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Glycoside Hydrolase Family 13

Activities in Family[retaining] α-glucoside phosphorylase (EC 2.4.1.-);
[retaining] α-maltosyltransferase (EC 2.4.1.-);
[retaining] α-transglucosidase / α-glucosyltransferase (EC 2.4.1.-);
[retaining] acarviosyltransferase (EC 2.4.1.-);
α-1,6-maltooligosyltransferase (EC 2.4.1.-);
α-glucosyltransferase (EC 2.4.1.-);
Oligosaccharide α-4-glucosyltransferase (EC 2.4.1.161);
[retaining] α-1,4-glucan branching enzyme (EC 2.4.1.18);
[retaining] cyclomaltodextrin glucanotransferase (EC 2.4.1.19);
[retaining] 4-α-glucanotransferase / amylomaltase (EC 2.4.1.25);
[retaining] sucrose 6(F)-phosphate phosphorylase (EC 2.4.1.329);
Glucosylglycerate phosphorylase (EC 2.4.1.352);
[retaining] glucosylglycerol phosphorylase (EC 2.4.1.359);
[retaining] amylosucrase (EC 2.4.1.4);
[retaining] sucrose phosphorylase (EC 2.4.1.7);
α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16);
[retaining] cyclic α-1,6-maltosyl-maltose hydrolase (EC 3.2.1.-);
[retaining] maltopentaose-producing α-amylase (EC 3.2.1.-);
Palatinase (EC 3.2.1.-);
[retaining] α-amylase (EC 3.2.1.1);
[retaining] oligo-α-1,6-glucosidase (EC 3.2.1.10);
[retaining] maltotriose-producing α-amylase (EC 3.2.1.116);
[retaining] maltogenic α-amylase (EC 3.2.1.133);
[retaining] neopullulanase (EC 3.2.1.135);
[retaining] maltooligosyltrehalose trehalohydrolase (EC 3.2.1.141);
α-glucosidase (EC 3.2.1.20);
α,α-trehalase (EC 3.2.1.28);
[retaining] pullulanase (EC 3.2.1.41);
[retaining] sucrose α-glucosidase (EC 3.2.1.48);
[retaining] cyclomaltodextrinase (EC 3.2.1.54);
[retaining] maltotetraose-producing α-amylase (EC 3.2.1.60);
Isoamylase (EC 3.2.1.68);
Glucodextranase (EC 3.2.1.70);
[retaining] a,α-trehalose-6-phosphate hydrolase (EC 3.2.1.93);
[retaining] maltohexaose-producing α-amylase (EC 3.2.1.98);
[retaining] isomaltulose synthase / sucrose isomerase / sucrose glucosylmutase (EC 5.4.99.11);
Malto-oligosyltrehalose synthase (EC 5.4.99.15);
Trehalose synthase / maltose glucosylmutase (EC 5.4.99.16);
Mechanism Retaining a
ClanGH-H
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (176406); Uniprot accession (956); PDB accession (656); 3D entries (161); cryst (2)
Summary
Download GH13 (179264) Taxonomic display Structure (161 - 2 cryst) Characterized (815)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 a/β-cyclodextrin glucanotransferase (CgtA) 2.4.1.19 pubmed
 Bacillus licheniformis CAA33763.1 P14014   2
 maltogenic α-amylase (BLMA) (frameshift) 3.2.1.133 pubmed
