Known Activities	α-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); amino acid transporter.
Mechanism	Retaining
Clan	GH-H
3D Structure Status	( β / α ) 8
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	AAMY; EBI Protein of the Month; HOMSTRAD; InterPro; PDB Molecule of the Month; PFAM; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (9773); Uniprot accession (4347); PDB accession (301); 3D entries (69); cryst (6)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
α-amylase (AmyA)	3.2.1.1	Pyrococcus woesei	AAD54338.1 AAF44693.1 ACH26211.1	Q7LYT7 Q9P9L0	1MWO[A] 1MXD[A] 1MXG[A]	7
maltooligosyltrehalose synthase (TreY)	5.4.99.15	Sulfolobus acidocaldarius	BAA11009.1 BAA11865.1	Q53688	1IV8[A]	26
α-amylase / maltooligosyltrehalose trehalohydrolase	3.2.1.1 3.2.1.141	Sulfolobus solfataricus KM1	AAR46454.1 BAA11010.1	Q55088	1EH9[A] 1EHA[A]	10
isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021)	2.4.1.25 3.2.1.68	Sulfolobus solfataricus P2	AAK42273.1 CAA69504.1 CAC23738.1 NP_343483.1	P95868	2VNC[A,B] 2VR5[A,B] 2VUY[A,B]	11
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
pullulanase (PulA) (Pul13A)	3.2.1.41	Bacillus acidopullulyticus	AAX00707.1 ACK18363.1 CAC60156.1 CAX87014.1		2WAN[A]	14
α-amylase (Amy1;BAA)	3.2.1.1	Bacillus amyloliquefaciens	AAA22191.1 AAE12381.1 AAE13947.1 AAE37101.1 AAE59727.1 AAE62349.1 AAN20003.1 AAN27445.1 AAN99911.1 AAP02211.1 AAR63623.1 AAR76030.1 AAS26291.1 AAY72173.1 ABJ43095.1 ABJ43099.1 ABN29369.1 ACK07344.1 ACP75069.1 ACS10035.1 ACW26904.1 CAA01489.1 CAA23430.1 CAC16484.1 CAC88551.1 CAD19035.1 CAD20678.1 CAD26702.1 CAD67517.1 CAD88112.1	P00692.1	3BH4[A,B]	5
oligo-α-1,6-glucosidase (MalL)	3.2.1.10	Bacillus cereus ATCC 7064	CAA37583.1	P21332.1	1UOK[A]
β-cyclodextrin glucanotransferase (Cgt)	2.4.1.19	Bacillus circulans 251	CAA55023.1	P43379	1CDG[A] 1CGV[A] 1CGW[A] 1CGX[A] 1CGY[A] 1CXE[A] 1CXF[A] 1CXH[A] 1CXI[A] 1CXK[A] 1CXL[A] 1D3C[A] 1DTU[A] 1EO5[A] 1EO7[A] 1KCK[A] 1KCL[A] 1OT1[A] 1OT2[A] 1PEZ[A] 1PJ9[A] 1TCM[A,B] 2CXG[A] 2DIJ[A]	2
β-cyclodextrin glucanotransferase (CGTase)	2.4.1.19	Bacillus circulans 8	CAA48401.1	P30920.1	1CGT[A] 1CGU[A] 3CGT[A] 4CGT[A] 5CGT[A] 6CGT[A] 7CGT[A] 8CGT[A] 9CGT[A]	2
g-cyclodextrin glucanotransferase (Cgt)	2.4.1.19	Bacillus clarkii 7364	ABC06412.1 BAB91217.1 BAB91217.2 BAH14968.1 CAD87625.1	Q8L3E0	cryst	2
α-amylase	3.2.1.1	Bacillus halmapalus	AAE12377.1 AAE59724.1 AAE59730.1 AAE62346.1 AAE62352.1 AAP02208.1 AAP02214.1 AAS26288.1 AAS26294.1 AAY06017.1 AAY72170.1 AAY72176.1 ACK07341.1 ACP75066.1 ACS10037.1 ACW26901.1 CAC88548.1 CAD19032.1 CAD20675.1 CAD26699.1 CAD67514.1		1W9X[A] 2GJP[A] 2GJR[A]	5
