Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-); β-1,2-glucosidase (EC 3.2.1.-); β-1,3-glucosidase (EC 3.2.1.-); xyloglucan-specific exo-β-1,4-glucanase / exo-xyloglucanase (EC 3.2.1.155); stevioside-β-1,2-glucosidase (EC 3.2.1.-); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); protodioscin 26-O-β-D-glucosidase (EC 3.2.1.186); β-glucuronidase (EC 3.2.1.31); avenacinase (EC 3.2.1.-); tomatinase β-1,2-glucosidase (EC 3.2.1.-)
Mechanism Retaining
3D Structure Status( β / α ) 8 barrel
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that some enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (47963); Uniprot accession (325); PDB accession (181); 3D entries (54); cryst (4)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-glucosidase B (BglB;Cthe_1256) 3.2.1.21 Acetivibrio thermocellus ATCC 27405 ABN52488.1 P14002
7MS2[A]
 β-N-acetylhexosaminidase (Amuc_2109) 3.2.1.52 Akkermansia muciniphila ATCC BAA-835 ACD05918.1
QTD80614.1
 
7VI6[A] 2.00
7VI7[A] α-D-GlcpNAc
2.00
 β-glucosidase EmGH1   Aurantiacibacter marinus    
5Z87[B] 2.30
5Z87[A] 2.30
 β-N-acetylglucosaminidase (NagZ;YbbD;YzbA;BSU01660) 3.2.1.52 Bacillus subtilis subsp. subtilis str. 168 AAA64351.1
AIY91451.1
AQR80107.1
AQR84320.1
BAA19499.1
CAB11942.1
NP_388047.1
A0A6M4JEW2
P40406
3BMX[A,B] 1.40
3CQM[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
1.70
3LK6[A,B,C,D] 2.88
3NVD[A,B] α-D-Glcp1N2NAc-(1-1)-<non_carb>
1.84
4GYJ[A,B] β-D-GlcpNAc-(1-4)[<non_carb>-(1-3)]-α-D-GlcpNAc
1.65
4GYK[A,B] β-D-GlcpNAc-(1-4)[<non_carb>-(1-3)]-α-D-GlcpNAc
1.80
 BACINT_00768   Bacteroides intestinalis DSM 17393 EDV07201.1  
5TF0[A,B] 2.20
 β-glucosidase (Bglx_5;BoGH3B;BACOVA_02659;Bovatus_03075) 3.2.1.21 Bacteroides ovatus ATCC 8483 ALJ47683.1 A7LXU3
5JP0[A,B] β-D-Glcp
2.30
 β-1,2-glucosidase (BT3567) 3.2.1.- Bacteroides thetaiotaomicron VPI-5482 AAO78673.1
NP_812479.1
Q8A1U1
5XXL[A,B] 1.60
5XXM[A,B] β-D-Glcp
1.70
5XXN[A,B] α-D-Glcp
β-D-Glcp-(1-2)-β-D-Glcp
2.05
5XXO[A,B] β-D-Glcp
β-D-Glcp-(1-2)-β-D-Glcp-(1-2)-β-D-Glcp
2.02
 β-N-acetylhexosaminidase (Bcav_3777)   Beutenbergia cavernae DSM 12333 ACQ82019.1  
5BU9[A,B] 2.26
 β-glucosidase (BAD_1194) 3.2.1.21 Bifidobacterium adolescentis ATCC 15703 BAF39975.1 A1A2P2
5WAB[A,B,C,D] 2.45
 β-glucosidase (BLNIAS_00597; Bglx2) 3.2.1.21 Bifidobacterium longum subsp. longum KACC 91563 AEI96973.1 F8ATF7
5Z9S[A,B] β-D-Glcp
2.30
 BCN122_I0864 (NagZ) 3.2.1.52 Burkholderia cenocepacia GIMC4560:Bcn122 ARF84251.1 A0A125HFC0
