Glycoside Hydrolase Family 13

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (54252); Uniprot accession (7858); PDB accession (465); 3D entries (110); cryst (2)
All (49476) Archaea (367) Bacteria (45982) Eukaryota (2997) unclassified (130) Structure (110 - 2 cryst) Characterized (812)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 α-glucosidase (AglB;AglA;BAD_0452) 3.2.1.20 Bifidobacterium adolescentis ATCC 15703 AAL05573.1
BAF39233.1
CBF79196.1
A1A0K0
Q93CA0
  30
 sucrose phosphorylase (SucP;SP;BaSP) 2.4.1.7 Bifidobacterium adolescentis DSM 20083 AAO33821.1 Q84HQ2 1R7A[A,B]
2GDU[A,B]
2GDV[A,B]
5C8B[B]
18
 oligo α-glucosidase (AglA) 3.2.1.10 Bifidobacterium adolescentis DSM 20083 AAK27723.1 Q9AF93   31
 α-amylase (AmyB) 3.2.1.1 Bifidobacterium adolescentis INT-57 AAP69820.1 Q5JB42   28
 α-1,6-glucosidase 1 (Agl1; Bbr_1855) 3.2.1.10
3.2.1.20
Bifidobacterium breve UCC2003 ACM89182.1
ABE96517.1
C0JP82   31
 α-1,6-glucosidase 2 (Agl2; Bbr_0559) 3.2.1.10
3.2.1.20
Bifidobacterium breve UCC2003 ACM89183.1
ABE95255.1
C0JP83   31
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.1
3.2.1.41
Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1
F9Y041   14
 bifunctional amylopullulanase (ApuB;Bbr_0123) 3.2.1.1
3.2.1.41
Bifidobacterium breve UCC2003 AAY89038.1
ABE94835.1
F9Y041   32
 sucrose phosphorylase (Spl;BL0536) 2.4.1.7 Bifidobacterium longum NCC2705 AAN24362.1
NP_695726.1
Q8G6U7   18
 sucrose phosphorylase (SplP) 2.4.1.7 Bifidobacterium longum SJ32 AAO84039.1 Q84BY1   18
 sucrose phosphorylase (Spl) 2.4.1.7 Bifidobacterium longum subsp. longum JCM 1217 BAF62433.1     18
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116
Brachybacterium sp. LB25 BAE94180.1 Q1JUA3   32
 β-cyclodextrin glycosyltransferase (Cgt) 2.4.1.19 Brevibacillus brevis CD162 AAB65420.1 O30565   2
 maltooligosyltrehalose synthase (TreY;BvMts) 5.4.99.15 Brevibacterium helvolum AAB95368.1 O52519   26
 maltooligosyltrehalose trehalohydrolase (TreZ;BvMth) 3.2.1.141 Brevibacterium helvolum AAB95369.1 O52520   10
 α-amylase (AmyA) (Amy13B) 3.2.1.1 Butyrivibrio fibrisolvens 16/4 CAE17325.1
CBK74052.1
Q70JA8   28
 α-amylase (AmyA) 3.2.1.1 Butyrivibrio fibrisolvens H17C AAA23005.1 P30269   28
 branching-enzyme (GlgB) 2.4.1.18 Butyrivibrio fibrisolvens H17C AAA23007.1 P30539   9
 α-maltosyltransferase (MTase;Athe_0143;cbes0143) 2.4.1.- Caldicellulosiruptor bescii DSM 6725 ACM59297.1 B9MLT9  
 pullulanase type I (PulA) 3.2.1.41 Caldicellulosiruptor saccharolyticus AAB06264.1 Q59319   14
