Glycoside Hydrolase Family 5

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); β-glycosidase (EC 3.2.1.-); endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); β-N-acetylhexosaminidase (EC 3.2.1.52); chitosanase (EC 3.2.1.132); β-D-galactofuranosidase (EC 3.2.1.146); β-galactosylceramidase (EC 3.2.1.46); ; β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-); α-L-arabinofuranosidase (EC 3.2.1.55); glucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (22978); Uniprot accession (634); PDB accession (263); 3D entries (100); cryst (0)
| 1 | 2 | 3 | 4 | 5 | 6 |
Archaea
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 cellulase (EBI-244) 3.2.1.4 pubmed
Desulfurococcaceae archaeon EBI-244 AEB53062.1 F6M085  
 cellulase / cellobiohydrolase (Hu-CBH1;Huta_2387) 3.2.1.- doi
Halorhabdus utahensis DSM 12940 ACV12553.1 C7NVN0  
 endo-β-1,4-glucanase (EglB;TCel4;PAB0632) 3.2.1.4 pubmed
Pyrococcus abyssi GE5 CAB49854.1
AEG76944.1
NP_126623.1
Q9V052   1
 endo-β-1,4-glucanase (EGPh;EglB;TCel3;PH1171) 3.2.1.4 pubmed
Pyrococcus horikoshii OT3 AAQ31833.1
AAQ31832.1
BAA30271.1
NP_143072.1
O58925 2ZUM[A]
2ZUN[A,B,C]
3AXX[A,B,C]
3QHM[A,B,C]
3QHN[A,B,C]
3QHO[A,B,C]
3VVG[A,B,C]
3W6L[A,B,C]
3W6M[A,B,C]
4DM1[A,B,C]
4DM2[A,B,C]
1
 thermoactive glycoside hydrolase family 5 endoglucanase (Cel5A) 3.2.1.4 pubmed
uncultured archaeon AZP54316.1 A0A3Q9CZQ1   1
 exomannanase 3.2.1.100 pubmed
uncultured archaeon QFQ13828.1 A0A5P8EA52   19
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 glucomannanase (AceceDRAFT_0142) 3.2.1.- pubmed
Acetivibrio cellulolyticus WP_010248927.1 A0A5S8WF61 6MQ4[A] 4
 AcGH5 / AceceDRAFT_1592 3.2.1.- pubmed
Acetivibrio cellulolyticus CD2 EFL62381.1 E1KC96   34
 endo-β-1,4-glucanase E (CelE;EgE) (Cel5C) 3.2.1.4
3.2.1.8
3.2.1.-
pubmed
pubmed
pubmed
Acetivibrio thermocellus AAA23224.1 P10477 4IM4[A,B,C,D,E,F] 4
 Cthe_0405 (CelL) 3.2.1.4 pubmed
Acetivibrio thermocellus ATCC 27405 ABN51643.1 A3DCG4   1
 endo-β-1,4-glucanase B (CelB;EgB;Cthe_0536) (Cel5A) 3.2.1.4 pubmed
Acetivibrio thermocellus ATCC 27405 ABN51772.1 P04956   1
 β-mannanase (CtMan5A;Cthe_0821) (Man5A) 3.2.1.78 pubmed
Acetivibrio thermocellus ATCC 27405 ABN52056.1 A3DDM7   55
 bifunctional endo-β-1,4-glucanase / β-1,4-xylanase H (CelH;EgH;CtCelH;CtCel5E;Cthe_1472) (Lic26A;Cel5E) 3.2.1.4
3.2.1.8
pubmed
pubmed
Acetivibrio thermocellus ATCC 27405 ABN52701.1
CCF82162.1
H6SHY4
P16218
4U3A[A,B]
4U5I[A,B]
4U5K[A,B]
5BYW[A,B,C,D,E]
25
 xylanase (M6;CtM6;CtXyl5A;Cthe_2193) (arabinoxylan-specific) (Xyl5A) 3.2.1.- pubmed
Acetivibrio thermocellus ATCC 27405 ABN53395.1 A3DHG6 2Y8K[A]
