Glycoside Hydrolase Family 5

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); β-glycosidase (EC 3.2.1.-); endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); β-N-acetylhexosaminidase (EC 3.2.1.52); chitosanase (EC 3.2.1.132); β-D-galactofuranosidase (EC 3.2.1.146); β-galactosylceramidase (EC 3.2.1.46); ; β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-); α-L-arabinofuranosidase (EC 3.2.1.55); glucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (24948); Uniprot accession (634); PDB accession (270); 3D entries (100); cryst (0)
| 1 | 2 | 3 | 4 | 5 | 6 |
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 endo-β-1,4-mannanase 5C (CjMan5C;CJA_3470) (Man5C) 3.2.1.78 pubmed
Cellvibrio japonicus Ueda107 ACE82655.1
AAO31761.1
B3PG16
Q840C0
  7
 cellodextrinase C / 5A (CelC;CJA_1462) (Cel5A) 3.2.1.74 pubmed
Cellvibrio japonicus Ueda107 ACE82870.1
CAA43597.1
B3PDK2
P27033
  53
 endo-xyloglucanase (CjGH5E; CJA_3337) (Cel5E) 3.2.1.151 pubmed
Cellvibrio japonicus Ueda107 ACE83841.1     4
 endo-xyloglucanase (CjGH5D; CJA_3010) (Cel5D) 3.2.1.151 pubmed
Cellvibrio japonicus Ueda107 ACE84905.1   5OYC[A,B]
5OYD[A,B]
5OYE[A,B]
6HA9[A,B]
6HAA[A,B]
4
 endo-β-1,4-mannanase 5B (CJA_2475) (Man5B) 3.2.1.78 pubmed
Cellvibrio japonicus Ueda107 AAO31760.1
ACE84941.1
B3PKK7
Q840C1
  8
 endo-xyloglucanase (CjGH5F; CJA_2959) (Cel5F) 3.2.1.151 pubmed
Cellvibrio japonicus Ueda107 ACE86198.1     4
 endo-β-1,4-glucanase A (CelA) (Cel5A) 3.2.1.4 pubmed
Cellvibrio mixtus ATCC 12120 AAB61461.1 O07652   26
 endo-β-1,4-glucanase B (CelB) (Cel5A;Cel5B) 3.2.1.4 pubmed
Cellvibrio mixtus ATCC 12120 AAB61462.2 O07653   2
 β-mannosidase 5A (Man5A) (Man5A) 3.2.1.25 pubmed
Cellvibrio mixtus NCIMB 8633 AAS19695.1 Q6QT42 1UUQ[A]
1UZ4[A]
7ODJ[A]
7
 cellulase (CelA;pCS0061) 3.2.1.4 doi
Clavibacter michiganensis subsp. sepedonicus ATCC33113 AAK16222.1
CAQ03244.1
B0RJ54
Q9AF65
  1
 endo-/exo-type cellulase (CelEdx16) 3.2.1.4 pubmed
Clostridiaceae bacterium AN-C16-KBRB ADK66823.1 E0A3Q5   4
 CDIF28668_02723 3.2.1.4 doi
Clostridioides difficile DSM 28668 AXU72614.1 A0A5Q9L120 6UJE[A]
6UJF[A]
25
 glucomannanase (CAC0826) 3.2.1.151
3.2.1.-
pubmed
pubmed
Clostridium acetobutylicum ATCC 824 AAK78802.1
NP_347462.1
Q97KU0 6PZ7[A] 4
 endo-β-1,4-glucanase 5A (EngF) (Cel5A) 3.2.1.4 pubmed
Clostridium cellulovorans AAB40891.1 P94622   2
 endo-β-1,4-glucanase / xylanase B (EngB;Clocel_1150) 3.2.1.4
3.2.1.8
pubmed
pubmed
