Glycoside Hydrolase Family 5

Activities in Familyendo-β-1,4-glucanase / cellulase (EC 3.2.1.4); endo-β-1,4-xylanase (EC 3.2.1.8); β-glucosidase (EC 3.2.1.21); β-mannosidase (EC 3.2.1.25); β-glucosylceramidase (EC 3.2.1.45); glucan β-1,3-glucosidase (EC 3.2.1.58); exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74); glucan endo-1,6-β-glucosidase (EC 3.2.1.75); mannan endo-β-1,4-mannosidase (EC 3.2.1.78); cellulose β-1,4-cellobiosidase (EC 3.2.1.91); steryl β-glucosidase (EC 3.2.1.104); endoglycoceramidase (EC 3.2.1.123); chitosanase (EC 3.2.1.132); β-primeverosidase (EC 3.2.1.149); xyloglucan-specific endo-β-1,4-glucanase (EC 3.2.1.151); endo-β-1,6-galactanase (EC 3.2.1.164); β-1,3-mannanase (EC 3.2.1.-); arabinoxylan-specific endo-β-1,4-xylanase (EC 3.2.1.-); mannan transglycosylase (EC 2.4.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); β-glycosidase (EC 3.2.1.-); endo-β-1,3-glucanase / laminarinase (EC 3.2.1.39); β-N-acetylhexosaminidase (EC 3.2.1.52); chitosanase (EC 3.2.1.132); β-D-galactofuranosidase (EC 3.2.1.146); β-galactosylceramidase (EC 3.2.1.46); ; β-rutinosidase /α-L-rhamnose-(1,6)-β-D-glucosidase (EC 3.2.1.-); α-L-arabinofuranosidase (EC 3.2.1.55); glucomannan-specific endo-β-1,4-glucanase (EC 3.2.1.-)
Mechanism Retaining
ClanGH-A
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseGlu (experimental)
Catalytic Proton DonorGlu (experimental)
NoteOnce known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resourcesCAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; NZYTech; PROZOMIX;
Statistics GenBank accession (21896); Uniprot accession (637); PDB accession (260); 3D entries (99); cryst (0)
All (20436) Archaea (129) Bacteria (16920) Eukaryota (3246) Viruses (15) unclassified (126) Structure (99) Characterized (569)
| 1 | 2 | 3 | 4 | 5 | 6 |
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3D Subf
 mannanase 5A (Man5A) 3.2.1.78 Ruminiclostridium josui BAI52931.1 D1MXA0   17
 endo-1,4-glucanase 4 (EGIV) 3.2.1.4 Ruminococcus albus BAA32286.1 Q07940   2
 lichenase (Ra0453;CUS_6741) (β-1,3-specific;cellobiose releasing) 3.2.1.73 Ruminococcus albus 8 ZP_08159266.1
EGC02962.1
E9SCY7   37
 lichenase (Ra2830;CUS_5609) 3.2.1.73 Ruminococcus albus 8 EGC04285.1
ZP_08157835.1
E9S8W4   1
 endo-1,4-glucanase I (EGI) 3.2.1.4 Ruminococcus albus F-40 AAA26469.1 P16216   4
 endo-1,4-glucanase V (EgV) 3.2.1.4
3.2.1.73
3.2.1.8
Ruminococcus albus F-40 BAA92146.1 Q9LCC5   4
 β-1,4-endoglucanase VII (EgVII) (Cel5D) 3.2.1.4 Ruminococcus albus F-40 BAA92430.1 Q9LCC4   4
