 P43379 1CDG[A]
1CGV[A]
1CGW[A]
1CGX[A]
1CGY[A]
1CXE[A]
1CXF[A]
1CXH[A]
1CXI[A]
1CXK[A]
1CXL[A]
1D3C[A]
1DTU[A]
1EO5[A]
1EO7[A]
1KCK[A]
1KCL[A]
1OT1[A]
1OT2[A]
1PEZ[A]
1PJ9[A]
1TCM[A,B]
2CXG[A]
2DIJ[A]
2
 β-cyclodextrin glucanotransferase (CGTase) 2.4.1.19 Bacillus circulans 8 CAA48401.1 P30920 1CGT[A]
1CGU[A]
3CGT[A]
4CGT[A]
5CGT[A]
6CGT[A]
7CGT[A]
8CGT[A]
9CGT[A]
2
 α-1,6-cyclomaltopentaose-forming glucanotransferase (IgtY) 2.4.1.- Bacillus circulans AM7 BAF37283.1 A0P8W9  
 a/β-cyclodextrin glycosyltransferase 2.4.1.19 Bacillus circulans DF 9R ACF42340.2
ACF42340.1
C4MH58   2
 α-amylase 2.4.1.19
3.2.1.1
Bacillus circulans F-2 AAA22229.1 P08137   2
 β-cyclodextrin glycosyltransferase 2.4.1.19 Bacillus circulans STB01 AIS84204.1     2
 g-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus clarkii 7364 BAH14968.1
ABC06412.1
BAB91217.1
BAB91217.2
CAD87625.1
B9A1J6
Q8L3E0
4JCM[A] 2
 g-cyclodextrin-specific cyclodextrinase (Cda) 3.2.1.54 Bacillus clarkii 7364 BAH14969.1 B9A1J7   36
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