Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54252); Uniprot accession (7858); PDB accession (465); 3D entries (110); cryst (2)
All (49476) Archaea (367) Bacteria (45982) Eukaryota (2997) unclassified (130) Structure (110 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 oligo-α-1,6-glucosidase (MalL) 3.2.1.10 Bacillus coagulans ATCC7050 BAA11354.1 Q45101   31
 amylopullulanase (PulA) (partial) 3.2.1.41 Bacillus deramificans T89.117D AAE10887.1     14
 g-cyclodextrin glucanotransferase 2.4.1.19 Bacillus firmus DSM 5850 / 290-3 CAA01436.1     2
 pullulanase (BFLpu) 3.2.1.41 Bacillus flavocaldarius KP1228 BAB18516.1 Q9F236   20
 oligo-α-1,6-glucosidase 3.2.1.10 Bacillus flavocaldarius KP1228 BAB18518.1
CBF79207.1
Q9F237   23
 α-amylase 3.2.1.1 Bacillus halmapalus CAD26699.1
AAE12377.1
AAE59724.1
AAE59730.1
AAE62346.1
AAE62352.1
AAP02208.1
AAP02214.1
AAS26288.1
AAS26294.1
AAY06017.1
AAY72170.1
AAY72176.1
ACK07341.1
ACP75066.1
ACS10037.1
ACW26901.1
CAC88548.1
CAD19032.1
CAD20675.1
CAD67514.1
  1W9X[A]
2GJP[A]
2GJR[A]
5
 α-amylase I (AmyI) (alkaline;thermostable) 3.2.1.1 Bacillus halodurans 38C-2-1 BAF93484.1 A8QWV3   19
 α-amylase G-6 (BH0413) 3.2.1.98 Bacillus halodurans C-125 BAB04132.1
NP_241279.1
Q9KFR4   19
 maltohexaose-forming α-amylase (Amy 34) 3.2.1.98 Bacillus halodurans LBK 34 AAS99099.1 Q5GIA5   19
 maltotetraose-producing α-amylase (AmyA) (alkaline, thermotolerant) 3.2.1.60 Bacillus halodurans MS-2-5 BAG70894.1 B5MEM6   19
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus halodurans US193 CAL25733.1 Q0JW31   2
 maltogenic α-amylase (Mag1;BleG1_0772) 3.2.1.54 Bacillus lehensis G1 AHW51935.1
AIC93380.1
    20
 isoamylase (Iam1) (fragment) 3.2.1.68 Bacillus lentus JNU3 AGL34022.1     14
 Sequence from patent 3.2.1.1 Bacillus licheniformis AAE13946.1
AAE37100.1
AAE59726.1
AAE62348.1
AAN27444.1
AAP02210.1
AAR63628.1
AAR76029.1
AAS26290.1
ABA38471.1
ABJ43094.1
ABN29368.1
ACK07343.1
ACP75068.1
ACS10034.1
ACW26903.1
CAD38134.1
CAD67516.1
    5
 α-amylase (Am-Lich) 3.2.1.1 Bacillus licheniformis AAE12380.1     5
 α-amylase 3.2.1.1 Bacillus licheniformis AAE12379.1
ABA38472.1
ACS10033.1
    5
 a/β-cyclodextrin glucanotransferase (CgtA) 2.4.1.19 Bacillus licheniformis CAA33763.1 P14014   2
 maltogenic α-amylase (BLMA) (frameshift) 3.2.1.133 Bacillus licheniformis CAA47612.1 Q04977   20
 α-amylase (AmyL) 3.2.1.1 Bacillus licheniformis 44MB82/G AGR34077.1     5
 α-amylase (AmyA;AmyL;AmyS;BLA) 3.2.1.1 Bacillus licheniformis 584 / ATCC 27811 AAA22226.1
AAA22193.1
AAA22240.1
AAN99913.1
AAO26743.1
AAT46561.1
ACM17155.1
CAA01355.1
CAA03075.1
CAA26981.1
CAC16483.1
CAC88550.1
CAD19034.1
CAD20677.1
CAD26701.1
CAD26706.1
CAD88111.1
CAV31708.1
P06278
Q6GWE2
1BLI[A]
1BPL[A,B]
1OB0[A]
1VJS[A]
5
 α-amylase (AmyL) 3.2.1.1 Bacillus licheniformis B0204 ABD66592.1 Q208A7   5
