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Glycoside Hydrolase Family 3

Activities in Family[retaining] β-NAcetylglucosaminoside phosphorylase (EC 2.4.1.-);
β-glycan phosphorylase (EC 2.4.1.-);
MLG exo-β-1,4-glucosidase (EC 3.2.1.-);
[retaining] xyloglucan-specific exo-β-1,4-glucosidase (EC 3.2.1.-);
α-L-arabinopyranosidase (EC 3.2.1.-);
Avenacinase (EC 3.2.1.-);
β-1,2-glucosidase (EC 3.2.1.-);
β-1,6-glucosidase (EC 3.2.1.-);
D-4,5-unsaturated β-glucuronyl hydrolase (EC 3.2.1.-);
Exo-β-1,3-1,4-glucanase (EC 3.2.1.-);
Terpenoid-β-1,2-glucosidase (EC 3.2.1.-);
Tomatinase β-1,2-glucosidase (EC 3.2.1.-);
[retaining] isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120);
Coniferin β-glucosidase (EC 3.2.1.126);
Chitosanase (EC 3.2.1.132);
Hesperidin 6-O-α-L-rhamnosyl-β-glucosidase (EC 3.2.1.168);
Protodioscin 26-O-β-D-glucosidase (EC 3.2.1.186);
β-glucosidase (EC 3.2.1.21);
β-galactosidase (EC 3.2.1.23);
β-glucuronidase (EC 3.2.1.31);
Xylan β-1,4-xylosidase (EC 3.2.1.37);
β-D-fucosidase (EC 3.2.1.38);
β-glucosylceramidase (EC 3.2.1.45);
β-N-acetylhexosaminidase (EC 3.2.1.52);
α-L-arabinofuranosidase (EC 3.2.1.55);
Exo-β-1,3-glucanase (EC 3.2.1.58);
Endo-β-1,3(4)-glucanase / lichenase-laminarinase (EC 3.2.1.6);
β-1,3-xylosidase (EC 3.2.1.72);
Lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73);
Exo-β-1,4-glucanase / cellodextrinase (EC 3.2.1.74);
Endo-β-1,4-xylanase (EC 3.2.1.8);
Mechanism Retaining e
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that some enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (64154); Uniprot accession (353); PDB accession (204); 3D entries (59); cryst (3)
Summary
Download GH3 (74557) Taxonomic display Structure (59 - 3 cryst) Characterized (379)
| 1 | 2 | 3 | 4 |
Archaea
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 β-xylosidase / α-L-arabinosidase (XarS;SSO3032) 3.2.1.37
3.2.1.55		 pubmed
pubmed
 Saccharolobus solfataricus P2 AAK43134.1
NP_344344.1		 Q97UI4  
 β-glucosidase (Bgl;TpBgl;Tpen_1494) 3.2.1.21 pubmed
 Thermofilum pendens Hrk 5 ABL78891.1 A1S0B1  
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 β-glucosidase B (BglB;Cthe_1256) 3.2.1.21 doi
 Acetivibrio thermocellus ATCC 27405 ABN52488.1 P14002 7MS2[A,B]
 β-glucosidase B (BglB) 3.2.1.21 pubmed
 Acetivibrio thermocellus DSM 1237 CAA33665.1 P14002  
 β-glucosidase (BglAm;Amir_2978) 3.2.1.21 pubmed
 Actinosynnema mirum DSM 43827 ACU36898.1 C6WRQ6  
 β-glucosidase (EryBI) 3.2.1.21 pubmed
 Aeromicrobium erythreum AAU93797.1 Q5Y9H4  
 coniferin β-glucosidase (Cbg1;Cbg-1) 3.2.1.21
3.2.1.126		 pubmed
pubmed
 Agrobacterium tumefaciens AAA22082.1 P27034  
 β-N-acetylhexosaminidase (Amuc_2109) 3.2.1.52   Akkermansia muciniphila ATCC BAA-835 ACD05918.1
QTD80614.1		 B2UPP0 7VI6[A,B]
7VI7[A,B]
 β-xylosidase (BglX;TC41_2927) 3.2.1.37 URL
 Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1 AEJ44817.1 F8IKQ1  
 N-acetylglucosaminidase (YbbD; BlNagZ; MUY_000173) 3.2.1.52 pubmed
 Bacillus licheniformis WX-02 AKQ71305.1    
 β-N-acetylglucosaminidase (NagZ; BpNagZ) 3.2.1.52 pubmed
 Bacillus pumilus ATCC 7061 QEQ48494.1    
 β-glucosidase B 3.2.1.21 pubmed
 Bacillus sp. GL1 BAA36161.1 Q9ZNN6  
 exo-β-N-acetylglucosaminidase (NagZ; BsNagZ; YbbD; YzbA; BSU01660) 3.2.1.52 doi
 Bacillus subtilis subsp. subtilis str. 168 AAA64351.1
AIY91451.1
AQR80107.1
AQR84320.1
BAA19499.1
CAB11942.1
NP_388047.1		 A0A6M4JEW2
P40406		 3BMX[A,B]
3CQM[A,B]
3LK6[A,B,C,D]
3NVD[A,B]
4GYJ[A,B]
4GYK[A,B]
 β-glucosidase (P43-9H:P46-4G) 3.2.1.21 pubmed
 bacterium enrichment culture clone P43-9H:P46-4G ADY39450.1 F1CYL1  
 β-glucosidase (P69-9E) 3.2.1.21 pubmed
 bacterium enrichment culture clone P69-9E ADY39467.1 F1CYM8  
 β-1,6-glucosidase (Bglx_2; BcellWH2_01926) 3.2.1.21   Bacteroides cellulosilyticus WH2 ALJ59172.1    
 β-xylosidase (BACINT_01042) 3.2.1.37 pubmed
 Bacteroides intestinalis DSM 17393 ZP_03013483.1    
 β-xylosidase (BACINT_00926) (Xyl3A) 3.2.1.37 pubmed
 Bacteroides intestinalis DSM 17393 EDV05842.1 B3C8W3  
 β-glucosidase (BACOVA_02738; Bovatus_03146; Bglx_6) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ47754.1
EDO11529.1		 A7LY22  
 β-glucosidase (Bovatus_01321; Bglx_1;BACOVA_00625 ZP_02063674.1) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ45967.1    
 β-glucosidase (Bovatus_03424; Bglb_4; BACOVA_00946) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ48031.1    
 β-glucosidase (BoGH3MLG; BACOVA_02745; Bovatus_03153) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ47761.1
EDO11536.1		 A7LY29  
 β-glucosidase (Bglb_3;BoGH3A;BACOVA_02644;Bovatus_03064) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ47672.1    
 β-glucosidase (Bglx_5;BoGH3B;BACOVA_02659;Bovatus_03075) 3.2.1.21 pubmed
 Bacteroides ovatus ATCC 8483 ALJ47683.1 A7LXU3 5JP0[A,B]
 β-1,2-glucosidase (BT_3567) 3.2.1.- pubmed
 Bacteroides thetaiotaomicron HB-13 AOP17662.1 A0A1C9J7J8  
 β-glucosidase (BtGlu; BT1780; BT_1780) 3.2.1.21 doi
 Bacteroides thetaiotaomicron VPI-5482 AAO76887.1
NP_810693.1		    
 β-1,2-glucosidase (BT3567) 3.2.1.- pubmed
 Bacteroides thetaiotaomicron VPI-5482 AAO78673.1
NP_812479.1		 Q8A1U1 5XXL[A,B]
5XXM[A,B]
5XXN[A,B]
5XXO[A,B]
 [fungal β-glucan] β-1,6-glucosidase (BT3314; BT_3314) 3.2.1.21   Bacteroides thetaiotaomicron VPI-5482 AAO78420.1
NP_812226.1		 Q8A2J1  
 exo-β-1,3-glucanase (BuGH3; BACUNI_01484) 3.2.1.58 pubmed
 Bacteroides uniformis ATCC 8492 ZP_02070067.1    
 β-glucosidase (Bgl8;BuBGL8) (partial) 3.2.1.21 pubmed
 Bacteroides uniformis ZL1 AGU13704.1 T1WEH3  
 β-glucosidase 3A (BaBgl3A; BAD_1194) (Bgl3A) 3.2.1.21 pubmed
 Bifidobacterium adolescentis ATCC 15703 BAF39975.1 A1A2P2 5WAB[A,B,C,D]
