Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (55939); Uniprot accession (7858); PDB accession (467); 3D entries (111); cryst (2)
All (51170) Archaea (370) Bacteria (47636) Eukaryota (3034) unclassified (130) Structure (111 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 α-amylase (Amy63) 3.2.1.1 Vibrio alginolyticus 129-63 ALP73597.1     5
 glycogen branching enzyme (GBE;VvGBE;VVM_00156;VVMO6_03058) 2.4.1.18 Vibrio vulnificus MO6-24/O ADV88080.1     9
 sucrose hydrolase (XAC3490) 3.2.1.- Xanthomonas axonopodis pv. citri str. 306 AAM38333.1
NP_643797.1
Q8PGX2   4
 sucrose hydrolase (Suh) 3.2.1.- Xanthomonas axonopodis pv. glycines 8RA AAQ93678.1 Q6UVM5 3CZE[A]
3CZG[A]
3CZK[A]
3CZL[A]
4
 α-amylase 3.2.1.1 Xanthomonas campestris AAA27591.1
AAO62080.1
Q56791
Q847N0
  27
 α-amylase 3.2.1.1 Xanthomonas campestris K-11151 BAA07401.1
CAZ78877.1
CBF79165.1
Q60102   36
 α-amylase (Amy) 3.2.1.1 Xanthomonas campestris pv. campestris str. ATCC 33913 AAM40063.1
NP_636139.1
Q7CLU8   27
 sucrose hydrolase (SuxB;XCC3359) 3.2.1.- Xanthomonas campestris pv. campestris str. ATCC 33913 AAM42629.1
NP_638705.1
Q8P5I2 2WPG[A] 4
 α-transglucosidase (XgtA) 2.4.1.- Xanthomonas campestris WU-9701 BAC87873.1
CBF79179.1
Q76LB0   23
 α-1,4-glucan branching enzyme (GlgB;YPO3942) 2.4.1.18 Yersinia pestis CO92 CAC93404.1
CAL22523.1
NP_407383.1
Q8ZA75   9
 α-1,4-glucan branching enzyme (GlgB;y3886) 2.4.1.18 Yersinia pestis KIM10+ AAM87431.1
NP_671180.1
    9
 α-amylase (AmyZ2;ZPR_2815) 3.2.1.1 Zunongwangia profunda SM-A87 ADF53135.1 D5BG22  
 α-amylase (AmyZ;ZPR_2816) 3.2.1.1 Zunongwangia profunda SM-A87 ADF53136.1 D5BG23  
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 α-amylase / allergen (Aca s 4) 3.2.1.1 Acarus siro ABL09312.1 B0KZK1   24
 sucrase / α-glucosidase (S1;APS1) 3.2.1.20 Acyrthosiphon pisum ABB55878.1 Q0H3F1   17
 α-amylase I (AmyI) 3.2.1.1 Aedes aegypti AAA29343.1
AAA29344.1
AAA29345.1
AAB60935.1
O02413
P53354
  15
 α-amylase II (AmyII) 3.2.1.1 Aedes aegypti AAB60934.1 O02652   15
 α-amylase 3.2.1.1 Aedes atropalpus AAA03322.1 Q16924   15
 isoamylase-type starch debranching enzyme 3 (Isa3;CrISA3) 3.2.1.68 Amaranthus hybridus subsp. cruentus BAQ22171.1     11
 α-amylase (Amy) 3.2.1.1 Anguilla japonica BAB85635.1 Q8QGW2   24
 α-amylase (Aamy1) 3.2.1.20 Anopheles albimanus ADD81256.1 D4N3A2   17
 α-glucosidase (Agm1) 3.2.1.20 Anopheles gambiae G3 CAA60857.1 Q17021   17
 α-glucosidase (Agm2) 3.2.1.20 Anopheles gambiae G3 CAA60858.1
CBF79157.1
Q17022   17
 α-glucosidase / binding-toxin receptor (Agm3;AgAgm3) 3.2.1.20 Anopheles gambiae G3 ABW98683.1 A9YDV2   17
 α-amylase 3.2.1.1 Anopheles merus AAA03323.1 Q17059   15
 α-amylase (Amylag1) 3.2.1.1 Anthonomus grandis AAN77138.1
AOG18226.1
Q8I9K6   15
 α-amylase (Amylag2) 3.2.1.1 Anthonomus grandis AAN77139.1 Q8I9K5   15
