Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); amino acid transporter; [retaining] sucrose 6(F)-phosphate phosphorylase (EC 2.4.1.329); [retaining] glucosylglycerol phosphorylase (EC 2.4.1.359); ; Glucosylglycerate phosphorylase (EC 2.4.1.352); [retaining] sucrose α-glucosidase (EC 3.2.1.48); oligosaccharide α-4-glucosyltransferase (EC 2.4.1.161); [retaining] α-amylase (EC 3.2.1.1);
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (141506); Uniprot accession (1021); PDB accession (624); 3D entries (148); cryst (2)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
Archaea
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 pullulanase (ApuA;Pul-Dm;Desmu_0653) 3.2.1.41   Desulfurococcus mucosus DSM 2162 AAG31033.1
ADV64961.1
E8R8Y5
Q9HHB0
  20
 α-amylase (AmyH) 3.2.1.1 pubmed
Haloarcula hispanica DSM 4426 CAI64586.1 Q4A3E0   43
 α-amylase (MalA) 3.2.1.1   Haloarcula japonica TR-1 BAM75337.1 L8B068  
 α-cyclomaltodextrin glucanotransferase (Cgt) 2.4.1.19   Haloferax mediterranei CAI46245.1 Q53I75   2
 maltooligosyltrehalose trehalohydrolase (TreZ;MTH) 3.2.1.141   Metallosphaera hakonensis JCM 8857 AAR18372.1 Q6SZP6   10
 maltooligosyltrehalose synthase (TreY;MTS) 5.4.99.15   Metallosphaera hakonensis JCM 8857 AAR18371.1 Q6SZP7   26
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116
pubmed
Natronococcus sp. Ah-36 BAA05516.1 Q60224   43
 trehalose synthase (PTO0069) 5.4.99.16   Picrophilus torridus DSM 9790 AAT42654.1
CBF79071.1
Q6L2Z7   16
 α-amylase / cyclomaltodextrinase (PFTA; PF1939) (Amy13) 3.2.1.1
3.2.1.54
pubmed
pubmed
Pyrococcus furiosus DSM 3638 AAL82063.1
CAZ78721.1
NP_579668.1
Q8TZP8 4AEF[A,B] 20
 β-cyclodextrin glucanotransferase (PfCgt;PF0478;PF0478*) 2.4.1.19   Pyrococcus furiosus DSM 3638 ABA33720.1
AAL80602.1
ABA30024.1
ABA30025.1
NP_578207.1
Q3HUR2
Q8U3I8
  2
 α-amylase (AmyA; PFA; PF0477) 3.2.1.1 pubmed
Pyrococcus furiosus DSM 3638 NP_578206.1
AAB67705.1
AAC45663.1
AAL80601.1
O08452
Q8U3I9
  7
 α-amylase (AmyA) 3.2.1.1   Pyrococcus woesei AAD54338.1
AAF44693.1
ACH26211.1
Q7LYT7
Q9P9L0
1MWO[A]
1MXD[A]
1MXG[A]
7
 glycogen debranching enzyme (TreX) 2.4.1.25
3.2.1.68
  Saccharolobus shibatae B12 AAM81590.1
QXJ29377.1
Q8NKQ3   11
 maltooligosyltrehalose synthase (SsTDFE; TreY; J5U23_02247) 5.4.99.15 pubmed
Saccharolobus shibatae B12 AAM81591.1
AAF17554.1
QXJ29378.1
Q7LYV2
Q9UWN8
5ZCR[A,B] 26
 maltooligosyl trehalose trehalohydrolase (SsTFE ; TreZ; J5U23_02244) 3.2.1.141 pubmed
Saccharolobus shibatae B12 AAM81589.1
AAF17553.1
QXJ29375.1
Q7LYV3
Q9UWN9
  10
 α-amylase / maltooligosyltrehalose trehalohydrolase (GTHase) 3.2.1.1
3.2.1.141
  Saccharolobus solfataricus KM1 AAR46454.1
BAA11010.1
Q55088 1EH9[A]
1EHA[A]
3VGB[A]
3VGD[A]
3VGE[A]
3VGF[A]
3VGG[A]
3VGH[A]
10
 glycosyltrehalose-producing enzyme 5.4.99.15   Saccharolobus solfataricus KM1 BAA11008.1 Q55262   26
 maltooligosyltrehalose synthase (TreY;SSO2095) 5.4.99.15   Saccharolobus solfataricus P2 AAK42274.1
CAA69505.1
NP_343484.1
P95869
Q7LX98
  26
 maltooligosyltrehalose trehalohydrolase (TreZ;MTHase;SSO2093;c06020) 3.2.1.141   Saccharolobus solfataricus P2 AAK42272.1