 α-amylase (thermostable) 3.2.1.1 Bacillus licheniformis CICC 10181 ACN88150.1 C0LZX2   5
 trehalose-6-P hydrolase (TreA;BlTreA;BLi00797;BL03069) 3.2.1.93 Bacillus licheniformis DSM 13 = ATCC 14580 AAU39732.1
AAU22384.1
CBF79089.1
Q65MI2 5BRP[A,B,C,D]
5BRQ[A,B,C,D]
29
 α-amylase (Amy;BLi00656;BL00499) 3.2.1.1 Bacillus licheniformis DSM 13 = ATCC 14580 AAU39594.1
AAU22245.1
Q65MX0   5
 α-amylase (AmyB) 3.2.1.1 Bacillus licheniformis MD1 CAH10338.1 P06278
Q6GWE2
  5
 α-amylase 3.2.1.1 Bacillus licheniformis NCIB8061 AAE12384.1     5
 α-amylase (AmyN) 3.2.1.1 Bacillus licheniformis NH1 ABL75259.1 A1YR25   5
 maltopentaose-forming α-amylase 3.2.1.- Bacillus megaterium KSM B-404 AAK00598.1 Q9AQ54   5
 α-amylase 3.2.1.1 Bacillus megaterium NCIB 11568 CAA30247.1
ACH26139.1
CAZ78896.1
CBF79230.1
P20845   36
 exo-acting α-amylase (BmaN1) 3.2.1.- Bacillus megaterium NL3 AGT45938.1    
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus ohbensis C-1400 BAA14289.2
I39805
P27036   2
 α-amylase (AmyL;BaLi_c07450) 3.2.1.1 Bacillus paralicheniformis ATCC 9945a AGN35141.1     5
 α-amylase (Amy703;Amy) 3.2.1.1 Bacillus pseudofirmus 703 AGT54942.1     20
 cyclomaltodextrinase (CDI5;BspCMD) 3.2.1.54 Bacillus sp. AAA92925.1 Q59226 1EA9[C] 20
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 1-1   P31746   2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 1011 AAA22308.1 P05618 1D7F[A]
1DED[A]
1I75[A]
1PAM[A]
1UKQ[A,B]
1UKS[A,B]
1UKT[A,B]
1V3J[A,B]
1V3K[A,B]
1V3L[A,B]
1V3M[A,B]
2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 17-1 AAA22310.1 P30921   2
 α-amylase (BAA) 3.2.1.1 Bacillus sp. 195 BAA22082.1 O24781   32
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 20RF AEL33336.1     2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 38-2 AAA22309.1
BAA00077.1
P09121   2
 cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 6.3.3 CAA46901.1 P31747   2
 maltohexaose-forming amylase 3.2.1.98 Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
ACP75071.1
ACS10032.1
ACW26905.1
P19571 1WP6[A]
1WPC[A]
2D3L[A]
2D3N[A]
5
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. 8SB AEL33337.1     2
 cyclodextrinase 3.2.1.54 Bacillus sp. A2-5A BAA31576.1 O82982   20
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. A2-5A BAA31539.1 O82984   2
 α-amylase (AmyL) 3.2.1.1 Bacillus sp. AAH-31 BAN63122.1     20
 β-cyclomaltodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. B1018 AAA22239.1
BAA14140.1
P17692   2
 isoamylase (BsIam) (fragment) 3.2.1.68 Bacillus sp. CICIM 263 AGA03915.1     14
 α-glucosidase 3.2.1.20 Bacillus sp. DG0303 AAF71997.1 Q9L872   29
 oligo-α-1,6-glucosidase 3.2.1.10 Bacillus sp. F5 BAA00534.1 P29093   31
 g-cyclomaltdextrin glucanotransferase (CgtS) 2.4.1.19 Bacillus sp. G-825-6 BAE87038.1 Q25CB6   2