 α-glucosidase 3.2.1.20 Apis cerana indica ABO27432.1 A4GVI6   17
 sucrase / α-glucosidase II (JBgII;HBGaseII) 3.2.1.20
3.2.1.-
Apis cerana japonica ACN63343.1 C0LQJ2   17
 α-glucosidase I (JBgI) 3.2.1.20 Apis cerana japonica BAF44218.1 A1IHL0   17
 α-glucosidase III 3.2.1.20 Apis dorsata ADB11049.1 D2XNP9   17
 α-glucosidase III (Hbg3;HBG-III;Loc406131) 3.2.1.20 Apis mellifera BAA11466.1
BAE86928.1
CBF79234.1
NP_001011608.1
XP_392790.1
Q17058 cryst 17
 α-glucosidase I (Hbg1;HBG-I;Loc409889) 3.2.1.20 Apis mellifera BAE86926.1
XP_393379.2
Q25BT8   17
 α-glucosidase II (Hbg2;HBG-II;Loc411257) 3.2.1.20 Apis mellifera BAE86927.1
CBF79049.1
XP_394730.1
Q25BT7   17
 pullulanase / isoamylase (Lda;AtPU1;AtLDA;LDA1;At5g04360) 3.2.1.41
3.2.1.68
Arabidopsis thaliana AAO00771.1
AED90732.1
ANM68325.1
NP_196056.1
Q8GTR4   13
 branching-enzyme 2 (BE2;Sbe2-1;Sbe2.1;At2g36390) 2.4.1.18 Arabidopsis thaliana AAD24644.1
AAB03099.1
AAM97077.1
ABC04873.1
AEC09247.1
BAE98973.1
CAA04134.1
NP_181180.1
O23647
Q42526
  8
 branching-enzyme 3 (BE3;sbe2-2;sbe2.2;At5g03650) 2.4.1.18 Arabidopsis thaliana CAB82930.1
AAB03100.1
ABC04874.1
AED90637.1
BAC42378.1
NP_195985.1
Q42531
Q8GYC4
Q9LZS3
  8
 α-amylase (AtAmy1;At4g25000) (apoplast) 3.2.1.1 Arabidopsis thaliana CAB36742.1
AAL38709.1
AAM51369.1
AAM64582.1
AEE84990.1
CAB79409.1
NP_567714.1
Q8LBS5
Q8VZ56
Q9SW26
  6
 At1g03310 (chloroplastic isoamylase 2;AtISA2;Iso1 subunit;DBE1) 3.2.1.68 Arabidopsis thaliana AAC72113.1
AAM98123.1
AEE27557.1
AEE27558.1
NP_171830.1
Q8L735
Q9ZVT2
  11
 At2g39930 (chloroplastic isoamylase 2;AtISA1;Iso1 subunit) 3.2.1.68 Arabidopsis thaliana AAB95278.1
AAN17420.1
AAQ56791.1
AEC09752.1
ANM62439.1
ANM62440.1
NP_181522.1
O04196   11
 chloroplastic isoamylase 3 (Isa3;AtISA3;At4g09020) 3.2.1.68 Arabidopsis thaliana CAB78026.1
AAM13879.1
AAM91673.1
AEE82713.1
BAE99109.1
NP_192641.1
Q8RWW6
Q9M0S5
  11
 branching-enzyme 1 (Be1;AtSBEIII;At3g20440) (AtBEI) 2.4.1.18 Arabidopsis thaliana BAC43378.1
ADB29066.1
AEE76378.1
AEE76379.1
AEE76380.1
BAB02827.1
NP_188679.1
D2WL32
Q8GWK4
Q9LTP8
  8
 α-amylase (Amyl III) 3.2.1.1 Aspergillus awamori KT-11 BAD06003.1
BAD06005.1
Q76L96   1
 α-amylase (Amyl I) 3.2.1.1 Aspergillus awamori KT-11 BAD06002.1 Q76L99   1
 α-amylase (Amy1;TAA) 3.2.1.1 Aspergillus flavus AAF14264.1
AAS89853.1
AAS89854.1
AAS89855.1
AAS89856.1
AAS89857.1
AAS89858.1
AAS89859.1
AAS89860.1
AAS89861.1
AAS89862.1
AAS89863.1
AAS89864.1
AAS89865.1
AAS89866.1
AAS89867.1
AAS89868.1
AAS89869.1
AAS89870.1
AAS89871.1
AAS89872.1
AAS89873.1
AAS89874.1
AAS89875.1
AAS89876.1
AAS89877.1
AAS89878.1
AAS89879.1
AAS89880.1
AAS89881.1
AAS89882.1
AAS89883.1
AAS89884.1
AAS89885.1
AAS89886.1
AAS89887.1
AAS89888.1
AAS89889.1
AAS89890.1
AAS89891.1
AAS89892.1
Q7LV45
Q96TH4
  1