α-amylase G-6 (BH0413)	3.2.1.98	Bacillus halodurans C-125	BAB04132.1 NP_241279.1	Q9KFR4	2C3G[A] 2C3H[A,B,C,D,E,F,G,H] 2C3V[A,B] 2C3W[A,B,C,D] 2C3X[A,B]	19
α-amylase (AmyA;AmyS;BLA)	3.2.1.1	Bacillus licheniformis	AAA22193.1 AAA22226.1 AAA22240.1 AAN99913.1 AAO26743.1 AAT46561.1 ACM17155.1 CAA01355.1 CAA03075.1 CAA26981.1 CAC16483.1 CAC88550.1 CAD19034.1 CAD20677.1 CAD26701.1 CAD26706.1 CAD88111.1 CAV31708.1	P06278.1 Q6GWE2	1BLI[A] 1BPL[A,B] 1OB0[A] 1VJS[A]	5
cyclomaltodextrinase (CDI5;BspCMD)	3.2.1.54	Bacillus sp.	AAA92925.1	Q59226	1EA9[C]	20
β-cyclodextrin glucanotransferase (Cgt)	2.4.1.19	Bacillus sp. 1011	AAA22308.1	P05618.1	1D7F[A] 1DED[A] 1I75[A] 1PAM[A] 1UKQ[A,B] 1UKS[A,B] 1UKT[A,B] 1V3J[A,B] 1V3K[A,B] 1V3L[A,B] 1V3M[A,B]	2
maltohexaose-forming amylase	3.2.1.98	Bacillus sp. 707	AAA22231.1 AAE59728.1 AAE62350.1 AAP02212.1 AAR63620.1 AAS26292.1 AAY06019.1 AAY72174.1 ACK07346.1 ACP75071.1 ACS10032.1 ACW26905.1	P19571.1	1WP6[A] 1WPC[A] 2D3L[A] 2D3N[A]	5
α-amylase (Kra2) (fragment)		Bacillus sp. KR8104	ACD93218.1 ACD93218.2		3DC0[A]	28
alkaline α-amylase (AmyK)	3.2.1.1	Bacillus sp. KSM-1378	AAN99906.1 AAR63625.1 AAR68734.1 AAV01045.1 ABE15847.1 ABI05897.1 ABY03536.1 ACS10038.1 ACW26917.1 BAA32431.1 CAD35985.1 CAD38135.1 CAV31709.1	O82839	2DIE[A]	5
α-amylase (AmyK38)	3.2.1.1	Bacillus sp. KSM-K38	AAN18958.1 AAV01046.1 ABA16539.1 ABI05896.1 ABY03537.1 ACS10039.1 BAB71820.1 CAC39917.1 CAD35986.1 CAD38133.1 CAJ00040.1 CAJ28078.1	Q93I48	1UD2[A] 1UD3[A] 1UD4[A] 1UD5[A] 1UD6[A] 1UD8[A]	5
α-amylase	3.2.1.1	Bacillus subtilis 2633 / NATTO	AAA22194.1 ABW34932.1 CAA30643.1	Q45516 Q45520	1BAG[A] 1UA7[A]	28
pullulanase type I / glycogen-debranching enzyme (AmyX;BSU29930)	2.4.1.25 3.2.1.33 3.2.1.41	Bacillus subtilis subsp. subtilis str. 168	AAC00283.1 ACH14984.1 CAB14971.1 CAB14971.2 NP_390871.1	O34587	2E8Y[A,B] 2E8Z[A,B] 2E9B[A,B]	14
sucrose phosphorylase (SucP)	2.4.1.7	Bifidobacterium adolescentis DSM 20083	AAO33821.1	Q84HQ2	1R7A[A,B] 2GDU[A,B] 2GDV[A,B]	18
malto-oligosyltrehalose trehalohydrolase (DR0464)	3.2.1.141	Deinococcus radiodurans R1	AAF10042.1 NP_294187.1	Q9RX51	2BHU[A] 2BHY[A] 2BHZ[A] 2BXY[A] 2BXZ[A] 2BY0[A] 2BY1[A] 2BY2[A] 2BY3[A]	10
branching-enzyme (GlgB;b3432)	2.4.1.18	Escherichia coli K-12 MG1655	AAA23872.1 AAA58230.1 AAC76457.1 ABX94236.1 CAP11381.1 CAV00219.1 NP_417890.1	P07762.1	1M7X[A]	9
