Glycoside Hydrolase Family 5

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); β-glycosidase (EC 3.2.1.-); endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); β-N-acetylhexosaminidase (EC 3.2.1.52); chitosanase (EC 3.2.1.132); β-D-galactofuranosidase (EC 3.2.1.146); β-galactosylceramidase (EC 3.2.1.46); ; β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-); α-L-arabinofuranosidase (EC 3.2.1.55); glucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-); hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (24948); Uniprot accession (634); PDB accession (270); 3D entries (100); cryst (0)
| 1 | 2 | 3 | 4 | 5 | 6 |
Eukaryota
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D Subf
 cellulase (CelA;CsCelA) 3.2.1.4 pubmed
Ciboria shiraiana AIC79156.1 A0A068B5I5   5
 endo-β-1,4-mannanase (ManA;CaMANA) 3.2.1.78 pubmed
Coffea arabica CAC08208.1
AAX01860.1
Q9FT02   7
 endo-β-1-4-mannanase (ManB;CaMANB) 3.2.1.78 pubmed
Coffea arabica CAC08442.1 Q9FT03   7
 β-glucocerebrosidase (EGCrP1;CNAG_00623) 3.2.1.45 pubmed
Cryptococcus neoformans var. grubii H99 BAL46040.1
AFR92751.1
AFR92751.2
H1AE12   12
 steryl β-glucosidase (EGCrP2;CNAG_05607) (vacuolar) 3.2.1.104 pubmed
Cryptococcus neoformans var. grubii H99 AFR99035.1 J9W473 7LPO[A,B,C,D]
7LPP[A,B,C,D]
7LPQ[A,B,C,D]
12
 carboxymethylcellulase (CsCMCase) 3.2.1.4 pubmed
Cryptococcus sp. S-2 ABP02069.1 A4UU22   5
 endo-β-1,4-mannanase (Man;CaMan) 3.2.1.78 pubmed
Cryptopygus antarcticus ABV68808.1 B4XC07 4OOU[A,B]
4OOZ[A,B]
10
 endoglycoceramidase (EGCase) 3.2.1.123 pubmed
Cyanea nozakii BAB16369.1
BAB16370.1
Q9GV15
Q9GV16
  27
 endo-β-mannanase (DcMan1) 3.2.1.78 doi
Daucus carota AAN34823.1 Q8H6B4   7
 endo-1,4-β-mannanase 3.2.1.78 pubmed
Eisenia fetida BBB35836.1 A0A2Z5VGG0 5Y6T[A] 10
 β-1,6-glucanase (GcnA) 3.2.1.75 pubmed
Epichloe festucae FL1 ABK27195.1 A0FJV0   15
 β-1,6-glucanase (GcnA) 3.2.1.75 pubmed
Epichloe festucae var. lolii LP19 ABK27199.1 A0FJV4   15
 xylanase/cellulase (CelA) 3.2.1.4 doi
Epidinium caudatum BAC57895.1 Q86S90  
 endoglucanase (Epi2) 3.2.1.4 doi
Epidinium caudatum BAC57893.1 Q86S92   4
 endo-β-1,4-glucanase (Epi3) (probable fragment) 3.2.1.4 doi
Epidinium caudatum BAA76394.1 Q9XXV3   4
 CMCase (Cmc-06) (Cel5A) 3.2.1.4 pubmed
Epidinium ecaudatum CAH69214.1
CAL91975.1
Q5F324   4
