Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54252); Uniprot accession (7858); PDB accession (465); 3D entries (110); cryst (2)
All (49476) Archaea (367) Bacteria (45982) Eukaryota (2997) unclassified (130) Structure (110 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 α-amylase (AmyB) (Amy13A) 3.2.1.1 Roseburia sp. A2-194 CAJ20070.1 Q3LB10   41
 α-amylase 2 (AmyA) 3.2.1.1 Salmonella typhimurium SJW1103 AAA27110.1
AAA27079.1
AAA71970.1
P26613   5
 sucrose isomerase 5.4.99.11 Serratia plymuthica ATCC 15928 CAF32996.1
CAF32990.1
    31
 STHERM_c15980 3.2.1.1 Spirochaeta thermophila DSM 6192 ADN02538.1 E0RN69   6
 α-1,4-glucosidase (MalA) 3.2.1.20 Staphylococcus xylosus CAA55409.1 Q60015   31
 pullulanase type I (Sap;BibA;SAG1216;SAG_1216) 3.2.1.41 Streptococcus agalactiae 2603V/R AAN00098.1
NP_688225.1
    12
 α-amylase I (AmyA;Amy I) 3.2.1.1 Streptococcus equinus 148 BAA24177.1 O50582   28
 α-amylase II (Amy II) 3.2.1.1 Streptococcus equinus 148 BAA24178.1 O50583   5
 α-amylase 3.2.1.1 Streptococcus equinus JB1 AAA97431.1 Q53786   5
 dextran α-1,6-glucosidase (DexB;SmDG) 3.2.1.70 Streptococcus mutans ATCC 25175 BAE79634.1 Q2HWU5 2ZIC[A]
2ZID[A]
31
 dextran α-1,6-glucosidase (DexB) 3.2.1.70 Streptococcus mutans INGBRITT AAA26939.1 Q99040   31
 sucrose phosphorylase (GftA;SmSP) 2.4.1.7 Streptococcus mutans INGBRITT / GS5 CAA30846.1
AAA26937.1
P10249   18
 α-amylase (intracellular) (Amy) 3.2.1.1 Streptococcus mutans LT11 AAC35010.1 O68875   5
 sucrose phosphorylase (GtfA;SMU.881) 2.4.1.7 Streptococcus mutans UA159 AAN58596.1
NP_721290.1
P10249   18
 α-amylase (AmyA;SMU.1590) 3.2.1.1 Streptococcus mutans UA159 AAN59233.1
NP_721927.1
    5
 pullulanase (SpuA) 3.2.1.41 Streptococcus pneumoniae 3.B AAG33958.1 Q9F930   12
 glycogen-degrading enzyme (SpuA;PulA;SP0268) 3.2.1.- Streptococcus pneumoniae TIGR4 AAK74446.1
AAQ38066.1
AAT16713.1
AAY71712.1
ABA33035.1
ACK14503.1
ACW45222.1
NP_344806.1
Q97SQ7 2YA0[A]
2YA1[A]
2YA2[A]
12
 MGAS10750_Spy1875 (DexS) 3.2.1.93 Streptococcus pyogenes MGAS10750 ABF38825.1 Q1J4B1   29
 cyclomaltodextrin glucanotransferase (AmyA;M5005_Spy_1065) 2.4.1.19 Streptococcus pyogenes MGAS5005 AAZ51683.1     2
 Spy49_1626c (PulA) 3.2.1.41 Streptococcus pyogenes NZ131 ACI61883.1
CAD32942.1
B5XIM1
Q8KLP1
  12
 bifunctional amylopullulanase (ApuA;SSU1849) 3.2.1.1
3.2.1.41
Streptococcus suis P1/7 CAR47543.1 C5VVZ9   41
 bifunctional amylopullulanase (ApuA;SSU1849) 3.2.1.1
3.2.1.41
Streptococcus suis P1/7 CAR47543.1 C5VVZ9   12
 α-amylase 3.2.1.1 Streptomyces albus AAA96317.1 Q53633   32
 α-amylase (SAV5981) 3.2.1.1 Streptomyces avermitilis MA-4680 = NBRC 14893 BAC73693.1
NP_827158.1
    32
 α-1,4-glucan: phosphate α-maltosyltransferase I (GlgE;SCO5443;SC6A11.19C) 2.4.99.16 Streptomyces coelicolor A3(2) CAB72419.1
CAA04600.1