 Bacillus licheniformis CAA47612.1 Q04977   20
 α-amylase (AmyL) 3.2.1.1   Bacillus licheniformis 44MB82/G AGR34077.1 Q208A7   5
 α-amylase (AmyA;AmyL;AmyS;BLA) 3.2.1.1 pubmed
 Bacillus licheniformis 584 / ATCC 27811 AAA22226.1
AAA22193.1
AAA22240.1
AAN99913.1
AAO26743.1
AAT46561.1
ACM17155.1
CAA01355.1
CAA03075.1
CAA26981.1
CAC16483.1
CAC88550.1
CAD19034.1
CAD20677.1
CAD26701.1
CAD26706.1
CAD88111.1
CAV31708.1		 P06278
Q208A7
Q6GWE2		 1BLI[A]
1BPL[A,B]
1OB0[A]
1VJS[A] 		5
 amylase 3.2.1.1   Bacillus licheniformis ATCC 9945a AEM05860.1 I3P686 6TOY[A]
6TOZ[A]
6TP0[A]
6TP1[A]
6TP2[A] 		5
 α-amylase (AmyL) 3.2.1.1   Bacillus licheniformis B0204 ABD66592.1 Q208A7   5
 α-amylase (thermostable) 3.2.1.1   Bacillus licheniformis CICC 10181 ACN88150.1 C0LZX2   5
 trehalose-6-P hydrolase (TreA;BlTreA;BLi00797;BL03069) 3.2.1.93   Bacillus licheniformis DSM 13 = ATCC 14580 AAU39732.1
AAU22384.1
CBF79089.1		 Q65MI2 5BRP[A,B,C,D]
5BRQ[A,B,C,D] 		29
 α-amylase (Amy;BLi00656;BL00499) 3.2.1.1   Bacillus licheniformis DSM 13 = ATCC 14580 AAU39594.1
AAU22245.1		 Q65MX0   5
 α-amylase (AmyB) 3.2.1.1   Bacillus licheniformis MD1 CAH10338.1 P06278
Q6GWE2		   5
 α-amylase 3.2.1.1   Bacillus licheniformis NCIB8061 AAE12384.1     5
 α-amylase (AmyN) 3.2.1.1   Bacillus licheniformis NH1 ABL75259.1 A1YR25   5
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus ohbensis C-1400 BAA14289.2
I39805		 P27036   2
 α-amylase (AmyL;BaLi_c07450) 3.2.1.1 pubmed
 Bacillus paralicheniformis ATCC 9945a AGN35141.1     5
 cyclomaltodextrinase (CDI5;BspCMD) 3.2.1.54 pubmed
 Bacillus sp. (in: firmicutes) AAA92925.1 Q59226 1EA9[C] 20
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus sp. (in: firmicutes) 17-1 AAA22310.1 P30921   2
 α-amylase (BAA) 3.2.1.1 pubmed
 Bacillus sp. (in: firmicutes) 195 BAA22082.1 O24781   32
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus sp. (in: firmicutes) 38-2 AAA22309.1
BAA00077.1		 P09121   2
 cyclodextrinase 3.2.1.54 pubmed
 Bacillus sp. (in: firmicutes) A2-5A BAA31576.1 O82982   20
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus sp. (in: firmicutes) A2-5A BAA31539.1 O82984   2
 α-glucosidase with high transglucosylation activity (BspAG13_31A) 3.2.1.20 pubmed
 Bacillus sp. (in: firmicutes) AHU2216 BBB94188.1 A0A2Z5WH92 5ZCB[A]
5ZCC[A]
5ZCD[A]
5ZCE[A]
8IBK[A]
8IDS[A] 		31
 maltohexaose-forming amylase (G6-amylase) 3.2.1.98 pubmed
 Bacillus sp. (in: firmicutes) H-167 CAA39096.1 Q45574   19
 alkaline α-amylase (AmyK) 3.2.1.1   Bacillus sp. (in: firmicutes) KSM-1378 AAR68734.1
