Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-); β-1,2-glucosidase (EC 3.2.1.-); β-1,3-glucosidase (EC 3.2.1.-); xyloglucan-specific exo-β-1,4-glucanase / exo-xyloglucanase (EC 3.2.1.155); stevioside-β-1,2-glucosidase (EC 3.2.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); protodioscin 26-O-β-D-glucosidase (EC 3.2.1.186); β-glucuronidase (EC 3.2.1.31); avenacinase (EC 3.2.1.-); tomatinase β-1,2-glucosidase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that some enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (48397); Uniprot accession (325); PDB accession (181); 3D entries (54); cryst (4)
| 1 | 2 | 3 | 4 |
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 β-xylosidase (XynB3;Theth_0138) 3.2.1.37 pubmed
Pseudothermotoga thermarum DSM 5069 AEH50242.1 F7YUQ7  
 β-N-acetylhexosaminidase (RRR_05655) 3.2.1.52 pubmed
Rickettsia rickettsii str. R AJG33435.1    
 xylan β-1,4-xylosidase (RiXyl3A;ROSINTL182_08202) (Xyl3A) 3.2.1.37 pubmed
Roseburia intestinalis L1-82 EEU99897.1 C7GE50  
 β-glucosidase (RiGH3B; RIL182_00213; ROSINTL182_07684; Bglb_2) 3.2.1.21 pubmed
Roseburia intestinalis L1-82 VCV20358.1
EEV00398.1
C7GCP6  
 β-glucosidase (RiGH3A; RIL182_00212; ROSINTL182_07685; Bglb_1) 3.2.1.21 pubmed
Roseburia intestinalis L1-82 VCV20357.1
EEV00399.1
C7GCP7  
 β-glucosidase 3A (Ccel_2454) (Glu3A) 3.2.1.21 pubmed
Ruminiclostridium cellulolyticum H10 ACL76783.1    
 β-glucosidase (GluA) 3.2.1.21 URL
Ruminococcus albus 7 AAC05445.1 O66050  
 β-xylosidase 3A (Xyl3A) 3.2.1.37 pubmed
Ruminococcus albus 8 AEE64772.1
ZP_08159508.1
F6LP84  
 β-1,3-glucosidase (Ra1595;CUS_7631) 3.2.1.- pubmed
Ruminococcus albus 8 EGC01435.1
ZP_08160757.1
E9SHA3  
 β-glucosidase (Bgl) 3.2.1.21 pubmed
Ruminococcus albus ATCC27210 CAA33461.1 P15885  
 β-glucosidase / cellodextrinase 3B (Sde_0245) (Ced3B) 3.2.1.21
3.2.1.74
pubmed
pubmed
Saccharophagus degradans 2-40 ABD79509.1 Q21P70  
 β-glucosidase / cellodextrinase 3A (CedA;Sde_2497) (Ced3A) 3.2.1.21
3.2.1.74
pubmed
pubmed
Saccharophagus degradans 2-40 ABD81757.1 Q21HS2  
 β-glucosidase 3C (Sde_2674) (Bgl3C) 3.2.1.21 pubmed
Saccharophagus degradans 2-40 ABD81934.1 Q21H95  
 erythromycin β-glucosidase (EryBI;SACE_0732) 3.2.1.21 pubmed
Saccharopolyspora erythraea NRRL 2338 CAA74702.1
CAM00073.1
A4F7P9
O70021
 