NP_629581.1
Q9L1K2 3ZSS[A,B,C,D]
3ZST[A,B]
3ZT5[A,B,C,D]
3ZT6[A,B,C,D]
3ZT7[A,B,C,D]
4CN1[A,B]
4CN4[A,B]
4CN6[A,B]
4U2Y[A,A,B,B]
4U2Z[A,B]
4U31[A,B]
5CVS[A,B]
5LGV[A,B]
5LGW[A,B]
5VSJ[A,B]
5VT4[A,B,C,D]
3
 branching enzyme I (GlgBI;SC6A11.16c;SCO5440) 2.4.1.18 Streptomyces coelicolor A3(2) CAA58314.1
CAB72416.1
NP_629578.1
Q59833   9
 branching enzyme II (GlgBII;SC4G10.11c;SCO7332) 2.4.1.18 Streptomyces coelicolor A3(2) CAB92878.1
CAA52109.1
NP_631386.1
Q59832   9
 α-amylase (Amy) 3.2.1.1 Streptomyces griseus IMRU3570 CAA40798.1 P30270   32
 α-amylase 3.2.1.1 Streptomyces hygroscopicus AAA26698.1 P08486   32
 α-amylase (Aml) 3.2.1.1 Streptomyces limosus ATCC 19778 AAA88554.1 P09794   32
 α-amylase (Aml;AmlA) 3.2.1.1 Streptomyces lividans TK21 CAA73926.1 O86876   32
 α-amylase (AmlB) 3.2.1.1 Streptomyces lividans TK24 CAB06622.1 P97179   32
 α-amylase (Amy) 3.2.1.1 Streptomyces lividans TK24 CAA49759.1 Q05884  
 α-amylase (AmlC) 3.2.1.1 Streptomyces lividans TK24 CAB06816.1 P96992   42
 α-amylase 3.2.1.1 Streptomyces sp. TO1 CAA73775.1 O65947   32
 α-amylase (Amy) 3.2.1.1 Streptomyces sp. WL6 AAA82875.1 Q59964   32
 α-amylase 3.2.1.1 Streptomyces thermoviolaceus CUB74 AAA26697.1 P27350   32
 SVEN_5096 (TreS) 5.4.99.16 Streptomyces venezuelae ATCC 10712 CCA58382.1     16
 SVEN_5097 (GlgE) 2.4.99.16 Streptomyces venezuelae ATCC 10712 CCA58383.1     3
 α-amylase (Aml) 3.2.1.1 Streptomyces venezuelae ATCC 15068 AAB36561.1 P22998   32
 isoamylase (GlgX;Synpcc7942_0086) 3.2.1.68 Synechococcus elongatus PCC 7942 ABB56118.1 Q31S51   11
 α-1,4-glucan branching enzyme (ScoBE;Synpcc7942_1085) 2.4.1.18 Synechococcus elongatus PCC 7942 ABB57115.1 P16954   9
 amylosucrase (AmsA;1986-orf;AMS;SYNPCC7002_A0885) 2.4.1.4 Synechococcus sp. PCC 7002 ACA98889.1
CDL67995.1
B1XIU7   4
 α-amylase (AmyTV1;AmyE) 3.2.1.1 Thermoactinomyces vulgaris K94 CAA49465.1
CAZ78876.1
Q60051   1
 α-amylase I (TVA I) 3.2.1.1 Thermoactinomyces vulgaris R-47 BAA02471.1
CAY02971.1
CAZ78902.1
Q60053 1JI1[A,B]
1UH2[A]
1UH3[A]
1UH4[A]
2D0F[A]
2D0G[A]
2D0H[A]
21
 cyclomaltodextrinase / neopullulanase (TVA II;TvCMD) 3.2.1.135
3.2.1.54
Thermoactinomyces vulgaris R-47 BAA02473.1
BAA97040.1
BAB40638.1
CAY02972.1
CAZ78903.1
Q08751
Q7DI93
1BVZ[A,B]
1G1Y[A,B]
1JF5[A,B]
1JF6[A,B]
1JI2[A,B]
1JIB[A,B]
1JL8[A,B]
1VB9[A,B]
1VFK[A,B]
1VFM[A,B]
1VFO[A,B]
1VFU[A,B]
1WZK[A,B]
1WZL[A,B]
1WZM[A,B]
2D2O[A,B]
3A6O[A,B]
20
 cyclodextrinase (CDase) 3.2.1.54 Thermoanaerobacter ethanolicus ATCC 33223 / 39E AAA23219.1
CAY02970.1
CAZ78899.1
P29964   20
 amylase-pullulanase (Apu;Apu-Te;TrAPU;Teth39_2173) 3.2.1.1
3.2.1.41
Thermoanaerobacter pseudethanolicus ATCC 33223 ABY95795.1
AAA23201.1
AAV00812.1
P38939
P38939
  39
 α-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Thermoanaerobacter sp. 5K AJE25826.1     2
