Activities in Sub Family	[retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1119); Uniprot accession (10); PDB accession (5); 3D entries (2); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
NCTC13737_01597 (PulA)		Streptococcus pyogenes NCTC13737	SQH23678.1			12
NCTC13738_01683 (PulA)		Streptococcus pyogenes NCTC13738	SQG21471.1			12
NCTC13739_01575 (PulA)		Streptococcus pyogenes NCTC13739	SQH09492.1			12
NCTC13742_01730 (PulA)		Streptococcus pyogenes NCTC13742	SQG25213.1			12
NCTC13743_01658 (PulA)		Streptococcus pyogenes NCTC13743	SQG23556.1			12
NCTC13744_01648 (PulA)		Streptococcus pyogenes NCTC13744	SQG73831.1			12
NCTC13745_01682 (PulA)		Streptococcus pyogenes NCTC13745	SQH48715.1			12
NCTC13751_01775 (PulA)		Streptococcus pyogenes NCTC13751	SQH63346.1			12
NCTC4001_01601 (PulA)		Streptococcus pyogenes NCTC4001	SQF51119.1			12
NCTC5163_01804 (PulA)		Streptococcus pyogenes NCTC5163	SQE57837.1			12
NCTC5164_01804 (PulA)		Streptococcus pyogenes NCTC5164	SQE91330.1			12
NCTC8193_00228 (PulA)		Streptococcus pyogenes NCTC8193	VTP70472.1			12
NCTC8195_01797 (PulA)		Streptococcus pyogenes NCTC8195	SQF46900.1			12
ERS445054_01807 (PulA)		Streptococcus pyogenes NCTC8198	CKH14036.1			12
NCTC8224_01637 (PulA)		Streptococcus pyogenes NCTC8224	SRX91589.1			12
NCTC8225_01778 (PulA)		Streptococcus pyogenes NCTC8225	SQE39341.1			12
NCTC8227_01669 (PulA)		Streptococcus pyogenes NCTC8227	SQG53752.1			12
NCTC8230_01811 (PulA)		Streptococcus pyogenes NCTC8230	SQF61696.1			12
NCTC8231_01577 (PulA)		Streptococcus pyogenes NCTC8231	SQF33352.1			12
NCTC8232_01822 (PulA)		Streptococcus pyogenes NCTC8232	VEE00684.1			12
NCTC8300_01604 (PulA)		Streptococcus pyogenes NCTC8300	SQF02682.1			12
NCTC8302_01123 (PulA)		Streptococcus pyogenes NCTC8302	VEF03332.1			12
NCTC8304_01628 (PulA)		Streptococcus pyogenes NCTC8304	SQG97462.1			12
NCTC8314_01704 (PulA)		Streptococcus pyogenes NCTC8314	SQE94409.1			12
NCTC8316_01580 (PulA)		Streptococcus pyogenes NCTC8316	SRX89884.1			12
NCTC8318_01655 (PulA)		Streptococcus pyogenes NCTC8318	VTR16001.1			12
NCTC8320_01759 (PulA)		Streptococcus pyogenes NCTC8320	SQG44981.1			12
NCTC8322_01719 (PulA)		Streptococcus pyogenes NCTC8322	SRX88247.1			12
NCTC8324_01778 (PulA)		Streptococcus pyogenes NCTC8324	SQF39828.1			12
NCTC8326_01742 (PulA)		Streptococcus pyogenes NCTC8326	SQF58201.1			12
NCTC8328_01684 (PulA)		Streptococcus pyogenes NCTC8328	SQF11428.1			12
NCTC8332_01804 (PulA)		Streptococcus pyogenes NCTC8332	SQF18554.1			12
NCTC8370_01634 (PulA)		Streptococcus pyogenes NCTC8370	SQH68903.1			12
SD89_08805		Streptococcus pyogenes NGAS322	AKZ51180.1			12
DK93_07625		Streptococcus pyogenes NGAS327	AKI39303.1			12
DK92_07940		Streptococcus pyogenes NGAS596	AKI37729.1			12
SD90_07870		Streptococcus pyogenes NGAS638	AKZ52825.1			12
DI45_09060		Streptococcus pyogenes NGAS743	AKI36123.1			12
C5P47_08030		Streptococcus pyogenes NGAS979	AWS23856.1			12
A4265_07400		Streptococcus pyogenes NS53	ANC75067.1			12
Spy49_1626c (PulA)	3.2.1.41	Streptococcus pyogenes NZ131	ACI61883.1 CAD32942.1	A0A0H3C2B1 B5XIM1 Q8KLP1		12
EHF39_08795		Streptococcus pyogenes RLGH	AZA34211.1			12
SP119_1661		Streptococcus pyogenes S119	VDC39969.1			12
D1F63_08795		Streptococcus pyogenes SASM4-Duke	AYJ03114.1			12
D0Z58_08695		Streptococcus pyogenes SP1336	QBC35630.1			12
SPs1696		Streptococcus pyogenes SSI-1	BAC64791.1 NP_802958.1			12
VT08_08040		Streptococcus pyogenes STAB09014	ANP30136.1			12