 β-N-acetylglucosaminidase (NagZ;STM1209) 3.2.1.52 pubmed
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 AAL20138.1
NP_460179.1
Q8ZQ06 4GVF[A,B]
4GVG[A,B]
4GVH[A,B]
4GVI[A,B]
4HZM[A,B]
 β-glucosidase (BglSk;Sked_04360) 3.2.1.21 pubmed
Sanguibacter keddieii DSM 10542 ACZ20402.1 D1BKC5  
 β-glucosidase (BglA49) 3.2.1.21 pubmed
Serratia sp. TN49 ADK91094.1 G0TEQ0  
 endo-β-1,3(4)-glucanase (STHERM_c13940) 3.2.1.6 pubmed
Spirochaeta thermophila DSM 6192 ADN02334.1 E0RUB3  
 oleandomycin β-glycosidase (OleR) 3.2.1.21 pubmed
Streptomyces antibioticus ATCC 11891 AAC12650.1 O68843  
 β-N-acetylhexosaminidase (NagA;SCO2758) 3.2.1.52 pubmed
Streptomyces coelicolor A3(2) CAB66297.1
NP_626989.1
Q9RDG9  
 β-N-acetylglucosaminidase (DasD;SCO5235) 3.2.1.52 pubmed
Streptomyces coelicolor A3(2) CAB94620.1
NP_629382.1
Q9K488  
 β-xylosidase (Bxl2) 3.2.1.37 doi
Streptomyces sp. CH7 AAO42605.1 Q7X508  
 β-N-acetylglucosaminidase II (NagA) 3.2.1.52 pubmed
Streptomyces thermoviolaceus OPC-520 BAA32403.1 O82840  
 β-xylosidase (BxlA) 3.2.1.37 pubmed
Streptomyces thermoviolaceus OPC-520 BAB61064.1
BAD02389.1
Q76BU6 cryst
 macrolide β-glycosidase / β-glucosidase (DesR) 3.2.1.21 pubmed
Streptomyces venezuelae ACR54627.1
AAC68679.1
AAO96653.1
AAO98875.1
AAY77317.1
C5IXN7
Q9ZGH5
4I3G[A,B]
 β-N-acetylhexosaminidase (NahA; STH300) 3.2.1.52 doi
Symbiobacterium thermophilum IAM 14863 BAD39285.1 Q67SQ8  
 3-O-β-glucopyranosyl-specific ginsenosidase type III / β-glycosidase (BgpA;Bgl-gyp 3082) 3.2.1.21
3.2.1.38
3.2.1.-
pubmed
pubmed
pubmed
Terrabacter ginsenosidimutans ACZ66247.2
ACZ66247.1
D2KAT1  
 β-xylosidase / α-L-arabinofuranosidase (XarB) 3.2.1.37
3.2.1.55
pubmed
pubmed
Thermoanaerobacter ethanolicus JW 200 AAF43783.1
AAB23220.1
Q9JRQ1  
 cellodextrinase D (BglA;Ggh-A) 3.2.1.74 pubmed
Thermobispora bispora NRRL 15568 AAB36835.1 Q59506  
 β-xylosidase B (BxlB) (Bxl3B) 3.2.1.37 pubmed
Thermoclostridium stercorarium NCIMB 11745 CAD48309.1 Q8GJ42  
 β-glucosidase Z (BglZ) (Bgl3Z) 3.2.1.21 pubmed
Thermoclostridium stercorarium NCIMB 11754 CAB08072.1 O08331  
 β-xylosidase 3B (Bxl3B; BxlB; Cst_c02510) 3.2.1.37 pubmed
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 AGI38341.1
AGC67275.1
   
 β-glucosidase 3Z (Bgl3Z; BglZ; Cst_c03130) 3.2.1.21 pubmed
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 AGC67337.1
AGI38398.1
   
 low activity β-N-acetylglucosaminidase / β-glucuronidase 3A (Nag3A; Cst_c13470) 3.2.1.31
3.2.1.52
pubmed
pubmed
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 AGI39362.1
AGC68338.1
   
 β-glucosidase B (BglB;TM0025) (Cel3) 3.2.1.21 doi
Thermotoga maritima MSB8 AAD35119.1
AAM56786.1
AGL48948.1
AHD18204.1
AKE27834.1
CAH68687.1
CAT81443.1
NP_227841.1
Q9WXN2
R4NZC1
 