pullulanase I (PulA)	3.2.1.41	Fervidobacterium pennivorans VEN5	AAD30387.1	Q9XDB5	cryst	14
cyclomaltodextrinase (Cdase;FspCMD)	3.2.1.54	Flavobacterium sp. 92	CAD32957.1	Q8KKG0	3EDD[A,B] 3EDE[A,B] 3EDF[A,B] 3EDJ[A,B] 3EDK[A,B]
α-glucosidase (Gsj)	3.2.1.20	Geobacillus sp. HTA-462	BAE48285.1	Q33E90	2ZE0[A]
maltogenic α-amylase (Novamyl)	3.2.1.133	Geobacillus stearothermophilus C599	AAA22233.1 AAE62848.1 AAE62849.1 AAY11155.1 ABA38666.1 CAL60144.1 CAZ67881.1	P19531.2	1QHO[A] 1QHP[A]	2
α-amylase (AmyS)	3.2.1.1	Geobacillus stearothermophilus DY5 / PHI300 / NZ-3	AAA22227.1 AAA22235.2 AAA22241.1 AAE59725.1 AAE62347.1 AAP02209.1 AAS26289.1 AAY06018.1 AAY72171.1 ACW26902.1 CAA26547.1	P06279.3	1HVX[A]	5
a/β-cyclodextrin glucanotransferase (Cgt)	2.4.1.19	Geobacillus stearothermophilus NO. 2	CAA41770.1 CAA41771.1 CAA41772.1	P31797.1	1CYG[A]	2
cyclomaltodextrinase / neopullulanase (NplT;BsCMD)	3.2.1.135 3.2.1.54	Geobacillus stearothermophilus TRS40	AAA22622.1	P38940	1J0H[A,B] 1J0I[A,B] 1J0J[A,B] 1J0K[A,B]	20
α-amylase A (AmyA)	3.2.1.1	Halothermothrix orenii	AAN52525.1 CAZ78883.1 CBF79188.1	Q8GPL8	1Q76 1WML 1WZA[A]	36
α-amylase B (AmyB)	3.2.1.1	Halothermothrix orenii	ACB11224.1	B2CCC1	3BC9[A] 3BCD[A] 3BCF[A]
pullulanase (PulA)	3.2.1.41	Klebsiella aerogenes W70	AAA25124.1 AAR52613.1	P07811.1	2FGZ[A] 2FH6[A] 2FH8[A] 2FHB[A] 2FHC[A] 2FHF[A]	13
isomaltulose synthase (PalI)	5.4.99.11	Klebsiella sp. LX3	AAK82938.1 CAF32992.1 CAF32993.1 CBF79192.1	Q8KR84	1M53[A]
α-amylase (Amy2;lp_0179)		Lactobacillus plantarum WCFS1	CAD62849.1 NP_784011.1		3DHU[A,B,C,D]
amylosucrase (Ams)	2.4.1.4	Neisseria polysaccharea ATCC 43768	AAT15258.1 AAW13710.1 ACK26675.1 CAA03058.1 CAA03083.1 CAA09772.1	Q9ZEU2	1G5A[A] 1JG9[A] 1JGI[A] 1MVY[A] 1MW0[A] 1MW1[A] 1MW2[A] 1MW3[A] 1S46[A] 1ZS2[A]	4
debranching enzyme (Npun_R6073)	2.4.1.25 3.2.1.33	Nostoc punctiforme PCC 73102 ATCC 29133	ACC84362.1 CAY02945.1 CAZ78799.1	B2IUW9	2WC7[A] 2WCS[A] 2WKG[A]	20
α-cyclodextrin glucanotransferase (CD;Cgt;CgtM)	2.4.1.19	Paenibacillus macerans IAM1243/ IB7 / IFO 3490 (NRRL B-388)	AAA22298.1 AAC04359.1 CAA41773.1	O52766 P04830.2	cryst	2
multidomain α-amylase / β-amylase	3.2.1.1 3.2.1.2	Paenibacillus polymyxa	AAA85446.1 CAA68344.1	P21543.1	cryst
sucrose isomerase (SmuA)	5.4.99.11	Protaminobacter rubrum CBS 547.77	AAE36427.1 AAY69568.1 AAY69572.1 ACW35802.1 ACW35805.1 CAF32985.1 CAF32988.1		3GBD[A] 3GBE[A]
α-amylase (Amy)	3.2.1.1	Pseudoalteromonas haloplanktis TAB23 / A23	CAA41481.1	P29957.3	1AQH[A] 1AQM[A] 1B0I[A] 1G94[A] 1G9H[A] 1JD7[A] 1JD9[A] 1KXH[A] 1L0P[A]