Clostridium cellulovorans 743B AAA23231.1
ADL50906.1
C5RHC3
P28621
  4
 endoglucanase G (EngG;Clocel_2600) 3.2.1.4 pubmed
Clostridium cellulovorans 743B ADL52309.1 D9SQV3   55
 β-mannanase A (ManA;Clocel_2818) 3.2.1.78 pubmed
Clostridium cellulovorans 743B AAF06110.2
ADL52514.1
C5RQ36
D9SS67
Q9LAJ3
  17
 endo-β-1,4-glucanase / xylanase D (EngD) 3.2.1.4
3.2.1.8
pubmed
pubmed
Clostridium cellulovorans ATCC 35269 AAA23233.1 P28623 3ICG[A,B,C,D]
3NDY[A,B,C,D]
3NDZ[A,B,C,D]
4
 endo-β-1,4-glucanase E / dual cellulase-mannanase (EngE) 3.2.1.4
3.2.1.-
pubmed
pubmed
Clostridium cellulovorans ATCC 35269 AAD39739.1 D9SV64
Q9XD99
6UI3[A] 4
 endo-β-1,4-glucanase (CelA) 3.2.1.4 pubmed
Clostridium longisporum AAC37035.1 P54937 6Q1I[A,B] 4
 endo-β-1,4-glucanase (EglA) 3.2.1.4 pubmed
Clostridium saccharobutylicum P262 AAA23230.1 P15704   2
 endo-β-1,4-glucanase C307 (CelC307) 3.2.1.4 pubmed
Clostridium sp. F1 BAA00793.1 P23340   37
 exo-β-1,4-mannosidase (CaMan5_18; PAZ_c15370) 3.2.1.25 pubmed
Cutibacterium acnes 266 AEE72695.1 A0A2B7I7A6 6GVB[A,B,C,D] 18
 endoglucanase 5A (ChCel5A; CHU_1107) (Cel5A) 3.2.1.4 pubmed
Cytophaga hutchinsonii ATCC 33406 ABG58383.1 A0A6N4SQ01
Q11W33
  2
 periplasmic endoglucanase 5B (CHU_2103) (Cel5B) 3.2.1.4 pubmed
pubmed
Cytophaga hutchinsonii ATCC 33406 ABG59366.1 A0A6N4ST27
Q11T98
5IHS[A] 2
 endo-β-1,4-glucanase (Cel5Z) 3.2.1.4 pubmed
Dickeya chrysanthemi PY35 AAF18152.1 Q9REW0   2
 endo-β-1,4-glucanase Z (CelZ;EgZ;Dda3937_02793) (Cel5A=Cel5Z) 3.2.1.4 pubmed
Dickeya dadantii 3937 ADM99099.1
CAA68604.1
P07103 1EGZ[A,B,C] 2
 DICTH_0008 (Cel5A) 3.2.1.4 pubmed
Dictyoglomus thermophilum H-6-12 ACI18520.1 B5YAS2   25
 endoglucanase (LC1;CelA;Dtur_0670) (Cel5B) 3.2.1.4 pubmed
Dictyoglomus turgidum DSM 6724 ACK41955.1 B8DZM2   25
 endo-β-1,4-mannanase (Dtur_0671) 3.2.1.78 pubmed
Dictyoglomus turgidum DSM 6724 ACK41956.1 B8DZM3   36
 endo-xyloglucanase (DgGH5; HMPREF9455_00295) 3.2.1.151 pubmed
Dysgonomonas gadei ATCC BAA-286 EGJ99262.1     4
 β-mannanase (Man1E ; M942_06180) 3.2.1.78 pubmed
Enterobacter cloacae P101 AHE72721.1     41
 endoglucanase (EcCel5A) (Cel5A) 3.2.1.4 pubmed
pubmed
Eubacterium cellulosolvens 5 BAE46390.1 Q3LHN3   4
 endoglucanase (EcCel5A) (Cel5A) 3.2.1.4 pubmed
pubmed
Eubacterium cellulosolvens 5 BAE46390.1 Q3LHN3   4
 β-mannanase (EsGH5_8; EUS_11000) 3.2.1.78 pubmed
[Eubacterium] siraeum 70/3 CBK96294.1     8
 endo-β-1,4-glucanase A (CelA;EgA;Bcell_0438) 3.2.1.4 pubmed
