Glycoside Hydrolase Family 5

Known Activities	chitosanase (EC 3.2.1.132); β-mannosidase (EC 3.2.1.25); cellulase (EC 3.2.1.4); glucan β-1,3-glucosidase (EC 3.2.1.58); licheninase (EC 3.2.1.73); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); endo-β-1,4-xylanase (EC 3.2.1.8); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); β-1,3-mannanase (EC 3.2.1.-); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); mannan transglycosylase (EC 2.4.1.-); endo-β-1,6-galactanase (EC 3.2.1.164); endoglycoceramidase (EC 3.2.1.123)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	formerly known as cellulase family A; Following a recent paper by St John et al. (FEBS Letters, 2010, in press) several GH5 subfamilies have been reassigned to GH30.
External resources	CAZypedia; HOMSTRAD; InterPro; PFAM; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (4150); Uniprot accession (1840); PDB accession (110); 3D entries (34); cryst (2)

Summary

All (3017) Archaea (36) Bacteria (1921) Eukaryota (960) Viruses (2) unclassified (98) Structure (34 - 2 cryst) Characterized (374)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,4-glucanase (EGPh;EglB;TCel3;PH1171)	3.2.1.4	Pyrococcus horikoshii OT3	AAQ31832.1 AAQ31833.1 BAA30271.1 NP_143072.1	O58925	2ZUM[A] 2ZUN[A,B,C] 3AXX 3QHM 3QHN 3QHO
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
endo-β-1,4-glucanase E1 (EGAc;EGI;Acel_0614) (Cel5A)	3.2.1.4	Acidothermus cellulolyticus 11B ATCC 43068	AAA75477.1 ABH84883.1 ABK52387.1 ACW33392.1 CAD42489.1 CAR96693.1	P54583	1C0D[A,B] 1ECE[A,B] 1VRX[A,B]
β-mannanase (Man5A)	3.2.1.78	Bacillus agaradhaerens	AAN27517.1 AAN27518.1 AAQ31837.1 AAQ79152.1 AAQ79153.1 ABC13790.1 ABC13791.1		2WHJ[A] 2WHL[A]
endo-β-1,4-glucanase 5A (Cel5A)	3.2.1.4	Bacillus agaradhaerens	AAC19169.1	O85465.1	1A3H[A] 1E5J[A] 1H11[A] 1H2J[A] 1H5V[A] 1HF5[A] 1HF6[A] 1HF7[A] 1OCQ[A] 1QHZ[A] 1QI0[A] 1QI2[A] 1W3K[A] 1W3L[A] 2A3H[A] 2V38[A] 3A3H[A] 4A3H[A] 5A3H[A] 6A3H[A] 7A3H[A] 8A3H[A]
cellulase (BCE103)	3.2.1.4	Bacillus sp. CBS 670.93	AAV25061.1 CAB58698.1 CAB59165.1 CAD82873.1		1LF1[A]
β-mannanase (Amn5) (Man5A)	3.2.1.78	Bacillus sp. JAMB-602	BAD99527.1	Q4W8M3	1WKY[A]
endo-β-1,4-glucanase K (Egl-K)	3.2.1.4	Bacillus sp. KSM-635	AAA22304.1	P19424.1	1G01[A] 1G0C[A]
β-mannanase (ManA;MAN330) (Man5A)	3.2.1.78	Bacillus sp. N16-5	AAT06599.1	Q5YEX6	3JUG[A]
endo-β-1,4-glucanase (EglS;BglC;Gld;BSU18130;BsCel5;BsCel5A) (Cel5A)	3.2.1.4	Bacillus subtilis subsp. subtilis str. 168	AEJ76923.1 CAA82317.1 CAA97610.1 CAB13696.2 NP_389695.1	P10475.1	3PZT[A,B] 3PZU[A,B] 3PZV[A,B,C,D]
β-mannosidase 5A (Man5A)	3.2.1.25	Cellvibrio mixtus NCIMB 8633	AAS19695.1	Q6QT42	1UUQ[A] 1UZ4[A]
endo-β-1,4-glucanase A (CelA;Cca;CelCcaCcel_1099) (Cel5A)	3.2.1.4	Clostridium cellulolyticum H10 ATCC 35319	AAA23221.1 AAA51444.1 ACL75458.1	P17901.1	1EDG[A]
endo-β-1,4-glucanase / xylanase D (EngD)	3.2.1.4 3.2.1.73 3.2.1.8	Clostridium cellulovorans ATCC 35269	AAA23233.1	P28623.1	3ICG[A,B,C,D] 3NDY[A,B,C,D] 3NDZ[A,B,C,D]
xylanase (M6;CtM6;Cthe_2193) (arabinoxylan-specific) (Xyl5A)	3.2.1.8	Clostridium thermocellum ATCC 27405	ABN53395.1	A3DHG6	2Y8K[A]