 branching enzyme (GlgB;CPn0475) 2.4.1.18 Chlamydophila pneumoniae CWL029 AAD18615.1
NP_224671.1
    9
 α-amylase (AmyA;AmyP;CAP0168) 3.2.1.1 Clostridium acetobutylicum ATCC 824 AAD47072.1
AAA63759.1
AAA63759.2
AAK76913.1
NP_149331.1
P23671   28
 maltohexaose-forming α-amylase (AmyM) 3.2.1.98 Corallococcus sp. EGB AII00648.1     6
 isoamylase (GlgX;Cgl2107;cg2310) 3.2.1.68 Corynebacterium glutamicum ATCC 13032 BAB99500.1
ACC05670.1
ACH05486.1
ACH05487.1
CAC25858.1
CAC25859.1
CAF20443.1
CAK31208.1
CAK31209.1
CCH25246.1
NP_601306.1
Q8NNT0   11
 maltooligosyl trehalose synthase (TreY;CgI2118;cg2323) 5.4.99.15 Corynebacterium glutamicum ATCC 13032 BAB99511.1
ABY16668.1
CAD43536.1
CAF20454.1
CCH25257.1
NP_601318.1
Q8NNR9   26
 maltooligosyltrehalose trehalohydrolase(TreZ;Cgl2125;cg2333) 3.2.1.141 Corynebacterium glutamicum ATCC 13032 BAB99519.1
ACK11454.1
CAC25671.1
CAD43537.1
CAD88152.1
CAF20463.1
CCH25265.1
NP_601327.2
Q8NNR1   10
 1,4-α-glucan branching enzyme (Glgb2) 2.4.1.18 Cyanobacterium sp. NBRC 102756 ABO93392.1 A4UU35   9
 1,4-α-glucan branching enzyme (Glgb3) 2.4.1.18 Cyanobacterium sp. NBRC 102756 ABO93393.1 A4UU36   9
 α-1,4-glucan branching enzyme (BE3;cceBE3;cce_1806) 2.4.1.18 Cyanothece sp. ATCC 51142 ACB51156.1 B1WZK4   9
 α-1,4-glucan branching enzyme (GlgB;BE1;cceBE1;cce_2248) 2.4.1.18 Cyanothece sp. ATCC 51142 ACB51598.1 B1WPM8 5GQU[A]
5GQV[A]
5GQW[A]
5GQX[A]
5GQY[A]
5GQZ[A]
5GR0[A]
5GR1[A]
5GR2[A]
5GR3[A]
5GR4[A]
5GR5[A]
5GR6[A]
9
 α-1,4-glucan branching enzyme (BE2;cceBE2;cce_4595) 2.4.1.18 Cyanothece sp. ATCC 51142 ACB53943.1 B1WVF3   9
 α-amylase (RSDA) (raw-starch-digesting) 3.2.1.1 Cytophaga sp. AAF00567.1 Q9RQT8   5
 trehalose synthase (TreS;Deide_07350) 5.4.99.16 Deinococcus deserti VCD115 ACO45593.1 C1D169   16
 glycogen branching enzyme (GlgB;GBE;DgGBE;Dgeo_0981) 2.4.1.18 Deinococcus geothermalis DSM 11300 ABF45281.1 Q1IZQ3   9
 amylosucrase (DgAS;Dgeo_0572) 2.4.1.4 Deinococcus geothermalis DSM 11300 ABF44874.1
YP_604043.1
Q1J0W0 3UCQ[A]
3UER[A]
4
 malto-oligosyltrehalose trehalohydrolase (DR0464) 3.2.1.141 Deinococcus radiodurans R1 AAF10042.1
ANC72786.1
NP_294187.1
Q9RX51 2BHU[A]
2BHY[A]
2BHZ[A]
2BXY[A]
2BXZ[A]
2BY0[A]
2BY1[A]
2BY2[A]
2BY3[A]
10
 amylosucrase (AS;DrAS;DR0933) 2.4.1.4 Deinococcus radiodurans R1 AAF10510.1
ANC71873.1
NP_294657.1
Q9RVT9 4AYS[A] 4
 glycogen branching enzyme (GlgB;DR_1848;DR1848) 2.4.1.18 Deinococcus radiodurans R1 AAF11402.1
ANC71059.1
NP_295571.1
Q9RTB7   9
 trehalose synthase (DrTS;DR2036) 5.4.99.16 Deinococcus radiodurans R1 AAF11586.1
