Glycoside Hydrolase Family 5

Activities in Family	endo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); β-glycosidase (EC 3.2.1.-); endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); β-N-acetylhexosaminidase (EC 3.2.1.52); chitosanase (EC 3.2.1.132); β-D-galactofuranosidase (EC 3.2.1.146); β-galactosylceramidase (EC 3.2.1.46); ; β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-); α-L-arabinofuranosidase (EC 3.2.1.55)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (18214); Uniprot accession (1920); PDB accession (233); 3D entries (83); cryst (0)

Summary

All (16755) Archaea (99) Bacteria (13891) Eukaryota (2626) Viruses (13) unclassified (126) Structure (83) Characterized (564)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
β-1,4-glucanase (Cel5I;Ccel_0428) (Cel5I)	3.2.1.4	Ruminiclostridium cellulolyticum H10	ACL74811.1 AAL79562.1	B8I5Z4 Q8RLT7		2
endo-β-1,4-glucanase A (CelA;Cca;CelCcaCcel_1099) (Cel5A)	3.2.1.4	Ruminiclostridium cellulolyticum H10	AAA23221.1 AAA51444.1 ACL75458.1	P17901	1EDG[A]	4
cellulase N (CelN; Ccel_0740) (Cel5N)	3.2.1.4	Ruminiclostridium cellulolyticum H10	ACL75118.1 AAG45162.1	B8I7W1 Q9EYQ0		1
mannanase K (ManK;Ccel_0736) (Man5K)	3.2.1.78	Ruminiclostridium cellulolyticum H10	ACL75115.1 AAG45159.1	B8I7V8 Q9EYQ3		17
endo-β-1,4-glucanase 5A (CelA) (Cel5A)	3.2.1.4	Ruminiclostridium josui	BAA12826.1 BAA12826.2	Q59290		2
mannanase 5A (Man5A)	3.2.1.78	Ruminiclostridium josui	BAI52931.1	D1MXA0		17
endo-1,4-glucanase 4 (EGIV)	3.2.1.4	Ruminococcus albus	BAA32286.1	Q07940		2
Rumal_1082 (Cel5D)	3.2.1.4	Ruminococcus albus 7 = DSM 20455	ADU21608.1	E0L8I8		4
lichenase (Ra0453;CUS_6741) (β-1,3-specific;cellobiose releasing)	3.2.1.73	Ruminococcus albus 8	ZP_08159266.1 EGC02962.1			37
lichenase (Ra2830;CUS_5609)	3.2.1.73	Ruminococcus albus 8	EGC04285.1 ZP_08157835.1			1
endo-1,4-glucanase I (EGI)	3.2.1.4	Ruminococcus albus F-40	AAA26469.1	P16216		4
endo-1,4-glucanase V (EgV)	3.2.1.4	Ruminococcus albus F-40	BAA92146.1	Q9LCC5		4
β-1,4-endoglucanase VII (EgVII) (Cel5D)	3.2.1.4	Ruminococcus albus F-40	BAA92430.1	Q9LCC4		4
endo-β-1,4-glucanase A (CelA;EgA)	3.2.1.4	Ruminococcus albus SY3	AAA26467.1 CAA38692.1	P23660 Q59733		4
endo-β-1,4-glucanase B (CelB;EgB)	3.2.1.4	Ruminococcus albus SY3	CAA38693.1	P23661		4
endo-β-1,4-glucanase (GH5B;RUM_06330) (Cel5B)	3.2.1.4	Ruminococcus champanellensis 18P13 = JCM 17042	CBL16847.1	D4LB52		1
endo-β-1,4-glucanase (GH5A;RUM_02800) (Cel5A)	3.2.1.4	Ruminococcus champanellensis 18P13 = JCM 17042	CBL16523.1	D4LA78		4
β-mannanase (GH5C;RUM_21650) (Man5A)	3.2.1.78	Ruminococcus champanellensis 18P13 = JCM 17042	CBL18180.1	D4LEY5		8
endo-β-1,4-glucanase A (EndA)	3.2.1.4	Ruminococcus flavefaciens 17	CAB05881.1 AAB19708.1	O05143 Q53302		4
