Glycoside Hydrolase Family 13 / Subf 12

Activities in Sub Family	[retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1119); Uniprot accession (10); PDB accession (5); 3D entries (2); cryst (0)

Summary

Download GH13_12 (1094) Taxonomic display Structure (2) Characterized (7)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
PXH68_08985		Streptococcus sp. 29896	WNY46997.1			12
GP482_05035		Streptococcus sp. CNU 77-61	QGZ27537.1			12
P8P68_08410		Streptococcus sp. D7B5	WFR87108.1			12
BZG42_09390		Streptococcus sp. DAT741	QHF55740.1			12
RSK81_02390		Streptococcus sp. DTU_2020_1000888_1_SI_GRL_NUU_041A	WNU95257.1			12
RRU92_04015		Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006	WNS73202.1			12
A6J31_04115		Streptococcus sp. FDAARGOS_192	ARC22447.1			12
EGX96_00460		Streptococcus sp. FDAARGOS_520	AYZ03791.1			12
EGX72_05755		Streptococcus sp. FDAARGOS_521	AYY68504.1			12
EGX70_01410		Streptococcus sp. FDAARGOS_522	AYY63607.1			12
A6J68_07785		Streptococcus sp. group B FDAARGOS_229	ARC25065.1			12
N597_07175		Streptococcus sp. I-P16	AGY38741.1			12
FPL13_01080		Streptococcus sp. KS 6	QOG24249.1			12
NCTC10228_00299 (PulA)		Streptococcus sp. NCTC 10228	VUC95740.1			12
NCTC11567_00265 (Pula_1)		Streptococcus sp. NCTC 11567	VUC95940.1			12
SNAG_1661 (PulA)		Streptococcus sp. NPS 308	BAV80530.1			12
AXF18_04620		Streptococcus sp. oral taxon 064 W10853	ANR75233.1			12
SP4011_19350 (PulA)		Streptococcus sp. SP4011	BDT65518.1			12
V470_08675		Streptococcus sp. VT 162	AHZ48483.1			12
J5M87_05775		Streptococcus sp. zg-86	QTH47077.1			12
HAS68_18330		Streptococcus suis 05HAS68	AHF60382.1			12
SSU05_2064		Streptococcus suis 05ZYH33	ABP91030.1			12
J1N58_10920		Streptococcus suis 1112S	QTA56773.1			12
NLY77_10930		Streptococcus suis 1521251	UTI50781.1			12
NOV99_01235		Streptococcus suis 2018WUSS151	UUD28974.1			12
KPA27_09970		Streptococcus suis 39565	QWS31069.1			12
AN924_12530		Streptococcus suis 90-1330	AMU79709.1			12
SSU98_2067		Streptococcus suis 98HAH33	ABP93225.1			12
SSUA7_1881		Streptococcus suis A7	AER45198.1			12
CWI26_11230		Streptococcus suis AH681	AUA20210.1			12
K6969_11495		Streptococcus suis AKJ18	QZT29262.1			12
SSUBM407_1919		Streptococcus suis BM407	CAZ56775.1			12
CR541_10155		Streptococcus suis CS100322	AUW25021.1			12
CVO91_11965		Streptococcus suis CZ130302	ATZ04784.1			12
SSUD12_2036		Streptococcus suis D12	AER20299.1			12
SSUD9_2082		Streptococcus suis D9	AER18240.1			12
DAT299_18930		Streptococcus suis DAT299	BCK44329.1			12
DAT300_20650		Streptococcus suis DAT300	BCK46526.1			12
K6974_12255		Streptococcus suis FJSM5	QZT17323.1			12
JZ789_11230		Streptococcus suis GX69	UCS84739.1			12
SSGZ1_1875		Streptococcus suis GZ1	ADE32331.1			12
CR542_10835		Streptococcus suis HA0609	AUW27125.1			12
DF184_10260		Streptococcus suis HN105	AWL27081.1			12
CWM22_12525		Streptococcus suis HN136	AUC92906.1			12
JSY00_09725		Streptococcus suis ID32098	UAJ07511.1			12
JM964_09715		Streptococcus suis ID33329	QRA08557.1			12
KWY62_09705		Streptococcus suis ID41570	QXT27337.1			12
