Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-); β-1,2-glucosidase (EC 3.2.1.-); β-1,3-glucosidase (EC 3.2.1.-); xyloglucan-specific exo-β-1,4-glucanase / exo-xyloglucanase (EC 3.2.1.155); stevioside-β-1,2-glucosidase (EC 3.2.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); protodioscin 26-O-β-D-glucosidase (EC 3.2.1.186)
Mechanism Retaining
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that some enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (44223); Uniprot accession (325); PDB accession (175); 3D entries (52); cryst (4)
| 1 | 2 | 3 | 4 |
Archaea
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 β-xylosidase / α-L-arabinosidase (XarS;SSO3032) 3.2.1.37
3.2.1.55
pubmed
pubmed
Saccharolobus solfataricus P2 AAK43134.1
NP_344344.1
Q97UI4  
 β-glucosidase (Bgl;TpBgl;Tpen_1494) 3.2.1.21 pubmed
Thermofilum pendens Hrk 5 ABL78891.1 A1S0B1  
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 β-glucosidase B (BglB;Cthe_1256) 3.2.1.21 doi
Acetivibrio thermocellus ATCC 27405 ABN52488.1 P14002  
 β-glucosidase B (BglB) 3.2.1.21 pubmed
Acetivibrio thermocellus DSM 1237 CAA33665.1 P14002  
 β-glucosidase (BglAm;Amir_2978) 3.2.1.21 pubmed
Actinosynnema mirum DSM 43827 ACU36898.1 C6WRQ6  
 β-glucosidase (EryBI) 3.2.1.21 pubmed
Aeromicrobium erythreum AAU93797.1 Q5Y9H4  
 coniferin β-glucosidase (Cbg1;Cbg-1) 3.2.1.21
3.2.1.126
pubmed
pubmed
Agrobacterium tumefaciens AAA22082.1 P27034  
 β-N-acetylhexosaminidase (Amuc_2109) 3.2.1.52   Akkermansia muciniphila ATCC BAA-835 ACD05918.1
QTD80614.1
  B[nd]
7VI6[A]
7VI7[A]
 β-xylosidase (BglX;TC41_2927) 3.2.1.37 URL
Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 AEJ44817.1 F8IKQ1  
 β-glucosidase B 3.2.1.21 pubmed
Bacillus sp. GL1 BAA36161.1 Q9ZNN6  
 β-N-acetylglucosaminidase (NagZ;YbbD;YzbA;BSU01660) 3.2.1.52 doi
Bacillus subtilis subsp. subtilis str. 168 AAA64351.1
AIY91451.1
AQR80107.1
AQR84320.1
BAA19499.1
CAB11942.1
NP_388047.1
A0A6M4JEW2
P40406
3BMX[A,B]
3CQM[A,B]
3LK6[A,B,C,D]
3NVD[A,B]
4GYJ[A,B]
4GYK[A,B]
 β-glucosidase (P43-9H:P46-4G) 3.2.1.21 pubmed
bacterium enrichment culture clone P43-9H:P46-4G ADY39450.1 F1CYL1  
 β-glucosidase (P69-9E) 3.2.1.21 pubmed
bacterium enrichment culture clone P69-9E ADY39467.1 F1CYM8  
 β-xylosidase (BACINT_01042) 3.2.1.37 pubmed
Bacteroides intestinalis DSM 17393 ZP_03013483.1    
 β-xylosidase (BACINT_00926) (Xyl3A) 3.2.1.37 pubmed
Bacteroides intestinalis DSM 17393 EDV05842.1 B3C8W3  
 β-glucosidase (BACOVA_02738; Bovatus_03146; Bglx_6) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ47754.1