 β-xylosidase (XloA;TM0076) (Xyl3) 3.2.1.37 pubmed
Thermotoga maritima MSB8 AAD35170.1
AGL48999.1
AHD18154.1
AKE27884.1
NP_227892.1
Q9WXT1
R4NX63
cryst
 β-N-acetylglucosaminidase (NagA;TM0809) 3.2.1.52 pubmed
Thermotoga maritima MSB8 AAD35891.1
AGL49735.1
AHD17438.1
AKE28590.1
NP_228618.1
B6DVV2
G4FD15
Q9WZR6
3WO8[A,B]
 β-glucosidase B (BglB;TnBgl3B;CTN_0670) (Bgl3B) 3.2.1.21 pubmed
Thermotoga neapolitana DSM 4359 ABI29899.1
ACM22846.1
B9K7B3
Q0GC07
2WT3[A]
2WT5[A]
2WT6[A]
2X40[A]
2X41[A]
2X42[A]
 β-N-acetylglucosaminidase (CbsA) 3.2.1.52 pubmed
Thermotoga neapolitana KCCM41025 AAK16587.1 Q9AG27 5BZA[A,B,C,D]
5C0Q[A,B,C,D]
 β-glucosidase B / laminaribiase (BglB) 3.2.1.21
3.2.1.-
pubmed
pubmed
Thermotoga neapolitana Z2706-MC24 CAB01407.1 Q60038 cryst
 β-xylosidase (TpeXyl3 ; Tpet_0848) 3.2.1.37 pubmed
Thermotoga petrophila RKU-1 ABQ46867.1    
 β-glucosidase (BG-B;TCel11;TpBlg3;Tpet_0898) 3.2.1.21 pubmed
Thermotoga petrophila RKU-1 ABQ46916.1 A5IL43  
 exo-chitosanase (Cho) 3.2.1.132 pubmed
Trichormus fertilissimus RPAN1 ACV53814.1 C9D7S2  
 β-glucoside phosphorylase (BglP) 2.4.1.- pubmed
uncultured bacterium AUG44408.1
AUG44422.1
A0A2H4ZRM6
A0A2R2JFS5
5VQD[A]
5VQE[A]
 β-glucosidase (Bgl2;BGL4A6-ORF17) 3.2.1.21 pubmed
uncultured bacterium AEV59721.1 G9BYA2  
 β-glucosidase (Bgl1;BGL02B4-ORF21) 3.2.1.21 pubmed
uncultured bacterium AEV59720.1 G9BYA1  
 β-glucosidase (UmBgl3B;RG2) 3.2.1.21 pubmed
uncultured bacterium ABA42186.1 Q3HXC2  
 β-glucosidase (UmBgl3C;RG3) 3.2.1.21 pubmed
uncultured bacterium ABA42187.1 Q3HXC1  
 β-glucosidase (BGL5;Bgl7O16) 3.2.1.21 pubmed
uncultured bacterium AFH08277.1 H9XVR0  
 β-glucosidase (BGL7;Bgl9) 3.2.1.21 pubmed
uncultured bacterium AFH08279.1 H9XVR2  
 β-glucosidase (BGL9;Bgl11) 3.2.1.21 pubmed
uncultured bacterium AFH08281.1 H9XVR4  
 β-glucosidase (UmBgl3D) 3.2.1.21 pubmed
uncultured bacterium ABE60716.1 Q1PDD6  
 β-glucosidase (RG11) 3.2.1.21 pubmed
uncultured bacterium ABI94087.1 Q06B36  
 β-glucosidase (RG12) 3.2.1.21 pubmed
uncultured bacterium ABI94088.1 Q06B35  
 β-glucosidase (RG14) 3.2.1.21 pubmed
uncultured bacterium ABI94089.1 Q06B34  
 β-glucosidase (RG20) 3.2.1.21 pubmed
uncultured bacterium ABI94090.1 Q06B33  
 β-glucosidase (RG25) 3.2.1.21 pubmed
uncultured bacterium ABI94091.1 Q06B32  
 β-glucosidase (AS-Esc6) 3.2.1.21 pubmed
uncultured bacterium AHG23300.1 W0LMV0  
 β-glucosidase (AmBGL17) 3.2.1.21 pubmed
uncultured bacterium AHI85741.1 W6CGK7  
 β-glucosidase (AS-Esc17) 3.2.1.21 pubmed
uncultured bacterium AIF34069.1 A0A075J216  
 β-glucosidase (A1_GH3) 3.2.1.21 pubmed
uncultured bacterium A1_9 AEW47937.1 G9IS15  
 β-glucosidase 3.2.1.21 pubmed
uncultured bacterium Contig178 AHF25279.1 W0FP77  
 β-glucosidase (D1_GH3) 3.2.1.21 pubmed
