Glycoside Hydrolase Family 13 / Subf 12

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (473); Uniprot accession (51); PDB accession (5); 3D entries (2); cryst (0)
| 1 | 2 | 3 | 4 | 5 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 SanJ4211_1620c   Streptococcus anginosus J4211 ALL03707.1     12
 ANG_0493   Streptococcus anginosus subsp. whileyi MAS624 BAN60963.1     12
 NCTC11371_01695 (Pula_2)   Streptococcus australis NCTC13166 SQH66992.1     12
 SCI_1667 (Pula2)   Streptococcus constellatus subsp. pharyngis C1050 AGU80565.1     12
 SCRE_1623 (Pula2)   Streptococcus constellatus subsp. pharyngis C232 AGU73420.1     12
 SCR2_1623 (Pula2)   Streptococcus constellatus subsp. pharyngis C818 AGU75174.1     12
 I872_08425   Streptococcus cristatus AS 1.3089 AGK71765.1     12
 NCTC13807_01740 (PulA)   Streptococcus cristatus NCTC13807 SQI48732.1     12
 FGCSD_0294 (PulA)   Streptococcus dysgalactiae Kdys0611 BBE39520.1     12
 SDSE167_0246   Streptococcus dysgalactiae subsp. equisimilis 167 BAN92651.1     12
 SDSE_0243 (PulA)   Streptococcus dysgalactiae subsp. equisimilis AC-2713 CCI61754.1     12
 SDE12394_01035   Streptococcus dysgalactiae subsp. equisimilis ATCC 12394 ADX23755.1     12
 SDEG_0237   Streptococcus dysgalactiae subsp. equisimilis GGS_124 BAH80754.1 C5WED9   12
 NCTC5370_00246 (Pula_1)   Streptococcus dysgalactiae subsp. equisimilis NCTC5370 SQE85055.1     12
 NCTC5371_00257 (Pula_1)   Streptococcus dysgalactiae subsp. equisimilis NCTC5371 SQF77185.1     12
 NCTC6179_00298 (Pula_1)   Streptococcus dysgalactiae subsp. equisimilis NCTC6179 SQF66153.1     12
 NCTC7136_00269 (Pula_1)   Streptococcus dysgalactiae subsp. equisimilis NCTC7136 SQG92109.1     12
 NCTC9414_00257 (Pula_1)   Streptococcus dysgalactiae subsp. equisimilis NCTC9414 SQF68269.1     12
 GGS_0220 (PulA)   Streptococcus dysgalactiae subsp. equisimilis RE378 BAM60393.1     12
 SEQ_0944   Streptococcus equi subsp. equi 4047 CAW93478.1 C0M7W1   12
 SE071780_00975   Streptococcus equi subsp. equi ATCC 39506 ASB96578.1     12
 SeseC_01097 (GlgX)   Streptococcus equi subsp. zooepidemicus ATCC 35246 AEJ25113.1     12
 Q426_05065   Streptococcus equi subsp. zooepidemicus CY AIA67739.1     12
 Sez_0820 (PulA)   Streptococcus equi subsp. zooepidemicus MGCS10565 ACG62179.1 B4U2G2   12
 NCTC11606_00846 (PulA)   Streptococcus equi subsp. zooepidemicus NCTC11606 SQF53871.1     12
 NCTC11824_00831 (PulA)   Streptococcus equi subsp. zooepidemicus NCTC11824 SQG17029.1     12
 NCTC12090_00853 (PulA)   Streptococcus equi subsp. zooepidemicus NCTC12090 SQF05373.1     12
 NCTC6176_00885 (PulA)   Streptococcus equi subsp. zooepidemicus NCTC6176 SQF81519.1     12
 NCTC7022_00824 (PulA)   Streptococcus equi subsp. zooepidemicus NCTC7022 SQE95706.1     12
 A6J79_08025   Streptococcus equinus FDAARGOS_251 ARC34343.1     12
 NCTC12278_01219 (PulA)   Streptococcus ferus NCTC12278 SQF40647.1     12
 AU077_02895 (fragment)   Streptococcus gallolyticus ICDDRB-NRC-S1 ALT81803.1     12
 NCTC13773_01743 (PulA)   Streptococcus gallolyticus NCTC13773 SQG79920.1     12
 SGGB_1458   Streptococcus gallolyticus subsp. gallolyticus ATCC 43143 BAK28316.1     12
 SGGBAA2069_c14850 (PulA1)   Streptococcus gallolyticus subsp. gallolyticus ATCC BAA-2069 CBZ48657.1     12
 BTR42_08205   Streptococcus gallolyticus subsp. gallolyticus DSM 16831 AQP42613.1     12
 Sbs3   Streptococcus gallolyticus subsp. gallolyticus TX20005 ACX81318.1 D0UFB3   12
 GALLO_1462   Streptococcus gallolyticus UCN34 CBI13953.1 D3HEJ0   12
 AU079_05880 (fragment)   Streptococcus infantarius ICDDRB-NRC-S5 ALT82840.1     12
 Sinf_1270   Streptococcus infantarius subsp. infantarius CJ18 AEZ62583.1     12
 CTW00_00888 (PulA)   Streptococcus iniae FP5228 ATX39088.1     12