AEJ36289.1
ANC70889.1
CAZ78703.1
CBF78903.1
NP_295759.1
Q9RST7 4TVU[A,B,C,D,E,F,G,H]
4WF7[A,B,C,D]
5GTV[A,B,C,D]
5GTW[A,B,C,D]
16
 glycogen branching enzyme (GlgB) 2.4.1.18 Dickeya chrysanthemi PY35 AAN64024.1
ABY54975.1
B0F2B5
Q8GQC5
  9
 α-amylase 2 (AmyB) 3.2.1.1 Dictyoglomus thermophilum CAA31586.1
CAZ78892.1
P14898   20
 α-amylase 3 (AmyC) 3.2.1.1 Dictyoglomus thermophilum CAA34072.1
CAZ78893.1
CBF79228.1
P14899   36
 trehalose synthase (TreS) 5.4.99.16 Enterobacter hormaechei 7 ACI16355.1 B6E9W1   16
 sucrose isomerase (EsI) 5.4.99.11 Enterobacter sp. FMB-1 ACF42098.1 B5ABD8   31
 α-glucosidase (PalZ) 3.2.1.20 Erwinia rhapontici DSM 4484 AAK28739.1
CAC69267.1
CAC69279.1
CBF79203.1
Q9AI60   31
 oligo-α-glucosidase / palatinase (PalQ) 3.2.1.10
3.2.1.-
Erwinia rhapontici DSM 4484 AAK28737.1
CAC69265.1
CAC69271.1
CBF79205.1
Q9AI62   31
 isomaltulose synthase (PalI) 5.4.99.11 Erwinia rhapontici NX-5 / DSM 4484 AAK28735.1
AAY69569.1
ACW35803.1
ADJ56407.2
CAC69266.1
CAC69272.1
CAC69285.1
CAF32986.1
CAF32987.1
D9MPF2
Q9AI64
4HOW[A]
4HOX[A]
4HOZ[A]
4HP5[A]
4HPH[A]
31
 isomaltulose synthase 5.4.99.11 Erwinia rhapontici WAC2928 AAP57084.1
ACR90553.1
Q6XNK5   31
 branching-enzyme (EcBE;GlgB;EcE24377A_3911) 2.4.1.18 Escherichia coli O139:H28 str. E24377A ABV17243.1 A7ZSW5 5E6Y[A,B,C,D]
5E6Z[A,B,C,D]
5E70[A,B,C,D]
9
 trehalose 6-P hydrolase (ECs5216) 3.2.1.93 Escherichia coli O157:H7 str. Sakai BAB38639.1
NP_313243.1
Q8XCE1   29
 α-amylase (MalS;b3571) 3.2.1.1 Escherichia coli str. K-12 substr. MG1655 AAB18548.1
AAC76595.1
AIZ93631.1
ALI38743.1
AMC96456.1
CAA41740.1
NP_418028.1
P25718   19
 α-amylase 2 (AmyAl;EAMY) 3.2.1.1 Escherichia coli str. K-12 substr. MG1655 AAC74994.1
AAA23810.1
AAA82575.1
AIZ90980.1
ALI40306.1
AMC94904.1
NP_416437.1
P26612   5
 α-glucosidase Z (EO53_16270;MalZ;b0403) 3.2.1.20 Escherichia coli str. K-12 substr. MG1655 AIZ92452.1
AAA16112.1
AAB40159.1
AAC73506.1
AAC73506.2
ALI41788.1
AMC97641.1
CAA42498.1
CAY02912.1
CAZ78690.1
NP_414937.1
P21517 5BN7[A] 21
 branching-enzyme (GlgB;EcoBE;b3432) 2.4.1.18 Escherichia coli str. K-12 substr. MG1655 AAA23872.1
AAA58230.1
AAC76457.1
ABX94236.1
AIZ89568.1
ALI38888.1
AMC96315.1
CAP11381.1
NP_417890.1
P07762 1M7X[A]
3O7Y[A,B,C,D]
3O7Z[A,B,C,D]
4LPC[A,B,C,D]
4LQ1[A,B,C,D]
9
 isoamylase (GlgX;b3431) 3.2.1.68 Escherichia coli str. K-12 substr. MG1655 AAC76456.1
AAA58229.1
AAA98735.1
AIZ89569.1
ALI38889.1
AMC96314.1
CAP11380.1
NP_417889.1
P15067 2WSK[A] 11
 sucrose phosphorylase (YcjM) 2.4.1.7 Escherichia coli str. K-12 substr. MG1655 AAC74391.2