uncultured bacterium D1_14 AEW47953.1 G9IS30  
 β-glucosidase (H1_GH3) 3.2.1.21 pubmed
uncultured bacterium H1_5 AEW47970.1 G9IS47  
 β-glucosidase (LAB20g4) 3.2.1.21 pubmed
uncultured bacterium LAB20 AGH13484.1 M4Q7P2  
 glucan β-1,4-glucosidase (LAB25g2) 3.2.1.21 pubmed
uncultured bacterium LAB25 AGH13451.1 M4Q070  
 β-glucosidase (SRF2g14) 3.2.1.21 pubmed
uncultured bacterium SRF2 AGH13471.1 M4Q086  
 glucan 1,4-β-glucosidase (SRF2g18) 3.2.1.21 pubmed
uncultured bacterium SRF2 AGH13475.1 M4PZB2  
 β-glucosidase/β-xylosidase (Bgx1;RuBGX1) 3.2.1.21
3.2.1.37
pubmed
pubmed
uncultured rumen bacterium ADD17009.1 D3VYD8  
 β-glucosidase/β-xylosidase (RuBg3B;Bgx3;RuBbgX3;3F8) 3.2.1.21
3.2.1.37
pubmed
pubmed
uncultured rumen bacterium ADM89626.1 G3BJX4  
 β-glucosidase/β-xylosidase (RuBg3A;BgX2;RubgX2;16C12) 3.2.1.21
3.2.1.37
pubmed
pubmed
uncultured rumen bacterium ADM89625.1 G3BJX3  
 β-xylosidase (XynA;R13) (Xyl3A) 3.2.1.37 pubmed
uncultured rumen bacterium CAP07659.1 A8E1A9  
 β-glucosidase (CelB) 3.2.1.21 pubmed
unidentified bacterium AAA91967.1 Q60055  
 β-glucosidase (LamN; A1Q_4052) 3.2.1.21 pubmed
Vibrio campbellii HY01 EDL69935.1    
 β-N-acetylhexosaminidase (NagZ;VC0692) 3.2.1.52 pubmed
Vibrio cholerae O1 biovar El Tor str. N16961 AAF93857.1
NP_230341.1
Q9KU37 1TR9[A]
1Y65[A]
2OXN[A]
3GS6[A]
3GSM[A]
  β-N-acetylglucosaminidase II (EXoII) 3.2.1.52 pubmed
Vibrio furnissii AAC44686.2
AAE36541.1
P96157  
 [N-glycan] β-1,2-xylosidase (NixI;XCC2892) 3.2.1.37 pubmed
Xanthomonas campestris pv. campestris str. ATCC 33913 AAM42164.1
NP_638240.1
Q8P6S3  
 exo-β-1,4-glucanase (XAC1448;BglS) 3.2.1.74 doi
Xanthomonas citri pv. citri str. 306 AAM36318.1
NP_641782.1
Q8PMI0  
 exo-β-1,4-glucanase (XAC1793;CelD) 3.2.1.74 doi
Xanthomonas citri pv. citri str. 306 AAM36656.1
NP_642120.1
Q8PLK4  
 β-xylosidase (XAC3076) 3.2.1.37 doi
Xanthomonas citri pv. citri str. 306 AAM37921.1
NP_643385.1
Q8PI22  
 exo-β-1,4-glucanase (XAC3869;BglX) 3.2.1.74 doi
Xanthomonas citri pv. citri str. 306 AAM38711.1
NP_644175.1
Q8PFV3  
 β-xylosidase (XAC4231) 3.2.1.37 doi
Xanthomonas citri pv. citri str. 306 AAM39066.1
NP_644530.1
Q8PEV9  
Eukaryota
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 α-L-arabinofuranosidase / β-1,4-xylosidase (Xyl3;AtBX3;At5g09730) 3.2.1.37
3.2.1.55
pubmed
pubmed
Arabidopsis thaliana CAB89357.1
AAK96639.1
AED91439.1
BAB09531.1
CAL81217.1
CAW52616.1
CBC23860.1
NP_196535.1
Q9LXD6  
 α-L-arabinofuranosidase / β-D-xylosidase (Xyl1;BXL1;AtBXL1;At5g49360) 3.2.1.37
3.2.1.55
pubmed
pubmed
Arabidopsis thaliana BAB09906.1
AAM53325.1
AED95802.1
BAF00595.1
CAW52618.1
CAW96520.1
CAW96683.1
CAX01897.1
CAX07251.1
CBC23864.1
CBD30887.1
NP_199747.1
Q0WL73
Q0WQK4
Q9FGY1
 