EDO11529.1
A7LY22  
 β-glucosidase (Bovatus_01321; Bglx_1;BACOVA_00625 ZP_02063674.1) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ45967.1    
 Bovatus_03424 (Bglb_4; revised model of BACOVA_00946 ZP_02063987.1) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ48031.1    
 β-glucosidase (BACOVA_02745; Bovatus_03153) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ47761.1
EDO11536.1
A7LY29  
 β-glucosidase (Bglb_3;BoGH3A;BACOVA_02644;Bovatus_03064) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ47672.1    
 β-glucosidase (Bglx_5;BoGH3B;BACOVA_02659;Bovatus_03075) 3.2.1.21 pubmed
Bacteroides ovatus ATCC 8483 ALJ47683.1 A7LXU3 5JP0[A,B]
 β-1,2-glucosidase (BT_3567) 3.2.1.- pubmed
Bacteroides thetaiotaomicron HB-13 AOP17662.1 A0A1C9J7J8  
 β-1,2-glucosidase (BT3567) 3.2.1.- pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO78673.1
NP_812479.1
Q8A1U1 5XXL[A,B]
5XXM[A,B]
5XXN[A,B]
5XXO[A,B]
 β-glucosidase (BT3314;BT_3314) 3.2.1.21 pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO78420.1
NP_812226.1
Q8A2J1  
 exo-β-1,3-glucanase (BuGH3;BACUNI_01484) 3.2.1.- pubmed
Bacteroides uniformis ATCC 8492 ZP_02070067.1    
 β-glucosidase (Bgl8;BuBGL8) (partial) 3.2.1.21 pubmed
Bacteroides uniformis ZL1 AGU13704.1 T1WEH3  
 β-glucosidase (BAD_1194) 3.2.1.21 pubmed
Bifidobacterium adolescentis ATCC 15703 BAF39975.1 A1A2P2 5WAB[A,B,C,D]
 α-L-arabinopyranosidase / β-galactosidase (Apy;Apy-H1) 3.2.1.23
3.2.1.-
pubmed
pubmed
Bifidobacterium longum H-1 ADT80794.1 E7CY69  
 β-glucosidase (BglX) 3.2.1.21 pubmed
Bifidobacterium longum H-1 ADY62498.1 F1DPQ8  
 β-glucosidase / β-xylosidase / multi-functional glycoside hydrolase (BGL;XYL;Blon_0625) 3.2.1.21
3.2.1.37
3.2.1.55
pubmed
pubmed
pubmed
Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088 ACJ51732.1 B7GPK2  
 β-glucosidase (BLNIAS_00597; Bglx2) 3.2.1.21 pubmed
Bifidobacterium longum subsp. longum KACC 91563 AEI96973.1 F8ATF7 5Z9S[A,B]
 BCN122_I0864 (NagZ) 3.2.1.52 pubmed
Burkholderia cenocepacia GIMC4560:Bcn122 ARF84251.1 A0A125HFC0 6DTE[A,B]
 β-glucosidase A (BglA) 3.2.1.21 pubmed
Butyrivibrio fibrisolvens H17C AAA23008.1 P16084  
 β-xylosidase (ORF0541) (Xyl3A) 3.2.1.37 pubmed
Caldanaerobius polysaccharolyticus KMCJ AFM44649.1 I6R6X6  
 β-xylosidase (Cb2487;Cb1172;CbXyl3A;Athe_2354) (Xyl3A) 3.2.1.37 pubmed
Caldicellulosiruptor bescii DSM 6725 ACM61424.1 B9MN93  
 β-glucosidase (Cba) 3.2.1.21 pubmed
Cellulomonas biazotea AAC38196.1 O51843  