5AK1[A]
5G56[A]
5LA0[A]
5LA1[A]
5LA2[A,B]
34
 endo-β-1,4-glucanase G (CelG;Cthe_2872) (Cel5D) 3.2.1.4 pubmed
Acetivibrio thermocellus ATCC 27405 ABN54070.1 Q05332   1
 cellobiohydrolase (CelO) (Cel5F) 3.2.1.74 pubmed
Acetivibrio thermocellus F7 CAB76938.1 Q9L3J2   1
 endo-β-1,4-glucanase C (CelC) 3.2.1.4 doi
Acetivibrio thermocellus F7 CAC27410.1 Q9AL36   37
 endo-β-1,4-glucanase B (CelB;EgB) (Cel5A) 3.2.1.4 pubmed
Acetivibrio thermocellus NCIB 10682 CAA27266.1 P04956   1
 endo-β-1,4-glucanase G (CelG) (Cel5D) 3.2.1.4 pubmed
Acetivibrio thermocellus NCIB 10682 CAA49187.1 Q05332   1
 endo-β-1,4-glucanase / β-1,3:1,4-glucanase H (CelH) (Lic26A;Cel5E) 3.2.1.4 pubmed
Acetivibrio thermocellus NCIB 10682 AAA23225.1 H6SHY4
P16218
  25
 endo-β-1,4-glucanase C (LC2;CelC;EgC) (Cel5B) 3.2.1.4 pubmed
Acetivibrio thermocellus NCIB 10682EL AAA23220.1 P07985
P0C2S3
1CEC[A]
1CEN[A]
1CEO[A]
37
 endo-β-1,4-glucanase E1 (EGAc;EGI;TcE1;Acel_0614) (Cel5A) 3.2.1.4 pubmed
Acidothermus cellulolyticus 11B AAA75477.1
ABH84883.1
ABK52387.1
ACW33392.1
CAD42489.1
CAR96693.1
P54583 1C0D[A,B]
1ECE[A,B]
1VRX[A,B]
1
 endo-β-1,4-glucanase 3.2.1.4 pubmed
Actinomyces sp. 40 AAC06196.1 O66064   2
 endo-β-1,4-glucanase (Egl-1139) / III-3A (Cel5A) 3.2.1.4 pubmed
Alkalihalobacillus akibai / KSM-1139 BAA00045.1
AAA22305.1
ACE14924.1
P06564   2
 endoglucanase (EG-I-1) 3.2.1.4 pubmed
Alkalihalobacillus akibai I-1 AFI25187.1 I1V1X7   2
 β-glucanase (CelB;BhCel5B;BH0603) (Cel5B) 3.2.1.4 pubmed
Alkalihalobacillus halodurans C-125 BAB04322.1
NP_241469.1
Q9KF82 4V2X[A] 4
 endoglucanase (Egl;EglA;azo2236) 3.2.1.4 pubmed
Azoarcus sp. BH72 ABB92850.1
CAL94853.1
A0S6A6   5
 endo-β-1,4-glucanase (Egl;AZC_1301) 3.2.1.4 pubmed
Azorhizobium caulinodans ORS 571 BAF87299.1 A8I0V5   1
 β-glucosidase (Avin_07130) 3.2.1.21 pubmed
Azotobacter vinelandii DJ ATCC BAA-1303 ACO76963.1 C1DLL5   45
 endoglucanase (EglII) 3.2.1.4 doi
Bacillus amyloliquefaciens PSM 3.1 ADC54852.1 D3Y2F0   2
 endoglucanase A (EngA) 3.2.1.4 pubmed
Bacillus amyloliquefaciens UMAS1002 AAL99668.1 Q8RPQ6   2
 endo-β-1,4-glucanase (Egl01) 3.2.1.4 pubmed
Bacillus licheniformis AIY72753.1 A0A0A7EZH7   2
 endoglucanase (BlCel5B;BLi01882;BL01230) (Cel5B) 3.2.1.4 pubmed
Bacillus licheniformis DSM 13 = ATCC 14580 AAU40777.1
AAU23417.1
Q65JI7 4YZP[A]
4YZT[A]
4
 endo-β-1,4-glucanase C (BglC;BLi02088;BL01471) (Cel5A) 3.2.1.4 doi
Bacillus licheniformis DSM 13 = ATCC 14580 AAU23613.2
AAU23613.1
AAU40977.1
ACR87895.1
CAF05574.1
CAV28462.1
A5A671
Q65IY7
  2
 endo-β-1,4-glucanase (Cel5A) (Cel5A) 3.2.1.4 doi