 β-mannanase (Man5A) 3.2.1.78 pubmed
Evansstolkia leycettana JCM12802 AJF11663.1 A0A0B5FP75   7
 β-1,6-galactanase (Gal1;FoGal1) 3.2.1.164 pubmed
Fusarium oxysporum 12S BAF42338.1 A0ZSY6   16
 β-1,4-endoglucanase (GlCel5A;GL28282) (Cel5A) 3.2.1.4 pubmed
Ganoderma lucidum   A0A1A9TAJ1 5D8W[A,B]
5D8Z[A,B]
5
 β-mannanase (GVI1; GH5-1) 3.2.1.78 pubmed
Gastrophysa viridula ADU33333.1     10
 β-1,4-endoglucanase (Eng-3) 3.2.1.4 pubmed
Globodera rostochiensis RO-1 AAN03645.1
AAN03646.1
Q8MM42   2
 β-1,4-endoglucanase (Eng-4) 3.2.1.4 pubmed
Globodera rostochiensis RO-1 AAN03647.1
AAN03648.1
Q8MM50   2
 endoglucanase 1 (Eng1;Eng-1;Gr-eng1;Gr-eng-1) 3.2.1.4 pubmed
Globodera rostochiensis RO-1 AAC63988.1
AAC48325.1
O16028
Q7KQF7
  2
 endoglucanase 2 (Eng2;Eng-2) 3.2.1.4 pubmed
Globodera rostochiensis RO-1 AAC48341.1
AAC63989.1
O44078
O77094
  2
 endo-β-1,4-glucanase (GtCel5A) (Cel5A) 3.2.1.4 pubmed
Gloeophyllum trabeum ATCC 11539 ADH51728.1 D7REW1
P84194
  5
 β-mannanase (GLOTRDRAFT_110405) (Man5A) 3.2.1.78 pubmed
Gloeophyllum trabeum ATCC 11539 / CBS900.73 EPQ57046.1 S7QAW1   7
 endo-β-1,4-mannanase (GmMan1) 3.2.1.78 pubmed
Glycine max ABG88068.1 Q0PKY2   7
 GpGH5 / M427DRAFT_29612 3.2.1.- pubmed
Gonapodya prolifera JEL478 KXS18720.1 A0A139APP3   34
 endo-β-1,4-mannanase (Man1;HdMan) 3.2.1.78 pubmed
Haliotis discus hannai BAE78456.1 Q2MH89   10
 endo-β-1,4-glucanase 1a (Eng1A;Eng-1A;Hα-eng-1a) 3.2.1.4 doi
Heterodera avenae ACO55952.1
AFQ55438.1
AGH32769.1
D5FQ93
M4QVR5
  2
 endoglucanase 1 (Eng1;Eng-1) 3.2.1.4 pubmed
Heterodera glycines AAC15707.1
AAC48327.1
CAB42450.1
CAB59144.1
O18453   2
 endoglucanase 2 (Eng2;Eng-2) 3.2.1.4 pubmed
Heterodera glycines AAC48326.1
AAC15708.1
CAB42449.1
CAB59143.1
O18454
O61595
  2
 endoglucanase 3 (Eng3;Eng-3) 3.2.1.4 pubmed
Heterodera glycines AAC33848.1
AAC33860.1
O77449   2
 endo-β-1,4-mannanase (Man1;HvMAN1) 3.2.1.78 pubmed
Hordeum vulgare subsp. vulgare BAK05001.1
ABC87082.1
F2ECC9
Q2I011
  7
 endo-β-1,4-glucanase 3 (CMC3;Egl2) 3.2.1.4 pubmed
pubmed
Humicola grisea var. thermoidea IFO9854 BAA12676.1 Q12624   5
 endo-β-1,4-glucanase IV (CMC3) 3.2.1.4 pubmed
Humicola insolens ATCC 16454 CAA53631.1 Q12624   5
 β-mannanase (alkali-tolerant) (Man5A) 3.2.1.78 pubmed
Humicola insolens Y1 ADZ99301.1 H6TCE0   7
 endoglycoceramidase (EGCase) 3.2.1.123 pubmed
