Glycoside Hydrolase Family 13

Known Activitiesα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); amino acid transporter
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesAAMY; CAZypedia; EBI Protein of the Month; HOMSTRAD; InterPro; PDB Molecule of the Month; PFAM; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (14130); Uniprot accession (7548); PDB accession (334); 3D entries (80); cryst (5)
All (11200) Archaea (169) Bacteria (9234) Eukaryota (1676) Viruses (2) unclassified (119) Structure (80 - 5 cryst) Characterized (695) Subfamilies (8873)
Archaea
Protein Name EC#OrganismGenBankUniprotPDB/3D Subf
 TreY (fragment)   Acidianus ambivalens DSM 3772 CBY66041.1     26
 Ahos_1028   Acidianus hospitalis W1 AEE93914.1     11
 Ahos_1027   Acidianus hospitalis W1 AEE93913.1     26
 Ahos_1029   Acidianus hospitalis W1 AEE93915.1     10
 ASAC_1074   Acidilobus saccharovorans 345-15 ADL19479.1 D9Q2E1  
 Arcve_0957   Archaeoglobus veneficus SNP6 AEA46968.1    
 DKAM_0273   Desulfurococcus kamchatkensis 1221n ACL10599.1 B8D2L1  
 Desmu_0336   Desulfurococcus mucosus DSM 2162 ADV64655.1    
 pullulanase (ApuA;Desmu_0653) 3.2.1.41 Desulfurococcus mucosus DSM 2162 AAG31033.1
ADV64961.1
Q9HHB0  
 HacjB3_03370   Halalkalicoccus jeotgali B3 ADJ14067.1 D8J7H2  
 α-amylase (AmyH) 3.2.1.1 Haloarcula hispanica CAI64586.1 Q4A3E0  
 HAH_0971 (Agla1)   Haloarcula hispanica ATCC 33960 CGMCC 1.2049 AEM56589.1     31
 HAH_2473 (MalA)   Haloarcula hispanica ATCC 33960 CGMCC 1.2049 AEM58060.1    
 HAH_2792 (Amy2)   Haloarcula hispanica ATCC 33960 CGMCC 1.2049 AEM58372.1    
 HAH_1360 (fragment)   Haloarcula hispanica ATCC 33960 CGMCC 1.2049 AEM56974.1    
 rrnAC2350 (Amy)   Haloarcula marismortui ATCC 43049 AAV47170.1    
 rrnAC0224 (AglA1)   Haloarcula marismortui ATCC 43049 AAV45283.1
CBF79161.1
    31
 rrnAC1962 (MalA)   Haloarcula marismortui ATCC 43049 AAV46830.1    
 α-cyclomaltodextrin glucanotransferase (Cgt) 2.4.1.19 Haloferax mediterranei CAI46245.1 Q53I75   2
 HVO_0567   Haloferax volcanii DS2 ADE03517.1 D4GSG2  
 HVO_1710   Haloferax volcanii DS2 ADE04704.1 D4H055  
 Hbor_26440   Halogeometricum borinquense DSM 11551 ADQ68196.1 E4NT99  
 Hmuk_0209   Halomicrobium mukohataei DSM 12286 ACV46346.1 C7NWY1   20
 Hmuk_2260   Halomicrobium mukohataei DSM 12286 ACV48372.1 C7NXD8   31
 Hmuk_0052   Halomicrobium mukohataei DSM 12286 ACV46203.1 C7NVT8   31
 Hmuk_0051   Halomicrobium mukohataei DSM 12286 ACV46202.1 C7NVT7   31
 Hmuk_0210   Halomicrobium mukohataei DSM 12286 ACV46347.1 C7NWY2   2
 Halxa_4124   Halopiger xanaduensis SH-6 AEH38729.1     20
 Hqrw_2193 (Amya1)   Haloquadratum walsbyi C23 CCC40085.1    
 Hqrw_2840 (Amya2)   Haloquadratum walsbyi C23 CCC40652.1    
