Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (55939); Uniprot accession (7858); PDB accession (467); 3D entries (111); cryst (2)
All (51170) Archaea (370) Bacteria (47636) Eukaryota (3034) unclassified (130) Structure (111 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 branching-enzyme 2.4.1.18 Kitasatospora aureofaciens AAA67437.1 P52980   9
 pullulanase (PulA) 3.2.1.41 Klebsiella aerogenes W70 AAA25124.1
AAR52613.1
P07811   13
 cyclodextrinase (CymH) 3.2.1.54 Klebsiella oxytoca M5A1 CAA60007.1
CAY02962.1
CAZ78869.1
Q48398   20
 maltohexaose-forming α-amylase (AmyG6) 3.2.1.98 Klebsiella pneumoniae BAA88434.1 Q9RHR1   19
 α-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Klebsiella pneumoniae M5a1 AAA25059.1
AAE32693.1
AAP61132.1
AAP61133.1
P08704   2
 trehalose-6-phosphate hydrolase (TreC;KP1_0530) 3.2.1.93 Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044 BAG86620.1
BAH61402.1
C4X1I2   29
 pullulanase A (PulA) 3.2.1.41 Klebsiella pneumoniae UNF5023 CAA36431.1
AAA61976.1
ACH14985.1
P07206 2FGZ[A]
2FH6[A]
2FH8[A]
2FHB[A]
2FHC[A]
2FHF[A]
13
 isomaltulose synthase (PalI) 5.4.99.11 Klebsiella sp. LX3 AAK82938.1
CAF32992.1
CAF32993.1
CBF79192.1
Q8KR84 1M53[A] 31
 sucrose isomerase (PalI) 5.4.99.11 Klebsiella sp. NK33-98-8 AAM96902.1 Q4L2Q1   31
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141 Kocuria rosea QS412 AAY83363.1 Q4JQI8   10
 α-amylase (Amy;KVA) 3.2.1.1 Kocuria varians ATCC 21971 BAJ52728.1     32
 cyclomaltodextrinase (Cda13;Amy80) 3.2.1.54 Laceyella sacchari AAX29990.1
CAY02964.1
CAZ78872.1
Q5BLZ7   20
 sucrose phosphorylase (LaSP;GtfA2;LBA1437) 2.4.1.7 Lactobacillus acidophilus NCFM AAO21868.1
AAV43262.1
ACK31408.1
G1UB42
Q7WWP8
  18
 oligo-α-1,6-glucosidase (LBA0264) 3.2.1.10 Lactobacillus acidophilus NCFM AAV42157.1 Q5FMB7 4AIE[A] 31
 LBA1014 (TreC;La1014) 3.2.1.93 Lactobacillus acidophilus NCFM AAV42863.1
ACH26473.1
Q5FKB1   29
 α-amylase (AmyA) 3.2.1.1 Lactobacillus amylovorus NRRL B-45 AAC45781.1
ABO77965.1
CAA56557.1
A4ULJ3
Q48502
Q79AD6
  28
 maltogenic α-amylase (LgMA;Lgas02001568) 3.2.1.133 Lactobacillus gasseri ATCC 33323 = JCM 1131 ZP_00047084.1 Q046J8   20
 maltogenic α-amylase (Lgma;LGAS_0215) 3.2.1.133 Lactobacillus gasseri ATCC 33323 = JCM 1131 ABJ59624.1 Q046J8   20
 α-amylase (AmyA) 3.2.1.1 Lactobacillus manihotivorans LMG 18010T AAD45245.1
AAC24760.1
O85007
Q9XCV8
  28
 α-amylase 3.2.1.1 Lactobacillus plantarum A6 AAC45780.1 O06915   28
 amylopullulanase (ApuA) 3.2.1.1
3.2.1.41
Lactobacillus plantarum L137 BAF93906.1
BAG67031.1
A9EEQ2   14
 sucrose phosphorylase (ScrP) 2.4.1.7 Lactobacillus reuteri LTH5448 AGK37834.1     18
 sucrose phosphorylase (LmSPase) 2.4.1.7 Leuconostoc mesenteroides ATCC 12291 BAA14344.1 Q59495   18
 sucrose phosphorylase (742sp) 2.4.1.7 Leuconostoc mesenteroides B-742 ABS59292.1 B2BS85   18
 sucrose phosphorylase 2.4.1.7 Leuconostoc mesenteroides NO. 165 2207198A     18