 β-glucosidase (BaBgl1A; BAD_1197) 3.2.1.21 pubmed
 Bifidobacterium adolescentis ATCC 15703 BAF39978.1    
 α-L-arabinopyranosidase / β-galactosidase (Apy;Apy-H1) 3.2.1.23
3.2.1.-		 pubmed
pubmed
 Bifidobacterium longum H-1 ADT80794.1 E7CY69  
 β-glucosidase (BglX) 3.2.1.21 pubmed
 Bifidobacterium longum H-1 ADY62498.1 F1DPQ8  
 β-glucosidase / β-xylosidase / multi-functional glycoside hydrolase (BGL;XYL;Blon_0625) 3.2.1.21
3.2.1.37
3.2.1.55		 pubmed
pubmed
pubmed
 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088 ACJ51732.1 B7GPK2  
 β-glucosidase (BlBG3; Bglx2; BLNIAS_00597) 3.2.1.21 pubmed
 Bifidobacterium longum subsp. longum KACC 91563 AEI96973.1 F8ATF7 5Z9S[A,B]
 β-glucosidase A (GluA; BpGluA; AW18_01575) 3.2.1.21 pubmed
 Bifidobacterium pseudocatenulatum IPLA36007 KEF29323.1    
 β-glucosidase B (GluB; BpGluB; AW18_09810) 3.2.1.21 pubmed
 Bifidobacterium pseudocatenulatum IPLA36007 KEF27912.1    
 β-glucosidase D (GluD; BpGluD; AW18_08145) 3.2.1.21 pubmed
 Bifidobacterium pseudocatenulatum IPLA36007 KEF28010.1    
 β-glucosidase E (GluE; BpGluE; AW18_08090 3.2.1.21 pubmed
 Bifidobacterium pseudocatenulatum IPLA36007 KEF28001.1    
 β-glucosidase (BpGH3-AR8;E5259_07130) 3.2.1.- pubmed
 Blautia producta DSM 2950 QMW77382.1    
 β-glucosidase (BpGH3-X62;E5259_15960) 3.2.1.- pubmed
 Blautia producta DSM 2950 QMW78964.1    
 β-hexosaminidase 1 (NagZ1; BcNagZ; BCAL2860) 3.2.1.52 pubmed
 Burkholderia cenocepacia J2315 CAR53159.1 A0A125HFC0
B4EA43		 4G6C[A,B]
4GNV[A,B]
4MSS[A,B]
5UTP[A,B]
5UTQ[A,B]
5UTR[A,B]
6DTE[A,B]
 β-glucosidase A (BglA) 3.2.1.21 pubmed
 Butyrivibrio fibrisolvens H17C AAA23008.1 P16084  
 β-xylosidase (ORF0541) (Xyl3A) 3.2.1.37 pubmed
 Caldanaerobius polysaccharolyticus KMCJ AFM44649.1 I6R6X6  
 β-xylosidase (Cb2487;Cb1172;CbXyl3A;Athe_2354) (Xyl3A) 3.2.1.37 pubmed
 Caldicellulosiruptor bescii DSM 6725 ACM61424.1 B9MN93  
 β-glucosidase (GH3-1) 3.2.1.21 pubmed
 Caldicellulosiruptor sp. F32 WP_039764154.1    
 β-glucosidase (GH3-2) 3.2.1.21 pubmed
 Caldicellulosiruptor sp. F32 WP_039765014.1    
 β-glucosidase / β-xylosidase (XynB5; CCNA_03149) 3.2.1.21
3.2.1.37		 pubmed
pubmed
 Caulobacter vibrioides NA1000 ACL96614.1 A0A0H3CBJ4  
 β-glucosidase (Cba) 3.2.1.21 pubmed
 Cellulomonas biazotea AAC38196.1 O51843  
 β-glucosidase (Cba5) 3.2.1.21   Cellulomonas biazotea ATCC 486 ASU50144.1    
 cellobiase / β-glucosidase (Cba2) 3.2.1.21 URL
 Cellulomonas biazotea ATCC 486 URH17144.1    
 bifunctional N-acetyl-β-glucosaminidase/β-glucosidase (Nag3) (acts as a phosphorylase in presence of phosphate) (Nag3A) 3.2.1.21
3.2.1.52
2.4.1.-
2.4.1.-		 pubmed
pubmed
pubmed
pubmed
 Cellulomonas fimi ATCC 484 AAQ05801.1 Q7WUL3  
 β-xylosidase (CfXyl3A;Celf_2726) (Xyl3A) 3.2.1.37 pubmed
 Cellulomonas fimi ATCC 484 AEE46850.1 F4H6T9  