Evansella cellulosilytica DSM 2522 ADU28720.1
AAA22301.1
D2LVP1
E6TWN7
P06566
  2
 endo-β-1,4-glucanase B (CelB;EgB;Bcell_0437) 3.2.1.4 pubmed
Evansella cellulosilytica DSM 2522 ADU28719.1
AAA22299.1
D2LVP0
E6TWN6
P06565
  2
 endo-β-1,4-glucanase C (CelC;EgC;Cel2;Bcell_3370) 3.2.1.4 pubmed
Evansella cellulosilytica DSM 2522 ADU31612.1
AAA22306.1
D2LQZ9
E6U1W5
P19570
  2
 endo-β-1,4-glucanase (FnCel5A;Fnod_1560) (Cel5A) 3.2.1.4 pubmed
Fervidobacterium nodosum Rt17-B1 ABS61403.1
ADD73709.1
A7HNC0
D4PEB3
3NCO[A,B]
3RJX[A]
3RJY[A]
25
 Fisuc_3081 / FSU_0347 3.2.1.4 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 ACX76661.1
ADL25927.1
    4
 endo-β-1,4-glucanase 3 (Cel3;Cel-3;Eg3;Fisuc_2230;FSU_2772) (Cel5G) 3.2.1.4 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 AAA24893.1
ACX75816.1
ADL25000.1
P14250  
 endo-β-1,4-glucanase G (CelG;Fisuc_0897;FSU_1346) 3.2.1.4 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 AAB38548.1
ACX74504.1
ADL25356.1
C9RNQ8
Q47916
  4
 cellodextrinase A (CedA;Fisuc_1584;FSU_2070) (Cel5C) 3.2.1.74 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 AAA50210.1
ACX75179.1
ADL26975.1
C9RRJ3
D9SBT4
Q59441
  52
 endoglucanase (partial) 3.2.1.4 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 AAC06197.1 O66065   2
 cellulase (Fisuc_2364;FSU_2914) (Cel5H) 3.2.1.4 pubmed
Fibrobacter succinogenes subsp. succinogenes S85 ABU45500.1
ACX75950.1
ADL27061.1
A7UG69   2
 endo-β-1,4-glucanase (CelA;GsCelA) 3.2.1.4 pubmed
Geobacillus sp. 70PC53 ACJ60856.1 C5H6X3 4XZB[A]
4XZW[A]
2
 β-mannanase (ManF) 3.2.1.78 pubmed
Geobacillus stearothermophilus MCA2184 AAC71692.1 Q9ZI87   7
 β-xylosidase (GE39) 3.2.1.37 pubmed
Geobacillus thermodenitrificans T81 ATG84609.1     22
 endoglucanase (GE40) (fragment) 3.2.1.4 pubmed
Geobacillus thermodenitrificans T81 ATG84593.1     4
 endoglucanase (Cel5;HCH_03914) 3.2.1.4 pubmed
Hahella chejuensis KCTC 2396 ABC30636.1 Q2SFD8   2
 β-glucanase (CelB; BhCel5B; BH0603) (Cel5B) 3.2.1.4 pubmed
Halalkalibacterium halodurans C-125 BAB04322.1
NP_241469.1
Q9KF82 4V2X[A] 4
 endo-β-1,4-glucanase 5A (CpCel5A;Cphy_1163) (Cel5A) 3.2.1.4 pubmed
Lachnoclostridium phytofermentans ISDg ABX41541.1 A9KN42   1
 endo-β-1,4-glucanase (CpCel5C;Cphy_2058) (Cel5C) 3.2.1.4 doi
Lachnoclostridium phytofermentans ISDg ABX42426.1 A9KIH4   4
 endoglucanase (CelA;lpg1918) 3.2.1.4 pubmed
Legionella pneumophila subsp. pneumophila str. Philadelphia 1 AAU27988.1
AGH53362.1
Q5ZU89  