 endo-β-1,4-glucanase A (CelA;EgA) 3.2.1.4
3.2.1.8
Ruminococcus albus SY3 AAA26467.1
CAA38692.1
P23660
Q59733
  4
 endo-β-1,4-glucanase B (CelB;EgB) 3.2.1.4 Ruminococcus albus SY3 CAA38693.1 P23661   4
 glucomannanase (RUM_05520) 3.2.1.- Ruminococcus champanellensis 18P13 = JCM 17042 CBL16772.1 D4LAX7 B[nd]
C[nd]
D[nd]
6WQP[A]
6WQV[A]
4
 endo-β-1,4-glucanase (GH5B;RUM_06330) (Cel5B) 3.2.1.4 Ruminococcus champanellensis 18P13 = JCM 17042 CBL16847.1 D4LB52   1
 endo-β-1,4-glucanase (GH5A;RUM_02800) (Cel5A) 3.2.1.4 Ruminococcus champanellensis 18P13 = JCM 17042 CBL16523.1 D4LA78   4
 β-mannanase (GH5C;RUM_21650) (Man5A) 3.2.1.78 Ruminococcus champanellensis 18P13 = JCM 17042 CBL18180.1 D4LEY5   8
 glucomannanase (RfGH5-1/2) 3.2.1.- Ruminococcus flavefaciens WP_009984467.1   B[nd]
6XSU[A]
4
 glucomannanase (RfGH5-1/2) 3.2.1.- Ruminococcus flavefaciens WP_009984467.1     8
 endo-β-1,4-glucanase A (EndA) 3.2.1.4 Ruminococcus flavefaciens 17 CAB05881.1
AAB19708.1
O05143
Q53302
  4
 cellodextrinase (CelA) 3.2.1.74 Ruminococcus flavefaciens FD-1 CAA36207.1 P16169   37
 endo-β-1,4-mannanase (Sde_1121) (Gly5R) 3.2.1.78 Saccharophagus degradans 2-40 ABD80383.1 Q21LP6   41
 endo-β-1,4-glucanase 5F (SdGluc5_26A;Sde_1572) (Cel5F) 3.2.1.4 Saccharophagus degradans 2-40 ABD80834.1 Q21KE5 5A8M[A,B,C]
5A8N[A]
5A8O[A]
5A8P[A]
5A8Q[A]
5A94[A,B,C,D,E,F]
5A95[A,B,C]
26
 endo-β-1,4-glucanase 5B (Sde_2490) (Cel5B) 3.2.1.4 Saccharophagus degradans 2-40 ABD81750.1 Q21HS9  
 endo-β-1,4-glucanase 5J (Sde_2494) (processive) (Cel5J) 3.2.1.4 Saccharophagus degradans 2-40 ABD81754.1 Q21HS5   2
 endo-β-1,4-glucanase 5D (Sde_2636) (Cel5D) 3.2.1.4 Saccharophagus degradans 2-40 ABD81896.1 Q21HD3   4
 endo-β-1,4-glucanase 5E (Sde_2929) (Cel5E) 3.2.1.4 Saccharophagus degradans 2-40 ABD82186.1 Q21GJ3  
 endoglucanase 5A (Sde_3003) (Cel5A) 3.2.1.4 Saccharophagus degradans 2-40 ABD82260.1 Q21GB9   2
 endoglucanase 5A (Sde_3003) (Cel5A) 3.2.1.4 Saccharophagus degradans 2-40 ABD82260.1 Q21GB9   2
 β-1,3-1,6-endoglucanase (Sde_3023) (Gly5M) 3.2.1.39
3.2.1.75
Saccharophagus degradans 2-40 ABD82280.1 Q21G99   47
 endo-β-1,4-glucanase 5H (Sde_3237) (processive) (Cel5H) 3.2.1.4 Saccharophagus degradans 2-40 ABD82494.1 Q21FN5   2
 endo-β-1,4-glucanase 5G (Sde_3239) (processive) (Cel5G) 3.2.1.4 Saccharophagus degradans 2-40 ABD82496.1 Q21FN3   2
 cellulase (CelMytB) 3.2.1.4 Saccharophagus sp. Myt-1 BAM21527.1 I4DUD7   2
 cellulase (EngV) (fragment) 3.2.1.4 Salinivibrio sp. NTU-05 ACJ71329.1 B8XGR7   4