AIZ91576.1
ALI40912.1
AMC98520.1
NP_415825.2
P76041   18
 trehalose-6-phosphate hydrolase (TreC) 3.2.1.93 Escherichia coli str. K-12 substr. MG1655 AAC77196.1
AAA24225.1
AAA97136.1
AAC43382.1
AIZ92996.1
ALI38108.1
AMC97092.1
NP_418660.1
P28904   29
 maltodextrin glucosidase (MalZ) 3.2.1.20 Escherichia coli str. K-12 substr. W3110 BAE76183.1
APC50696.1
    21
 maltogenic α-amylase (EUR_01860) 3.2.1.133 [Eubacterium] rectale DSM 17629 CBK89424.1 D6E1Y4   36
 multidomain α-amylase (EUR_21100) (cell-wall anchored) 3.2.1.1 [Eubacterium] rectale DSM 17629 CBK91127.1 D6DYI9   41
 pullulanase (Pul3YH5) 3.2.1.41 Exiguobacterium acetylicum a1 / YH5 AFL46505.1     14
 α-amylase (AmyH) 3.2.1.1 Exiguobacterium sp. DAU5 AFZ41193.1     5
 α-amylase (Amy-E) (partial) 3.2.1.1 Exiguobacterium sp. Sh3 AIW80140.1    
 pullulanase (Pul-SH3) 3.2.1.41 Exiguobacterium sp. Sh3 AJG04842.1     14
 pullulanase I (PulA) 3.2.1.41 Fervidobacterium pennivorans Ven5 AAD30387.1 Q9XDB5 cryst 14
 isoamylase (Iam) 3.2.1.68 Flavobacterium sp. AAB63356.1 O32611   11
 cyclomaltodextrinase (Cdase;FspCMD) 3.2.1.54 Flavobacterium sp. 92 CAD32957.1 Q8KKG0 3EDD[A,B]
3EDE[A,B]
3EDF[A,B]
3EDJ[A,B]
3EDK[A,B]
 pullulanase (GK2827) 3.2.1.41 Geobacillus kaustophilus HTA426 BAD77112.1 Q5KW24   14
 α-glucosidase (Gsj) 3.2.1.20 Geobacillus sp. HTA-462 BAE48285.1 Q33E90 2ZE0[A] 31
 α-amylase (AmyP) 3.2.1.1 Geobacillus sp. POT5 ABL77406.1 A8QL62  
 α-amylase (Am-Stearo) 3.2.1.1 Geobacillus stearothermophilus AAE12382.1     5
 pullulanase (PulT) 3.2.1.41 Geobacillus stearothermophilus 1808262A     14
 α-amylase (Ami;Amy;AmyUS100) 3.2.1.1
3.2.1.98
Geobacillus stearothermophilus US100 / ATCC 31195 AAB86961.1
AAN99912.1
ACH26104.1
CAB93517.1
CCB84710.1
O31193
Q9KWY6
  5
 branching-enzyme (GlgB) 2.4.1.18 Geobacillus stearothermophilus 1503-4R AAA22482.1 P30538   9
  α-amylase / high-molecular-mass amylase (Hmma;HmwA) 3.2.1.1 Geobacillus stearothermophilus ATCC 12980 ABR26448.1 A6N9J4   39
 α-glucosidase / exo-α-1,4-glucosidase 3.2.1.20 Geobacillus stearothermophilus ATCC12016 BAA12704.1
CBF79155.1
P94451   31
 maltogenic α-amylase (BSuA;Novamyl) 3.2.1.133 Geobacillus stearothermophilus C599 ABA38666.1
AAA22233.1
AAY11155.1
ALJ77020.1
CAL60144.1
CAZ67881.1
P19531 1QHO[A]
1QHP[A]
2
 α-glucan branching enzyme (GlgB) (fragment) 2.4.1.18 Geobacillus stearothermophilus CU21 CAA79749.1 Q59243   9
 α-amylase (AmyS) 3.2.1.1 Geobacillus stearothermophilus DY5 / PHI300 / NZ-3 AAA22235.2
AAA22227.1
AAA22241.1
AAE13948.1
AAE37102.1