CAA69503.1
CAC23737.1
NP_343482.1
P95867   10
 isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25
3.2.1.68
  Saccharolobus solfataricus P2 AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868
Q7LX99
2VNC[A,B]
2VR5[A,B]
2VUY[A,B]
7EAV[A,B]
11
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) 3.2.1.133
3.2.1.54
  Staphylothermus marinus F1 ABN69720.1 A3DM60 4AEE[A,B] 20
 α-amylase/maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.1
3.2.1.141
  Sulfolobus acidocaldarius BAA11011.1
BAA11863.1
Q53641   10
 maltooligosyltrehalose synthase (TreY;MTSase) 5.4.99.15   Sulfolobus acidocaldarius BAA11009.1
BAA11865.1
Q53688 1IV8[A] 26
 isoamylase (TreX) 3.2.1.68   Sulfolobus acidocaldarius ATCC33909 BAA11864.1 O05152   11
 pullulanase type III (PulhA;Pul-Ta) 3.2.1.41   Thermococcus aggregans CAB94218.1
CAZ78888.1
Q9P9A0   20
 cyclomaltodextrinase (CD;TcCD;CL1_0884) 3.2.1.54   Thermococcus cleftensis AFL95089.1 I3ZTQ5   20
 α-amylase (Amy) 3.2.1.1   Thermococcus hydrothermalis AL662 AAC97877.1
AAC97878.1
ACH26213.1
O93647
O93648
  7
 β-cyclodextrin glucanotransferase (Cgt;TK-cgt;TK2172) 2.4.1.19   Thermococcus kodakarensis KOD1 BAB78538.1
BAD86361.1
CAT70763.1
Q8X268   2
 cyclodextrinase (CDase-Tk;TK1770) 3.2.1.54   Thermococcus kodakarensis KOD1 BAD85959.1
CAT70564.1
Q5JJ59   20
 amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977) 3.2.1.1
3.2.1.41
  Thermococcus kodakarensis KOD1 BAD85166.1
CAT69621.1
CAZ78793.1
Q5JID9 5OT1[A] 20
 α-amylase (ApkA;TK1884) 3.2.1.1 doi
Thermococcus kodakarensis KOD1 BAA21130.1
ACH26210.1
BAD86073.1
CAT70635.1
O33476
Q5JER7
  7
 cyclomaltodextrinase (Tocd;TON_1796) 3.2.1.54   Thermococcus onnurineus NA1 ACJ17286.1 B6YV58   20
 α-amylase (TO_Amyl;TON_1660) 3.2.1.1   Thermococcus onnurineus NA1 ABA26948.1
ACJ17150.1
B6YUG5
Q2QC88
  7
 α-amylase (BD5031) 3.2.1.1   Thermococcus sp. AEPII 1a AAM48113.1
ACH26099.1
Q8NKR4   7
 α-cyclodextrin glucanotransferase (CgtA) 2.4.1.19   Thermococcus sp. B1001 BAA88217.1
BAB18101.1
CAZ78889.1
Q9UWN2   2
 cyclomaltodextrinase (CdnA) 3.2.1.133
3.2.1.54
  Thermococcus sp. B1001 BAB18100.1
CAZ78887.1
Q9HHC8   20
 α-amylase (BD5064) 3.2.1.1   Thermococcus sp. GU5L5 AAM48112.1
ACH26098.1
Q8NKR5   7
 α-amylase (THJA) 3.2.1.1   Thermococcus sp. HJ21 ABU98335.1 B4X9V8   7
 α-amylase 3.2.1.1   Thermococcus sp. Rt3 AAB87860.1
ACH26212.1
O50200   7
 α-amylase (Amy) 3.2.1.1   Thermococcus thioreducens AAT11125.1 Q66SB4   7
 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458) 3.2.1.133
3.2.1.54
  Thermofilum pendens Hrk 5 ABL78855.1 A1S075   20
 maltogenic α-amylase (TpMA;TVG0226058) 3.2.1.133   Thermoplasma volcanium GSS1 BAB59359.1
CAZ78693.1
NP_110736.1
Q97C86   20
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 acarbose resistent α-amylase (AcbE;ACPL_3683) 3.2.1.1   Actinoplanes sp. SE50/110 AEV84578.1
CAC02970.1
CAJ81030.1
SLM00378.1
G8SLW4
Q27GR6
Q9K5L6
 
 acarviosyltransferase (AcbD; ACPL_3684) 2.4.1.-   Actinoplanes sp. SE50/110 CAC02971.1
AAE37556.1