 β-mannanase (Man5A) 3.2.1.78 Salipaludibacillus agaradhaerens AAN27517.1
AAN27518.1
AAQ31837.1
AAQ79152.1
AAQ79153.1
ABC13790.1
ABC13791.1
G1K3N4
Q5YEX6
2WHJ[A]
2WHL[A]
8
 endo-β-1,4-glucanase 5A (Cel5A) 3.2.1.4 Salipaludibacillus agaradhaerens AAC19169.1 O85465 1A3H[A]
1E5J[A]
1H11[A]
1H2J[A]
1H5V[A]
1HF5[A]
1HF6[A]
1HF7[A]
1OCQ[A]
1QHZ[A]
1QI0[A]
1QI2[A]
1W3K[A]
1W3L[A]
2A3H[A]
2V38[A]
3A3H[A]
4A3H[A]
5A3H[A]
6A3H[A]
7A3H[A]
8A3H[A]
2
 endoglucanase S (EndS) 3.2.1.4 Sinorhizobium meliloti M5N1CS AAG44364.1 Q9F0G8   5
 β-glucopyranosidase (SBI_01663) 3.2.1.21 Streptomyces bingchenggensis BCW-1 ADI04784.1 D7CFP8   43
 β-1,4-mannosidase (SCATT_p07120) 3.2.1.25 Streptomyces cattleya NRRL 8057 = DSM 46488 AEW98905.1 F8JJ04 B[nd]
7LR7[A]
7LR8[A]
18
 chitosanase II (ChoII) 3.2.1.132 Streptomyces griseus HUT 6037 BAC65342.1 Q83VL5   2
 β-mannanase (ManA;SlMan) 3.2.1.78 Streptomyces lividans 1326 AAA26710.2 P51529   8
 endo-β-1,4-glucanase A (CelA) 3.2.1.4 Streptomyces lividans 1326   P27035   2
 β-1,4-mannanase (Man5S27) 3.2.1.78 Streptomyces sp. S27 ADK91085.1 E2F4D4   8
 β-mannanase (SACTE_2347) 3.2.1.78 Streptomyces sp. SirexAA-E AEN10237.1 G2NHM6 4FK9[A] 8
 β-mannanase (Man;StMan) 3.2.1.78 Streptomyces thermolilacinus NBRC14274 BAK26781.1 F5HR99 3WSU[A,B]
4Y7E[A,B]
8
 β-mannanase (ManII;StManII) 3.2.1.78 Streptomyces thermoluteus NBRC 14269 BAM62868.1 K4PWK0   8
 bifunctional β-1,4-endoglucanase / cellobiohydrolase (CelAB) 3.2.1.4
3.2.1.91
Teredinibacter turnerae T7901 ABS72374.1 A9Q634   2
 endo-β-1,4-glucanase / celulase (CelDZ1a) 3.2.1.4 Thermoanaerobacterium sp. A57Txylan ALX38276.1 A0A0U4EBH5
I3VS73
5FIP[A,B,C,D] 2
 β-D-mannanase 3.2.1.78 Thermobifida fusca BCRC1914 AID15578.1 A0A068CD09   8
 β-mannanase (Man) (partial) 3.2.1.78 Thermobifida fusca KW3 CAA06924.1 Q9ZF13 2MAN[A]
3MAN[A]
1BQC[A]
8
 endoglucanase (EGtf2; Cel5B)) (Cel5B) 3.2.1.4 Thermobifida fusca TM51 AAP56348.1 Q7X2N2   1
 β-mannanase (Man;TfMan;Tfu-man;Tfu_0900) 3.2.1.78 Thermobifida fusca YX AAZ54938.1 Q47RH9   8
 endoglucanase 5B (Tfu_2712; Cel5B) (Cel5B) 3.2.1.4 Thermobifida fusca YX AAZ56745.1 Q47LC7   1
 endo-β-1,4-glucanase 5A (E5;CelE;TfCel5A;Tfu_0901) (Cel5A) 3.2.1.4 Thermobifida fusca YX AAZ54939.1
AAC09379.1
Q01786
Q47RH8
2CKR[A,B]
2CKS[A,B]
2
 multidomain cellulase 3.2.1.4 thermophilic anaerobe NA10 BAA22939.1 O24820   1
 β-mannanase (ManB;TM1227) (Man5) 3.2.1.78 Thermotoga maritima MSB8 AAD36302.1
AAM56792.1
AAM56796.1
ACM94273.1