 a/β-cyclodextrin glucanotransferase 2.4.1.19 Thermoanaerobacter sp. ATCC 53627 CAJ84380.1     2
 a/β-cyclodextrin glucanotransferase (Cgt) 2.4.1.19 Thermoanaerobacter sp. P4 AJE25827.1     2
 amylase-pullulanase (Apu) 3.2.1.1
3.2.1.41
Thermoanaerobacter thermohydrosulfuricus AAA23205.1
CAZ78897.1
P16950   39
 amylase-pullulanase (Apu;Apu-Ts) 3.2.1.1
3.2.1.41
Thermoanaerobacterium saccharolyticum B6A-RI AAA19800.1 P36905   39
 amylopullulanase (Apu;Apu-Ts) (partial) 3.2.1.1
3.2.1.41
Thermoanaerobacterium saccharolyticum NTOU1 ACR15165.1     39
 6?-P-sucrose phosphorylase (SPP;TtSPP;Tthe_1921) 2.4.1.- Thermoanaerobacterium thermosaccharolyticum DSM 571 ADL69407.1 D9TT09   18
 amylase-pullulanase (AmyB) 3.2.1.1
3.2.1.41
Thermoanaerobacterium thermosulfurigenes EM1 AAB00841.1 P38536   39
 a/β-cyclodextrin glucanotransferase (AmyA) 2.4.1.19 Thermoanaerobacterium thermosulfurigenes EM1 AAB00845.1 P26827 1A47[A]
1CIU[A]
3BMV[A]
3BMW[A]
2
 maltotriose-producing α-amylase (Tfa;Amy13) 3.2.1.1
3.2.1.116
Thermobifida fusca NTU22 ABF13430.1 Q1KLC8   32
 trehalose synthase (TreS;Tfu_0584) 5.4.99.16 Thermobifida fusca YX AAZ54622.1
CAZ78814.1
CBF79133.1
Q47SE5   16
 α-glucosidase (Tfu_0833) 3.2.1.20 Thermobifida fusca YX AAZ54871.1 Q47RP6   30
 isoamylase (Tfu_1891) 3.2.1.68 Thermobifida fusca YX AAZ55924.1 Q47NP5   11
 α-amylase (Tam) 3.2.1.1 Thermomonospora curvata CAA41881.1 P29750   32
 α-glucosidase (AglA) 3.2.1.20 Thermomonospora curvata AAA57313.1 Q60027   30
 4-α-glucanotransferase (Mgt;TM0364) 2.4.1.25 Thermotoga maritima MSB8 CAA90693.1
AAD35451.1
AGL49287.1
AHD17874.1
AKE28162.1
NP_228175.1
P80099
Q60035
1LWH[A,B]
1LWJ[A,B]
 4-α-glucanotransferase / maltosyltransferase (MTase;TM0767) 2.4.1.25
2.4.1.-
Thermotoga maritima MSB8 AAD35849.1
AGL49693.1
AHD17478.1
AKE28548.1
CAA04525.1
NP_228576.1
O33838
Q9S5X2
1GJU[A]
1GJW[A]
 α-amylase (AmyA;TM1840;Tmari_1848) (Amy13A) 3.2.1.1 Thermotoga maritima MSB8 CAA72194.1
AAD36902.1
AGL50772.1
AHD18270.1
AKE29589.1
CAZ78695.1
CBF78892.1
NP_229636.1
G4FGM5
P96107
Q9S5Y2
  36
 α-amylase (AmyB;TM1650) (Amy13B) 3.2.1.1 Thermotoga maritima MSB8 AAD36717.1
AGL50583.1
AHD18453.1
AKE29408.1
NP_229450.1
Q9X1Y3  
 cyclomaltodextrinase (AglB;TM1835) 3.2.1.54 Thermotoga maritima MSB8 AAD36898.1
AGL50768.1
AHD18274.1
AKE29585.1
CAY02913.1
CAZ78694.1
NP_229632.1
G4FGM1
Q9X2F4
  20
 pullulanase (PulA;Tm1845) (Pul13A) 3.2.1.41 Thermotoga maritima MSB8 AAD36907.1
AAM56795.1
ABJ38302.1
ABJ38322.1
ACW20067.1
ACW20078.1
AGL50777.1
AHD18265.1
AKE29594.1
CAA04522.1
CAH68696.1
CAT81460.1
NP_229641.1
G4FGN0
O33840
  14
 4-α-glucanotransferase (MgtA) 2.4.1.25 Thermotoga neapolitana CAA08864.1
CAZ78901.1
O86956  
 cyclomaltodextrinase glucanotransferase (MalA;AglB) 3.2.1.54 Thermotoga neapolitana CAA08867.1
CAY02961.1
CAZ78866.1
O86959   20