Bacillus licheniformis GXN151 / B-41361 AAP51020.1 Q7X3S6   2
 endo-β-1,4-glucanase 3.2.1.4 pubmed
Bacillus sp. (in: Bacteria) 22-28 BAA12744.1 Q45554   2
 endo-β-1,4-glucanase 3.2.1.4 doi
Bacillus sp. (in: Bacteria) 5H BAA31712.1 O83012   2
 endo-β-1,4-glucanase B1 3.2.1.4 pubmed
Bacillus sp. (in: Bacteria) N186-1 CAA83942.1 Q59232   2
 CelS 3.2.1.4 doi
Bacillus sp. 79-23 AAC02536.1 O52731   2
 cellulase (CelB) 3.2.1.4 pubmed
Bacillus sp. BG-CS10 ADD62401.1 D4P8C6 5E09[A]
5E0C[A]
5XRC[A,B,C]
4
 β-mannanase (Amn5) (Man5A) 3.2.1.78 doi
Bacillus sp. JAMB-602 BAD99527.1 Q4W8M3 1WKY[A] 8
 endo-β-1,4-glucanase K (Egl-K) 3.2.1.4 pubmed
Bacillus sp. KSM-635 AAA22304.1 P19424 1G01[A]
1G0C[A]
2
 endo-β-1,4-glucanase (Egl-64) 3.2.1.4 pubmed
Bacillus sp. KSM-64 / Bacillus sp. Z-16 CAJ00039.1
AAA73189.1
ACE14925.1
ACW22976.1
CAJ28077.1
CAT16610.1
Q59241   2
 endoglucanase N252 (Egl252) 3.2.1.4 pubmed
Bacillus sp. KSM-N252 BAB62295.1 Q93R81   2
 endo-β-1,4-glucanase (Egl-237) 3.2.1.4 pubmed
Bacillus sp. KSM-S237 CAJ00038.1
ABH71811.1
ACE14923.1
ACW22975.1
BAB19360.1
CAF02232.1
CAJ28076.1
CAT16607.1
Q9F216   2
 β-mannanase (ManA;MAN330) (Man5A) 3.2.1.78 doi
Bacillus sp. N16-5 AAT06599.1
AML27065.1
Q5YEX6 3JUG[A] 8
 bifunctional cellulase chitosanase (CelA) 3.2.1.132
3.2.1.4
URL
URL
Bacillus sp. NBL420 AAK73277.1 Q93LI9   2
 endo-β-1,4-glucanase 3.2.1.4 pubmed
Bacillus subtilis AAA22307.1 Q45532   2
 β-1,3-1,4-glucanase / cellulase C (CelA;CelC) 3.2.1.4 pubmed
Bacillus subtilis A8-8 AAO63626.1 Q6Y4Q1   2
 endoglucanase (Egl173;Cel;EglS) 3.2.1.4 doi
Bacillus subtilis BEC-1 / Y106 / shu-3 AAK39540.1
ADM12805.1
ADP05286.1
Q93TJ6   2
 endo-β-1,4-glucanase (Eg15) (Cel5A) 3.2.1.4 pubmed
Bacillus subtilis BME-15 ACI15227.1
ACJ12786.1
ACJ12787.1
ACJ12788.1
ACJ12789.1
ACJ12790.1
ACJ12791.1
ACJ12792.1
B6DVJ2
B6VEU9
B6VEV0
B6VEV1
B6VEV2
B6VEV3
B6VEV4
B6VEV5
  2
 endo-β-1,4-glucanase (CMCase;BglC) 3.2.1.4 pubmed
Bacillus subtilis BSE616 BAA00859.1 P23549   2
 endo-β-1,4-glucanase (CelG) 3.2.1.4 URL
Bacillus subtilis CHZ1 AAK94871.1 Q93LD0   2
 endo-β-1,4-glucanase (EglS) 3.2.1.4 pubmed
Bacillus subtilis CK-2 CAA47429.1 G1DE47
P10475
  2
 endo-β-1,4-glucanase (BglC;Gld) 3.2.1.4 pubmed
Bacillus subtilis DLG AAA22496.1 P07983   2
 cellulase (CelDR) 3.2.1.4 pubmed
Bacillus subtilis DR ABV45393.1
ACI63223.1
A8D0T0
B6V3I2
  2
 endo-β-1,4-glucanase (CelI15) 3.2.1.4 pubmed
Bacillus subtilis I15 ACK38261.1 B7UAM4   2
 endo-β-1,4-glucanase 3.2.1.4 pubmed
Bacillus subtilis IARI-SP-1 AGW99977.1 U3RAX4   2
 endo-β-1,4-glucanase (Egl499;Egl330) 3.2.1.4 doi
Bacillus subtilis JA18 AAZ22322.1 Q45T78   2