6DTE[A,B] 1.93
 β-hexosaminidase 1 (NagZ1;BCAL2860)   Burkholderia cenocepacia J2315 CAR53159.1  
4G6C[A,B] 1.38
4GNV[A,B] β-D-GlcpNAc
1.50
4MSS[A,B] 1.80
5UTP[A] <non_carb>-(1-2)-α-D-Glcp1NN-(1-1)-<non_carb>
2.20
5UTQ[A] α-D-Glcp1N2NAc-(1-1)-<non_carb>
β-D-Glcp1N2NAc-(1-1)-<non_carb>
2.20
5UTR[A] 2.15
 cg3158 (NagA2)   Corynebacterium glutamicum ATCC 13032 BAC00246.1
CAF20877.1
CCH25974.1
NP_602044.1
 
5IOB[A,B,C,D,E,F,G,H] 2.25
 DR1333 / DR_1333   Deinococcus radiodurans R1 AAF10903.1
ANC72728.1
NP_295057.1
 
3TEV[A,B] 2.30
 β-1,2-glucosidase / β-1,3-glucosidase (Lin1840) 3.2.1.-
3.2.1.-
Listeria innocua Clip11262 CAC97071.1
NP_471175.1
Q92AS9
4ZO6[A,B] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
2.00
4ZO7[A,B] α-D-Glcp
β-D-Glcp
β-D-Glcp-(1-6)-α-D-Glcp
2.00
4ZO8[A,B] β-D-Glcp-(1-2)-β-D-Glcp
2.00
4ZO9[A,B] β-D-Glcp-(1-3)-β-D-Glcp
1.99
4ZOA[A,B] <carb_like_IFM>
2.17
4ZOB[A,B] β-D-Glcp
2.40
4ZOC[A,B] β-D-Glcp
1.79
4ZOD[A,B] β-D-Glcp
2.10
4ZOE[A,B] 1.80
 β-N-acetylglucosaminidase (Rv0237;LpqI) 3.2.1.52 Mycobacterium tuberculosis H37Rv CAA17329.1
AFN48090.1
CAE55256.1
CCP42966.1
NP_214751.1
L7N6B0
6GFV[A,B] 1.96
 MSMEG_0361   Mycolicibacterium smegmatis MC2 155 ABK70873.1
AFP36835.1
AIU05638.1
 
4YYF[A,B,C] 1.92
 NGO_0135   Neisseria gonorrhoeae FA 1090 AAW88893.1  
6JTI[A,B,C,D,E,F] 2.20
6JTJ[A,B,C,D,E,F] β-D-GlcpNAc
2.18
6JTK[A,B,C,D,E,F] <non_carb>-(1-2)-β-D-GlcpN
2.20
6JTL[A,B] 2.40
 β-glucosidase   Paenibacillus barengoltzii CAU904 APZ77041.1  
5WUG[A] 2.22
5WVP[A] β-D-Manp
2.29
  β-N-acetylglucosaminidase (PsNagA) 3.2.1.52 Paenibacillus sp. FPU-7 BBK60996.1 A0A5S8WF56
6K5J[A] β-D-GlcpNAc
1.90
 exo-1,3/1,4-β-glucanase (GluA;ExoP) 3.2.1.- Pseudoalteromonas sp. BB1 ABC88234.1 Q0QJA3
3F93[A,B,C,D] 2.45
3F94[A] 2.30
3RRX[A] 1.90
3USZ[A] 2.10
3UT0[A,B,C,D] 2.30
 β-N-acetylglucosaminidase (NagZ;PA3005) 3.2.1.52 Pseudomonas aeruginosa PAO1 AAG06393.1
NP_251695.1
Q9HZK0
5G1M[A,B] 1.80
5G2M[A,B] β-D-GlcpNAc
3.00
5G3R[A,B] β-D-GlcpNAc
2.18
5G5K[A,B] <carb_like_NOK>
3.10
5G5U[A,B] 2.25
5G6T[A,B] 2.15
5LY7[A,B] <carb_like_NOK>
3.10
 PA1726 (BglX) 3.2.1.-
3.2.1.-
Pseudomonas aeruginosa PAO1 AAG05115.1
NP_250417.1
Q9I311
6R5I[A,B] 1.80
6R5N[A,B] <carb_like_NOJ>
2.00
6R5O[A,B] β-D-Glcp
2.40
6R5P[A,B] β-D-Glcp
2.85
6R5R[A,B] β-D-Glcp-(1-4)-β-D-Glcp
1.65
6R5T[A,B] β-D-Galp-(1-4)-β-D-Glcp
1.60
6R5U[A,B] β-D-Glcp-(1-3)-β-D-Glcp
β-D-Glcp-(1-3)-β-D-Glcp-(1-3)-β-D-Glcp
2.15
6R5V[A,B] β-D-Xylp-(1-4)-β-D-Xylp