 α-amylase (AmyE;CTN_0781) 3.2.1.1 Thermotoga neapolitana DSM 4359 AAF37330.1 Q9LCU1  
 maltogenic α-amylase (AmyB;CTN_0737) 3.2.1.133 Thermotoga neapolitana DSM 4359 ACM22913.1
ACF75909.1
B5ARZ9
B9K7I0
4GKL[A,B]
 pullulanase (PulA) 3.2.1.41 Thermotoga neapolitana KCCM 41025 ACN58254.1 C0LLG9   14
 trehalose synthase (TreS) 5.4.99.16 Thermus aquaticus ATCC 33923 BAA19934.1 O06458   16
 trehalose synthase (TreS) 5.4.99.16 Thermus caldophilus GK24 AAD50660.3
AAD50660.1
Q9RA59   16
 α-glucosidase 3.2.1.20 Thermus caldophilus GK24 AAD50603.1
CBF79218.1
Q9RA62   23
 cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA) 3.2.1.133
3.2.1.54
Thermus sp. IM6501 AAC15072.1 O69007 1GVI[A]
1SMA[A]
20
 pullulanase (Pul) 3.2.1.41 Thermus sp. IM6501 AAC15073.1 O69008   14
 α,α-trehalase / α-glucosidase (TTC0107) 3.2.1.20
3.2.1.28
Thermus thermophilus HB27 AAS80455.1
CBF79062.1
YP_004082.1
Q72LF2   23
 pullulanase (TTHpu;TTC1198) (fragment) 3.2.1.41 Thermus thermophilus HB27 AAS81540.1
CAZ78778.1
YP_005167.1
Q72ID4   20
 α-glucosidase (TTHA0481) 3.2.1.20 Thermus thermophilus HB8 BAD70304.1
AAW21751.1
Q5SL12   23
 trehalose synthase (TTHA0478) 5.4.99.16 Thermus thermophilus HB8 BAD70301.1
AAW21753.1
Q5SL15   16
 pullulanase (Pul) 3.2.1.41 Thermus thermophilus HB8 BAB62095.1 Q7DI19   14
 trehalose synthase (TreS;TtTS) 5.4.99.16 Thermus thermophilus HJ6 ABW87308.1 A8WAD0   16
 α-glucosidase (TtAG) 3.2.1.20 Thermus thermophilus TC11 AKA95141.1     23
 α-amylase (AmyA) 3.2.1.1 uncultured bacterium AAP49335.1
CBF79177.1
Q6XR91   36
 α-1,4-glucan branching enzyme (AmyB) 2.4.1.18 uncultured bacterium AAP70397.1 Q6XE44   9
 α-amylase (AmyM) 3.2.1.1 uncultured bacterium AAQ89599.1
ALJ10569.1
CAZ78880.1
CBF79168.1
Q6TXT5   36
 α-amylase (AmyP;Amy-1E) 3.2.1.1 uncultured bacterium AEM89278.1
ADK21254.1
  5H05[A,B,C,D]
5H06[A,B,C,D]
37
 α-amylase (Amy13A) 3.2.1.1 uncultured bacterium CCD30600.1     5
 amylopullulanase (Ss4;PulSS4) 3.2.1.1
3.2.1.41
uncultured bacterium AGL50935.1     12
 amylopullulanase (Ss4;PulSS4) 3.2.1.1
3.2.1.41
uncultured bacterium AGL50935.1     28
 cyclodextrinase (Amy1) 3.2.1.54 uncultured bacterium ACA48223.1 B1PHW8   20
 neopullulanase (Amy29) 3.2.1.135 uncultured bacterium ACA48224.1 B1PHW9   20
 cyclodextrinase (Amy132) 3.2.1.54 uncultured bacterium ACA48225.1 B1PHX0   20
 amylase (PLA;AmyPL) 3.2.1.1 uncultured bacterium AHB39934.1     28
 a/β-cyclodextrin glycosyltransferase (Cgt13;CspCGT13) (partial) 2.4.1.19 uncultured Carboxydocella sp. AIL25792.1     2
 cyclomaltodextrinase (CdaA) (Cda13A) 3.2.1.54 uncultured soil bacterium ABA39290.1 Q3HW59   20
 maltopentaose-forming amylase 3.2.1.- unidentified bacterium UGO163/26 / DSM 5853 CAA37453.1 Q03658   19
Top


Last update: 2017-10-09 © Copyright 1998-2017
AFMB - CNRS - Université d'Aix-Marseille