AGL50158.1
AHD18866.1
AKE29003.1
CAB56854.1
CAH68693.1
CAT81455.1
NP_229032.1
Q9RIK9
Q9X0V4
R4P2R8
  7
 endoglucanase / β-mannanase (Tm_Cel5A;TM1751) (Cel5A) 3.2.1.4
3.2.1.78
Thermotoga maritima MSB8 AAD36816.1
AGL50683.1
AHD18355.1
AKE29506.1
NP_229549.1
Q9X273
R4P461
3AMC[A,B]
3AMD[A,B,C,D]
3AMG[A,B]
3AOF[A,B]
3AZR[A,B]
3AZS[A,B]
3AZT[A,B,C,D]
3MMU[A,B,C,D,E,F,G,H]
3MMW[A,B,C,D]
25
 lichenase / endo-mannanase (TM1752;TM_1752) (Cel5B) 3.2.1.78 Thermotoga maritima MSB8 AAD36817.1
AGL50684.1
AHD18354.1
AKE29507.1
NP_229550.1
Q9X274
R4P1V9
1VJZ[A] 36
 β-mannanase (ManB) (Man5) 3.2.1.78 Thermotoga neapolitana 5068 CAB56856.1
AAK53459.1
Q9RIK7   7
 endo-β-1,4-mannanase (Man;TpMan;TpManGH5;Tpet_1542) 3.2.1.78 Thermotoga petrophila RKU-1 ABQ47550.1
YP_001245126.1
A5IMX7 3PZ9[A]
3PZG[A]
3PZI[A]
3PZM[A]
3PZN[A]
3PZO[A]
3PZQ[A]
6TN6[A]
7
 endocellulase (CelA) (Cel5A) 3.2.1.4 Thermus caldophilus GK24 AAK60011.1 Q93Q07   1
 exoglucanase 3.2.1.73 uncultured bacterium AMO13174.1 A0A140HJ10   38
 cellulase 5R (Cel5R;Cel5R_FS) 3.2.1.4 uncultured bacterium AND74761.1 A0A172PZJ4 5I2U[A,B] 2
 endoglucanase (RBcel1) 3.2.1.4 uncultured bacterium ACO55737.1 C1JI15 B[nd]
C[nd]
D[nd]
4EE9[A]
4M24[A]
5LJF[A,B]
6ZZ3[A]
5
 xyloglucan-specific endo-β-1,4-glucanase (XEG5A) (Xeg5A) 3.2.1.151 uncultured bacterium ACZ54907.1 D2K7Z0 4W84[A,B]
4W85[A,B]
4W86[A,B]
4W87[A,B]
4W88[A,B]
4W89[A,B]
4
 bifunctional mannanase-xyloglucanase (MNXEG) 3.2.1.73
3.2.1.78
uncultured bacterium ADA62505.1 D2KKK4   4
 exo-xyloglucanase (XEG5B) (Xeg5B) 3.2.1.- uncultured bacterium ADB44000.1 D2XML9 4W8A[A]
4W8B[A]
4
 endo-β-1,4-glucanase (Cel5G) (Cel5G) 3.2.1.4 uncultured bacterium ADD71777.1 D4P8G6  
 cellulase (CL1;C0001) 3.2.1.4 uncultured bacterium ADD82896.1 D4PAK6   4
 β-1,3-glucanase (mrbExg5) 3.2.1.58 uncultured bacterium QPA36577.1   6ZB8[A,B]
6ZB9[A,B]
44
 cellulase (CelA3;BP-CelA3) (fragment) 3.2.1.4 uncultured bacterium AEW10554.1 H2BNN7   2
 endo-β-1,4-glucanase / acidic cellulase (En1;CMC01D10-ORF18) 3.2.1.4 uncultured bacterium AEV59734.1 G9BYB5   37
 EXO02C10-ORF13 (En5) 3.2.1.- uncultured bacterium AEV59735.1
AGO64722.1
AGO64723.1
AGO64724.1
AGO64725.1
AGO64726.1
AGO64727.1
AGO64728.1
AGO64729.1
AGO64730.1
AGO64731.1
AGO64732.1
G9BYB6
S4VGU8
S4VGV3
S4VJK3
S4VJK8
S4VMG2
S4VMG6
  4
 endo-β-1,4-glucanase (En3;CMC6A4-ORF12) 3.2.1.4 uncultured bacterium AEV59725.1 G9BYA6   4
 Scaffold00024.3 (En4) 3.2.1.- uncultured bacterium AEV59731.1 G9BYB2   37