AAN99906.1
AAR63625.1
AAV01045.1
ABE15847.1
ABI05897.1
ABY03536.1
ACS10038.1
ACW26917.1
BAA32431.1
CAD35985.1
CAD38135.1
CAV31709.1		 O82839 2DIE[A] 5
 alkaline amylase-pullulanase 3.2.1.1 pubmed
 Bacillus sp. (in: firmicutes) KSM-1378 AAS36537.1
AAS36538.1
BAA11332.1		 P70983   41
 alkaline amylase-pullulanase 3.2.1.41 pubmed
 Bacillus sp. (in: firmicutes) KSM-1378 AAS36537.1
AAS36539.1
BAA11332.1		 P70983   12
 α-glucosidase 3.2.1.20 pubmed
 Bacillus sp. (in: firmicutes) SAM1606 CAA54266.1
CBF79159.1		 Q45517   31
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus sp. 1-1   P31746   2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 pubmed
 Bacillus sp. 1011 AAA22308.1 P05618 1D7F[A]
1DED[A]
1I75[A]
1PAM[A]
1UKQ[A,B]
1UKS[A,B]
1UKT[A,B]
1V3J[A,B]
1V3K[A,B]
1V3L[A,B]
1V3M[A,B] 		2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19   Bacillus sp. 20RF AEL33336.1 G0ZI10   2
 maltohexaose-forming amylase 3.2.1.98 pubmed
 Bacillus sp. 707 AAA22231.1
AAE59728.1
AAE62350.1
AAP02212.1
AAR63620.1
AAS26292.1
AAY06019.1
AAY72174.1
ACK07346.1
ACP75071.1
ACS10032.1
ACW26905.1		 P19571 1WP6[A]
1WPC[A]
2D3L[A]
2D3N[A] 		5
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19   Bacillus sp. 8SB AEL33337.1 G0ZI11   2
 α-amylase (AmyL) 3.2.1.1 pubmed
 Bacillus sp. AAH-31 BAN63122.1 S6BGD1   20
 isoamylase / pullulanase type I (BsIam; PulA1) 3.2.1.41
3.2.1.68		 pubmed
pubmed
 Bacillus sp. CICIM 263 AGA03915.1 L0C9D3   14
 α-glucosidase 3.2.1.20   Bacillus sp. DG0303 AAF71997.1 Q9L872   29
 oligo-α-1,6-glucosidase 3.2.1.10 doi
 Bacillus sp. F5 BAA00534.1 P29093   31
 g-cyclomaltdextrin glucanotransferase (CgtS) 2.4.1.19   Bacillus sp. G-825-6 BAE87038.1 Q25CB6   2
 β-cyclodextrin glucanotransferase 2.4.1.19   Bacillus sp. G1-2004 AAV38118.2 Q5U9V9   2
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19   Bacillus sp. I-5 AAR32682.1 Q6S3E3   2
 maltopentaose-producing α-amylase (Amy204) 3.2.1.-   Bacillus sp. JAMB-204 BAF03567.1 Q0KKZ7   5
 β-cyclodextrin glucanotransferase 2.4.1.19 doi
 Bacillus sp. KC201 BAA02380.1
2009322A		 Q59239   2
 α-amylase (PRK09441) (fragment) 3.2.1.1   Bacillus sp. KR-8104 AAW29920.1 Q5MB94   5
 α-amylase (Kra2;PKA) 3.2.1.1   Bacillus sp. KR8104 ACD93218.3
ACD93218.2		 B3GQD0 3DC0[A] 28
 neopullulanase 3.2.1.135 pubmed
 Bacillus sp. KSM-1876 BAA02521.1 Q57482   20
 α-amylase (AmyK38) 3.2.1.1 doi
 Bacillus sp. KSM-K38 CAC39917.1
AAN18958.1
AAV01046.1
ABA16539.1
ABI05896.1
ABY03537.1
ACS10039.1
BAB71820.1