 β-cyclodextrin glucanotransferase 2.4.1.19 Bacillus sp. G1-2004 AAV38118.2 Q5U9V9   2
 maltohexaose-forming amylase (G6-amylase) 3.2.1.98 Bacillus sp. H-167 CAA39096.1 Q45574   19
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. I-5 AAR32682.1 Q6S3E3   2
 maltopentaose-producing α-amylase (Amy204) 3.2.1.- Bacillus sp. JAMB-204 BAF03567.1 Q0KKZ7   5
 β-cyclodextrin glucanotransferase 2.4.1.19 Bacillus sp. KC201 BAA02380.1
2009322A
Q59239   2
 α-amylase (PRK09441) (fragment) 3.2.1.1 Bacillus sp. KR-8104 AAW29920.1 Q5MB94   5
 α-amylase (Kra2;PKA) 3.2.1.1 Bacillus sp. KR8104 ACD93218.3
ACD93218.2
  3DC0[A] 28
 alkaline α-amylase (AmyK) 3.2.1.1 Bacillus sp. KSM-1378 AAR68734.1
AAN99906.1
AAR63625.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
ACS10038.1
ACW26917.1
BAA32431.1
CAD35985.1
CAD38135.1
CAV31709.1
O82839 2DIE[A] 5
 alkaline amylase-pullulanase 3.2.1.1
3.2.1.41
Bacillus sp. KSM-1378 AAS36537.1
AAS36538.1
BAA11332.1
P70983   41
 alkaline amylase-pullulanase 3.2.1.1
3.2.1.41
Bacillus sp. KSM-1378 AAS36537.1
AAS36539.1
BAA11332.1
P70983   12
 neopullulanase 3.2.1.135 Bacillus sp. KSM-1876 BAA02521.1 Q57482   20
 α-amylase (AmyK38) 3.2.1.1 Bacillus sp. KSM-K38 CAC39917.1
AAN18958.1
AAV01046.1
ABA16539.1
ABI05896.1
ABY03537.1
ACS10039.1
BAB71820.1
CAD35986.1
CAD38133.1
CAJ00040.1
CAJ28078.1
Q93I48 1UD2[A]
1UD3[A]
1UD4[A]
1UD5[A]
1UD6[A]
1UD8[A]
5
 α-amylase 3.2.1.1 Bacillus sp. MK 716 AAB18785.1 P71034   5
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Bacillus sp. NR5 UPM ADY17981.1     2
 α-glucosidase 3.2.1.20 Bacillus sp. SAM1606 CAA54266.1
CBF79159.1
Q45517   31
 α-amylase (Amy) 3.2.1.1 Bacillus sp. TS-23 AAA63900.1 Q59222   5
 β-cyclodextrin glucanotransferase 2.4.1.19 Bacillus sp. TS1-1 AAV38117.1 Q5U9W0   2
 maltogenic amylase (MAUS149) 3.2.1.133 Bacillus sp. US149 CAL64396.1 A7DWA8   20
 α-amylase (BMW-amylase) 3.2.1.1 Bacillus sp. WHO ABU54057.1 A7U965   36
 maltogenic α-amylase 3.2.1.133 Bacillus sp. WPD616 AAX85453.1 Q52PU5   20
 α-amylase (AmyF) 3.2.1.1 Bacillus sp. WS06 AAX84031.1
CAZ78870.1
Q4Z8Q4   36
 amylase-pullulanase (AapT) 3.2.1.1
3.2.1.41
Bacillus sp. XAL601 BAA05832.1 Q45643   39
 α-amylase (Amy;BYXA) 3.2.1.1 Bacillus sp. YX / YX-1 ABW87262.1 A9YDD9   5
 oligosaccharide-producing multifunctional amylase (OPMA-N) 3.2.1.135 Bacillus sp. ZW2531-1 ABY71030.1 B0FZ47   20
 oligosaccharide-producing multifunctional amylase (OPMA) 2.4.1.- Bacillus sp. ZW2531-1 ACE77085.1 B3VDZ7   31
 α-amylase 3.2.1.1 Bacillus subtilis 233 AAZ30064.1 Q45N23   28
 α-amylase 3.2.1.1 Bacillus subtilis 2633 CAA30643.1 Q45520 1BAG[A]
1UA7[A]
28
 α-amylase (Amy28) 3.2.1.1 Bacillus subtilis A28 AFQ31615.1     28
 α-amylase (AmyE) 3.2.1.1 Bacillus subtilis BF7658 ACK37366.1 B8Y1H0   28