 α-1,3-glucan synthase (Ags1) 2.4.1.183 Aspergillus fumigatus AAL28129.1 Q96UQ6   22
 α-1,3-glucan synthase (AGS3;Afu1g15440) 2.4.1.183 Aspergillus fumigatus Af293 EAL90874.1 Q4WRQ8   22
 α-amylase (AmyA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAD01051.1 Q76CT3   1
 α-amylase (AsaA) 3.2.1.1 Aspergillus kawachii IFO 4308 BAA22993.1
CAX51628.1
O13296   1
 α-1,3-glucan synthase (AgsA;AN5885;ANID5885) 2.4.1.183 Aspergillus nidulans FGSC A4 EAA58394.1 C8UZW7
Q5B0P5
  22
 α-1,3-glucan synthase (AgsB;AN3307;ANID3307) 2.4.1.183 Aspergillus nidulans FGSC A4 EAA63275.1 C8VHZ4
Q5B823
  22
 α-amylase (AmyA;AN2018.2) 3.2.1.1 Aspergillus nidulans FGSC A4 EAA64850.1
AAF17103.1
C8VLE6
G5EB45
  1
 α-amylase (AmyB;AN3402.2) 3.2.1.1 Aspergillus nidulans FGSC A4 AAF17100.1
EAA63370.1
C8VHL8
G5EAT0
Q9UV09
  1
 α-amylase (partial) 3.2.1.1 Aspergillus niger   P56271 2AAA[A] 1
 α-amylase A / B (AmyA;AmyB;An05g02100) 3.2.1.1 Aspergillus niger CBS 513.88 CAK44871.1
CAK46324.1
CAL33800.1
A2QL05
A2QL05
2GUY[A]
2GVY[A,B]
1
 acid α-amylase (AamA;An11g03340) 3.2.1.1 Aspergillus niger CBS 513.88 CAL15439.1
A35282
ADX42122.1
CAK48325.1
CAO78410.1
CAX51630.1
A2QW02   1
 maltotriose-producing α-amylase (AmyD;An01g13610) (intracellular) 3.2.1.1
3.2.1.116
Aspergillus niger CBS 513.88 CAK37367.1 A2QB23   5
 cell-wall 4-α-glucanotransferase (AgtA;An09g03100) 2.4.1.25 Aspergillus niger CBS 513.88 CAK40249.1 A2QTS4   1
 4-α-glucanotransferase (AgtB;An12g02460) 2.4.1.25 Aspergillus niger CBS 513.88 CAK41088.1 A2QYT9   1
 α-1,3-glucan synthase (AgsA) 2.4.1.183 Aspergillus niger N402 CAD88949.1
AAS99324.1
    22
 α-amylase A (AmyA) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36966.1 Q02905   1
 α-amylase B (AmyB) 3.2.1.1 Aspergillus niger VAR. AWAMORI UVK143F CAA36967.1
CAL15437.1
CAL15438.1
CAT03384.1
Q02906   1
 Sequence from patent 3.2.1.1 Aspergillus oryzae AAE13949.1
AAE37103.1
AAN27447.1
    1
 α-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21) 3.2.1.1 Aspergillus oryzae DSM63303 CAA31218.1
AAA32708.1
ACP34134.1
BAA00336.1
CAA31219.1
CAA31220.1
CAC40348.1
CAT03377.1
CAV35984.1
P0C1B3
P10529
2TAA[A]
3KWX[A]
6TAA[A]
7TAA[A]
1
 glycogen branching enzyme (GbeA;AO090010000483) 2.4.1.18 Aspergillus oryzae RIB40 BAB69770.1
BAE66334.1
Q96VA4   8
 α-amylase (Amy1;AmyI;Taα-G1;AO090023000944;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE59434.1 P0C1B3 3VX0[A] 1
 α-amylase (Amy2;AmyII;Taα-G2;AO090120000196;AmyA;AmyB) 3.2.1.1 Aspergillus oryzae RIB40 BAE62808.1 P0C1B3   1
 α-amylase (Amy3;AmyIII;Taα-G3;AO090003001210;AmyA) 3.2.1.1 Aspergillus oryzae RIB40 BAA95703.1 P0C1B4   1
 α-glucosidase / maltase (MalT;AO090038000234) 3.2.1.20 Aspergillus oryzae RIB40 BAB59003.1
BAE64051.1
CBF79201.1
Q2U314
Q96WT4
  40
 α-1,6-glucosidase / glucodextranase (GlcA) 3.2.1.70 Aspergillus parasiticus SU-1 AAF26276.1