 β-galactosidase (BgLm1; LEUM_0847) 3.2.1.23 doi
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 ABJ61955.1     44
 endoglucanase (Cel5D) 3.2.1.4 pubmed
Martelella mediterranea WP_018063499.1     5
 endo-β-1,4-glucanase (MaCel5A) (Cel5A) 3.2.1.4 doi
Microbulbifer sp. ALW1 QSR83793.1     2
 endo-β-1,4-mannanase (GA0070623_1484) 3.2.1.78 pubmed
Micromonospora rifamycinica DSM 44983 SCG47572.1 A0A120F9Z2   40
 β-D-galactofuranosidase (GlfH1;Rv309) 3.2.1.146 pubmed
Mycobacterium tuberculosis H37Rv CAB08388.1
AFN51087.1
CCP45905.1
NP_217612.1
I6YB54   13
 α-L-arabinofuranosidase (B160DRAFT_03993) 3.2.1.55 pubmed
Niabella aurantiaca DSM 17617 WP_018627464.1     13
 β-mannanase (TM1) 3.2.1.78 pubmed
Niallia circulans AAT39478.1 Q6IUP2   8
 β-mannanase (Man1;ManB48) 3.2.1.78 pubmed
Niallia circulans CGMCC 1416 AAX87002.1 Q49HI4   8
 β-mannanase (Man5A) 3.2.1.78 pubmed
Niallia circulans CGMCC1554 AAX87003.1 Q0IJR6   8
 β-mannanase (G) 3.2.1.78 pubmed
Niallia circulans K-1 BAA25878.1 O66185   8
 β-mannanase (MANPN11) (fragment) 3.2.1.78 pubmed
Niallia nealsonii PN-11 AGU71466.2
AGU71466.1
AGU71466.3
T1Z249   8
 β-mannosidase (Op5Man5; OPIT5_10225) 3.2.1.25 pubmed
Opitutaceae bacterium TAV5 AHF90519.1 W0J1H8 7BOB[A,B,C]
 endo-β-1,4-glucanase A (CelA) 3.2.1.4 pubmed
Paenibacillus barcinonensis BP-23 CAA73113.1 O08342   4
 endo-β-1,4-glucanase B (CelB;EG-B) 3.2.1.4 pubmed
Paenibacillus lautus PL236 AAA22408.1
CAA01934.1
P23550   4
 xyloglucanase (XG5; PpXG5) 3.2.1.151 pubmed
Paenibacillus pabuli AAR65335.1 H9KVH3 2JEP[A,B]
2JEQ[A]
4
 endo-β-1,4-glucanase 3.2.1.4 pubmed
Paenibacillus polymyxa AAA22631.1
AAY03292.1
P23548   1
 cellulase 5A (Cel5A) 3.2.1.4 pubmed
Paenibacillus polymyxa GS01 ABV08875.1 A9Q741   2
 cellulase 5B (Cel5B) 3.2.1.4 pubmed
Paenibacillus polymyxa GS01 ABV08876.1 A9Q742   4
 endo-β-1,4-glucanase (Endo5A) 3.2.1.4 pubmed
Paenibacillus sp. ICGEB2008 / MTCC 5639 AEB00655.1 G0YA73   1
 endo-β-1,4-xylanase (Pjdr2_4271) 3.2.1.8 pubmed
Paenibacillus sp. JDR-2 ACT02895.1 C6D792   35
 endo-β-1,4-glucanase (Egl) 3.2.1.4 URL
Paenibacillus sp. KCTC8848P AAL83749.1 Q8RPZ3   1
 xyloglucanase (XEG5) (Xeg5) 3.2.1.151 pubmed
Paenibacillus sp. KM21 BAE44526.1 Q3MUH8   4
 endo-β-1,4-glucanase (Cel5A) 3.2.1.4 pubmed
Paenibacillus xylanilyticus KJ-03 AFC68970.1 H9A9K4   4
 endo-β-1,4-glucanase N (CelN) (fragment) 3.2.1.4 pubmed
Pectobacterium carotovorum ATROSEPTICA AAC37033.1 Q59394   2
 endo-β-1,4-glucanase V1 3.2.1.4 pubmed