1.80
 glucan 1,4-β-glucosidase (SACE_6502)   Saccharopolyspora erythraea NRRL 2338 CAM05671.1  
5M6G[A] 1.83
 β-N-acetylglucosaminidase (NagZ;STM1209) 3.2.1.52 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 AAL20138.1
NP_460179.1
Q8ZQ06
4GVF[A,B] α-D-GlcpNAc
1.35
4GVG[A,B] 1.70
4GVH[A,B] α-D-GlcpNAc-(1-4)-ASP
1.45
4GVI[A,B] 1.55
4HZM[A,B] 1.45
 β-xylosidase (BxlA) 3.2.1.37 Streptomyces thermoviolaceus OPC-520 BAB61064.1
BAD02389.1
Q76BU6
cryst    
 macrolide β-glycosidase / β-glucosidase (DesR) 3.2.1.21 Streptomyces venezuelae ACR54627.1
AAC68679.1
AAO96653.1
AAO98875.1
AAY77317.1
C5IXN7
Q9ZGH5
4I3G[A,B] β-D-Glcp
1.40
 SYNPCC7002_A0075   Synechococcus sp. PCC 7002 ACA98092.1  
3SQL[A,B] 2.25
3SQM[A,B,C,D] β-D-GlcpNAc
2.70
 β-xylosidase (XloA;TM0076) (Xyl3) 3.2.1.37 Thermotoga maritima MSB8 AAD35170.1
AGL48999.1
AHD18154.1
AKE27884.1
NP_227892.1
Q9WXT1
R4NX63
cryst    
 β-N-acetylglucosaminidase (NagA;TM0809) 3.2.1.52 Thermotoga maritima MSB8 AAD35891.1
AGL49735.1
AHD17438.1
AKE28590.1
NP_228618.1
B6DVV2
G4FD15
Q9WZR6
3WO8[A,B] 2.43
 β-glucosidase B (BglB;TnBgl3B;CTN_0670) (Bgl3B) 3.2.1.21 Thermotoga neapolitana DSM 4359 ABI29899.1
ACM22846.1
B9K7B3
Q0GC07
2WT3[A] β-D-Glcp
2.05
2WT5[A] α-D-Glcp
2.10
2WT6[A] 2.31
2X40[A] 2.31
2X41[A] β-D-Glcp
2.05
2X42[A] α-D-Glcp
2.099
 β-N-acetylglucosaminidase (CbsA) 3.2.1.52 Thermotoga neapolitana KCCM41025 AAK16587.1 Q9AG27
5BZA[A,B,C,D] 2.00
5C0Q[A,B,C,D] 2.50
 β-glucosidase B / laminaribiase (BglB) 3.2.1.21
3.2.1.-
Thermotoga neapolitana Z2706-MC24 CAB01407.1 Q60038
cryst    
 β-glucoside phosphorylase (BglP) 2.4.1.- uncultured bacterium AUG44408.1
AUG44422.1
A0A2H4ZRM6
A0A2R2JFS5
5VQD[A] 2.10
5VQE[A] α-D-Glcp2F-(1-4)-ASP
1.89
 β-N-acetylhexosaminidase (NagZ;VC0692) 3.2.1.52 Vibrio cholerae O1 biovar El Tor str. N16961 AAF93857.1
NP_230341.1
Q9KU37
1TR9[A] 1.80
1Y65[A] α-D-GlcpNAc
1.85
2OXN[A] β-D-Glcp1N2NAc-(1-1)-<non_carb>
1.70
3GS6[A] <non_carb>-(1-2)-β-D-Glcp1NN-(1-1)-<non_carb>
2.30
3GSM[A] <non_carb>-(1-2)-β-D-Glcp1NN
2.40
Eukaryota
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-glucosidase 1 (Bgl1;AaBGL1;AaBg1;AabG) 3.2.1.21 Aspergillus aculeatus F-50 BAA10968.1
AER93027.1
G8GBT7
P48825
4IIB[A,B] 1.80
4IIC[A,B] <carb_like_IFM>
1.90
4IID[A,B] <carb_like_NOJ>
2.30
4IIE[A,B] <carb_like_CGB>
2.00
4IIF[A,B] <carb_like_CTS>
2.45
4IIG[A,B] β-D-Glcp
2.30
4IIH[A,B] β-D-Glcp-(1-4)-β-D-Glcp4S
2.00