 β-glucosidase (CfBgl3A;Celf_0140) (Bgl3A) 3.2.1.21 pubmed
 Cellulomonas fimi ATCC 484 AEE44289.1 F4H4T8  
 β-glycosidase (CfBgl3C;Celf_3372) (Bgl3C) 3.2.1.21 pubmed
 Cellulomonas fimi ATCC 484 AEE47485.1 F4H1R5  
 β-glucosidase (CfBgl3B;Celf_0468) (Bgl3B) 3.2.1.21 pubmed
 Cellulomonas fimi ATCC 484 AEE44608.1 F4H851  
 β-glucosidase (BglA) (fragment) 3.2.1.21 pubmed
 Cellulomonas flavigena CDBB531 / PN-120 AFP89333.1 J7H3I9  
 β-glucosidase (CgB) 3.2.1.21 pubmed
 Cellulomonas gilvus ATCC13127 BAA03152.1 P96316  
 gentiobiose β-1,6-glucosidase 3.2.1.- pubmed
 Cellulosimicrobium cellulans 21 UQT18186.1   8XRT[A,B,C,D,E,F]
8XRU[A,B,C]
8XRV[A,B,C,D,E,F]
8XRX[A,B,C,D,E,F]
 β-glucosidase (Bgl3A; CjGH3A; CJA_0204) (Cel3A) 3.2.1.58
3.2.1.97		   Cellvibrio japonicus Ueda107 ACE84847.1 B3PGE9  
 xyloglucan-specific exo-β-1,4-glucosidase (Blg3D; CjGH3D; CJA_1140) (Cel3D) 3.2.1.- pubmed
 Cellvibrio japonicus Ueda107 ACE82897.1 B3PBS6  
 β-glucosidase (Bgl3B; CjGH3B; CJA_1497) (Cel3B) 3.2.1.58
3.2.1.97
3.2.1.-		 pubmed
 Cellvibrio japonicus Ueda107 ACE83024.1 B3PDN7  
 β-glucosidase (Bgl3C; CjGH3C; CJA_0223) (Cel3C) 3.2.1.58 pubmed
 Cellvibrio japonicus Ueda107 ACE85841.1 B3PGG8  
 β-N-acetylglucosaminidase T (ChiT) 3.2.1.52 pubmed
 Chitiniphilus shinanonensis SAY3 BDB32681.1    
 β-glucosidase (Bgl; CsBGL) 3.2.1.21 pubmed
 Chryseobacterium scophthalmum 1433 QTA74163.1    
 β-glucosidase (CMM_0947) 3.2.1.21 pubmed
 Clavibacter michiganensis subsp. michiganensis NCPPB 382 CAN00986.1 A5CPI7  
 β-N-acetylglucosaminidase (Nag) (Nag3A) 3.2.1.52 pubmed
 Clostridium paraputrificum M-21 BAC56177.1 Q84IZ5  
 β-xylosidase (XylD) 3.2.1.37 pubmed
 Corynebacterium alkanolyticum Nakao et al. AJY53618.1 A0A0E3JDE2  
 β-glucosidase A (BglA; CHU_2268) 3.2.1.21 pubmed
 Cytophaga hutchinsonii ATCC 33406 ABG59531.1 A0A6N4STA7  
 β-glucosidase B (BglB; CHU_2273) 3.2.1.21 pubmed
 Cytophaga hutchinsonii ATCC 33406 ABG59535.1 A0A6N4SSX2  
 β-glucosidase / β-xylosidase (BgxA) 3.2.1.21
3.2.1.37		 pubmed
pubmed
 Dickeya chrysanthemi D1 AAA80156.1 Q46684  
 β-glucosidase (BgxA; Dda3937_03745) 3.2.1.21 pubmed
 Dickeya dadantii 3937 ADM98002.1    
 bifunctional β-glucosidase / β-xylosidase (Dth3; DICTH_1949) 3.2.1.21
3.2.1.37		   Dictyoglomus thermophilum H-6-12 ACI19278.1    
 β-xylosidase (Dtur_0852) 3.2.1.37 pubmed
 Dictyoglomus turgidum DSM 6724 ACK42133.1 B8E050  
 β-glucosidase / β-glycosidase (Dtur_0219) 3.2.1.21 pubmed
 Dictyoglomus turgidum DSM 6724 ACK41548.1
YP_002352162.1		 B8E1M3  
 β-glucosidase (Bgl;Fbgl;FmBGL) 3.2.1.21 doi
 Elizabethkingia meningoseptica AAB66561.1 O30713  
 β-glucosidase (BglX) 3.2.1.21 pubmed
 Escherichia coli str. K-12 substr. MG1655 AAA60495.1
AAB38487.1
AAC75193.1
AIZ90796.1
ALI40123.1
AMC95086.1
APC52371.1
AUG16880.1
AVI56630.1
AYG18842.1
NP_416636.1		 P33363  