CAD35986.1
CAD38133.1
CAJ00040.1
CAJ28078.1		 Q93I48 1UD2[A]
1UD3[A]
1UD4[A]
1UD5[A]
1UD6[A]
1UD8[A] 		5
 β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19   Bacillus sp. NR5 UPM ADY17981.1 F1DPT1   2
 α-amylase (Amy) 3.2.1.1 pubmed
 Bacillus sp. TS-23 AAA63900.1 Q59222   5
 β-cyclodextrin glucanotransferase 2.4.1.19   Bacillus sp. TS1-1 AAV38117.1 Q5U9W0   2
 maltogenic amylase (MAUS149) 3.2.1.133   Bacillus sp. US149 CAL64396.1 A7DWA8   20
 α-amylase (BMW-amylase) 3.2.1.1   Bacillus sp. WHO ABU54057.1 A7U965   36
 maltogenic α-amylase 3.2.1.133 doi
 Bacillus sp. WPD616 AAX85453.1 Q52PU5   20
 α-amylase (AmyF) 3.2.1.1   Bacillus sp. WS06 AAX84031.1
CAZ78870.1		 Q4Z8Q4   36
 amylase-pullulanase (AapT) 3.2.1.1
3.2.1.41		 pubmed
pubmed
 Bacillus sp. XAL601 BAA05832.1 Q45643   39
 α-amylase (Amy;BYXA) 3.2.1.1   Bacillus sp. YX / YX-1 ABW87262.1 A9YDD9   5
 oligosaccharide-producing multifunctional amylase (OPMA-N) 3.2.1.135   Bacillus sp. ZW2531-1 ABY71030.1 B0FZ47   20
 α-amylase 3.2.1.1 pubmed
 Bacillus subtilis 2633 CAA30643.1 Q45520 1BAG[A]
1UA7[A] 		28
 α-amylase (Amy28) 3.2.1.1   Bacillus subtilis A28 AFQ31615.1 J9QPI9   28
 α-amylase (AmyE) 3.2.1.1   Bacillus subtilis BF7658 ACK37366.1 B8Y1H0   28
 α-amylase (Amy7C;Amy7D) (fragment) 3.2.1.1 pubmed
 Bacillus subtilis CN7 AFC90847.1 H9B4I9   28
 α-amylase 3.2.1.1   Bacillus subtilis HA401 AAT01440.1 Q6PMJ3   28
 oligo-α-1,6-glucosidase 3.2.1.10   Bacillus subtilis HB002 AAG23399.1
CBF79210.1		 Q9F4G4   31
 α-amylase (AmyE) 3.2.1.1   Bacillus subtilis KCC103 ABW34932.1 A8VWC5   28
 α-amylase 3.2.1.1   Bacillus subtilis natto IAM1212 AAA22194.1 Q45516   28
 α-amylase (AmyE;AmyA;BSU03040) 3.2.1.1 pubmed
 Bacillus subtilis subsp. subtilis str. 168 BAA08938.1
AIY91586.1
AQR80240.1
AQR84453.1
CAA23437.1
CAA26086.1
CAB12098.1
CAB12098.2
NP_388186.1		 P00691   28
 α-1,6-glucosidase (MalL;YvdL;BSU34560) 3.2.1.10 pubmed
 Bacillus subtilis subsp. subtilis str. 168 CAB15461.1
AIY94772.1
AQR83490.1
AQR87705.1
CAB08041.1
NP_391336.1		 A0A6M4JPF4
O06994		 4M56[A,B]
4M8U[A]
4MAZ[A]
4MB1[A]
5WCZ[A,B]
7LV6[A] 		31
 maltogenic α-amylase / neopullulanase (YvdF;BSU34620) 3.2.1.133 pubmed
 Bacillus subtilis subsp. subtilis str. 168 CAB08035.1
AIY94778.1
AQR83496.1
AQR87711.1
CAB15467.1
NP_391342.1		 A0A6M4JPH5
O06988		   20
 pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930) 3.2.1.41 pubmed
 Bacillus subtilis subsp. subtilis str. 168 AAC00283.1
ACH14984.1
AIY94319.1
AQR83021.1
AQR87236.1
CAB14971.1
CAB14971.2
NP_390871.1		 A0A6M4JN50