 bifunctional N-acetyl-β-glucosaminidase/β-glucosidase (Nag3) (acts as a phosphorylase in presence of phosphate) (Nag3A) 3.2.1.21
3.2.1.52
2.4.1.-
pubmed
pubmed
pubmed
Cellulomonas fimi ATCC 484 AAQ05801.1 Q7WUL3  
 β-xylosidase (CfXyl3A;Celf_2726) (Xyl3A) 3.2.1.37 pubmed
Cellulomonas fimi ATCC 484 AEE46850.1 F4H6T9  
 β-glucosidase (CfBgl3A;Celf_0140) (Bgl3A) 3.2.1.21 pubmed
Cellulomonas fimi ATCC 484 AEE44289.1 F4H4T8  
 β-glycosidase (CfBgl3C;Celf_3372) (Bgl3C) 3.2.1.- pubmed
Cellulomonas fimi ATCC 484 AEE47485.1 F4H1R5  
 β-glucosidase (CfBgl3B;Celf_0468) (Bgl3B) 3.2.1.21 pubmed
Cellulomonas fimi ATCC 484 AEE44608.1 F4H851  
 β-glucosidase (BglA) (fragment) 3.2.1.21 pubmed
Cellulomonas flavigena CDBB531 / PN-120 AFP89333.1 J7H3I9  
 β-glucosidase (CgB) 3.2.1.21 pubmed
Cellulomonas gilvus ATCC13127 BAA03152.1 P96316  
 β-glucosidase (Bgl3A;CJA_0204) (Cel3A) 3.2.1.6 pubmed
Cellvibrio japonicus Ueda107 ACE84847.1 B3PGE9  
 exo-xyloglucanase (Blg3D;CJA_1140) (Cel3D) 3.2.1.155 pubmed
Cellvibrio japonicus Ueda107 ACE82897.1 B3PBS6  
 β-glucosidase (Bgl3B;CJA_1497) (Cel3B) 3.2.1.6
3.2.1.-
pubmed
pubmed
Cellvibrio japonicus Ueda107 ACE83024.1 B3PDN7  
 β-glucosidase (Bgl3C;CJA_0223) (Cel3C) 3.2.1.- pubmed
Cellvibrio japonicus Ueda107 ACE85841.1 B3PGG8  
 β-glucosidase (CMM_0947) 3.2.1.21 pubmed
Clavibacter michiganensis subsp. michiganensis NCPPB 382 CAN00986.1 A5CPI7  
 β-N-acetylglucosaminidase (Nag) (Nag3A) 3.2.1.52 pubmed
Clostridium paraputrificum M-21 BAC56177.1 Q84IZ5  
 β-xylosidase (XylD) 3.2.1.37 pubmed
Corynebacterium alkanolyticum Nakao et al. AJY53618.1 A0A0E3JDE2  
 β-glucosidase / β-xylosidase (BgxA) 3.2.1.21
3.2.1.37
pubmed
pubmed
Dickeya chrysanthemi D1 AAA80156.1 Q46684  
 β-xylosidase (Dtur_0852) 3.2.1.37 pubmed
Dictyoglomus turgidum DSM 6724 ACK42133.1 B8E050  
 β-glucosidase / β-glycosidase (Dtur_0219) 3.2.1.21 pubmed
Dictyoglomus turgidum DSM 6724 ACK41548.1
YP_002352162.1
B8E1M3  
 β-glucosidase (Bgl;Fbgl;FmBGL) 3.2.1.21 doi
Elizabethkingia meningoseptica AAB66561.1 O30713  
 β-glucosidase (BglX) 3.2.1.21 pubmed
Escherichia coli str. K-12 substr. MG1655 AAA60495.1
AAB38487.1
AAC75193.1
AIZ90796.1
ALI40123.1
AMC95086.1
APC52371.1
AUG16880.1
AVI56630.1
AYG18842.1
NP_416636.1
P33363  
 β-N-acetylglucosaminidase (NagZ;YcfO) 3.2.1.52 pubmed
Escherichia coli str. K-12 substr. MG1655 AAC74191.1
ACX49759.1
AIZ91775.1
ALI41114.1
AMC98318.1
AUG15890.1
AVI55625.1
AYG19803.1
BAA35922.1
NP_415625.1
D0EX67
P75949
 
 β-glucosidase (FgBgl3A) (Bgl3A) 3.2.1.21 pubmed
Fervidobacterium gondwanense AB39 AFY97405.1 K9URL4  