 α-amylase (Amy7C;Amy7D) (fragment) 3.2.1.1 Bacillus subtilis CN7 AFC90847.1     28
 α-amylase 3.2.1.1 Bacillus subtilis HA401 AAT01440.1 Q6PMJ3   28
 oligo-α-1,6-glucosidase 3.2.1.10 Bacillus subtilis HB002 AAG23399.1
CBF79210.1
Q9F4G4   31
 α-amylase (AmyE) 3.2.1.1 Bacillus subtilis KCC103 ABW34932.1 A8VWC5   28
 α-amylase 3.2.1.1 Bacillus subtilis natto IAM1212 AAA22194.1 Q45516   28
 α-amylase (AmyE;AmyA;BSU03040) 3.2.1.1 Bacillus subtilis subsp. subtilis str. 168 BAA08938.1
AIY91586.1
AQR80240.1
AQR84453.1
CAA23437.1
CAA26086.1
CAB12098.1
CAB12098.2
NP_388186.1
P00691   28
 α-1,6-glucosidase (MalL;YvdL;BSU34560) 3.2.1.10 Bacillus subtilis subsp. subtilis str. 168 CAB15461.1
AIY94772.1
AQR83490.1
AQR87705.1
CAB08041.1
NP_391336.1
O06994 4M56[A,B]
4M8U[A]
4MAZ[A]
4MB1[A]
31
 branching-enzyme (GlgB;GBE;BSU30980) 2.4.1.18 Bacillus subtilis subsp. subtilis str. 168 AAC00214.1
AIY94419.1
AQR83124.1
AQR87339.1
CAA81040.1
CAB15076.1
NP_390976.1
P39118   9
 maltogenic α-amylase / neopullulanase (YvdF;BSU34620) 3.2.1.133 Bacillus subtilis subsp. subtilis str. 168 CAB08035.1
AIY94778.1
AQR83496.1
AQR87711.1
CAB15467.1
NP_391342.1
O06988   20
 pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930) 2.4.1.25
3.2.1.33
3.2.1.41
Bacillus subtilis subsp. subtilis str. 168 AAC00283.1
ACH14984.1
AIY94319.1
AQR83021.1
AQR87236.1
CAB14971.1
CAB14971.2
NP_390871.1
C0SPA0
O34587
2E8Y[A,B]
2E8Z[A,B]
2E9B[A,B]
14
 trehalose-6-phosphate hydrolase (TreA;BSU07810) 3.2.1.93 Bacillus subtilis subsp. subtilis str. 168 CAB12610.1
AIY92070.1
AQR80734.1
AQR84947.1
CAA56495.1
CAA91015.1
NP_388662.1
P39795   29
 α-amylase (Amy) 3.2.1.1 Bacillus subtilis SUH4-2 AAF14358.1
ABW75769.1
Q9R9H7   28
 maltogenic amylase (BbmA) 3.2.1.133 Bacillus subtilis SUH4-2 AAF23874.1 Q9R9H8   20
 α-amylase 3.2.1.1 Bacillus subtilis X-23 BAA31528.1 O82953   28
 maltogenic amylase (BTMA) 3.2.1.133
3.2.1.54
Bacillus thermoalkalophilus ET2 AAT94159.1 Q68KL3   20
 neopullulanase (SusA;BT3704;BT_3704) 3.2.1.135 Bacteroides thetaiotaomicron VPI-5482 AAO78809.1
AAC44670.1
CAZ78747.1
CAZ78867.1
NP_812615.1
P71093
Q8A1G0
 
 α-amylase / neopullulanase (SusG;BT3698;BT_3698) (outer membrane) 3.2.1.1
3.2.1.135
3.2.1.54
Bacteroides thetaiotaomicron VPI-5482 AAO78803.1
AAB42174.1
CAZ78746.1
NP_812609.1
Q45772
Q8A1G3
3K8K[A,B]
3K8M[A,B]
 α-amylase / neopullulanase (SusG;BT3698;BT_3698) (outer membrane) 3.2.1.1
3.2.1.135
3.2.1.54
Bacteroides thetaiotaomicron VPI-5482 AAO78803.1
AAB42174.1
CAZ78746.1
NP_812609.1
Q45772
Q8A1G3
3K8K[A,B]
3K8M[A,B]
 pullulanase (PulI;BT1663;BT_1663) 3.2.1.41 Bacteroides thetaiotaomicron VPI-5482 AAO76770.1
AAC44685.1
NP_810576.1
P71095
Q8A762
  14
 branching enzyme (GlgB) 2.4.1.18 Bergeriella denitrificans AAF04747.1
AAQ32649.1
AAT15256.1
CAC22097.1
Q9RQI5   9
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