Hydra magnipapillata BAD20464.1 Q6L6S1   27
 β-mannanase (Man1;HhMAN1) 3.2.1.78 pubmed
Hypothenemus hampei ACU52526.1
ACU52527.1
C8CAP6   8
 endoglucanase (Cen1;En-1) 3.2.1.4 pubmed
Irpex lacteus MC-2 BAD67544.1 Q5W7K4   5
 exo-β-1,3-glucanase (Eg;Exg1) 3.2.1.58 pubmed
Komagataella pastoris X-33 AAY28969.1 Q2TCV2   9
 endoglucanase LbGH5-CBM1 3.2.1.4 pubmed
Laccaria bicolor XP_001879429.1 B0D5M7   5
 endo-β-1,4-mannanase (Man1) 3.2.1.78 doi
Lactuca sativa CAC51690.3 Q93X40   7
 exo-β-1,3-glucanase (Exg1) 3.2.1.58 pubmed
Lentinula edodes H600 BAD97445.1
BAD97446.1
Q53UH0   9
 endo-β-1,4-mannanase (LpsGH5_8) 3.2.1.78 pubmed
Lyrodus pedicellatus VAX65844.1     8
 endo-β-1,4-glucanase 1 (Egl1) 3.2.1.4 pubmed
Macrophomina phaseolina AAB51451.1 Q12638   5
 endo-β-1,4-glucanase 2 (Egl2) 3.2.1.4 pubmed
Macrophomina phaseolina AAB03889.1 Q12637   5
 endo-β-1,4-glucanase 1 (Eng-1) 3.2.1.4 pubmed
Meloidogyne incognita AAD45868.1 Q9UA57   2
 β-1,4-endoglucanase (Eng-2) 3.2.1.4 pubmed
Meloidogyne incognita AAK21883.2 Q9BJZ0   2
 endo-β-1,4-glucanase (Eng-1A;Mi-eng-1) 3.2.1.4 pubmed
Meloidogyne incognita AAK21882.1 Q9BJZ1   2
 β-1,4-endoglucanase (Eng-2) 3.2.1.4 pubmed
Meloidogyne incognita AAK21881.1 Q9BJZ2   2
 endo-β-1,4-mannanase (Man5A) 3.2.1.78 pubmed
Mytilus edulis CAC81056.1 Q8WPJ2 2C0H[A] 10
 endo-β-1,4-glucanase A (CelA) 3.2.1.4 pubmed
Neocallimastix frontalis AAC63094.1 Q01409   4
 endo-β-1,4-glucanase B (CelB) 3.2.1.4 pubmed
Neocallimastix patriciarum AAE59927.1
CAA83238.1
Q12647   4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.8
pubmed
pubmed
pubmed
pubmed
pubmed
pubmed
Neocallimastix patriciarum W5 AAC06321.1
AEX92707.1
O59943   4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.8
pubmed
pubmed
pubmed
pubmed
pubmed
pubmed
Neocallimastix patriciarum W5 AAC06321.1 H2BPT1
O59943
  4
 endo-β-1,4-glucanase D (CelD;W5-CelD;W5C-P) (Cel5D) 3.2.1.4
3.2.1.8
pubmed
pubmed
pubmed
pubmed
pubmed
pubmed
Neocallimastix patriciarum W5 AAC06321.1 H2BPT1
O59943
  4
 β-1,6-glucanase 3.2.1.75 pubmed
Neotyphodium sp. FCB-2002 AAN04103.1 Q8NJN4   15
 endo-β-1,4-glucanase (GH5-1;EG2;NCU00762) 3.2.1.4 pubmed
Neurospora crassa OR74A CAE81955.1
ABZ29259.1
XP_324942.1
Q6MGI3
Q7SDR1
  5
 endo-β-1,4-mannanase (NCU08412) 3.2.1.78 pubmed
Neurospora crassa OR74A EAA34048.2     7
 endo-β-1,4-glucanase (CelA) 3.2.1.4 pubmed