 HQ2031A   Haloquadratum walsbyi strain: DSM 16790 = HBSQ001 CAJ52158.1 Q18IL2  
 HQ2540A   Haloquadratum walsbyi strain: DSM 16790 = HBSQ001 CAJ52652.1 Q18H91  
 Huta_1052   Halorhabdus utahensis DSM 12940 ACV11235.1 C7NVF1  
 Hlac_0544   Halorubrum lacusprofundi ATCC 49239 ACM56146.1 B9LTL2  
 Hlac_0587 (fragment)   Halorubrum lacusprofundi ATCC 49239 ACM56189.1 B9LTQ5   16
 Hlac_1504   Halorubrum lacusprofundi ATCC 49239 ACM57092.1 B9LP04  
 Htur_2652   Haloterrigena turkmenica DSM 5511 ADB61528.1 D2RWM7  
 Htur_2110 (possible fragment)   Haloterrigena turkmenica DSM 5511 ADB60993.1 D2RTP2  
 Mcup_1211   Metallosphaera cuprina Ar-4 AEB95316.1     11
 Mcup_1210   Metallosphaera cuprina Ar-4 AEB95315.1     10
 Mcup_1212   Metallosphaera cuprina Ar-4 AEB95317.1     26
 maltooligosyltrehalose trehalohydrolase (TreZ;MTH) 3.2.1.141 Metallosphaera hakonensis JCM 8857 AAR18372.1 Q6SZP6   10
 maltooligosyltrehalose synthase (TreY;MTS) 5.4.99.15 Metallosphaera hakonensis JCM 8857 AAR18371.1 Q6SZP7   26
 glycogen debranching enzyme (TreX)   Metallosphaera hakonensis JCM 8857 AAS00512.1 Q6R5P1   11
 Memar_0386   Methanoculleus marisnigri JR1 ABN56319.1 A3CSG9  
 Memar_1265   Methanoculleus marisnigri JR1 ABN57195.1 A3CUZ5   9
 MCON_0285   Methanosaeta concilii GP-6 AEB67166.1    
 Mhar_1650   Methanosaeta harundinacea 6Ac AET65010.1    
 Mzhil_0346   Methanosalsum zhilinae DSM 4017 AEH60222.1     5
 MA1641   Methanosarcina acetivorans C2A AAM05050.1
NP_616570.1
Q8TQA8  
 MA_2000 (TreX)   Methanosarcina acetivorans C2A AAM05403.1
NP_616923.1
Q8TPB3   11
 MA3032   Methanosarcina acetivorans C2A AAM06405.1
NP_617925.1
Q8TLK4  
 Mbar_A1790   Methanosarcina barkeri str. Fusaro AAZ70731.1 Q46BL1  
 Mbar_A2517   Methanosarcina barkeri str. Fusaro AAZ71430.1 Q469L2   11
 Nmag_1154   Natrialba magadii ATCC 43099 ADD04738.1 D3SRN3  
 maltotriose-forming α-amylase 3.2.1.1
3.2.1.116
Natronococcus sp. Ah-36 BAA05516.1 Q60224  
 PTO0068   Picrophilus torridus DSM 9790 AAT42653.1 Q6L2Z8   3
 PTO0067   Picrophilus torridus DSM 9790 AAT42652.1 Q6L2Z9   9
 trehalose synthase (PTO0069) 5.4.99.16 Picrophilus torridus DSM 9790 AAT42654.1
CBF79071.1
Q6L2Z7   16
 PAE0318   Pyrobaculum aerophilum str. IM2 AAL62712.1
NP_558530.1
Q8ZZC9  
 Pars_2149   Pyrobaculum arsenaticum DSM 13514 ABP51695.1 A4WMS8  
 Pcal_0222   Pyrobaculum calidifontis JCM 11548 ABO07659.1 A3MSP2  
 Pisl_1967   Pyrobaculum islandicum DSM 4184 ABL89114.1 A1RVY2  
 P186_1084   Pyrobaculum sp. 1860 AET32520.1    
 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13) 3.2.1.1
3.2.1.54
Pyrococcus furiosus DSM 3638 AAL82063.1
CAZ78721.1
NP_579668.1
Q8TZP8  
 β-cyclodextrin glucanotransferase (PfCgt;PF0478;PF0478*) 2.4.1.19 Pyrococcus furiosus DSM 3638 AAL80602.1
ABA30024.1
ABA30025.1
ABA33720.1