 Fisuc_2065 / FSU_2592 3.2.1.21 pubmed
doi
Fibrobacter succinogenes subsp. succinogenes S85 ACX75652.1
ADL25584.1
C9RJD5  
 β-glucosidase (BglF3;Fjoh_0775) 3.2.1.21 pubmed
Flavobacterium johnsoniae UW101 ABQ03809.1 A5FLV3  
 β-glucosidase (BglBX10;Fjoh_3392) 3.2.1.21 pubmed
Flavobacterium johnsoniae UW101 ABQ06406.1 A5FEF5  
 β-xylosidase (BN863_22130) 3.2.1.37 doi
Formosa agariphila KMM 3901 CDF79925.1 T2KMH0  
 β-glucosidase (GOTRE_010_00060) (possible fragment) 3.2.1.21 pubmed
Gordonia terrae NBRC 100016 GAB42172.1    
 β-glycosidase (HaGH03;Haur_1431) 3.2.1.- doi
Herpetosiphon aurantiacus DSM 785 ABX04075.1 A9B3B2  
 β-xylosidase (BxyL) 3.2.1.37 pubmed
Klebsiella sp. T4-1 AMK07469.1 A0A140D6H5  
 β-xylosidase (XylB) 3.2.1.37 pubmed
Klebsiella sp. T5-5 AMK07510.1 A0A140D6K8  
 β-glucosidase (BglxA) 3.2.1.21 pubmed
Komagataeibacter hansenii ATCC 23769 BAB85524.1 Q8RTY7  
 exo-β-1,4-glucosidase / β-glucosidase (β-Glu;G3ase) 3.2.1.21
3.2.1.74
pubmed
pubmed
Komagataeibacter sucrofermentans BPR2001 AAQ38005.1
BAA23595.1
BAA31467.1
O24749  
 β-glucosidase (LVIS_1961) 3.2.1.21 pubmed
Levilactobacillus brevis ATCC 367 ABJ65020.1 Q03P52  
 β-1,2-glucosidase / β-1,3-glucosidase (Lin1840) 3.2.1.-
3.2.1.-
pubmed
pubmed
pubmed
pubmed
Listeria innocua Clip11262 CAC97071.1
NP_471175.1
Q92AS9 4ZO6[A,B]
4ZO7[A,B]
4ZO8[A,B]
4ZO9[A,B]
4ZOA[A,B]
4ZOB[A,B]
4ZOC[A,B]
4ZOD[A,B]
4ZOE[A,B]
 β-glucosidase (Gluc3M) 3.2.1.21 pubmed
Martelella mediterranea gluc3m ADC53302.1 D3Y125  
 ginsenoside β-glucosidase (Bgp3) 3.2.1.21 pubmed
Microbacterium esteraromaticum GS514 / KACC16318 AEX88467.1 H2DGJ4  
 ginsenoside β-glucosidase (Bgp1) 3.2.1.21 pubmed
Microbacterium esteraromaticum KACC16318 AEX88466.1 H2DGJ3  
 ginsenoside β-glucosidase (BglQM;Bgl-QM49-1) 3.2.1.21 pubmed
Mucilaginibacter sp. QM49 AFS34656.1 J9Y3X3  
 β-N-acetylglucosaminidase (Rv0237;LpqI) 3.2.1.52 pubmed
Mycobacterium tuberculosis H37Rv CAA17329.1
AFN48090.1
CAE55256.1
CCP42966.1
NP_214751.1
L7N6B0 6GFV[A,B]
 β-glucosidase (SalB) 3.2.1.21 pubmed
Niveispirillum irakense KBC1 AAF21799.1 Q9RH02  
 β-glucosidase (SalA) 3.2.1.21 pubmed
Niveispirillum irakense KBC1 AAF21798.1 Q9RH03  
 cellobiase (CelA) 3.2.1.21 pubmed
Niveispirillum irakense KBC1 AAG43575.1 Q9F0N9  
 β-glucosidase (SBGL) 3.2.1.21 pubmed
Novosphingobium sp. GX9 AGS08637.1 S5R8R0  
 β-glucosidase (OENOO_34001) 3.2.1.21 pubmed
Oenococcus oeni ATCC BAA-1163 EAV39986.1
ZP_01543735.1
A0NHN3  
 isoprimeverose-producing oligoxyloglucan hydrolase (IPase) 3.2.1.120 doi
