Activities in Family | [retaining] α-glucoside phosphorylase (EC 2.4.1.-); [retaining] α-maltosyltransferase (EC 2.4.1.-); [retaining] α-transglucosidase / α-glucosyltransferase (EC 2.4.1.-); [retaining] acarviosyltransferase (EC 2.4.1.-); α-1,6-maltooligosyltransferase (EC 2.4.1.-); α-glucosyltransferase (EC 2.4.1.-); Oligosaccharide α-4-glucosyltransferase (EC 2.4.1.161); [retaining] α-1,4-glucan branching enzyme (EC 2.4.1.18); [retaining] cyclomaltodextrin glucanotransferase (EC 2.4.1.19); [retaining] 4-α-glucanotransferase / amylomaltase (EC 2.4.1.25); [retaining] sucrose 6(F)-phosphate phosphorylase (EC 2.4.1.329); Glucosylglycerate phosphorylase (EC 2.4.1.352); [retaining] glucosylglycerol phosphorylase (EC 2.4.1.359); [retaining] amylosucrase (EC 2.4.1.4); [retaining] sucrose phosphorylase (EC 2.4.1.7); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); [retaining] cyclic α-1,6-maltosyl-maltose hydrolase (EC 3.2.1.-); [retaining] maltopentaose-producing α-amylase (EC 3.2.1.-); Palatinase (EC 3.2.1.-); [retaining] α-amylase (EC 3.2.1.1); [retaining] oligo-α-1,6-glucosidase (EC 3.2.1.10); [retaining] maltotriose-producing α-amylase (EC 3.2.1.116); [retaining] maltogenic α-amylase (EC 3.2.1.133); [retaining] neopullulanase (EC 3.2.1.135); [retaining] maltooligosyltrehalose trehalohydrolase (EC 3.2.1.141); α-glucosidase (EC 3.2.1.20); α,α-trehalase (EC 3.2.1.28); [retaining] pullulanase (EC 3.2.1.41); [retaining] sucrose α-glucosidase (EC 3.2.1.48); [retaining] cyclomaltodextrinase (EC 3.2.1.54); [retaining] maltotetraose-producing α-amylase (EC 3.2.1.60); Isoamylase (EC 3.2.1.68); Glucodextranase (EC 3.2.1.70); [retaining] a,α-trehalose-6-phosphate hydrolase (EC 3.2.1.93); [retaining] maltohexaose-producing α-amylase (EC 3.2.1.98); [retaining] isomaltulose synthase / sucrose isomerase / sucrose glucosylmutase (EC 5.4.99.11); Malto-oligosyltrehalose synthase (EC 5.4.99.15); Trehalose synthase / maltose glucosylmutase (EC 5.4.99.16); |
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Mechanism | Retaining a |
Clan | GH-H |
3D Structure Status | ( β / α ) 8 barrel |
Catalytic Nucleophile/Base | Asp (experimental) |
Catalytic Proton Donor | Glu (experimental) |
Note | New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431) |
External resources | CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS; |
Commercial Enzyme Provider(s) | MEGAZYME; PROZOMIX; |
Statistics | GenBank accession (176406); Uniprot accession (956); PDB accession (656); 3D entries (161); cryst (2) |
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Eukaryota | |||||||
Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf |
branching enzyme I | 2.4.1.18 | Zea mays | AAA82735.1 AAC36471.1 AAO20100.1 AAR75248.1 AAU99031.1 ABA16989.1 ABE12598.1 ABJ42248.1 ABQ15209.1 ABX94247.1 ACG32270.1 ACM95328.1 ACN28680.1 ALN66063.1 BAA01854.1 |
A5HJZ8 Q41740 Q7DNA5 Q84XW7 |
8 | ||
branching enzyme IIa | 2.4.1.18 | Zea mays | AAB67316.1 AAR75246.1 AAU99021.1 ABJ42249.1 ACM95318.1 |
O24421 | 8 | ||
branching enzyme IIb (Sbe1;SBEIIb) | 2.4.1.18 | Zea mays | AAA18571.1 AAB33385.1 AAC33764.1 AAR75247.1 AAU99022.1 ABC04875.1 ABE12596.1 ABO25741.1 ABX94246.1 ACG45498.1 ACM95319.1 ACN30754.1 |
A4GUI1 C0P9A9 O81387 Q08047 |
8 | ||
isoamylase (Isa1;Su1;Su-1;Sugary1;Sugary-1) | 3.2.1.68 | Zea mays | ACG43008.1 AAA91298.1 AAB97167.1 AAE25386.1 AAE25387.1 AAR75249.1 ABE20876.1 |
B6U0X5 O22637 Q41742 |
11 | ||
pullulanase (Zpu1) | 3.2.1.41 | Zea mays | AAD11599.1 AAR75242.1 AAV99892.1 AKO22325.1 CAB69602.1 |
O81638 | 13 | ||
unclassified | |||||||
Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf |
pullulanase (Npu193A) | 3.2.1.41 | pubmed |
uncultured microorganism | ABS59068.1 | A7LI67 | 20 | |
α-amylase (BD5063) | 3.2.1.1 | uncultured organism | AAM48114.1 ACH26097.1 |
Q8JZK3 | 7 | ||
α-amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61603.1 | D9ZE49 | ||
amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61706.1 | D9ZEF2 | 11 | |
α-amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61616.1 | D9ZE62 | ||
α-amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61669.1 | D9ZEB5 | 14 | |
α-amylase | 3.2.1.1 | pubmed |
uncultured organism | ADD61689.1 | D9ZED5 | 5 | |
amylase | 3.2.1.20 | pubmed |
uncultured organism | ADD61743.1 | D9ZEH9 | 14 | |
ORF | 3.2.1.1 | uncultured organism | AGU10738.1 | T1W7I3 | 6 | ||
cyclomaltodextrinase (RA.04;RA.04-1;AmyA) | 3.2.1.54 | pubmed |
unidentified microorganism | CAJ19153.1 | Q2YI50 | 46 | |
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