Pectobacterium carotovorum SCC3193 CAA55823.1 Q59395   2
 endo-β-1,4-glucanase V 3.2.1.4 pubmed
Pectobacterium carotovorum SCRI193 CAA53592.1 Q47096   2
 endo-β-1,4-glucanase (CelA) 3.2.1.4 pubmed
Pectobacterium carotovorum subsp. carotovorum LY34 AAC02964.2
AAC02964.1
O31029   2
 endo-xyloglucanase (Bacsa_0886) 3.2.1.151 pubmed
Phocaeicola salanitronis DSM 18170 ADY35478.1 F0R2T3 6WQY[A] 4
 cellulase 3.2.1.4 pubmed
Photobacterium panuliri LBS5T ALC76666.1 A0A0M4FQ56  
 xylanase (ORF0336) (Xyn5B) 3.2.1.8 pubmed
Prevotella bryantii B14 ADI70667.1 D7SFG3   21
 xylanase (ORF0150) (Xyn5A) 3.2.1.8 pubmed
Prevotella bryantii B14 ADI70668.1 D7SFG4   21
 mixed-linkage β-glucanase/xyloglucanase (Orf4; CMCase; PbrCel5A; PBR_0368) (Cel5A) 3.2.1.151
3.2.1.73
pubmed
pubmed
Prevotella bryantii B14 AAC97596.1
AAA22909.1
D8DY19
O06842
Q44878
3L55[A,B]
3VDH[A,B]
5D9M[A,B]
5D9N[A,B]
5D9O[A,B]
5D9P[A,B]
6D2W[A,B]
4
 endo-β-1,4-glucanase (PRU_1856) 3.2.1.4 doi
Prevotella ruminicola 23 ADE83057.1
BAA74515.1
D5EU37
Q9ZN63
  38
 xylanase / endoglucanase (PRU_2231) 3.2.1.4
3.2.1.8
pubmed
pubmed
Prevotella ruminicola 23 AAC36862.1
ADE83418.1
D5EV54
Q45397
  4
 endoglucanase (CelA;BrEND) 3.2.1.4 pubmed
Prevotella ruminicola AR20 1814455A     4
 glucanase (BglC) 3.2.1.4 pubmed
Priestia megaterium L103 AGG91154.1 V5JAY7   2
 endoglucanase (CelG) (Cel5G) 3.2.1.4 pubmed
Pseudoalteromonas haloplanktis A23 CAA76775.1 O86099 1TVN[A,B]
1TVP[A,B]
2
 endoglucanase (CelA) 3.2.1.4 pubmed
Pseudoalteromonas sp. MB-1 AAS58467.1 Q6QA02   2
 cellulase (CelY) 3.2.1.4 pubmed
Pseudoalteromonas sp. NO3 ACN62172.1 C0KZC4   2
 α-L-arabinofuranosidase (weak activity) (SAMN05216221_3467) 3.2.1.55 pubmed
Pseudomonas oryzae KCTC 32247 SDT09889.1 A0A1H1XM60   45
 bifunctional β-N-acetylglucosaminidase / chitosan-active enzyme (fragment) 3.2.1.132
3.2.1.52
pubmed
pubmed
Pseudomonas putida P3(4) ADG43125.1 D6R436   48
 endoglucanase (CelX) (Cel5M) 3.2.1.4 pubmed
Pseudomonas sp. MM15 AEM45646.1 G3EWG4   5
 cellulase (AclA) 3.2.1.4 doi
Pseudomonas sp. ND137 BAB79288.1     2
 β-1,4-mannanase (AmnA) 3.2.1.78   Pseudomonas sp. ND137 BAB79290.2     8
 endo-β-1,4-glucanase (Cel5A ; Ps_Cel5A; PST_2494) 3.2.1.4 pubmed
Pseudomonas stutzeri A1501 ABP80146.1 A4VME5 4LX4[A,B,C,D]
6R2J[A,B,C,D]
5
 β-mannosidase (Tth Man5;Theth_0949) 3.2.1.25 pubmed
Pseudothermotoga thermarum DSM 5069 AEH51033.1 F7YX66   19
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