C0SPA0
O34587		 2E8Y[A,B]
2E8Z[A,B]
2E9B[A,B] 		14
 trehalose-6-phosphate hydrolase (TreA;BSU07810) 3.2.1.93 pubmed
 Bacillus subtilis subsp. subtilis str. 168 CAB12610.1
AIY92070.1
AQR80734.1
AQR84947.1
CAA56495.1
CAA91015.1
NP_388662.1		 A0A6M3Z8J1
P39795		   29
 α-amylase (Amy) 3.2.1.1   Bacillus subtilis SUH4-2 AAF14358.1
ABW75769.1		 A8W7J1
Q9R9H7		   28
 maltogenic amylase (BbmA) 3.2.1.133   Bacillus subtilis SUH4-2 AAF23874.1 Q9R9H8   20
 α-amylase 3.2.1.1 pubmed
 Bacillus subtilis X-23 BAA31528.1 O82953   28
 α-amylase 3.2.1.1 pubmed
 Bacillus subtilis XL8 QBC17803.1 A0A411J1E3   28
 maltogenic amylase (BTMA) 3.2.1.133 doi
 Bacillus thermoalkalophilus ET2 AAT94159.1 Q68KL3   20
 α-glucosidase activity on pNP-α-D-maltopyranoside (BcellWH2_05530;BACWH2_5225; TvaII) 3.2.1.20 pubmed
 Bacteroides cellulosilyticus WH2 ALJ62728.1
WP_029428030.1		 A0A0P0GJC2   38
 α-amylase / neopullulanase (BoSusG; BoGH13A; Bovatus_03803) 3.2.1.1
3.2.1.135		 pubmed
 Bacteroides ovatus ATCC 8483 ALJ48408.1   8DGE[A,B,C,D]
8DL1[A,B,C,D]
8DL2[A,B,C,D] 		47
 neopullulanase (SusA; BT3704; BT_3704) 3.2.1.135 pubmed
 Bacteroides thetaiotaomicron VPI-5482 AAO78809.1
AAC44670.1
CAZ78747.1
CAZ78867.1
NP_812615.1		 P71093
Q8A1G0		   46
 α-amylase / neopullulanase (SusG; BtSusG; BT3698; BT_3698) (outer membrane) 3.2.1.1
3.2.1.135		 pubmed
pubmed
pubmed
pubmed
 Bacteroides thetaiotaomicron VPI-5482 AAO78803.1
AAB42174.1
CAZ78746.1
NP_812609.1		 Q45772
Q8A1G3		 3K8K[A,B]
3K8L[A,B]
3K8M[A,B]
6BS6[A,B] 		36
 α-amylase / neopullulanase (SusG; BtSusG; BT3698; BT_3698) (outer membrane) 3.2.1.1
3.2.1.135		 pubmed
pubmed
pubmed
pubmed
 Bacteroides thetaiotaomicron VPI-5482 AAO78803.1
AAB42174.1
CAZ78746.1
NP_812609.1		 Q45772
Q8A1G3		 3K8K[A,B]
3K8L[A,B]
3K8M[A,B]
6BS6[A,B] 		36
 pullulanase (PulI; BT1663; BT_1663) 3.2.1.41 pubmed
 Bacteroides thetaiotaomicron VPI-5482 AAO76770.1
AAC44685.1
NP_810576.1		 P71095
Q8A762		   14
 branching enzyme (GlgB) 2.4.1.18   Bergeriella denitrificans AAF04747.1
AAQ32649.1
AAT15256.1
CAC22097.1		 Q9RQI5   9
 α-glucosidase (CG400_06090) 3.2.1.20 pubmed
 Bifidobacteriaceae bacterium NR017 RFT33048.1 A0A3E2CLU2   44
 α-glucosidase (AglB;AglA;BAD_0452) 3.2.1.20 pubmed
 Bifidobacterium adolescentis ATCC 15703 AAL05573.1
BAF39233.1
CBF79196.1		 A1A0K0
Q93CA0		   30
 sucrose phosphorylase (SucP;SP;BaSP) 2.4.1.7   Bifidobacterium adolescentis DSM 20083 AAO33821.1 A0ZZH6
Q84HQ2		 1R7A[A,B]
2GDU[A,B]
2GDV[A,B]
5C8B[B]
5M9X[B]
5MAN[B]
5MB2[B]
6FME[A,B] 		18