AEV84579.1
CAJ81031.1
SLM00379.1
Q27GR5
Q9K5L5
  2
 trehalose synthase (TreS) 5.4.99.16   Actinoplanes sp. SN223/29 ABV26719.1 A8QX00   16
 malto-oligosyltrehalose synthase (TreY) 5.4.99.15   Actinoplanes sp. SN223/29 ABV26725.1 A8QX07   26
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141   Actinoplanes sp. SN223/29 ABV26726.1 A8QX08   10
 α-amylase 3.2.1.1 pubmed
Aeromonas hydrophila JMP636 AAA21936.1 P22630   27
 α-amylase (AmyA) 3.2.1.1 pubmed
Aeromonas hydrophila MCC-1 AAA21016.1 P41131   32
 amylase-pullulanase (Amy;Aaci_2874) 3.2.1.1
3.2.1.41
pubmed
pubmed
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59878.1
CAA44638.1
CAZ78868.1
C8WUR2
Q06307
  39
 cyclomaltodextrinase (CdaA;Aaci_2869) 3.2.1.54 pubmed
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ACV59873.1
CAB40078.1
CAB65655.1
CAY02967.1
CAZ78890.1
C8WUQ7
Q9WX32
  20
 α-amylase 3.2.1.1   Alicyclobacillus sp. 18711 AWX66236.1 A0A3P8MUS3 6GXV[A,B]
6GYA[A,B,C,D]
5
 α-amylase (AmyA4) 3.2.1.1   Alicyclobacillus sp. A4 / CGMCC 3147 ADZ99363.1 F2VRZ2   39
 maltogenic α-amylase (Mag1;BleG1_0772) 3.2.1.54   Alkalihalobacillus lehensis G1 AHW51935.1
AIC93380.1
X5CPN2   20
 α-glucosidase (Amy112) (partial) 3.2.1.21 doi
Alkalihalophilus pseudofirmus 703 AQS95354.1     31
 α-amylase (Amy703;Amy) 3.2.1.1   Alkalihalophilus pseudofirmus 703 AGT54942.1 A0A023I4U3   20
 α-amylase (Amy1;AmyA;AmyB) 3.2.1.1   Alkalimonas amylolytica N10 AAQ01675.1 Q6WUB6   5
 α-amylase (AmySTU) 3.2.1.1   Alteromonas macleodii B7 AGT20491.1 V9P2Q6   27
 amylosucrase (Amas) 2.4.1.4   Alteromonas macleodii KCTC 2957 BAG82876.1 B6F2H1   4
 pullulanase (PulAg) 3.2.1.41   Anaerobranca gottschalkii AAS47565.1 Q6QHE9   14
 β-cyclodextrin glycosyltransferase (CGTase) 2.4.1.19   Anaerobranca gottschalkii CAH61550.1 Q5ZEQ7   2
 α-amylase (AmyB) 3.2.1.1   Anaerobranca gottschalkii AAW32490.1
CAZ78873.1
Q5I943  
 α-amylase (AmyA) 3.2.1.1   Anaerobranca gottschalkii AAW32491.1 Q5I942   36
 1,4-α-glucan branching enzyme (GlgB) 2.4.1.18   Anaerobranca gottschalkii CAJ38414.1 Q3BJY5   9
 pullulanase 3.2.1.41   Anaerobranca horikoshii AAP45012.1 Q6XQ00   14
 cyclomaltodextrinase (Cda13;Amy98) 3.2.1.54   Anoxybacillus flavithermus AAX29991.1
CAY02963.1
CAZ78871.1
Q5BLZ6   20
 α-amylase (AFA;AMY1048) 3.2.1.1   Anoxybacillus flavithermus CAL14744.1     5
 maltogenic α-amylase (ADTA) 3.2.1.133 pubmed
Anoxybacillus sp. DT3-1 AFI49456.1 I1VWI0  
 α-amylase (AGXA) 3.2.1.1 pubmed
Anoxybacillus sp. GXS-BL AEQ38578.1    
 type I pullulanase (PulA) 3.2.1.41   Anoxybacillus sp. LM18-11 AEW23439.1 K9L0H1 3WDH[A]
3WDI[A]
3WDJ[A]
14
 α-amylase (ASKA) 3.2.1.1 pubmed
Anoxybacillus sp. SK3-4 AFI49455.1 I1VWH9 5A2A[A]
5A2B[A]
5A2C[A]
 branching enzyme (GlgB;Aq_722) 2.4.1.18 doi
Aquifex aeolicus VF5 AAC06895.1
CAE51854.1
NP_213496.1
O66936   9
 cyclic maltosyl-maltose hydrolase (CmmF) 3.2.1.-   Arthrobacter globiformis M6 BAI67607.1 D2YYE1 5ZXG[A,B]
6A0J[A]
6A0K[A,B]
6A0L[A]
20
 6-α-maltosyltransferase (CmmA) 2.4.1.-   Arthrobacter globiformis M6 BAE79266.1 Q2L6M1  