 sucrose phosphorylase (LmSP1) 2.4.1.7 Leuconostoc mesenteroides NRRL B-1149 AAX33736.1 Q14EH6   18
 α-1,6-glucosidase (Lmo0184) 3.2.1.20 Listeria monocytogenes EGD-e NP_463715.1
CAC98399.1
CAZ78714.1
Q8YAE6 5DO8[A,B,C] 31
 cyclodextrinase (CDase) 3.2.1.54 Lysinibacillus sphaericus E-244 CAA44454.1
CAY02969.1
CAZ78898.1
Q08341   20
 trehalose synthase (TreS) 5.4.99.16 Meiothermus ruber CBS-01 ACA35051.1 B1PK99   16
 amylosucrase (MFAS;Mfla_2611) 2.4.1.4 Methylobacillus flagellatus KT ABE50875.1 Q1GY12   4
 α-glucan 1,4-α-maltohexaosidase (AmyB;MaAmyB) 3.2.1.98 Microbacterium aurum B8.A AOF40721.1     42
 α-amylase (AmyA;MaAmyA) 3.2.1.1 Microbacterium aurum B8A AKG25402.1     32
 maltotriose-producing α-amylase (AmyA) 3.2.1.116 Microbulbifer thermotolerans DAU221 AID53183.1
AMX04264.1
    2
 α-amylase 3.2.1.1 Micrococcus sp. 207 CAA39321.1 Q06812   41
 pullulanase type I (AmyA1) 3.2.1.41 mixed culture bacterium AmyA1 AGW21593.1     14
 trehalose synthase (TreS;MsTS;MSMEG_6515) 5.4.99.16 Mycobacterium smegmatis str. MC2 155 ABK71531.1
AFP42769.1
AIU11490.1
A0R6E0 3ZO9[A,B]
3ZOA[A,B]
5JY7[A,B,C,D,E,F,G,H]
16
 α-1,4-glucan: phosphate α-maltosyltransferase (GlgE;KEK_12948) 2.4.99.16 Mycobacterium thermoresistibile ATCC 19527 EHI11807.1   5CGM[A,B]
5CIM[A,B]
5CJ5[A,B]
3
 α-1,4-glucan: phosphate α-maltosyltransferase (GlgE;Rv1327c) 2.4.99.16 Mycobacterium tuberculosis H37Rv CAA98091.2
AFN49234.1
CCP44085.1
  4U33[A,B,C,D,E,F]
4U3C[A,B,C,D,E,F]
3
 branching-enzyme (GlgB;Rv1326c) 2.4.1.18 Mycobacterium tuberculosis H37Rv CAA98090.1
AFN49233.1
CCP44084.1
NP_215842.1
Q10625 3K1D[A] 9
 trehalose synthase (TreS;MtTS;Rv0126;RVBD_0126) 5.4.99.16 Mycobacterium tuberculosis H37Rv CAB09454.1
AFN47974.1
CCP42851.1
NP_214640.1
O07176 4LXF[A,B] 16
 MYPU_6440 3.2.1.41 Mycoplasma pulmonis UAB CTIP CAC13817.1
NP_326475.1
Q98PS5  
 isoamylase 3.2.1.68 Myroides odoratus BAA82695.1 Q9RM63   11
 amylosucrase (Ams;NpAS) 2.4.1.4 Neisseria polysaccharea ATCC 43768 AAT15258.1
AAW13710.1
ACK26675.1
CAA03058.1
CAA03083.1
CAA09772.1
E2PEX2
Q9ZEU2
1G5A[A]
1JG9[A]
1JGI[A]
1MVY[A]
1MW0[A]
1MW1[A]
1MW2[A]
1MW3[A]
1S46[A]
1ZS2[A]
3UEQ[A]
4FLO[A]
4FLQ[A]
4FLR[A]
4FLS[A]
4
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141 Nostoc flagelliforme ADM32915.1 E1ANS4   10
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Nostoc punctiforme IAM M-15 BAG85337.1 B6ZIV0   26
 maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.141 Nostoc punctiforme IAM M-15 BAG85336.1 B6ZIU9   10
 debranching enzyme (Npun_R6073) 2.4.1.25
3.2.1.33
Nostoc punctiforme PCC 73102 ACC84362.1
CAY02945.1
CAZ78799.1
B2IUW9 2WC7[A]
2WCS[A]
2WKG[A]
20
 α-amylase (Amy1) 3.2.1.1 Nostoc sp. PCC 7119 CAQ30277.1 B1VK33   5
 trehalose synthase (TreS) 5.4.99.16 Paenarthrobacter aurescens CGMCC 1.1892 ACL80570.1 B8YM30   16
 pulullanase type I (PulA;PbPulA) 3.2.1.41 Paenibacillus barengoltzii CAU904 AJP16551.1
WP_016313728.1
    14