 oligo α-glucosidase (AglA) 3.2.1.10 pubmed
 Bifidobacterium adolescentis DSM 20083 AAK27723.1 Q9AF93   31
 α-amylase (AmyB) 3.2.1.1   Bifidobacterium adolescentis INT-57 AAP69820.1 Q5JB42   28
 α-1,6-glucosidase 1 (Agl1; Bbr_1855) 3.2.1.10
3.2.1.20		   Bifidobacterium breve UCC2003 ACM89182.1
ABE96517.1		 C0JP82   31
 α-1,6-glucosidase 2 (Agl2; Bbr_0559) 3.2.1.10
3.2.1.20		   Bifidobacterium breve UCC2003 ACM89183.1
ABE95255.1		 C0JP83   31
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.41   Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1		 F9Y041
Q0R5Z4		   14
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.1   Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1		 F9Y041
Q0R5Z4		   32
 sucrose phosphorylase (Spl;BL0536) 2.4.1.7   Bifidobacterium longum NCC2705 AAN24362.1
NP_695726.1		 Q8G6U7   18
 sucrose phosphorylase (SplP) 2.4.1.7   Bifidobacterium longum SJ32 AAO84039.1 Q84BY1   18
 sucrose phosphorylase (Spl) 2.4.1.7   Bifidobacterium longum subsp. longum JCM 1217 BAF62433.1 A5A8M5   18
 α-glucosidase 2 (Agl_2; BlAglA2; BLGA_19140) 3.2.1.10
3.2.1.20		 URL
URL
 Bifidobacterium longum subsp. longum JCM 7052 BBV24503.1     31
 α-glucosidase (Agl_1; BlAglA1; BLGA_18600) 3.2.1.10
3.2.1.20		 URL
URL
 Bifidobacterium longum subsp. longum JCM 7052 BBV24449.1     31
 α-glucosidase C (GluC; BpGluC; AW18_01930) 3.2.1.20 pubmed
 Bifidobacterium pseudocatenulatum IPLA36007 KEF29304.1     31
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116		 pubmed
pubmed
 Brachybacterium sp. LB25 BAE94180.1 Q1JUA3   32
 β-cyclodextrin glycosyltransferase (Cgt) 2.4.1.19 pubmed
 Brevibacillus brevis CD162 AAB65420.1 O30565   2
 maltooligosyltrehalose synthase (TreY;BvMts) 5.4.99.15 pubmed
 Brevibacterium helvolum AAB95368.1 O52519   26
 maltooligosyltrehalose trehalohydrolase (TreZ;BvMth) 3.2.1.141 pubmed
 Brevibacterium helvolum AAB95369.1 O52520   10
 sucrose isomerase / trehalulose synthase (MutB) 5.4.99.11 pubmed
 Burkholderia ubonensis subsp. mesacidophila MX-45 ABC33903.1
AAY69574.1
ABT00107.1
ACO05018.1
CAF32995.1		 M1E1F3
M1E1F6
M1E1F7
Q2PS28		 1ZJA[A,B]
1ZJB[A,B]
2PWD[A,B]
2PWE[A,B]
2PWF[A,B,C,D]
2PWG[A,B]
2PWH[A,B]
4GI6[A,B]
4GI8[A,B]
4GI9[A,B]
4GIA[A,B]
4GIN[A]
4GO8[A,B]
4GO9[A,B]
4H2C[A]
4H7V[A,B]
4H8H[A,B]
4H8U[A,B]
4H8V[A,B]
4HA1[A,B] 		31
 palatinase / trehalulose hydrolase (MutA) 3.2.1.10
3.2.1.-		   Burkholderia ubonensis subsp. mesacidophila MX-45 CAA02858.1
AAE36430.1
AAY69575.1
ACO05017.1
ACO05017.2		 C1K2X6   31
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