B4W66_08505		Streptococcus pyogenes STAB090229	ASQ21902.1			12
EZY40_07760		Streptococcus pyogenes STAB09023	QGU91239.1			12
VU19_08525		Streptococcus pyogenes STAB10015	AKL63847.1			12
EZ612_07760		Streptococcus pyogenes STAB10048	QGU92847.1			12
STAB1102_08180		Streptococcus pyogenes STAB1102	AIG49513.1			12
B5D85_08855		Streptococcus pyogenes STAB120304	ASQ23714.1			12
AXK13_08250		Streptococcus pyogenes STAB13021	AND05521.1			12
AYM92_08595		Streptococcus pyogenes STAB14018	ARV01933.1			12
STAB901_08180		Streptococcus pyogenes STAB901	AIG50879.1			12
STAB902_09135		Streptococcus pyogenes STAB902	AIG47963.1			12
SpyM51651		Streptococcus pyogenes str. Manfredo	CAM30972.1			12
D8S76_08615		Streptococcus pyogenes TSPY155	QAX76867.1			12
EB817_08215		Streptococcus pyogenes TSPY165	QAX71761.1			12
EB818_08195		Streptococcus pyogenes TSPY208	QAX73430.1			12
EB816_09140		Streptococcus pyogenes TSPY453	QAX70055.1			12
D8S77_08290		Streptococcus pyogenes TSPY556	QAX75113.1			12
GUT183_18500		Streptococcus ruminantium GUT-183	BDD39612.1			12
GUT184_17740		Streptococcus ruminantium GUT-184	BDD41510.1			12
GUT189_18070		Streptococcus ruminantium GUT-189	BDD43474.1			12
SR187_9520		Streptococcus ruminantium GUT187T	BBA93505.1			12
GPZ88_00935		Streptococcus ruminicola CNU G2	QIM45704.1			12
P7Y79_02960		Streptococcus ruminicola CNU_77-47	WFM82273.1			12
Ssal_01131 (AmyA)		Streptococcus salivarius 57.I	AEJ53430.1			12
V471_07820		Streptococcus salivarius ATCC 25975	ARI60136.1			12
NX99_01915		Streptococcus salivarius ATCC 27945	ARI57271.1			12
SALIVB_1059 (PulA)		Streptococcus salivarius CCHSS3	CCB93348.1			12
HRE60_04755		Streptococcus salivarius DB-B5	QMI50994.1			12
A6J87_10485		Streptococcus salivarius FDAARGOS_259	ARC49756.1			12
HSISS4_00917		Streptococcus salivarius HSISS4	ALR79929.1			12
BSR14_04990		Streptococcus salivarius ICDC1	QGU78608.1			12
BSR19_05425		Streptococcus salivarius ICDC2	QGU80592.1			12
BSR20_04990		Streptococcus salivarius ICDC3	QGU82629.1			12
AWB63_04975		Streptococcus salivarius JF	AMB82773.1			12
SALIVA_1072 (PulA)		Streptococcus salivarius JIM8777	CCB95387.1			12
FHI56_07130		Streptococcus salivarius LAB813	QEM32671.1			12
RDV59_06405		Streptococcus salivarius LMG 11489	WMS34550.1			12
SSAL8618_05340		Streptococcus salivarius NCTC 8618	AIY21166.1			12
NCTC7366_01030 (AmyA)		Streptococcus salivarius NCTC7366	SQF75951.1			12
NCTC8618_03506 (AmyA)		Streptococcus salivarius NCTC8618	VED88765.1			12
LV497_00165		Streptococcus salivarius SALI-10	UOT90656.1			12
FFV08_09630		Streptococcus sanguinis CGMH010	QLB52829.1			12
FDP16_09475		Streptococcus sanguinis CGMH058	QLB50688.1			12
FOC72_07990		Streptococcus sanguinis FDAARGOS_770	QKQ44493.1			12
NCTC10904_01861 (Pula_2)		Streptococcus sanguinis NCTC 10904	VDY72576.1			12
NCTC11085_00468 (Pula_2)		Streptococcus sanguinis NCTC11085	SQF33985.1			12
NCTC11086_01914 (Pula_2)		Streptococcus sanguinis NCTC11086	SQF71961.1			12
NCTC3168_01155 (Pula_1)		Streptococcus sanguinis NCTC3168	VEE19260.1			12
NCTC7863_00439 (Pula_2)		Streptococcus sanguinis NCTC7863	SQG29635.1			12
SSA_0453		Streptococcus sanguinis SK36	ABN43901.1			12
ORF (fragment)		Streptococcus sp.	CAM56050.1			12
UKS_16570		Streptococcus sp. 116-D4	BBP10455.1			12
FD735_08540		Streptococcus sp. 1643	QCZ58382.1			12
PW252_10525		Streptococcus sp. 29887	WNY50986.1			12
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