 α-glucosidase (CmmB) 3.2.1.20   Arthrobacter globiformis M6 BAI67603.1
BAE79267.1
D2YYD7
Q2L6M0
  30
 malto-oligosyltrehalose synthase (MTSase;TreY) 5.4.99.15   Arthrobacter ramosus S34 BAB40765.1 Q9AJN7 6LCU[A]
6LCV[A]
26
 malto-oligosyltrehalose trehalohydrolase (MTHase;TreZ) 3.2.1.141   Arthrobacter ramosus S34 BAB40766.1 Q9AJN6   10
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 pubmed
Arthrobacter sp. Q36 BAA09667.1
BAA94843.1
Q44315
Q9LC79
  26
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141   Arthrobacter sp. Q36 AAE13738.1
AAE13730.1
AAE15470.1
AAE15478.1
BAA09668.1
Q44316   10
 α-amylase (fragment) 3.2.1.1   Bacillus acidicola TSAS1 AEW07376.1 J9PQD2   5
 pullulanase (PulB) 3.2.1.41   Bacillus acidopullulyticus CAC60158.1
AAX00709.1
ACK18365.1
CAX87016.1
CAZ67879.1
CAZ67880.1
    14
 pullulanase (PulA;BaPul13A) (Pul13A) 3.2.1.41   Bacillus acidopullulyticus CAC60156.1
AAX00707.1
ACK18363.1
CAX87014.1
  2WAN[A] 14
 α-amylase (Amy1;AmyQ;BAA) 3.2.1.1 pubmed
Bacillus amyloliquefaciens AAA22191.1
AAE12381.1
AAE13947.1
AAE37101.1
AAE59727.1
AAE62349.1
AAN20003.1
AAN27445.1
AAN99911.1
AAP02211.1
AAR63623.1
AAR76030.1
AAS26291.1
AAY72173.1
ABJ43095.1
ABJ43099.1
ABN29369.1
ACK07344.1
ACP75069.1
ACS10035.1
ACW26904.1
CAA01489.1
CAA23430.1
CAC16484.1
CAC88551.1
CAD19035.1
CAD20678.1
CAD26702.1
CAD67517.1
CAD88112.1
P00692 3BH4[A,B] 5
 α-amylase 3.2.1.1   Bacillus amyloliquefaciens ATCC 23842 ADE44086.1 D5KR60   5
 branching-enzyme (GlgB) 2.4.1.18   Bacillus caldolyticus CAA78440.1 P30537   9
 oligo-α-1,6-glucosidase (MalL) 3.2.1.10 pubmed
Bacillus cereus ATCC 7064 CAA37583.1 P21332 1UOK[A] 31
 α-amylase (BCA) 3.2.1.1   Bacillus cereus GUF8 ABY86223.1 B0LVG1   5
 type II pullulanase A (PulA) 3.2.1.41   Bacillus cereus GXBC-3 AFP89962.1 J7H570   14
 type I pullulanase C (PulC) 3.2.1.41   Bacillus cereus GXBC-3 AFP89964.1 J7H7V3   14
 type I pullulanase B (PulB) 3.2.1.41   Bacillus cereus GXBC-3 AFP89963.1 J7H256   14
 pullulanase 3.2.1.41   Bacillus cereus OPF-0031 AFJ04510.1 R4I4I6   14
 branching enzyme 2.4.1.18   Bacillus cereus Q BAE96028.1 Q1AQI6   9
 amylopullulanase (PulA) (partial) 3.2.1.41   Bacillus deramificans T89.117D AAE10887.1     14
 pullulanase (BFLpu) 3.2.1.41   Bacillus flavocaldarius KP1228 BAB18516.1 Q9F236   20
 oligo-α-1,6-glucosidase 3.2.1.10   Bacillus flavocaldarius KP1228 BAB18518.1
CBF79207.1
Q9F237   23
 Sequence from patent 3.2.1.1   Bacillus licheniformis AAE13946.1
AAE37100.1
AAE59726.1
AAE62348.1
AAN27444.1
AAP02210.1
AAR63628.1
AAR76029.1
AAS26290.1
ABA38471.1
ABJ43094.1
ABN29368.1
ACK07343.1
ACP75068.1
ACS10034.1
ACW26903.1
CAD38134.1
CAD67516.1
    5
 α-amylase (Am-Lich) 3.2.1.1   Bacillus licheniformis AAE12380.1     5
 α-amylase 3.2.1.1   Bacillus licheniformis AAE12379.1
ABA38472.1
ACS10033.1
    5
 a/β-cyclodextrin glucanotransferase (CgtA) 2.4.1.19 pubmed
Bacillus licheniformis CAA33763.1 P14014   2
 maltogenic α-amylase (BLMA) (frameshift) 3.2.1.133 pubmed
Bacillus licheniformis CAA47612.1 Q04977   20
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