FPT06_05895		Streptococcus suis INT-01	QDS24732.1			12
DK235_09870		Streptococcus suis ISU1606	QLL46305.1			12
DK877_10750		Streptococcus suis ISU2414	QLL54526.1			12
DK875_01045		Streptococcus suis ISU2514	QLL50425.1			12
DK237_10580		Streptococcus suis ISU2614	QLL48369.1			12
DK878_10445		Streptococcus suis ISU2660	QLL56572.1			12
DK876_09775		Streptococcus suis ISU2714	QLL52441.1			12
SSUJS14_2019		Streptococcus suis JS14	ADV71067.1			12
DP112_10390		Streptococcus suis JSHA1003	AXI68628.1			12
L3X06_09535		Streptococcus suis LSM157	UJX22995.1			12
GRI05_10035		Streptococcus suis LSM178	QOJ58189.1			12
L3X15_09950		Streptococcus suis LSM29	UJX21085.1			12
NLY76_09760		Streptococcus suis LSS42	UTI55090.1			12
NCTC10234_01832 (PulA)		Streptococcus suis NCTC10234	SQH01447.1			12
NCTC10237_02011 (Amyx_1)		Streptococcus suis NCTC10237	VTT09768.1			12
K6972_09975		Streptococcus suis NJ3	QZS60861.1			12
bifunctional amylopullulanase (ApuA;SSU1849)	3.2.1.41	Streptococcus suis P1/7	CAR47543.1	C5VVZ9		12
YYK_08920		Streptococcus suis S735	AFR01291.1			12
NJAUSS_1903		Streptococcus suis SC070731	AGG65362.1			12
JZY07_10810		Streptococcus suis SC183	QSQ90889.1			12
B9H01_09920		Streptococcus suis SC19	ARL71004.1			12
SSUSC84_1871		Streptococcus suis SC84	CAZ52647.1			12
D2A30_00670		Streptococcus suis SFJ44	ULL21961.1			12
DP111_10590		Streptococcus suis SH1510	AXI66567.1			12
SSU12_1998		Streptococcus suis SS12	AER16173.1			12
BVD85_09755		Streptococcus suis SS2-1	AUW23015.1			12
K6976_10320		Streptococcus suis SS389	QZS51181.1			12
SSUST1_1962		Streptococcus suis ST1	AER22304.1			12
SSUST3_1908		Streptococcus suis ST3	AEB82319.1			12
NLY35_10645		Streptococcus suis STC104	UTH71026.1			12
NKZ79_10640		Streptococcus suis STC78	UTH54218.1			12
NKZ64_10645		Streptococcus suis STC80	UTH56320.1			12
NKZ66_10630		Streptococcus suis STC81	UTH58423.1			12
NKZ67_10645		Streptococcus suis STC83	UTH60525.1			12
NKZ65_10640		Streptococcus suis STC84	UTH62630.1			12
NKZ80_10630		Streptococcus suis STC85	UTH64723.1			12
NKZ81_10630		Streptococcus suis STC86	UTH66826.1			12
NKZ63_10645		Streptococcus suis STC90	UTH68928.1			12
K8227_09820		Streptococcus suis SZ1908	UAM75953.1			12
NLY75_10695		Streptococcus suis T15	UTI57160.1			12
T15_2129		Streptococcus suis T15	AGZ24199.1			12
TL13_1866		Streptococcus suis TL13	AGL48789.1			12
K6967_09505		Streptococcus suis Transconjugant cAKJ18	QZT31398.1			12
K6971_09505		Streptococcus suis Transconjugant cDY107	QZT25231.1			12
K6970_09480		Streptococcus suis Transconjugant cFJSM5	QZT21466.1			12
K6968_09520		Streptococcus suis Transconjugant cNJ3	QZT19568.1			12
K6975_09425		Streptococcus suis Transconjugant cSS389	QZT23331.1			12
NOV96_02100		Streptococcus suis WUSS030	UYZ66668.1			12
E8M06_10465		Streptococcus suis WUSS351	QCE39873.1			12
YB51_9480		Streptococcus suis YB51	AGW88268.1			12
I5W22_09905		Streptococcus suis YP20190405	QPO28661.1			12
I5V48_11190		Streptococcus suis YZDH1	QPO26486.1			12
STHERMO_1064 (fragment)		Streptococcus thermophilus	CAD0176855.1			12
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