AAS66027.1
CBF79170.1
Q6UEE8   40
 α-amylase (Amy) 3.2.1.1 Aspergillus shirousami BAA01255.1 P30292   1
 α-amylase (AmyA) 3.2.1.1 Aspergillus tubingensis T8.4 AEB80431.1     1
 α-amylase (Amy) 3.2.1.1 Asterias rubens AAO13755.1 Q8IA45   15
 α-amylase A 3.2.1.1 Avena fatua CAA09323.1 O81699   6
 α-amylase D 3.2.1.1 Avena fatua CAA09324.1 O81700   6
 α-amylase (bgtg-1) 3.2.1.1 Blattella germanica AAY23288.1 Q2KJQ1   15
 α-amylase (Ccam) 3.2.1.1 Callosobruchus chinensis BAB72257.1
BAD83655.1
    15
 α-glucosidase (Mal2;Mal1) 3.2.1.20 Candida albicans AAA34350.2
AAQ53127.1
AAV03584.1
C4YN96
Q02751
  40
 glycogen-debranching enzyme (GDE;CgGDE;CAGL0G09977g) 2.4.1.25 Candida glabrata CBS 138 CAG59721.1 Q6FSK0 5D06[A,B]
5D0F[A,B]
25
 isoamylase 1 (Isa1;CrISA1;Sta7) 3.2.1.68 Chlamydomonas reinhardtii CC425 AAP85534.1
AAP88032.1
CAZ78881.1
Q7X8Q2 4J7R[A,B]
4OKD[A,B]
11
 α-amylase B (AmyB;Amy) 3.2.1.1 Crassostrea gigas AAL37207.1
ABB80387.1
ABB80388.1
ABR37235.1
ACC77978.1
ACC77979.1
ACC77980.1
CAD43101.1
CAD43102.1
CAD43103.1
CAD43104.1
Q8I7E4
Q8I7E5
Q8I7E6
Q8I7E7
Q8WSG9
  24
 α-amylase A (AmyA;Amy) 3.2.1.1 Crassostrea gigas AAL37183.1
ACB29736.1
ACC77975.1
ACC77976.1
ACC77977.1
CAA69658.1
CAD43095.1
CAD43096.1
CAD43097.1
CAD43098.1
CAD43099.1
CAD43100.1
O02622
Q8I080
Q8I0T4
Q8I7E8
Q8WSH2
  24
 α-1,3-glucan synthase (Ags1;CNG04420) 2.4.1.183 Cryptococcus neoformans var. neoformans JEC21 AAW44814.1
AAR97368.1
Q5KDD1   22
 α-amylase (Amy-CS2) 3.2.1.1 Cryptococcus sp. S-2 BAA12010.1
BAA12011.1
Q92394   1
 α-glucosidase / maltase 1 (Cpm1) (binary toxin-binding) 3.2.1.20 Culex pipiens AAL05443.1 Q95WY5   17
 α-amylase 3.2.1.1 Culex tarsalis AAA03350.1 Q23767   15
 α-amylase 3.2.1.1 Cuscuta reflexa AAA16513.1 Q42678   6
 α-amylase (Der p 4;Derp4) (allergen) 3.2.1.1 Dermatophagoides pteronyssinus AAD38942.1 P49274
Q9Y197
  24
 α-amylase 3.2.1.1 Diabrotica virgifera virgifera AAF20998.1 Q9U406   15
 α-amylase C1 3.2.1.1 Drosophila ananassae AAC35243.1 O77407   15
 α-amylase (Amy4) 3.2.1.1 Drosophila bocki A65 BAA95436.1 Q9NKZ0   15
 α-amylase (Amy3) 3.2.1.1 Drosophila bocki A65 BAA95435.1 Q9NKZ1   15
 α-amylase (Amy2) 3.2.1.1 Drosophila bocki A65 BAA95434.1 Q9NKZ2   15
 α-amylase (Amy1) 3.2.1.1 Drosophila bocki A65 BAA95433.1 Q9NKZ3   15
 α-amylase (Amy1) 3.2.1.1 Drosophila bocki A94 BAB91544.1 Q9NKZ2   15
 α-amylase (Amy2) 3.2.1.1 Drosophila bocki A94 BAB91547.1 Q8N0P7   15
 α-amylase (Amy1) 3.2.1.1 Drosophila bocki AO-1 BAB91546.1 Q8N0P8   15
 α-amylase (Amy2) 3.2.1.1 Drosophila bocki AO-1 BAB91549.1 Q8N0P5   15
 α-amylase (Amy1) 3.2.1.1 Drosophila bocki IRO2-37 BAB91545.1 Q8N0P9   15
 α-amylase (Amy2) 3.2.1.1 Drosophila bocki IRO2-37 BAB91548.1 Q8N0P6   15
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