NP_578207.1
Q3HUR2
Q8U3I8
  2
 α-amylase (AmyA;PF0477) 3.2.1.1 Pyrococcus furiosus DSM 3638 AAB67705.1
AAC45663.1
AAL80601.1
NP_578206.1
O08452
Q8U3I9
  7
 α-amylase (AmyA) 3.2.1.1 Pyrococcus woesei AAD54338.1
AAF44693.1
ACH26211.1
Q7LYT7
Q9P9L0
1MWO[A]
1MXD[A]
1MXG[A]
7
 PYCH_18550   Pyrococcus yayanosii CH1 AEH25510.1     7
 PYCH_18540   Pyrococcus yayanosii CH1 AEH25509.1     2
 PYCH_17070   Pyrococcus yayanosii CH1 AEH25366.1     20
 Shell_0233   Staphylothermus hellenicus DSM 12710 ADI31372.1 D7DB25  
 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) 3.2.1.133
3.2.1.54
Staphylothermus marinus F1 ABN69720.1 A3DM60 2XIE
4AEE[A,B]
 α-amylase/maltooligosyltrehalose trehalohydrolase (TreZ) 3.2.1.1
3.2.1.141
Sulfolobus acidocaldarius BAA11011.1
BAA11863.1
Q53641   10
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Sulfolobus acidocaldarius BAA11009.1
BAA11865.1
Q53688 1IV8[A] 26
 isoamylase (TreX) 3.2.1.68 Sulfolobus acidocaldarius ATCC33909 BAA11864.1 O05152   11
 Saci_1439   Sulfolobus acidocaldarius DSM 639 AAY80765.1     11
 Saci_1440 (TreZ)   Sulfolobus acidocaldarius DSM 639 AAY80766.1     10
 TreX (fragment)   Sulfolobus islandicus B9B AAP93396.1 Q6X9I6  
 TreX (fragment)   Sulfolobus islandicus D4 AAP93395.1 Q6X9I7  
 SiH_0855   Sulfolobus islandicus HVE10/4 ADX82208.1     26
 SiH_0856   Sulfolobus islandicus HVE10/4 ADX82209.1     11
 SiH_0857   Sulfolobus islandicus HVE10/4 ADX82210.1     10
 TreX (fragment)   Sulfolobus islandicus I7 AAP93398.1 Q6X9I9  
 TreX (fragment)   Sulfolobus islandicus K10B AAP93394.1 Q6X9I9  
 LD85_0438   Sulfolobus islandicus L.D.8.5 ADB86216.1 D2PG22   10
 LD85_0437   Sulfolobus islandicus L.D.8.5 ADB86215.1 D2PG21   11
 LD85_0436   Sulfolobus islandicus L.D.8.5 ADB86214.1 D2PG20   26
 LS215_0444   Sulfolobus islandicus L.S.2.15 ACP34570.1 C3MLH2   11
 LS215_0445   Sulfolobus islandicus L.S.2.15 ACP34571.1 C3MLH3   10
 LS215_0443   Sulfolobus islandicus L.S.2.15 ACP34569.1 C3MLH1   26
 M1425_0478   Sulfolobus islandicus M.14.25 ACP37333.1 C3MUX0   11
 M1425_0477   Sulfolobus islandicus M.14.25 ACP37332.1 C3MUW9   10
 M1425_0479   Sulfolobus islandicus M.14.25 ACP37334.1 C3MUX1   26
 M1627_0491   Sulfolobus islandicus M.16.27 ACP54489.1 C3N2K1   11
 M1627_0490   Sulfolobus islandicus M.16.27 ACP54488.1 C3N2K0   10
 M1627_0492   Sulfolobus islandicus M.16.27 ACP54490.1 C3N2K2   26
 M164_0513   Sulfolobus islandicus M.16.4 ACR41144.1 C4KE21   11
 M164_0514   Sulfolobus islandicus M.16.4 ACR41145.1 C4KE22   26
 M164_0512   Sulfolobus islandicus M.16.4 ACR41143.1 C4KE20   10
 TreX (fragment)   Sulfolobus islandicus N15B AAP93399.1 Q6X9I9  
 TreX (fragment)   Sulfolobus islandicus N23A AAP93391.1 Q6X9I9  
 TreX (fragment)   Sulfolobus islandicus N38D AAP93400.1 Q6X9I2  