isoamylase (Iam;IsoA)	3.2.1.68	Pseudomonas amyloderamosa SB-15	AAA25854.1 CAA31754.1	P10342.3	1BF2[A]	11
sucrose isomerase / trehalulose synthase (MutB)	5.4.99.11	Pseudomonas mesoacidophila MX-45	AAY69574.1 ABC33903.1 ACO05018.1 CAF32995.1	Q2PS28	1ZJA[A,B] 1ZJB[A,B] 2PWD[A,B] 2PWE[A,B] 2PWF[A,B,C,D] 2PWG[A,B] 2PWH[A,B]
maltotetraose-forming amylase (AmyP)	3.2.1.60	Pseudomonas stutzeri MO-19	AAA25707.1 AAA25709.1 ABN31444.1 ABN31445.1 ABN31446.1 ABN31447.1 ABN31448.1 ABN31449.1 ABN31450.1 ABN31451.1 ABN31452.1	P13507.2	1AMG 1GCY[A] 1JDA[A] 1JDC[A] 1JDD[A] 1QI3[A] 1QI4[A] 1QI5[A] 1QPK[A] 2AMG[A]
pullulanase (Sap;SAG1216;SAG_1216)	3.2.1.41	Streptococcus agalactiae 2603V/R serotype V	AAN00098.1 NP_688225.1		3FAW 3FAX	12
pullulanase (SAN_1346)		Streptococcus agalactiae COH1	EAO76676.1	Q3DB05	3FAW[A] 3FAX[A]	12
dextran α-1,6-glucosidase (DexB)	3.2.1.70	Streptococcus mutans ATCC 25175	BAE79634.1	Q2HWU5	2ZIC[A] 2ZID[A]	31
α-amylase I (TVA I)	3.2.1.1	Thermoactinomyces vulgaris R-47	BAA02471.1 CAY02971.1 CAZ78902.1	Q60053	1JI1[A,B] 1UH2[A] 1UH3[A] 1UH4[A] 2D0F[A] 2D0G[A] 2D0H[A]
cyclomaltodextrinase / neopullulanase (TVA II;TvCMD)	3.2.1.135 3.2.1.54	Thermoactinomyces vulgaris R-47	BAA02473.1 BAA97040.1 BAB40638.1 CAY02972.1 CAZ78903.1	Q08751 Q7DI93	1BVZ[A,B] 1G1Y[A,B] 1JF5[A,B] 1JF6[A,B] 1JI2[A,B] 1JIB[A,B] 1JL8[A,B] 1VB9[A,B] 1VFK[A,B] 1VFM[A,B] 1VFO[A,B] 1VFU[A,B] 1WZK[A,B] 1WZL[A,B] 1WZM[A,B] 2D2O[A,B] 3A6O[A,B]	20
a/β-cyclodextrin glucanotransferase (AmyA)	2.4.1.19	Thermoanaerobacterium thermosulfurigenes EM1	AAB00845.1	P26827.2	1A47[A] 1CIU[A] 3BMV[A] 3BMW[A]	2
4-α-glucanotransferase (Mgt;TM0364)	2.4.1.25	Thermotoga maritima MSB8	AAD35451.1 CAA90693.1 NP_228175.1	P80099 Q60035	1LWH[A,B] 1LWJ[A,B]
4-α-glucanotransferase (MmtA)	2.4.1.25	Thermotoga maritima MSB8 (DSM 3109)	CAA04525.1	O33838	1GJU[A] 1GJW[A]
cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA)	3.2.1.133 3.2.1.54	Thermus sp. IM6501	AAC15072.1	O69007	1GVI[A] 1SMA[A]	20
TTHA1563		Thermus thermophilus HB8	BAD71386.1	Q5SI17	2Z1K[A,B,C,D]	20
sucrose hydrolase (Suh)	3.2.1.-	Xanthomonas axonopodis pv. glycines 8RA	AAQ93678.1	Q6UVM5	3CZE[A] 3CZG[A] 3CZK[A] 3CZL[A]	4
sucrose hydrolase (SuxB;XCC3359)	3.2.1.-	Xanthomonas campestris pv. campestris str. ATCC 33913	AAM42629.1 NP_638705.1		2WPG[A]	4
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
α-amylase	3.2.1.1	Aspergillus niger		P56271	2AAA[A]	1
α-amylase A / B (AmyA;AmyB;An05g02100)	3.2.1.1	Aspergillus niger CBS 513.88	CAK44871.1 CAL33800.1	A2QL05	2GUY[A] 2GVY[A,B]	1