 β-N-acetylglucosaminidase (NagZ; VcNagZ; YcfO) 3.2.1.52 pubmed
 Escherichia coli str. K-12 substr. MG1655 AAC74191.1
ACX49759.1
AIZ91775.1
ALI41114.1
AMC98318.1
AUG15890.1
AVI55625.1
AYG19803.1
BAA35922.1
NP_415625.1		 D0EX67
P75949		  
 β-glucosidase (FgBgl3A) (Bgl3A) 3.2.1.21 pubmed
 Fervidobacterium gondwanense AB39 AFY97405.1 K9URL4  
 Fisuc_2065 / FSU_2592 3.2.1.21 pubmed
doi
 Fibrobacter succinogenes subsp. succinogenes S85 ACX75652.1
ADL25584.1		 C9RJD5  
 β-glucosidase (BglF3;Fjoh_0775) 3.2.1.21 pubmed
 Flavobacterium johnsoniae UW101 ABQ03809.1 A5FLV3  
 β-glucosidase (BglBX10;Fjoh_3392) 3.2.1.21 pubmed
 Flavobacterium johnsoniae UW101 ABQ06406.1 A5FEF5  
 [oligo-ulvan] β-xylosidase (BN863_22130) 3.2.1.37 doi
 Formosa agariphila KMM 3901 CDF79925.1 T2KMH0  
 β-glucuronidase (BN863_22230) 3.2.1.31 pubmed
 Formosa agariphila KMM 3901 CDF79935.1 T2KMH9  
 β-glucosidase (GOTRE_010_00060) (possible fragment) 3.2.1.21 pubmed
 Gordonia terrae NBRC 100016 GAB42172.1    
 β-glycosidase (HaGH03; Haur_1431) 3.2.1.21 doi
 Herpetosiphon aurantiacus DSM 785 ABX04075.1 A9B3B2  
 β-N-acetylglucosaminidase (NagZ; BcNagZ; BF29_1739) 3.2.1.52 pubmed
 Heyndrickxia coagulans DSM 1 = ATCC 7050 AJH78617.1    
 β-xylosidase (BxyL) 3.2.1.37 pubmed
 Klebsiella sp. T4-1 AMK07469.1 A0A140D6H5  
 β-xylosidase (XylB) 3.2.1.37 pubmed
 Klebsiella sp. T5-5 AMK07510.1 A0A140D6K8  
 exo-β-1,4-glucosidase / β-glucosidase (β-Glu;G3ase) 3.2.1.21
3.2.1.74		 pubmed
pubmed
 Komagataeibacter sucrofermentans BPR2001 AAQ38005.1
BAA23595.1
BAA31467.1		 O24749  
 β-glucosidase (LVIS_1961) 3.2.1.21 pubmed
 Levilactobacillus brevis ATCC 367 ABJ65020.1 Q03P52  
 β-1,2-glucosidase / β-1,3-glucosidase (Lin1840) 3.2.1.58
3.2.1.-		 pubmed
pubmed
 Listeria innocua Clip11262 CAC97071.1
NP_471175.1		 Q92AS9 4ZO6[A,B]
4ZO7[A,B]
4ZO8[A,B]
4ZO9[A,B]
4ZOA[A,B]
4ZOB[A,B]
4ZOC[A,B]
4ZOD[A,B]
4ZOE[A,B]
 β-glucosidase (Gluc3M) 3.2.1.21 pubmed
 Martelella mediterranea gluc3m ADC53302.1 D3Y125  
 ginsenoside β-glucosidase (Bgp3) 3.2.1.21 pubmed
 Microbacterium esteraromaticum GS514 / KACC16318 AEX88467.1 H2DGJ4  
 ginsenoside β-glucosidase (Bgp1) 3.2.1.21 pubmed
 Microbacterium esteraromaticum KACC16318 AEX88466.1 H2DGJ3  
 [xanthan] β-glucosidase / d-4,5-unsaturated β-glucuronyl hydrolase (MiBgl3; AB663_001100) 3.2.1.21
3.2.1.-		 pubmed
pubmed
 Microbacterium sp. XT11 AMB17207.1
WP_067196459.1		    
 ginsenoside β-glucosidase (BglQM;Bgl-QM49-1) 3.2.1.21 pubmed
 Mucilaginibacter sp. QM49 AFS34656.1 J9Y3X3  
 β-N-acetylglucosaminidase (Rv0237;LpqI) 3.2.1.52 pubmed
 Mycobacterium tuberculosis H37Rv CAA17329.1
AFN48090.1
CAE55256.1
CCP42966.1
NP_214751.1		 L7N6B0 6GFV[A,B]
 β-glucosidase-767 (A8C56_22685) 3.2.1.- pubmed
 Niabella ginsenosidivorans BS26 ANH83415.1    
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