 TreX (fragment)   Sulfolobus islandicus REN1H1 AAP93397.1 Q6X9I5  
 SiRe_0490   Sulfolobus islandicus REY15A ADX84576.1     26
 SiRe_0486   Sulfolobus islandicus REY15A ADX84572.1     10
 SiRe_0487+SiRe_0489   Sulfolobus islandicus REY15A ADX84575.1     11
 TreX (fragment)   Sulfolobus islandicus U3C AAP93392.1 Q6X9J0  
 TreX (fragment)   Sulfolobus islandicus V8 AAP93393.1 Q6X9I9  
 YG5714_0403   Sulfolobus islandicus Y.G.57.14 ACP44695.1 C3NA11   11
 YG5714_0404   Sulfolobus islandicus Y.G.57.14 ACP44696.1 C3NA12   10
 YG5714_0402   Sulfolobus islandicus Y.G.57.14 ACP44694.1 C3NA10   26
 YN1551_2617   Sulfolobus islandicus Y.N.15.51 ACP49544.1 C3NLJ1   11
 YN1551_2616   Sulfolobus islandicus Y.N.15.51 ACP49543.1 C3NLJ0   10
 YN1551_2618   Sulfolobus islandicus Y.N.15.51 ACP49545.1 C3NLJ2   26
 maltooligosyltrehalose synthase (TreY) 5.4.99.15 Sulfolobus shibatae AAF17554.1
AAM81591.1
Q9UWN8   26
 maltooligosyl trehalose trehalohydrolase (TreZ) 3.2.1.141 Sulfolobus shibatae AAF17553.1
AAM81589.1
Q9UWN9   10
 glycogen debranching enzyme (TreX) 2.4.1.25
3.2.1.68
Sulfolobus shibatae B12 AAM81590.1 Q8NKQ3   11
 Ssol_2807   Sulfolobus solfataricus 98/2 ACX92889.1 D0KPY5   26
 Ssol_2806   Sulfolobus solfataricus 98/2 ACX92888.1 D0KPY4   11
 Ssol_2805   Sulfolobus solfataricus 98/2 ACX92887.1 D0KPY3   10
 α-amylase / maltooligosyltrehalose trehalohydrolase 3.2.1.1
3.2.1.141
Sulfolobus solfataricus KM1 AAR46454.1
BAA11010.1
Q55088 1EH9[A]
1EHA[A]
10
 glycosyltrehalose-producing enzyme 5.4.99.15 Sulfolobus solfataricus KM1 BAA11008.1 Q55262   26
 maltooligosyltrehalose synthase (TreY;SSO2095) 5.4.99.15 Sulfolobus solfataricus P2 AAK42274.1
CAA69505.1
NP_343484.1
P95869   26
 maltooligosyltrehalose trehalohydrolase (TreZ;SSO2093;c06020) 3.2.1.141 Sulfolobus solfataricus P2 AAK42272.1
CAA69503.1
CAC23737.1
NP_343482.1
P95867   10
 isoamylase / 4-α-glucanotransferase (TreX;SSO2094;GlgX;ORF-c06_004;c06021) 2.4.1.25
3.2.1.68
Sulfolobus solfataricus P2 AAK42273.1
CAA69504.1
CAC23738.1
NP_343483.1
P95868 2VNC[A,B]
2VR5[A,B]
2VUY[A,B]
11
 ST0926 + ST0927   Sulfolobus tokodaii str. 7 BAB65938.1
BAB65939.1
NP_376829.1
NP_376830.1
Q973H4
Q973H5
 
 ST0928   Sulfolobus tokodaii str. 7 BAB65940.1
NP_376831.1
Q973H3   11
 ST0929   Sulfolobus tokodaii str. 7 BAB65941.1
NP_376832.1
Q973H2 3HJE[A] 26
 pullulanase type III (PulhA) 3.2.1.41 Thermococcus aggregans CAB94218.1
CAZ78888.1
Q9P9A0  
 TERMP_01455   Thermococcus barophilus MP ADT84430.1     2
 TERMP_01653   Thermococcus barophilus MP ADT84628.1     20
 TGAM_0222 (AmyA-1)   Thermococcus gammatolerans EJ3 EJ3; DSM 15229 ACS32724.1 C5A3B2   7
 TGAM_0599   Thermococcus gammatolerans EJ3 EJ3; DSM 15229 ACS33101.1 C5A4D9  