 cellulase (Exo2b;EXO02C10-ORF14) 3.2.1.4 uncultured bacterium AEV59736.1 G9BYB7   4
 Exo_contig136_11 (En6) 3.2.1.- uncultured bacterium AEV59739.1 G9BYC0   37
 cellulase (YBcontig404-00027-37) 3.2.1.4 uncultured bacterium ADU86901.1 H2BCH0   4
 cellulase (YBcontig310-00038-19) 3.2.1.4 uncultured bacterium ADU86902.1 H2BCH1   4
 endo-β-1,4-glucanase (YBcontig310-00424-17) 3.2.1.4 uncultured bacterium ADU86909.1 H2BCH8 6XSO[A,B] 4
 β-glucanase (UmCel5G;RC1) 3.2.1.4 uncultured bacterium ABA42184.1 Q3HXC4   2
 β-glucanase (UmCel5H;RC2) 3.2.1.4 uncultured bacterium ABA42185.1 Q3HXC3   2
 endo-β-1,4-glucanase (UmCel5A) (Cel5A) 3.2.1.4 uncultured bacterium ABB51612.1 Q308B6   5
 endo-β-1,4-glucanase (CRS200) (Cel5B) 3.2.1.4 uncultured bacterium AFJ44728.1 I2B2S2   2
 endoglucanase / cellulase (cMGL504) (Cel5A) 3.2.1.4 uncultured bacterium AFJ05146.1 I6PLH5 4HTY[A]
4HU0[A]
26
 cellulase-xylanase A (Cel5A) 3.2.1.- uncultured bacterium AFO64636.1 I7D7K5   4
 cellulase-xylanase B (Cel5B) 3.2.1.- uncultured bacterium AFO64637.1 I7CY19   4
 endo-β-1,4-glucanase (CelA) (Cel5A) 3.2.1.4
3.2.1.73
uncultured bacterium ABA02176.1 Q2I8W1   26
 endoglycosidase (GH6248) (Cel5A) 3.2.1.4
3.2.1.73
3.2.1.78
3.2.1.8
uncultured bacterium ABB46200.1 Q283N5   4
 endo-β-1,4-glucanase (UmCel5L) (Cel5A) 3.2.1.4 uncultured bacterium ABE60714.1 Q1PDD8   25
 cellodextrinase (UmCel5M) 3.2.1.74 uncultured bacterium ABE60715.1 Q1PDD7   52
 endo-β-1,4-glucanase (CelWS3) 3.2.1.4 uncultured bacterium AFX88673.1 M4I2I3   2
 endo-β-1,4-glucanase (CelWS14) 3.2.1.4 uncultured bacterium AFX88668.1 M4I2I2   2
 endo-β-1,4-glucanase (CelWS6) 3.2.1.4 uncultured bacterium AFX88666.1 M4I2R3   2
 endo-β-1,4-glucanase (CelWS36) 3.2.1.4 uncultured bacterium AFX88672.1 M4I2J6   2
 endo-β-1,4-glucanase (CelWS7) 3.2.1.4 uncultured bacterium AFX88667.1 M4I2J5   2
 cellulase (CS10;CelCS10) 3.2.1.4 uncultured bacterium AGL50932.1 R4P2Q6   2
 endo-β-1,4-glucanase (RC3) 3.2.1.4 uncultured bacterium ABI94085.1 Q06B38   2
 endo-β-1,4-glucanase (RC5) 3.2.1.4 uncultured bacterium ABI94086.1 Q06B37   2
 cellulase (CelE1) 3.2.1.4 uncultured bacterium AHA42547.2
AHA42547.1
A0A0R5P8X1
V5KYY9
4M1R[A,B] 2
 endo-β-1,4-mannosidase (ManEM1) 3.2.1.- uncultured bacterium AHC00282.1 V5W3I5   7
 endoglucanase (CellMM5.1;Cell5.1_3) 3.2.1.4 uncultured bacterium AID57617.1 A0A068F0I2   25
 4348JG28_001 3.2.1.- uncultured bacterium ABW39345.1 A9LDT9   52
 4348JG48_005 3.2.1.- uncultured bacterium ABW39351.1 A9LDU5   52
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