Orpinomyces joyonii 19-2 AAC49731.1 P87211   2
 endo-β-1,4-glucanase (CelB2) (fragment) 3.2.1.4 pubmed
Orpinomyces joyonii SG4 AAB69348.1 O13334   4
 endo-β-1,4-glucanase (CelB29) 3.2.1.4 pubmed
Orpinomyces joyonii SG4 AAB69347.1 O13333   4
 β-mannanase (ManA) 3.2.1.78 pubmed
Orpinomyces sp. PC-2 AAL01213.1 Q96V96   7
 endo-β-1,4-glucanase B (CelB) 3.2.1.4 pubmed
Orpinomyces sp. PC-2 AAD04193.1
AAE59925.1
P78719   4
 endo-β-1,4-glucanase E (CelE) 3.2.1.4 pubmed
Orpinomyces sp. PC-2 AAC05164.1 Q9HFE0   4
 exo-β-1,3-glucosidase (GH5BG;Os10g0370500) 3.2.1.58 pubmed
Oryza sativa Japonica Group AAM08614.1
AAM08821.1
AAP53379.1
BAF26372.1
BAG89316.1
BAT10560.1
Q0IY43
Q8RU06
  14
 endo-β-1,4-glucanase (EGb1;EGb2) (Cel5C) 3.2.1.4 doi
Penicillium brasilianum IBT 20888 ACB06750.1 B8Q961   5
 endoglucanase 5C (PdCel5C) (Cel5C) 3.2.1.4 pubmed
Penicillium decumbens 114-2 AFG25592.1 H9TUB0   4
 endoglucanase II / 5A (Eg2;Egl2) (Cel5A) 3.2.1.4 pubmed
Penicillium decumbens 114-2 / JU-A10 ABY28340.1 A9Z054   5
 endoglucanase 1 (Egl1;Pe-Egl1) 3.2.1.4 pubmed
Penicillium echinulatum (nom. illeg.) 9A01S2 ACR82487.1 C5J4L7   5
 endo-β-1,4-glucanase II (Egl2) 3.2.1.4 pubmed
Penicillium janthinellum Biourge IMET 43733 (C41) CAA61740.1 Q12665   5
 β-primeverosidase (Bgl2) 3.2.1.149 pubmed
Penicillium multicolor TS-5 / IAM7153 BAG70961.1 B5MEI8   23
 endo-β-mannanase (Ma;PoMan5A) (Man5A) 3.2.1.78 pubmed
Penicillium oxalicum GZ-2 AGW24296.1 U3N1Q2   7
 β-mannanase (Man5C6;Man5) 3.2.1.78 doi
Penicillium sp. enrichment culture clone C6 / ACCC32495 AEV41143.1 G9BY18   7
 endo-β-1,4-mannanase (Man5F63) 3.2.1.78 pubmed
Penicillium sp. F63 XC-2012 / CGMCC 1669 AFC38441.1 I3PML9   7
 β-xylosidase (Xyl5;PcXyl5;PcGH5) 3.2.1.37   Phanerochaete chrysosporium AHL69750.2
AIA81045.1
W8QRE4   22
 β-mannanase 5D (Man5D) 3.2.1.78 doi
Phanerochaete chrysosporium RP78 ABG79370.1 Q0PQY8   7
 endo-β-1,4-mannanase (Man5AP13) (Man5A) 3.2.1.78 pubmed
Phialophora sp. CGMCC 3329 / P13 ADF28533.1 D5IF02   7
 endo-β-1,4-glucanase (EgG5) 3.2.1.4 pubmed
Phialophora sp. G5 / CGMCC 3328 ADZ44606.1 H6TJC5   5
 exo-1,3-glucanase (Exg1) 3.2.1.58 pubmed
Pichia angusta ATCC 14754 CAA86948.1 Q12626   9
 endoglucanase A (CelA;EglA) (partial) 3.2.1.4 doi
Piromyces rhizinflatus 2301 AAF00074.2 Q9URH5 3AYR[A]
3AYS[A]
4