 cellulase (Cel15;Cell15) 3.2.1.4 pubmed
Bacillus subtilis LN ACR59602.1 C5I946   2
 cellulase (EG) 3.2.1.4 doi
Bacillus subtilis NC1 / WRL101 BAL46915.1 H3K351   2
 endo-β-1,4-glucanase (EglS;BglC;Gld;BSU18130;BsCel5;BsCel5A) (Cel5A) 3.2.1.4 pubmed
Bacillus subtilis subsp. subtilis str. 168 CAA82317.1
AEJ76923.1
AIY93123.1
AQR81804.1
AQR86018.1
CAA97610.1
CAB13696.2
NP_389695.1
A0A6M3ZBL5
G1DE47
P10475
3PZT[A,B]
3PZU[A,B]
3PZV[A,B,C,D]
2
 endo-β-1,4-glucanase 3.2.1.4 doi
Bacillus subtilis subsp. subtilis UMC7 AGG11030.1 M1R5G7   2
 cellulase (CelA10) 3.2.1.4 doi
bacterium enrichment culture clone CelA10 ACR23656.1 C5HG53   2
 cellulase (CelA24) 3.2.1.4 doi
bacterium enrichment culture clone CelA24 ACR23659.1 C5HG56   26
 endoxylanase (ORF1299;BACEGG_01299) (BeXyn5A) 3.2.1.8 pubmed
Bacteroides eggerthii DSM 20697 EEC54456.1 B7AFX5   21
 endo-β-1,4-xylanase (BiXyn5A;ORF4213;BACINT_04213) (Xyn5A) 3.2.1.8 pubmed
Bacteroides intestinalis DSM 17393 EDV05070.1 B3CET2   21
 endo-xyloglucanase (CelA;BoGH5A;BACOVA_02653;Bovatus_03072) 3.2.1.151 pubmed
Bacteroides ovatus ATCC 8483 ALJ47680.1 A7LXT7 3ZMR[A,B] 4
 β-D-galactofuranosidase (Bovatus_02440; revised model of BACOVA_02949 ZP_02065960.1) 3.2.1.146 pubmed
Bacteroides ovatus ATCC 8483 ALJ47066.1     13
 endo-cellulase (AC2aCel5A) (Cel5A) 3.2.1.4 pubmed
Bacteroidetes bacterium AC2a AIJ19564.1 A0A076MPD7 4YHE[A,B] 4
 Balac_1450 3.2.1.78 pubmed
Bifidobacterium animalis subsp. lactis Bl-04 ACS46797.1 C6A9J9   8
 β-mannosidase (BL1333; BlMan5B) (BlMan5B) 3.2.1.25 pubmed
Bifidobacterium longum NCC2705 AAN25133.1
NP_696497.1
Q8G4P5   18
 β-1,4-mannosidase (HMPREF0175_1989) 3.2.1.25 pubmed
Bifidobacterium longum subsp. longum ATCC 55813 EEI79871.1 C2GY91 B[nd]
C[nd]
D[nd]
7LR1[A]
7LR2[A]
7LR6[A]
18
 endo-1,4-glucanase A 3.2.1.4 pubmed
Butyrivibrio fibrisolvens A46 AAA20893.1 P22541   2
 endo-β-1,4-glucanase 1 (End1;EglI;EGI) 3.2.1.4 pubmed
Butyrivibrio fibrisolvens H17C CAA35574.1 P20847   4
 endo-β-1,4-glucanase (TTE0359) (Cel5A) 3.2.1.4 pubmed
Caldanaerobacter subterraneus subsp. tengcongensis MB4 AAM23649.1
NP_622045.1
Q8RCQ7   2
 endomannanase / endoglucanase (ManB) (Man5B) 3.2.1.78 pubmed
Caldanaerobius polysaccharolyticus KMTHCJ AGA35556.1
ADK22147.1
D9J0D7 3W0K[A,B] 36
 β-mannanase A (ManA;CelA) (Man5A) 3.2.1.78 pubmed
Caldanaerobius polysaccharolyticus KMTHCJ AAD09354.1 Q9ZA17  
 β-1,4-mannanase (ManA) 3.2.1.78 pubmed
Caldibacillus cellulovorans AAF22274.1 Q9RFX5   8
 endo-β-1,4-glucanase D (CelD; Athe_0594) 3.2.1.4 pubmed
Caldicellulosiruptor bescii DSM 6725 CAB01405.2
ACM59720.1