 β-xylosidase (Xyl4;AtBXL4;At5g64570) 3.2.1.37 pubmed
Arabidopsis thaliana BAB11424.1
AED97922.1
ANM70278.1
ANM70279.1
BAD94481.1
CAW52619.1
CBC23866.1
NP_201262.1
A0A178UTL8
A0A1P8BF93
A0A1P8BFA0
Q9FLG1
 
 β-glucosidase 1 (Bgl1;AaBGL1;AaBg1;AabG) 3.2.1.21 pubmed
Aspergillus aculeatus F-50 BAA10968.1
AER93027.1
G8GBT7
P48825
4IIB[A,B]
4IIC[A,B]
4IID[A,B]
4IIE[A,B]
4IIF[A,B]
4IIG[A,B]
4IIH[A,B]
 β-xylosidase (Xyl) 3.2.1.37 pubmed
Aspergillus awamori K4 BAE19756.1
CAW52633.1
CBC23895.1
Q4AEG8  
 β-xylosidase (Xylx100) 3.2.1.37 pubmed
Aspergillus awamori X-100 ACF61038.1 B5AK91
Q4AEG8
 
 β-glucosidase (Bgl1;NfBGL1) 3.2.1.21 pubmed
Aspergillus fischeri P1 / CGMCC 3.15369 AIM56897.1 A0A096Y6Z0  
 β-glucosidase (AfG;AFUA_8G02100) 3.2.1.21 pubmed
Aspergillus fumigatus Af293 EAL84958.1 Q4WBF4  
 β-glucosidase (AfBG;AFUA_1G17410;Afu1g17410) 3.2.1.21 pubmed
Aspergillus fumigatus Af293 EAL91070.1 Q4WR62  
 β-glucosidase 1 (Afum-Bgl101;AfbG;AFUA_1G05770;Afu1g05770) 3.2.1.21 pubmed
Aspergillus fumigatus Af293 EAL88289.1
ABU35789.1
ACE10248.1
ACH05254.1
ACJ91428.1
Q4WJJ3 5FJI[A,B]
 β-glucosidase / cellulase (Bgl3) 3.2.1.21 pubmed
Aspergillus fumigatus Z5 ADX78143.1 F1DGF3  
 β-1,4-xylosidase (XylA) 3.2.1.37 pubmed
Aspergillus japonicus MU-2 BAG82824.1 B6EY09  
 β-glucosidase A (BglA) 3.2.1.21 pubmed
Aspergillus kawachii IFO4308 BAA19913.1 P87076  
 β-xylosidase (XlnD) 3.2.1.37 pubmed
Aspergillus nidulans CAA73902.1 O42810
Q5BAS1
 
 β-glucosidase (AN1804.2) 3.2.1.21 pubmed
Aspergillus nidulans FGSC A4 EAA64969.1 Q5BCC6  
 β-xylosidase (BxlB;AnXA;AN8401.2) 3.2.1.37 pubmed
Aspergillus nidulans FGSC A4 EAA67023.1 Q5ATH9  
 β-xylosidase (BxlA;XlnD;AN2359.2) 3.2.1.37 pubmed
Aspergillus nidulans FGSC A4 EAA64470.1 Q5BAS1 6Q7I[A,B]
6Q7J[A,B]
 β-glucosidase (AN0712.2) 3.2.1.21 pubmed
Aspergillus nidulans FGSC A4 EAA65189.1
ABF50843.1
Q5BFG8  
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