cellodextrinase (CelA)	3.2.1.74	Ruminococcus flavefaciens FD-1	CAA36207.1	P16169		37
endo-β-1,4-mannanase (Sde_1121) (Gly5R)	3.2.1.78	Saccharophagus degradans 2-40	ABD80383.1	Q21LP6		41
endo-β-1,4-glucanase 5F (SdGluc5_26A;Sde_1572) (Cel5F)	3.2.1.4	Saccharophagus degradans 2-40	ABD80834.1	Q21KE5	5A8M[A,B,C] 5A8N[A] 5A8O[A] 5A8P[A] 5A8Q[A] 5A94[A,B,C,D,E,F] 5A95[A,B,C]	26
endo-β-1,4-glucanase 5B (Sde_2490) (Cel5B)	3.2.1.4	Saccharophagus degradans 2-40	ABD81750.1	Q21HS9
endo-β-1,4-glucanase 5J (Sde_2494) (processive) (Cel5J)	3.2.1.4	Saccharophagus degradans 2-40	ABD81754.1	Q21HS5		2
endo-β-1,4-glucanase 5D (Sde_2636) (Cel5D)	3.2.1.4	Saccharophagus degradans 2-40	ABD81896.1	Q21HD3		4
endo-β-1,4-glucanase 5E (Sde_2929) (Cel5E)	3.2.1.4	Saccharophagus degradans 2-40	ABD82186.1	Q21GJ3
β-1,3-1,6-endoglucanase (Sde_3023) (Gly5M)	3.2.1.39 3.2.1.75	Saccharophagus degradans 2-40	ABD82280.1	Q21G99		47
endo-β-1,4-glucanase 5H (Sde_3237) (processive) (Cel5H)	3.2.1.4	Saccharophagus degradans 2-40	ABD82494.1	Q21FN5		2
endo-β-1,4-glucanase 5G (Sde_3239) (processive) (Cel5G)	3.2.1.4	Saccharophagus degradans 2-40	ABD82496.1	Q21FN3		2
cellulase (CelMytB)	3.2.1.4	Saccharophagus sp. Myt-1	BAM21527.1			2
cellulase (EngV) (fragment)	3.2.1.4	Salinivibrio sp. NTU-05	ACJ71329.1	B8XGR7		4
β-mannanase (Man5A)	3.2.1.78	Salipaludibacillus agaradhaerens	AAN27517.1 AAN27518.1 AAQ31837.1 AAQ79152.1 AAQ79153.1 ABC13790.1 ABC13791.1		2WHJ[A] 2WHL[A]	8
endo-β-1,4-glucanase 5A (Cel5A)	3.2.1.4	Salipaludibacillus agaradhaerens	AAC19169.1	O85465	1A3H[A] 1E5J[A] 1H11[A] 1H2J[A] 1H5V[A] 1HF5[A] 1HF6[A] 1HF7[A] 1OCQ[A] 1QHZ[A] 1QI0[A] 1QI2[A] 1W3K[A] 1W3L[A] 2A3H[A] 2V38[A] 3A3H[A] 4A3H[A] 5A3H[A] 6A3H[A] 7A3H[A] 8A3H[A]	2
cellulase (Cel;BagCel)	3.2.1.4	Salipaludibacillus agaradhaerens DSM 8721	CAD61244.1	Q6A3Q5		4
endoglucanase S (EndS)	3.2.1.4	Sinorhizobium meliloti M5N1CS	AAG44364.1	Q9F0G8		5
STHERM_c14520	3.2.1.4	Spirochaeta thermophila DSM 6192	ADN02392.1	E0RT96		2
STHERM_c20620 (StX;StCBM64A)	3.2.1.4	Spirochaeta thermophila DSM 6192	ADN02996.1	E0RQU0		1
β-glucopyranosidase (SBI_01663)	3.2.1.21	Streptomyces bingchenggensis BCW-1	ADI04784.1	D7CFP8		43
chitosanase II (ChoII)	3.2.1.132	Streptomyces griseus HUT 6037	BAC65342.1	Q83VL5		2
β-mannanase (ManA;SlMan)	3.2.1.78	Streptomyces lividans 1326	AAA26710.2	P51529		8
endo-β-1,4-glucanase A (CelA)	3.2.1.4	Streptomyces lividans 1326		P27035		2
β-1,4-mannanase (Man5S27)	3.2.1.78	Streptomyces sp. S27	ADK91085.1	E2F4D4		8
β-mannanase (SACTE_2347)	3.2.1.78	Streptomyces sp. SirexAA-E	AEN10237.1		4FK9[A]	8
β-mannanase (Man;StMan)	3.2.1.78	Streptomyces thermolilacinus NBRC14274	BAK26781.1		3WSU[A,B] 4Y7E[A,B]	8