 TGAM_1752   Thermococcus gammatolerans EJ3 EJ3; DSM 15229 ACS34254.1 C5A1I5  
 α-amylase (Amy) 3.2.1.1 Thermococcus hydrothermalis AL662 AAC97877.1
AAC97878.1
ACH26213.1
O93647
O93648
  7
 β-cyclodextrin glucanotransferase (Cgt;TK-cgt;TK2172) 2.4.1.19 Thermococcus kodakarensis KOD1 BAB78538.1
BAD86361.1
CAT70763.1
Q8X268   2
 TK1770   Thermococcus kodakarensis KOD1 BAD85959.1
CAT70564.1
Q5JJ59  
 TK0977   Thermococcus kodakarensis KOD1 BAD85166.1
CAT69621.1
CAZ78793.1
Q5JID9  
 α-amylase (ApkA;TK1884) 3.2.1.1 Thermococcus kodakarensis KOD1 ACH26210.1
BAA21130.1
BAD86073.1
CAT70635.1
O33476
Q5JER7
  7
 TON_1451   Thermococcus onnurineus NA1 ACJ16941.1 B6YXX8   2
 TON_1796   Thermococcus onnurineus NA1 ACJ17286.1 B6YV58  
 α-amylase (TO_Amyl;TON_1660) 3.2.1.1 Thermococcus onnurineus NA1 ABA26948.1
ACJ17150.1
B6YUG5   7
 α-amylase (BD5031) 3.2.1.1 Thermococcus sp. 'AEPII 1a' AAM48113.1
ACH26099.1
Q8NKR4   7
 GQS_07090   Thermococcus sp. 4557 AEK73316.1     7
 GQS_08270   Thermococcus sp. 4557 AEK73548.1     2
 GQS_06205 (fragment)   Thermococcus sp. 4557 AEK73139.1     20
 TAM4_475   Thermococcus sp. AM4 EEB74530.1    
 TAM4_1203   Thermococcus sp. AM4 EEB73455.2     7
 TAM4_1607   Thermococcus sp. AM4 EEB74240.2    
 α-cyclodextrin glucanotransferase (CgtA) 2.4.1.19 Thermococcus sp. B1001 BAA88217.1
BAB18101.1
CAZ78889.1
Q9UWN2   2
 cyclomaltodextrinase (CdnA) 3.2.1.133
3.2.1.54
Thermococcus sp. B1001 BAB18100.1
CAZ78887.1
Q9HHC8   20
 α-amylase (BD5064) 3.2.1.1 Thermococcus sp. GU5L5 AAM48112.1
ACH26098.1
Q8NKR5   7
 α-amylase (THJA) 3.2.1.1 Thermococcus sp. HJ21 ABU98335.1 B4X9V8   7
 α-amylase 3.2.1.1 Thermococcus sp. Rt3 AAB87860.1
ACH26212.1
O50200   7
 α-amylase (Amy) 3.2.1.1 Thermococcus thioreducens AAT11125.1 Q66SB4   7
 Tpen_1454   Thermofilum pendens Hrk 5 ABL78851.1 A1S071   2
 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458) 3.2.1.133
3.2.1.54
Thermofilum pendens Hrk 5 ABL78855.1 A1S075  
 Ta0138   Thermoplasma acidophilum DSM 1728 CAC11285.1
CAZ78711.1
NP_393616.1
Q9HLT7   20
 maltogenic α-amylase (TpMA;TVG0226058) 3.2.1.133 Thermoplasma volcanium GSS1 BAB59359.1
CAZ78693.1
NP_110736.1
Q97C86   20
 TTX_1744   Thermoproteus tenax Kra 1 CCC82365.1    
 TUZN_1763   Thermoproteus uzoniensis 768-20 AEA13224.1    
 Tagg_0445   Thermosphaera aggregans DSM 11486 ADG90720.1 D5U0S0  
 Tagg_0897   Thermosphaera aggregans DSM 11486 ADG91169.1 D5U219  
 BSM_26470 (fragment)   uncultured archaeon CBH39169.1 D1JAN9  
 Amy (fragment)   uncultured archaeon CCF72376.1    
 ORF (fragment)   uncultured haloarchaeon ABQ76050.1 A5YSU3  
 Vdis_2000   Vulcanisaeta distributa DSM 14429 ADN51372.1 E1QP07  
 VMUT_0412   Vulcanisaeta moutnovskia 768-28 ADY00624.1    




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