Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54252); Uniprot accession (7858); PDB accession (465); 3D entries (110); cryst (2)
All (49476) Archaea (367) Bacteria (45982) Eukaryota (2997) unclassified (130) Structure (110 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Eukaryota
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 branching enzyme IIb (Sbe1;SBEIIb) 2.4.1.18 Zea mays AAA18571.1
AAB33385.1
AAC33764.1
AAR75247.1
AAU99022.1
ABC04875.1
ABE12596.1
ABO25741.1
ABX94246.1
ACG45498.1
ACM95319.1
ACN30754.1
O81387
Q08047
  8
 isoamylase (Isa1;Su1;Su-1;Sugary1;Sugary-1) 3.2.1.68 Zea mays ACG43008.1
AAA91298.1
AAB97167.1
AAE25386.1
AAE25387.1
AAR75249.1
ABE20876.1
B6U0X5
O22637
Q41742
  11
 pullulanase (Zpu1) 3.2.1.41 Zea mays AAD11599.1
AAR75242.1
AAV99892.1
AKO22325.1
CAB69602.1
O81638   13
unclassified
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 Npu193A 3.2.1.- uncultured microorganism ABS59068.1 A7LI67   20
 α-amylase (BD5063) 3.2.1.1 uncultured organism AAM48114.1
ACH26097.1
Q8JZK3   7
 α-amylase 3.2.1.- uncultured organism ADD61603.1 D9ZE49  
 amylase 3.2.1.- uncultured organism ADD61706.1 D9ZEF2   11
 α-amylase 3.2.1.- uncultured organism ADD61616.1 D9ZE62  
 α-amylase 3.2.1.- uncultured organism ADD61669.1 D9ZEB5   14
 amylase 3.2.1.- uncultured organism ADD61743.1 D9ZEH9   14
 ORF 3.2.1.1 uncultured organism AGU10738.1     6
 cyclomaltodextrinase (RA.04;RA.04-1;AmyA) 3.2.1.54 unidentified microorganism CAJ19153.1 Q2YI50  
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