 a/β-cyclodextrin glucanotranferase (Cgt) (fragment) 2.4.1.19 Paenibacillus graminis MC22.13 ACA01964.1 B2XY82   2
 β-cyclodextrin glucanotransferase (CGTase) 2.4.1.19 Paenibacillus illinoisensis ZY-08 / ZY-8 ACO70966.1 C9WB02   2
 α-cyclodextrin glucanotransferase 2.4.1.19 Paenibacillus macerans   P31835   2
 α-cyclodextrin glucanotransferase (partial) 2.4.1.19 Paenibacillus macerans 602-1 AGT21379.1   3WMS[A] 2
 α-cyclodextrin glucanotransferase (CD;Cgt;CgtM) 2.4.1.19 Paenibacillus macerans IAM1243/ IB7 / IFO 3490 (NRRL B-388) / JFB05-01 (CCTCC M203062) CAA41773.1
AAA22298.1
AAC04359.1
O52766
P04830
4JCL[A] 2
 β-cyclodextrin glycosyltransferase (CGTase) 2.4.1.19 Paenibacillus pabuli US132 CAO05752.1     2
 multidomain α-amylase / β-amylase 3.2.1.1
3.2.1.2
Paenibacillus polymyxa AAA85446.1
CAA68344.1
P21543   1
 α-amylase (formerly neopullulanase) 3.2.1.1 Paenibacillus polymyxa CECT 155 AAD05199.1
CAZ78891.1
Q9ZAP8   36
 pullulanase type I (PulN) 3.2.1.41 Paenibacillus polymyxa Nws-pp2 AIE88189.1     14
 cyclodextrinase 3.2.1.54 Paenibacillus sp. A11 AAO47386.1
CAY02965.1
CAZ78882.1
Q84AP9   20
 β-cyclodextrin glucanotransferase 2.4.1.19 Paenibacillus sp. A11 AAG31622.1 Q9F5W3   2
 cyclodextrin glucanotransferase 2.4.1.19 Paenibacillus sp. JB-13 ACB71089.1 B2D1U4   2
 α-amylase / neopullulanase (Npl) 3.2.1.1 Paenibacillus sp. KCTC8848P AAL07400.1
CAZ78884.1
CBF79194.1
Q93C66   36
 isomaltulose synthase 5.4.99.11 Pantoea dispersa UQ68J AAP57083.1 Q6XNK6   31
 maltogenic amylase (MA;GcaTK4MA) 3.2.1.133 Parageobacillus caldoxylosilyticus TK4 ACN79585.1 C0LZ63   20
 oligo-α-1,6-glucosidase (MalL) 3.2.1.10 Parageobacillus thermoglucosidasius KP1006 BAA01368.1
CBF79249.1
P29094   31
 sucrose isomerase (PCSI;SIase) 5.4.99.11 Pectobacterium carotovorum KKH 3-1 AGT14432.1     31
 isoamylase (GlgX) 3.2.1.68 Pectobacterium carotovorum subsp. carotovorum LY34 ABV71062.1
ACN29676.1
A9XZV3
C0JID0
  11
 glycogen branching enzyme (GlgB) 2.4.1.18 Pectobacterium carotovorum subsp. carotovorum LY34 ACN29675.1 C0JIC9   9
 α-glucosidase (Agl) 3.2.1.20 Pediococcus pentosaceus PPE1.0 AAA25570.1
CAA83671.1
CBF79225.1
P43473   31
 sucrose phosphorylase 2.4.1.7 Pelomonas saccharophila IAM 14368 AAD40317.1 Q9XBR0   18
 maltotetraose-forming amylase (MTA;PSA) 3.2.1.60 Pelomonas saccharophila IAM1504 CAA34708.1
ABN31435.1
ABN31436.1
ABN31437.1
ABN31438.1
ABN31439.1
ABN31440.1
ABN31441.1
ABN31443.1
P22963  
 trehalose synthase (TreS) 5.4.99.16 Pimelobacter sp. R48 BAA11303.1
CBF79254.1
P72235   16
 trehalose synthase (TreS) 5.4.99.16 Propionibacterium freudenreichii subsp. shermanii NIZO B365 ABC88240.1
AAV63798.1
A1XGB1   16
 sucrose isomerase (SmuA) 5.4.99.11 Protaminobacter rubrum CBS 547.77 CAF32988.1
AAE36427.1
AAY69568.1
AAY69572.1
ACW35802.1
ACW35805.1
CAF32985.1
  3GBD[A]
3GBE[A]
31
 amylosucrase (AcAS;Achl_3605) 2.4.1.4 Pseudarthrobacter chlorophenolicus A6 ACL41561.1 B8H6N5   4