β-mannanase (ManII;StManII)	3.2.1.78	Streptomyces thermoluteus NBRC 14269	BAM62868.1			8
bifunctional β-1,4-endoglucanase / cellobiohydrolase (CelAB)	3.2.1.4 3.2.1.91	Teredinibacter turnerae T7901	ABS72374.1	A9Q634		2
endo-β-1,4-glucanase / celulase (CelDZ1a)	3.2.1.4	Thermoanaerobacterium sp. A57Txylan	ALX38276.1		5FIP[A,B,C,D]	2
β-mannanase (Man) (partial)	3.2.1.78	Thermobifida fusca KW3	CAA06924.1	Q9ZF13	2MAN[A] 3MAN[A] 1BQC[A]	8
endoglucanase (EGtf2) (Cel5B)	3.2.1.4	Thermobifida fusca TM51	AAP56348.1	Q7X2N2		1
endomannanase (TM51_04883) (Man5A)	3.2.1.78	Thermobifida fusca TM51	AHB89702.1			8
β-mannanase (Man;TfMan;Tfu-man;Tfu_0900)	3.2.1.78	Thermobifida fusca YX	AAZ54938.1	Q47RH9		8
endoglucanase 5B (Tfu_2712) (Cel5B)	3.2.1.4	Thermobifida fusca YX	AAZ56745.1	Q47LC7		1
endo-β-1,4-glucanase 5A (E5;CelE;TfCel5A;Tfu_0901) (Cel5A)	3.2.1.4	Thermobifida fusca YX	AAZ54939.1 AAC09379.1	Q01786 Q47RH8	2CKR[A,B] 2CKS[A,B]	2
β-mannanase (ManB;TM1227) (Man5)	3.2.1.78	Thermotoga maritima MSB8	AAD36302.1 AAM56792.1 AAM56796.1 ACM94273.1 AGL50158.1 AHD18866.1 AKE29003.1 CAB56854.1 CAH68693.1 CAT81455.1 NP_229032.1	Q9X0V4		7
endoglucanase / β-mannanase (Tm_Cel5A;TM1751) (Cel5A)	3.2.1.4 3.2.1.78	Thermotoga maritima MSB8	AAD36816.1 AGL50683.1 AHD18355.1 AKE29506.1 NP_229549.1	Q9X273	3AMC[A,B] 3AMD[A,B,C,D] 3AMG[A,B] 3AOF[A,B] 3AZR[A,B] 3AZS[A,B] 3AZT[A,B,C,D] 3MMU[A,B,C,D,E,F,G,H] 3MMW[A,B,C,D]	25
lichenase / endo-mannanase (TM1752;TM_1752) (Cel5B)	3.2.1.73 3.2.1.78	Thermotoga maritima MSB8	AAD36817.1 AGL50684.1 AHD18354.1 AKE29507.1 NP_229550.1	Q9X274	1VJZ[A]	36
β-mannanase (ManB) (Man5)	3.2.1.78	Thermotoga neapolitana 5068	CAB56856.1 AAK53459.1	Q9RIK7		7
endo-β-1,4-mannanase (Man;TpMan;TpManGH5;Tpet_1542)	3.2.1.78	Thermotoga petrophila RKU-1	ABQ47550.1 YP_001245126.1	A5IMX7	3PZ9[A] 3PZG[A] 3PZI[A] 3PZM[A] 3PZN[A] 3PZO[A] 3PZQ[A] 6TN6[A]	7
endocellulase (CelA) (Cel5A)	3.2.1.4	Thermus caldophilus GK24	AAK60011.1	Q93Q07		1
exoglucanase	3.2.1.73	uncultured bacterium	AMO13174.1			38
cellulase 5R (Cel5R;Cel5R_FS)	3.2.1.4	uncultured bacterium	AND74761.1		5I2U[A,B]	2
endoglucanase (RBcel1)	3.2.1.4	uncultured bacterium	ACO55737.1	C1JI15	4EE9[A] 4M24[A] 5LJF[A,B]	5
xyloglucan-specific endo-β-1,4-glucanase (XEG5A) (Xeg5A)	3.2.1.151	uncultured bacterium	ACZ54907.1	D2K7Z0	4W84[A,B] 4W85[A,B] 4W86[A,B] 4W87[A,B] 4W88[A,B] 4W89[A,B]	4
bifunctional mannanase-xyloglucanase (MNXEG)	3.2.1.73 3.2.1.78	uncultured bacterium	ADA62505.1	D2KKK4		4
exo-xyloglucanase (XEG5B) (Xeg5B)	3.2.1.-	uncultured bacterium	ADB44000.1	D2XML9	4W8A[A] 4W8B[A]	4
endo-β-1,4-glucanase (Cel5G) (Cel5G)	3.2.1.4	uncultured bacterium	ADD71777.1	D4P8G6
endo-β-1,4-glucanase / acidic cellulase (En1;CMC01D10-ORF18)	3.2.1.4	uncultured bacterium	AEV59734.1			37