endo-β-1,4-glucanase C (LC2;CelC;EgC) (Cel5B)	3.2.1.4	Clostridium thermocellum NCIB 10682EL	AAA23220.1	P07985 P0C2S3	1CEC[A] 1CEN[A] 1CEO[A]
endo-β-1,4-glucanase Z (CelZ;EgZ;Dda3937_02793) (Cel5A=Cel5Z)	3.2.1.4	Dickeya dadantii 3937	ADM99099.1 CAA68604.1	P07103.2	1EGZ[A,B,C]
endoglucanase (Cel5A)	3.2.1.4	Eubacterium cellulosolvens 5	BAE46390.1	Q3LHN3	cryst
endoglucanase (Cel5A)	3.2.1.4	Eubacterium cellulosolvens 5	BAE46390.1	Q3LHN3	cryst
endo-β-1,4-glucanase (FnCel5A;Fnod_1560) (Cel5A)	3.2.1.4	Fervidobacterium nodosum Rt17-B1	ABS61403.1 ADD73709.1	A7HNC0	3NCO[A,B] 3RJX[A]
xyloglucanase (XG5;PpXG5)	3.2.1.151	Paenibacillus pabuli	AAR65335.1		2JEP[A,B] 2JEQ[A]
endo-β-1,4-glucanase (Orf4;CMCase)	3.2.1.4	Prevotella bryantii B14	AAA22909.1 AAC97596.1	O06842 Q44878	3L55[A,B] 3VDH[A,B]
endoglucanase (CelG) (Cel5G)	3.2.1.4	Pseudoalteromonas haloplanktis A23	CAA76775.1	O86099	1TVN[A,B] 1TVP[A,B]
endoglycoceramidase II (EGCase II)	3.2.1.123	Rhodococcus sp. M-777	AAB67050.1	O33853	2OSW[A,B] 2OSX[A] 2OSY[A,B] 2OYK[A,B] 2OYL[A,B] 2OYM[A,B]
β-mannanase (Man)	3.2.1.78	Thermobifida fusca KW3	CAA06924.1	Q9ZF13	1BQC[A] 2MAN[A] 3MAN[A]
endo-β-1,4-glucanase 5A (E5;CelE;Tfu_0901) (Cel5A)	3.2.1.4	Thermobifida fusca YX	AAC09379.1 AAZ54939.1	Q01786 Q47RH8	2CKR[A,B] 2CKS[A,B]
endoglucanase / β-mannanase (TM1751) (Cel5A)	3.2.1.4 3.2.1.78	Thermotoga maritima MSB8	AAD36816.1 NP_229549.1	Q9X273	3AMC[A,B] 3AMD[A,B,C,D] 3AMG[A,B] 3AOF[A,B] 3AZR[A,B] 3AZS[A,B] 3AZT[A,B,C,D] 3MMU[A,B,C,D,E,F,G,H] 3MMW[A,B,C,D]
lichenase / endo-mannanase (TM1752;TM_1752) (Cel5B)	3.2.1.73 3.2.1.78	Thermotoga maritima MSB8	AAD36817.1 NP_229550.1	Q9X274	1VJZ[A]
endo-β-1,4-mannanase (Man;TpMan;Tpet_1542)	3.2.1.78	Thermotoga petrophila RKU-1	ABQ47550.1 YP_001245126.1	A5IMX7	3PZ9[A] 3PZG[A] 3PZI[A] 3PZM[A] 3PZN[A] 3PZO[A] 3PZQ[A]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
exo-β-1,3-glucanase (Xog;Xog1;Exg;Ca49c10.05)	3.2.1.58	Candida albicans	AAV05307.1 CAA21969.1 CAA39908.1	P29717.3	1CZ1[A] 1EQC[A] 1EQP[A] 2PB1[A] 2PBO[A] 2PC8[A] 2PF0[A] 3N9K[A] 3O6A[A]
endo-β-1,4-glucanase II (EG2;EGII;Egl2;EG-II) (formerly EGIII) (Cel5A)	3.2.1.4	Hypocrea jecorina	AAA34213.1 AAQ24883.1 AAR60515.1 ABA64553.1 ABE22094.1	P07982.1	3QR3[A,B]
β-mannanase (Man5A)	3.2.1.78	Hypocrea jecorina RUTC-30	AAA34208.1 ABJ41268.1	Q99036	1QNO[A] 1QNP[A] 1QNQ[A] 1QNR[A] 1QNS[A]
endo-β-1,4-mannanase (Man5A)	3.2.1.78	Mytilus edulis	CAC81056.1	Q8WPJ2	2C0H[A]
endoglucanase A (CelA;EglA)	3.2.1.4	Piromyces rhizinflatus 2301	AAF00074.2		3AYR[A] 3AYS[A]
exo-β-1,3-glucanase 1 / flavonoid β-glucosidase (Exg1;Bgl1;Scw6;YLR300W)	3.2.1.21 3.2.1.58	Saccharomyces cerevisiae S288c	AAA34599.1 AAB67345.1 AAT93088.1 NP_013403.1	P23776.1	1H4P[A,B]
endo-β-mannanase 4a / mannan transglycosylase/hydrolase (Man4;LeMan4;LeMAN4a;LeMAN4i;MTH)	2.4.1.- 3.2.1.78	Solanum lycopersicum	AAK56557.1 AAK97759.2 AAK97760.1	Q8L5J1 Q8RVL3 Q93WT4	1RH9[A]
endo-β-1,4-glucanase (Eg1;Eng1)	3.2.1.4	Thermoascus aurantiacus IFO 9748	AAL16412.1 AAL88714.2 ACE10214.1 CAT02251.1 CAZ67882.1	Q8TG26 Q96UI5	1GZJ[A,B] 1H1N[A,B]