 endoglucanase (Egl-1) (Cel5B) 3.2.1.4 pubmed
Piromyces rhizinflatus 2301 AAD43818.1 Q9Y870   4
 endoglucanase 5A (Cel5A) (Cel5A) 3.2.1.4
3.2.1.73
pubmed
pubmed
Piromyces sp. equi CAB92326.1 Q9P867   4
 endo-β-1,4-mannanase (PaMan5A;Pa_6_490) (Man5A) 3.2.1.78 pubmed
Podospora anserina S mat+ CAP71606.1
ADO14134.1
CDP31001.1
B2B3C0 3ZIZ[A] 7
 endo-β-1,4-mannanase (Man6;PtrMan6) 3.2.1.78 pubmed
Populus trichocarpa AFX59322.1
EEF05441.1
K7WJ21   7
 endo-β-1,4-glucanase (PpCel5A) (Cel5A) 3.2.1.4 pubmed
Postia placenta Mad-698-R ADH51729.1 D7REW2   5
 endo-β-1,4-glucanase (PpCel5B) (Cel5B) 3.2.1.4 pubmed
Postia placenta Mad-698-R XP_002475436.1
ADH51730.1
B8PID6
D7REW3
  5
 endoglucanase / cellulase (PhEG) 3.2.1.4 pubmed
Psacothea hilaris BAB86867.1 Q8T890   2
 β-1,3-mannanase / β-1,3-glucomannanase (Man5C) 3.2.1.- pubmed
Purpureocillium lilacinum ATCC 36010 BAD06516.1 Q76G11   31
 broad specificity endo-β-glucanase (Egl5A;TeEgl5A) 3.2.1.4 pubmed
Rasamsonia emersonii CBS 394.64 AHV83755.1
CAJ75961.1
X4YXV3   5
 endo-β-1,4-glucanase (Eg1) 3.2.1.4 pubmed
Rasamsonia emersonii IMI 392299 AAL33639.2
AAL33630.1
AAL33630.2
AAL33639.1
Q8WZD7   5
 β-mannanase (RmMan5B) (Man5B) 3.2.1.78 pubmed
Rhizomucor miehei CAU432 AGV01048.1 A0A075C6T6 4LYP[A,B]
4LYQ[A]
4LYR[A]
4NRR[A,B]
4NRS[A,B]
7
 β-mannanase (RmMan5A) (Man5A) 3.2.1.78 pubmed
Rhizomucor miehei CAU432 / CGMCC 4967 AGC24277.1 L7SVX1 4L3M[A]
4QP0[A]
7
 β-glucosylceramidase (EGCrP1;RO3G_04172) 3.2.1.45 pubmed
Rhizopus delemar RA 99-880 EIE79467.1 I1BTD7   12
 endo-β-1,4-glucanase I (Eg1) 3.2.1.4 pubmed
Robillarda sp. Y-20 BAA90480.1
JX0131
P23044
Q9P981
  5
 exo-β-1,3-glucanase (sporulation) (Spr1;Ssg1) 3.2.1.58 pubmed
Saccharomyces cerevisiae GRF88 AAB24895.1
CAA41952.1
CAA99399.1
P32603   9
 exo-β-1,3-glucanase / β-glucosidase (sporulation) (Ssg1;Spr1;YOR190W) 3.2.1.21
3.2.1.58
pubmed
pubmed
Saccharomyces cerevisiae S288c AAU09785.1
NP_014833.1
P32603   9
 exo-β-1,3-glucanase 1 / flavonoid β-glucosidase (Exg1;Bgl1;Scw6;YLR300W) 3.2.1.21
3.2.1.58
doi
pubmed
Saccharomyces cerevisiae S288c AAA34599.1
AAB67345.1
AAT93088.1
NP_013403.1
P23776 1H4P[A,B] 9
 exo-1,3-glucanase 2 (Exg2;YDR261c) 3.2.1.58 pubmed
Saccharomyces cerevisiae S288c CAA86950.1
CAA92719.1
CAA94100.1
NP_010547.1
P52911   9
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