AAE59725.1
AAE62347.1
AAN20004.1
AAN27446.1
AAP02209.1
AAR63624.1
AAS26289.1
AAY06018.1
AAY72171.1
ABJ43096.1
ABN29370.1
ACK07342.1
ACP75067.1
ACW26902.1
CAA26547.1
CAC16485.1
CAC88549.1
CAD19033.1
CAD20676.1
CAD26700.1
CAD67515.1
P06279 1HVX[A]
4UZU[A]
5
 β-cyclodextrin glucanotransferase (Cgt;BsCgt) 2.4.1.19 Geobacillus stearothermophilus ET1 AAD00555.1 Q9ZAQ0   2
 maltogenic α-amylase (BSMA) 3.2.1.133 Geobacillus stearothermophilus ET1 AAC46346.1 Q45490   20
 α-amylase 3.2.1.1 Geobacillus stearothermophilus GRE1 ABC18196.1     5
 neopullulanase 3.2.1.135 Geobacillus stearothermophilus IMA6503 AAK15003.1 Q9AIV2   20
 a/β-cyclodextrin glucanotransferase (Cgt;CgtS;Aga2) 2.4.1.19 Geobacillus stearothermophilus No. 2 / NO2 CAA41770.1
CAA41771.1
CAA41772.1
P31797 1CYG[A] 2
 α-amylase 3.2.1.1 Geobacillus stearothermophilus TISTR 329 AAU10478.1 Q66T78   5
 branching-enzyme (GlgB) 2.4.1.18 Geobacillus stearothermophilus TRBE14 BAA19588.1 Q59242   9
 cyclomaltodextrinase / neopullulanase (NplT;BsCMD) 3.2.1.135
3.2.1.54
Geobacillus stearothermophilus TRS40 AAA22622.1 P38940 1J0H[A,B]
1J0I[A,B]
1J0J[A,B]
1J0K[A,B]
20
 amylopullulanase (ApuTS) 3.2.1.1
3.2.1.41
Geobacillus stearothermophilus TS-23 AAG44799.1
CAZ78886.1
Q9EZZ4   39
 α-amylase (GA) 3.2.1.1 Geobacillus thermodenitrificans LH8 ABX83871.1 A9UJ60   5
 α-amylase (GTA;GTCCBUS3UF5_7870) 3.2.1.1 Geobacillus thermoleovorans CCB_US3_UF5 AEV18110.1   4E2O[A]
 amylopullulanase (Apu;Apu105;Gt-Apu) 3.2.1.1
3.2.1.41
Geobacillus thermoleovorans NP33 AFI70750.1     39
 α-amylase II (Amy2;Gt-AmyII) 3.2.1.1
3.2.1.133
Geobacillus thermoleovorans NP54 AFK08971.1    
 maltogenic-amylase (Amy3;Gt-Mamy) 3.2.1.133 Geobacillus thermoleovorans NP54 AFM43699.1     20
 α-amylase (Amy;Gt-Amy) 3.2.1.1 Geobacillus thermoleovorans NP54 AFC87833.1     5
 α-amylase (AmyA) (raw-starch digesting) 3.2.1.1
3.2.1.133
Geobacillus thermoleovorans subsp. stromboliensis Pizzo ADG45817.1 D6R179  
 pullulanase type I (Orf1) 3.2.1.41 Geobacillus thermoleovorans US105 CAC85704.1 Q8GJ67   14
 α-amylase (AmyH) 3.2.1.1 Halomonas meridiana DSM 5425 CAB92963.1 Q9L4I9   32
 α-glucosidase (AglA;HaG) 3.2.1.20 Halomonas sp. H11 BAL49684.1   3WY1[A,B]
3WY2[A,B]
3WY3[A,B]
3WY4[A,B]
23
 α-amylase A (AmyA;Hore_14740) 3.2.1.1 Halothermothrix orenii H 168 AAN52525.1
ACL70223.1
CAZ78883.1
CBF79188.1
B8CY54
Q8GPL8
1Q76[nd]
1WML[nd]
1WZA[A]
36
 α-amylase B (AmyB) 3.2.1.1 Halothermothrix orenii H 168 ACL70573.1 B8CZ54 3BC9[A]
3BCD[A]
3BCF[A]
5
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