Oerskovia sp. Y1 BAM08953.1 I0IUK4  
 β-N-acetyl-D-glucosaminidase 3.2.1.52 pubmed
Paenibacillaceae   A0A5S8WF56 6K5J[A]
 β-glucosidase (PstG;PSTG) 3.2.1.21 pubmed
Paenibacillus relictisesami BAJ09393.1 D6RVX0  
 β-glucosidase (BglY) 3.2.1.21 pubmed
Paenibacillus sp. C7 AAX35883.1 Q58PF3  
 glucocerebrosidase 3.2.1.45 pubmed
Paenibacillus sp. TS12 BAC16750.1 Q8GAT9  
 β-glucosidase (BglA) 3.2.1.21 pubmed
Paenibacillus xylanilyticus KJ-03 AFC68969.1 H9A9K3  
 exo-β-1,4-glucosidase (CdxA;Orf0348;PBR_0352;00348-GPBOY) 3.2.1.21
3.2.1.74
pubmed
pubmed
Prevotella bryantii B14 AAA86753.2
AAA86753.1
Q59716  
 β-xylosidase (Orf00398;PBR_0403;00398-GPBOY) (Xyl3A) 3.2.1.37 pubmed
Prevotella bryantii B14 ADD92014.1 D5KJA3  
 β-xylosidase (Orf0911;PBR_0906;00911-GPBOY) (Xyl3B) 3.2.1.37 pubmed
Prevotella bryantii B14 ADD92015.1 D5KJA4  
 β-xylosidase (Orf0975;PBR_0972;00975-GPBOY) (Xyl3C) 3.2.1.37 pubmed
Prevotella bryantii B14 ADD92016.1 D5KJA5  
 β-xylosidase / α-L-arabinofuranosidase (Xyl3A; PRU_2730) (Xyl3A) 3.2.1.37
3.2.1.55
pubmed
pubmed
Prevotella ruminicola 23 ACN78955.1
ADE82109.1
D5EY15  
 β-N-acetylglucosaminidase A (Cht60) 3.2.1.52 pubmed
Pseudoalteromonas piscicida O-7 BAA04223.1
I39596
P48823  
 exo-1,3/1,4-β-glucanase (GluA;ExoP) 3.2.1.- pubmed
Pseudoalteromonas sp. BB1 ABC88234.1 Q0QJA3 3F93[A,B,C,D]
3F94[A]
3RRX[A]
3USZ[A]
3UT0[A,B,C,D]
 β-N-acetylglucosaminidase (NagZ;PA3005) 3.2.1.52 pubmed
Pseudomonas aeruginosa PAO1 AAG06393.1
NP_251695.1
Q9HZK0 5G1M[A,B]
5G2M[A,B]
5G3R[A,B]
5G5K[A,B]
5G5U[A,B]
5G6T[A,B]
5LY7[A,B]
 PA1726 (BglX) 3.2.1.-
3.2.1.-
pubmed
pubmed
pubmed
pubmed
Pseudomonas aeruginosa PAO1 AAG05115.1
NP_250417.1
Q9I311 6R5I[A,B]
6R5N[A,B]
6R5O[A,B]
6R5P[A,B]
6R5R[A,B]
6R5T[A,B]
6R5U[A,B]
6R5V[A,B]
 β-glucosidase (BglPC28;BglG1537b) 3.2.1.21 pubmed
Pseudonocardia sp. Gsoil1536 AGA60134.1 L0EKB3  
 β-xylosidase (XynB3;Theth_0138) 3.2.1.37 pubmed
Pseudothermotoga thermarum DSM 5069 AEH50242.1 F7YUQ7  
 β-N-acetylhexosaminidase (RRR_05655) 3.2.1.52 pubmed
Rickettsia rickettsii str. R AJG33435.1    
 xylan β-1,4-xylosidase (RiXyl3A;ROSINTL182_08202) (Xyl3A) 3.2.1.37 pubmed
Roseburia intestinalis L1-82 EEU99897.1 C7GE50  
 β-glucosidase (RiGH3B; RIL182_00213; ROSINTL182_07684; Bglb_2) 3.2.1.21 pubmed
Roseburia intestinalis L1-82 VCV20358.1
EEV00398.1
C7GCP6  
 β-glucosidase (GH3A; RIL182_00212; ROSINTL182_07685; Bglb_1) 3.2.1.21 pubmed
Roseburia intestinalis L1-82 VCV20357.1
EEV00399.1
C7GCP7  
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