EXO02C10-ORF13 (En5)	3.2.1.-	uncultured bacterium	AEV59735.1 AGO64722.1 AGO64723.1 AGO64724.1 AGO64725.1 AGO64726.1 AGO64727.1 AGO64728.1 AGO64729.1 AGO64730.1 AGO64731.1 AGO64732.1			4
endo-β-1,4-glucanase (En3;CMC6A4-ORF12)	3.2.1.4	uncultured bacterium	AEV59725.1			4
Scaffold00024.3 (En4)	3.2.1.-	uncultured bacterium	AEV59731.1			37
cellulase (Exo2b;EXO02C10-ORF14)	3.2.1.4	uncultured bacterium	AEV59736.1			4
Exo_contig136_11 (En6)	3.2.1.-	uncultured bacterium	AEV59739.1			37
cellulase (YBcontig404-00027-37)	3.2.1.4	uncultured bacterium	ADU86901.1			4
cellulase (YBcontig310-00038-19)	3.2.1.4	uncultured bacterium	ADU86902.1			4
β-glucanase (UmCel5G;RC1)	3.2.1.4	uncultured bacterium	ABA42184.1	Q3HXC4		2
β-glucanase (UmCel5H;RC2)	3.2.1.4	uncultured bacterium	ABA42185.1	Q3HXC3		2
endo-β-1,4-glucanase (UmCel5A) (Cel5A)	3.2.1.4	uncultured bacterium	ABB51612.1	Q308B6		5
endo-β-1,4-glucanase (CRS200) (Cel5B)	3.2.1.4	uncultured bacterium	AFJ44728.1			2
endoglucanase / cellulase (cMGL504) (Cel5A)	3.2.1.4	uncultured bacterium	AFJ05146.1		4HTY[A] 4HU0[A]	26
endo-β-1,4-glucanase (CelA) (Cel5A)	3.2.1.4 3.2.1.73	uncultured bacterium	ABA02176.1	Q2I8W1		26
CW-EG1	3.2.1.-	uncultured bacterium	AFS18545.1			44
endoglycosidase (GH6248) (Cel5A)	3.2.1.4 3.2.1.73 3.2.1.78 3.2.1.8	uncultured bacterium	ABB46200.1	Q283N5		4
endo-β-1,4-glucanase (UmCel5F)	3.2.1.4	uncultured bacterium	ABE60666.1	Q1NZ59		39
endo-β-1,4-glucanase (UmCel5L) (Cel5A)	3.2.1.4	uncultured bacterium	ABE60714.1	Q1PDD8		25
cellodextrinase (UmCel5M)	3.2.1.74	uncultured bacterium	ABE60715.1	Q1PDD7		52
endo-β-1,4-glucanase (CelWS3)	3.2.1.4	uncultured bacterium	AFX88673.1			2
endo-β-1,4-glucanase (CelWS14)	3.2.1.4	uncultured bacterium	AFX88668.1			2
endo-β-1,4-glucanase (CelWS6)	3.2.1.4	uncultured bacterium	AFX88666.1			2
endo-β-1,4-glucanase (CelWS30) (fragment)	3.2.1.4	uncultured bacterium	AFX88671.1			2
cellulase (CS10;CelCS10)	3.2.1.4	uncultured bacterium	AGL50932.1			2
endo-β-1,4-glucanase (RC3)	3.2.1.4	uncultured bacterium	ABI94085.1	Q06B38		2
endo-β-1,4-glucanase (RC5)	3.2.1.4	uncultured bacterium	ABI94086.1	Q06B37		2
cellulase (CelE1)	3.2.1.4	uncultured bacterium	AHA42547.2 AHA42547.1		4M1R[A,B]	2
endo-β-1,4-mannosidase (ManEM1)	3.2.1.-	uncultured bacterium	AHC00282.1			7
endoglucanase (CellMM5.1;Cell5.1_3)	3.2.1.4	uncultured bacterium	AID57617.1			25
4348JG28_001	3.2.1.-	uncultured bacterium	ABW39345.1	A9LDT9		52
4348JG48_005	3.2.1.-	uncultured bacterium	ABW39351.1	A9LDU5		52
endoglucanase	3.2.1.4	uncultured bacterium Contig15	AHF23845.1			37
endoglucanase	3.2.1.4	uncultured bacterium Contig33	AHF24998.1			4
endoglucanase (GH5_h)	3.2.1.-	uncultured Bacteroidetes bacterium	AIT97141.1			4
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