 β-glucosidase 1 (Afum-Bgl101;AfbG;AFUA_1G05770;Afu1g05770) 3.2.1.21 Aspergillus fumigatus Af293 EAL88289.1
ABU35789.1
ACE10248.1
ACH05254.1
ACJ91428.1
Q4WJJ3
5FJI[A,B] 1.95
 β-xylosidase (BxlA;XlnD;AN2359.2) 3.2.1.37 Aspergillus nidulans FGSC A4 EAA64470.1 Q5BAS1
6Q7I[A,B] 1.48
6Q7J[A,B] 2.14
 β-glucosidase (B7;BglA;Bgl1;AobG;BGHG1;BglC;AO090009000356) (Cel3A) 3.2.1.21 Aspergillus oryzae RIB40 BAE54829.1 Q2UUD6
5FJJ[A,B,C,D] 1.95
 isoprimeverose-producing oligoxyloglucan hydrolase (AO090701000274; IpeA; BglD) 3.2.1.120 Aspergillus oryzae RIB40 BAE62006.1  
5YOT[A,B] 1.98
5YQS[A,B] α-D-Xylp-(1-6)-α-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp
β-D-Glcp
2.40
7EAP[A] α-D-Xylp-(1-6)-β-D-Glcp
α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp
1.42
 β-glucosidase (D2-BGL) 3.2.1.21 Chaetomella raphigera 737 AID68373.1 A0A068FT77
6JXG[A] 1.90
 exo-β-1,3-1,4-glucanase (ExoI) 3.2.1.- Hordeum vulgare subsp. vulgare AAD23382.1
BAK05680.1
F2EEA8
Q9XEI3
1EX1[A] α-D-Glcp-(1-4)-ASP
2.20
1IEQ[A] β-D-Glcp
2.70
1IEV[A] <carb_like_INS>
2.80
1IEW[A] α-D-Glcp2F-(1-4)-ASP
2.55
1IEX[A] β-D-Glcp-(1-4)-β-D-Glcp4S
2.20
1J8V[A] β-D-Glcp-(1-3)-β-D-Glcp3S
2.40
1LQ2[A] <carb_like_IDD>
2.70
1X38[A] <carb_like_IDD>
1.70
1X39[A] <carb_like_IDE>
1.80
3WLH[A] β-D-Glcp
1.65
3WLI[A] 1.45
3WLJ[A] β-D-3-deoxy-Glcp
β-D-Glcp
1.67
3WLK[X] β-D-Glcp
1.80
3WLL[A] 1.80
3WLM[A] β-D-Glcp-(1-1)-octyl
1.90
3WLN[A] β-D-Glcp-(1-1)-octyl
2.00
3WLO[A] β-D-Glcp
1.55
3WLP[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.57
3WLQ[A] 1.65
3WLR[A] 2.21
3WLS[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
2.30
3WLT[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.98
6JG1[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp1SH
1.78
6JG2[A] β-D-Glcp-(1-3)-α-L-Allp-(1-1)-<non_carb>
2.00
6JG6[A] 1.55
6JG7[A] β-D-Glcp-(1-2)-β-D-Glcp-(1-1)-methyl
2.16
6JGA[A] β-D-Glcp-(1-3)-β-D-Glcp3S
1.47
6JGB[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.47
6JGC[A] α-D-Glcp
2.36
6JGD[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
2.13
6JGE[A] β-D-Glcp-(1-2)-β-D-Glcp-(1-1)-methyl
1.92
6JGG[A] β-D-Glcp-(1-2)-β-D-Glcp-(1-1)-methyl
1.90
6JGK[A] β-D-6-deoxy-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp1SH
1.66
6JGL[A] 1.92
6JGN[A] β-D-Glcp-(1-3)-β-D-Glcp3S
1.98
6JGO[A] β-D-Glcp-(1-4)-β-D-Glcp4S
1.95
6JGP[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.99
6JGQ[A] β-D-Glcp-(1-2)-β-D-Glcp-(1-1)-methyl
2.01
6JGR[A] β-D-Glcp-(1-3)-β-D-Glcp3S
2.25
6JGS[A] β-D-Glcp-(1-4)-β-D-Glcp4S