 α-amylase 3.2.1.1 Pseudoalteromonas haloplanktis ANT/505 AAO72321.1 Q84CG0   32
 α-amylase (Amy;AHA) 3.2.1.1 Pseudoalteromonas haloplanktis TAB23 / A23 CAA41481.1 P29957 1AQH[A]
1AQM[A]
1B0I[A]
1G94[A]
1G9H[A]
1JD7[A]
1JD9[A]
1KXH[A]
1L0P[A]
32
 isoamylase (Iam;IsoA) 3.2.1.68 Pseudomonas amyloderamosa SB-15 AAA25854.1
CAA31754.1
P10342 1BF2[A] 11
 trehalose-6-phosphate hydrolase (TreA) 3.2.1.93 Pseudomonas fluorescens ATCC 17400 AAG31032.1 Q9F8X2   29
 trehalose-6-phosphate hydrolase (TreA) 3.2.1.93 Pseudomonas fluorescens Q8R1-96 AAN06917.1
CBF79185.1
Q8GLN5   29
 sucrose isomerase / trehalulose synthase (MutB) 5.4.99.11 Pseudomonas mesoacidophila MX-45 ABC33903.1
AAY69574.1
ABT00107.1
ACO05018.1
CAF32995.1
Q2PS28 1ZJA[A,B]
1ZJB[A,B]
2PWD[A,B]
2PWE[A,B]
2PWF[A,B,C,D]
2PWG[A,B]
2PWH[A,B]
4GI6[A,B]
4GI8[A,B]
4GI9[A,B]
4GIA[A,B]
4GIN[A]
4GO8[A,B]
4GO9[A,B]
4H2C[A]
4H7V[A,B]
4H8H[A,B]
4H8U[A,B]
4H8V[A,B]
4HA1[A,B]
31
 palatinase / trehalulose hydrolase (MutA) 3.2.1.10
3.2.1.-
Pseudomonas mesoacidophila MX-45 CAA02858.1
AAE36430.1
AAY69575.1
ACO05017.1
ACO05017.2
C1K2X6   31
 isoamylase (Iam) 3.2.1.68 Pseudomonas sp. AAA25855.1
AAQ71617.1
AAQ71618.1
CAA00929.1
P26501   11
 α-amylase (AmyP) 3.2.1.1 Pseudomonas sp. 7193 CAM98700.1 A5CVD5  
 α-amylase 1 (Amy1) (alkaline resistant) 3.2.1.1 Pseudomonas sp. KFCC10818 AAA86836.1 Q52414   27
 α-amylase 2 (Amy2) 3.2.1.1 Pseudomonas sp. KFCC10818 AAA86835.1 Q52413   32
 α-amylase 4 (Amy4) 3.2.1.1 Pseudomonas sp. KFCC10818 AAK17994.1 Q9AGG4   32
 α-amylase 3 (Amy3) 3.2.1.1 Pseudomonas sp. KFCC10818 AAK17993.1 Q9AGG5   27
 maltopentaose-forming α-amylase (frameshift) 3.2.1.- Pseudomonas sp. KO-8940 BAA01600.1 Q52516  
 trehalose synthase (TreS) 5.4.99.16 Pseudomonas stutzeri CJ38 AAF26837.1
AAW06912.1
Q9LAS5   33
 maltotetraose-forming amylase (AmyP) 3.2.1.60 Pseudomonas stutzeri MO-19 AAA25707.1
ABN31444.1
ABN31445.1
ABN31446.1
ABN31447.1
ABN31448.1
ABN31449.1
ABN31450.1
ABN31451.1
ABN31452.1
P13507 1AMG[nd]
1GCY[A]
1JDA[A]
1JDC[A]
1JDD[A]
1QI3[A]
1QI4[A]
1QI5[A]
1QPK[A]
2AMG[A]
 pullulanase 3.2.1.41 Pullulanibacillus naganoensis AAQ80298.1
AAQ80294.1
AAQ80299.1
AAQ80300.1
AEV53626.1
    14
 pullulanase (PulA;HMPREF9690_00740) 3.2.1.41 Raoultella ornithinolytica 10-5246 EHT13411.1   2YOC[A,B] 13
 isomaltulose synthase 5.4.99.11 Raoultella planticola AAP57085.1
CBF79174.1
Q6XKX6   31
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Rhizobium sp. M-11 BAA11186.1 Q53237   26
 Malto-oligosyltrehalose trehalohydrolase 3.2.1.141 Rhizobium sp. M-11 AAE13737.1
AAE13729.1
AAE15469.1
AAE15477.1
BAA11187.1
Q53238   10
 trehalose synthase (TreS;ROP_61000) 5.4.99.16 Rhodococcus opacus B4 BAH54347.1 C1AZS6   16
 isoamylase 3.2.1.68 Rhodothermus marinus AAN89211.1
AAE45696.1
AAE45700.1
AAN89215.1
    11
 branching enzyme (GlgB;RoBE) 2.4.1.18 Rhodothermus marinus JCM9785 BAB69858.1
AAR45540.1
Q93HU3   9
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