α-amylase (AmyB;TAA;Amy1;Amy2;Amy3;Asp o 21;Aspo21)	3.2.1.1	Aspergillus oryzae DSM63303	AAA32708.1 ACP34134.1 BAA00336.1 CAA31218.1 CAA31219.1 CAA31220.1 CAC40348.1 CAT03377.1 CAV35984.1	P0C1B3 P10529	2TAA[A] 3KWX[A] 6TAA[A] 7TAA[A]	1
α-amylase (pancreatic) (Amy2A)	3.2.1.1	Homo sapiens	AAA51723.1 AAA51724.1 AAA51725.1 AAA52280.1 AAH07060.1 AAI46998.1 BAD97183.1 CAA30099.1 CAA30100.1 CAN87444.1 NP_000690.1	P04746 P04746.2	1B2Y[A] 1BSI[A] 1CPU[A] 1HNY[A] 1KB3[A] 1KBB[A] 1KBK[A] 1KGU[A] 1KGW[A] 1KGX[A] 1U2Y[A] 1U30[A] 1U33[A] 1XCW[A] 1XCX[A] 1XD0[A] 1XD1[A] 1XGZ[A] 1XH0[A] 1XH1[A] 1XH2[A] 2CPU[A] 2QMK[A] 2QV4[A] 3BAI[A] 3BAJ[A] 3BAK[A] 3BAW[A] 3BAX[A] 3BAY[A] 3CPU[A] 3IJ7[A] 3IJ8[A] 3IJ9[A]	24
α-amylase (salivary) (Amy1A;Amy1)	3.2.1.1	Homo sapiens	AAA16183.1 AAA52279.1 AAA58368.1 AAH63129.1 AAH69347.1 AAH69463.1 AAH70302.1 AAH92444.1 AAI32986.1 AAI32988.1 AAI32996.1 AAI32998.1 AAI44453.1 BAF85030.1 CAI14928.1 CAI14929.1 CAI14930.1 CAI14932.1 NP_004029.2	P04745.2	1C8Q[A] 1JXJ[A] 1JXK[A] 1MFU[A] 1MFV[A] 1NM9[A] 1Q4N[X] 1SMD[A] 1XV8[A,B] 1Z32[X] 3BLK[A] 3BLP[X] 3DHP[A]	24
4F2 heavy chain antigen (4f2hc)		Homo sapiens	AAA35489.1 AAA35536.1 AAA51540.1 AAA52497.1 AAH01061.1 AAH01061.2 AAH03000.1 AAH03000.2 AAS26022.1 AAS26189.1 ABA66456.1 ACC10874.1 ACJ91468.1 ACR85919.1 ACR85923.1 ACW43720.1 BAA84649.1 BAG65305.1 CAY39141.1 NP_002385.2 NP_002385.3	P08195.2	2DH2[A] 2DH3[A,B]	34
α-amylase 1 (Amy1;Amy1.1)	3.2.1.1	Hordeum vulgare	AAA32927.1 AAA32929.1	P00693.1 Q40017	1HT6[A] 1P6W[A] 1RP8[A] 1RP9[A] 1RPK[A] 2QPS[A] 2QPU[A,B,C] 3BSG[A] 3BSH[A]	6
α-amylase 2 (Amy2)	3.2.1.1	Hordeum vulgare	AAA32925.1 AAA98790.1 CAA33298.1	P04063.3 Q40015	1AMY[A] 1AVA[A,B] 1BG9[A]	6
starch-branching enzyme 1 (Sbe1;Rbe1;BEI;Os06g0726400)	2.4.1.18	Oryza sativa Japonica Group	AAD28284.1 AAP68993.1 AAU99032.1 AAX83629.1 ABA16988.1 ABD15942.1 ABD15943.1 ABD15944.1 ABD15945.1 ABE12599.1 ABL74557.1 ABL74558.1 ACM95329.1 ACY56114.1 BAA01584.1 BAA01616.1 BAA01855.1 BAD61712.1 BAD61713.1 BAD61804.1 BAD61805.1 BAF20543.1 BAG89369.1	Q01401.2 Q0D9D0 Q7XZP9	cryst	8
oligo-α-1,6-glucosidase / isomaltase (Fsp2;YGR287c)	3.2.1.10	Saccharomyces cerevisiae S288C	BAA07818.1 CAA97319.1 CAA97321.1 CBF79247.1 NP_011803.1	P53051	cryst
α-amylase (Ppa) (pancreatic)	3.2.1.1	Sus scrofa	AAF02828.1	P00690.3	1BVN[P] 1DHK[A] 1HX0[A] 1JFH[A] 1KXQ[A] 1KXT[A] 1KXV[A] 1OSE[A] 1PIF[A] 1PIG[A] 1PPI[A] 1UA3[A] 1VAH[A] 1WO2[A]	24
α-amylase	3.2.1.1	Tenebrio molitor		P56634	1CLV[A] 1JAE[A] 1TMQ[A] 1VIW[A]	15