2.03
6JGT[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.82
6K6V[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.98
6KUF[A] β-D-Glcp
1.90
6L1J[A] β-D-Glcp-(1-3)-β-D-Glcp3S
1.80
6LBB[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp1SH
1.80
6LBV[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
1.89
6LC5[A] β-D-Glcp-(1-3)-β-D-Glcp3S
1.76
6MD6[A] β-D-Glcp-(1-2)-β-D-Glcp-(1-1)-methyl
1.68
6MI1[A] β-D-Glcp-(1-6)-β-D-Glcp6SH-(1-1)-methyl
2.30
 β-glucosidase I (Bgl;KmBglI) 3.2.1.21 Kluyveromyces marxianus NBRC1777 ACY95404.1 D1GCC6
3ABZ[A,B,C,D] 2.15
3AC0[A,B,C,D] β-D-Glcp
2.54
 β-xylosidase / β-glucosidase (Lxyl-p1-2) (7-β-xylosyltaxane-active) 3.2.1.21
3.2.1.37
Lentinula edodes M95.33 AET31459.1
AET31460.1
G8GLP2
6JBS[A,B,C,D] 2.40
6KJ0[A,B] β-D-Xylp
2.27
7EY1[A]
7EY2[A]
 β-glucosidase (Bgl6;Gh3-3;NcGH3-3;NcCel3A;Ncrα-Bgl101;NCU08755) (Cel3A) 3.2.1.21 Neurospora crassa OR74A EAA26868.1
XP_330872.1
XP_956104.1
Q7RWP2
5NBS[A,B] 2.25
 xylan 1,4-β-xylosidase (PcBxl3) 3.2.1.37 Phanerochaete chrysosporium    
7VC6[A] 2.54
7VC7[A] β-D-Xylp
3.08
 β-glucosidase (ReCel3A) (Cel3A) 3.2.1.21 Rasamsonia emersonii IMI 392299 AAL69548.3
AAL69548.2
Q8TGI8
4D0J[A,B,C,D] β-D-Glcp
2.20
5JU6[A,B,C,D] β-D-Glcp
2.20
 β-N-acetylglucosaminidase (Nag;RmNag) 3.2.1.52 Rhizomucor miehei CAU432 AGC24356.1 V9M3A9
4ZM6[A,B] 2.80
 β-glucosidase (tomatinase;B2Tom) 3.2.1.- Septoria lycopersici AAB08445.1
AAB08446.1
Q99324
cryst    
 β-glucosidase (Bgl) 3.2.1.21 Thermochaetoides thermophila CT2 ABR57325.2
ABI63598.1
ABR57325.1
A6YRT4
Q09IZ3
6SZ6[A] D-1-deoxy-Manp
α-D-Manp-(1-2)-α-D-Manp-(1-6)-D-1-deoxy-Manp
β-D-Glcp
β-D-Manp-(1-3)-D-1-deoxy-GlcpNAc
2.99
 β-xylosidase (Bxl1; TrXyl3A) (Xyl3A) 3.2.1.37 Trichoderma reesei CAA93248.1
CAW52645.1
CBC23920.1
Q92458
5A7M[A,B] 1.80
5AE6[A,B] β-D-Xylp-(1-4)-α-D-Xylp
2.10
 β-glucosidase 1 (Bgl1;Bgl-1;TrBGL1;HjCel3A) (Cel3A) 3.2.1.21 Trichoderma reesei QM9414 AAA18473.1 Q12715
3ZYZ[A] β-D-Glcp
2.10
3ZZ1[A] 2.10
4I8D[A,B] β-D-Glcp
2.48
unclassified
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 β-glucosidase (JMB19063) 3.2.1.21 compost metagenome    
3U48[B] β-D-Glcp
2.20
3U4A[A,B] β-D-Glcp
β-D-Glcp-(1-4)-β-D-Glcp
2.20
3U48[A] β-D-Glcp
2.20
 broad-specificity β-glucosidase/β-xylosidase (GlyA1) (partial) 3.2.1.21
3.2.1.37
metagenome    
5K6M[A] β-D-Glcp
2.17
5K6N[A] β-D-Xylp
2.08
5K6O[A] β-D-Galp
2.29
5K6L[A] 1.83
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