CAB01405.1
CAB01405.2
B9MPF9
Q59154
  2
 bifunctional mannanase / endoglucanase (CbMan5A/Cel44A;Athe_1859;Cbes_1859;ORF1946) (Man5A-Cel44A) 3.2.1.78 pubmed
Caldicellulosiruptor bescii DSM 6725 ACM60947.1 B9MKT9   8
 bifunctional cellulase / β-mannanase (CelC-ManB;CbCel9B/Man5A;Athe_1865;Cbes_1865;Orf1952) (Cel9B-Man5A) 3.2.1.78 pubmed
Caldicellulosiruptor bescii DSM 6725 ACM60953.1 B9MKU5   8
 bifunctional β-mannanase / endo-β-1,4-glucanase (ManA) 3.2.1.78 pubmed
Caldicellulosiruptor saccharolyticus AAA71887.1
AAA72861.1
P22533   8
 endo-β-1,4-glucanase / exo-β-1,4-glucanase (CelB;EgB) 3.2.1.4 pubmed
Caldicellulosiruptor saccharolyticus CAA31936.1
A43802
P10474   1
 endoglucanase (Csac_0678) 3.2.1.4 pubmed
Caldicellulosiruptor saccharolyticus DSM 8903 ABP66297.1 A4XHB2 5ECU[A] 2
 cellulase (CBH-B;CelB;Csac_1078) 3.2.1.4 pubmed
Caldicellulosiruptor saccharolyticus DSM 8903 ABP66692.1 A4XIF7   1
 endo-β-1,4-glucanase (CelB) 3.2.1.4 pubmed
Caldicellulosiruptor sp. F32 AFO70071.1 I7CBM8   2
 multidomain endoglucanase (CelB) 3.2.1.4 pubmed
Caldicellulosiruptor sp. Tok7B.1 AAD30364.1 Q9X3P6   1
 β-mannosidase (Caci_2257) 3.2.1.25 pubmed
Catenulispora acidiphila DSM 44928 ACU71175.1 C7QJF2   18
 Caul_1283 (Cel5A) 3.2.1.4 pubmed
Caulobacter sp. K31 ABZ70413.1 B0SYT2   25
 endo-β-1,4-glucanase D (CenD;Celf_1924) (Cel5A) 3.2.1.4 pubmed
Cellulomonas fimi ATCC 484 AAA23089.1
AEE46054.1
F4GZL0
P50400
  1
 endo-β-1,4-glucanase B (CelB;CflB;CelCflB;SP18) 3.2.1.4 pubmed
Cellulomonas flavigena CDBB-531 AAD48494.3
AAD48494.2
Q9S3V3   1
 endoglucanase (CelA) 3.2.1.4 pubmed
Cellulophaga sp. QY3 ADB80152.1 K9J9W6   2
 endoglucanase / xylanase (CGSCsYakCAS_09989) (Cel5A) 3.2.1.- pubmed
Cellulosilyticum ruminicola CGMCC 1.5065 ACZ98591.1 D2KFI9   4
 β-1,4-mannanase (ManH) 3.2.1.78 pubmed
Cellulosimicrobium sp. HY-13 AEE43708.1 G3FBL1   8
 endo-β-1,4-mannanase 5A (CjMan5A;CJA_3338) (Man5A) 3.2.1.78 pubmed
Cellvibrio japonicus Ueda107 ACE84673.1
AAO31759.1
B3PF24
Q840C2
  8
 endo-β-1,4-glucanase E / 5B (CelE;CJA_2983) (Cel5B) 3.2.1.4 pubmed
Cellvibrio japonicus Ueda107 ACE84076.1
CAA60493.1
B3PCS3
Q59665
  2
 endo-β-1,4-mannanase 5C (CjMan5C;CJA_3470) (Man5C) 3.2.1.78 pubmed
Cellvibrio japonicus Ueda107 ACE82655.1
AAO31761.1
B3PG16
Q840C0
  7
 cellodextrinase C / 5A (CelC;CJA_1462) (Cel5A) 3.2.1.74 pubmed
Cellvibrio japonicus Ueda107 ACE82870.1
CAA43597.1
B3PDK2
P27033
  53
 endo-β-1,4-mannanase 5B (CJA_2475) (Man5B) 3.2.1.78 pubmed
Cellvibrio japonicus Ueda107 AAO31760.1
ACE84941.1
B3PKK7
Q840C1
  8
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