 DQ08_01405   Streptococcus iniae ISET0901 AHY15164.1     12
 DW64_01400   Streptococcus iniae ISNO AHY17035.1     12
 QMA0248_0303   Streptococcus iniae QMA0248 ASL34144.1     12
 K710_0292   Streptococcus iniae SF1 AGM98096.1     12
 SIR_1606 (Pula2)   Streptococcus intermedius B196 AGU76955.1     12
 SII_1592 (Pula2)   Streptococcus intermedius C270 AGU78754.1     12
 A6J72_07020   Streptococcus intermedius FDAARGOS_233 ARC26950.1     12
 SCIM_1403   Streptococcus intermedius JTH08 BAM24056.1     12
 RN88_07535   Streptococcus intermedius KCOM 1545 ALF28341.1     12
 NCTC11324_01586 (Pula2)   Streptococcus intermedius NCTC11324 SQH52535.1     12
 SITYG_17130   Streptococcus intermedius TYG1620 BAW17690.1     12
 NCTC13774_01239 (AmyX) (fragment)   Streptococcus lutetiensis NCTC13774 SQG57263.1     12
 NCTC8738_01471 (AmyX) (fragment)   Streptococcus lutetiensis NCTC8738 SQF42650.1     12
 SMA_1464 (PulA)   Streptococcus macedonicus ACA-DC 198 CCF02755.1     12
 SAMEA4412692_00309 (Pula2)   Streptococcus merionis NCTC13788 SNU86624.1     12
 smi_1848   Streptococcus mitis B6 CBJ23088.1 D3HB38   12
 RN80_03180   Streptococcus mitis KCOM 1350 (= ChDC B183) ALD67483.1     12
 AXK38_09010   Streptococcus mitis SVGS_061 AMH89373.1     12
 CO686_08010   Streptococcus oralis FDAARGOS_367 ATF57379.1     12
 BWR56_0240   Streptococcus oralis S.MIT/ORALIS-351 AQA08523.1     12
 SOR_1714   Streptococcus oralis Uo5 CBZ01365.1     12
 SGPB_1362   Streptococcus pasteurianus ATCC 43144 BAK30402.1     12
 NCTC13784_01571 (PulA)   Streptococcus pasteurianus NCTC13784 SQI08991.1     12
 Sequence 4083 from patent US 7074914 (partial)   Streptococcus pneumoniae ABI04044.1
ABI09085.1
ABJ32601.1
ABJ50214.1
ABL17957.1
ACC15069.1
ACC20625.1
    12
 BUM80_10520   Streptococcus pneumoniae 11A AUC46706.1     12
 CWI64_08325   Streptococcus pneumoniae 19F AUB33599.1     12
 pullulanase (SpuA) 3.2.1.41 Streptococcus pneumoniae 3.B AAG33958.1 Q9F930   12
 C4N11_01360   Streptococcus pneumoniae 335 AVD74288.1     12
 SP670_0342   Streptococcus pneumoniae 670-6B ADM90417.1 E0TQZ4   12
 SP70585_0328   Streptococcus pneumoniae 70585 ACO17918.1 C1CB43   12
 T308_01310   Streptococcus pneumoniae A026 AGZ47087.1     12
 A66_00266   Streptococcus pneumoniae A66 CRI60977.1     12
 SPAP_0317   Streptococcus pneumoniae AP200 ADM83910.1 E0SVD1   12
 SPN23F02560   Streptococcus pneumoniae ATCC 700669 CAR68116.1 B8ZKT7   12
 SPCG_0278   Streptococcus pneumoniae CGSP14 ACB89530.1 B2ISJ3   12
 DA395_01690   Streptococcus pneumoniae CP2215 AVV90680.1     12
 SPND122_00278   Streptococcus pneumoniae D122 AOG55229.1     12
 SPND141_00281   Streptococcus pneumoniae D141 AOG57311.1     12
 SPND219_00298   Streptococcus pneumoniae D219 ANO36183.1     12
 SPD_0250   Streptococcus pneumoniae D39 ABJ53695.1 Q04MI0   12
 SPV_0250 (SpuA)   Streptococcus pneumoniae D39V AVN85423.1     12
 SPG_0253 (PulA)   Streptococcus pneumoniae G54 ACF55912.1 B5E6U2   12
 HMPREF1038_00325 (SpuA)   Streptococcus pneumoniae gamPNI0373 AFS42401.1     12
 SPNHU15_00346   Streptococcus pneumoniae Hu15 ARD36159.1     12
 SPNHU17_00342   Streptococcus pneumoniae Hu17 ARD33958.1     12
 SPH_0386   Streptococcus pneumoniae Hungary19A-6 ACA37282.1 B1I8Z6   12
 INV104_02270   Streptococcus pneumoniae INV104 CBW35911.1 E1XPB5   12
 SPNINV200_02500   Streptococcus pneumoniae INV200 CBW33884.1 E1XHU3   12
 SPJ_0278   Streptococcus pneumoniae JJA ACO18730.1 C1CC59   12
 KK0381_19670 (PulA)   Streptococcus pneumoniae KK0381 BAX32087.1     12
 KK0981_23030 (PulA)   Streptococcus pneumoniae KK0981 BAW83799.1     12
 KK1157_20820 (PulA)   Streptococcus pneumoniae KK1157 BAX34379.1     12
 MDRSPN_00251 (PulA)   Streptococcus pneumoniae MDRSPN001 BBA58457.1     12
 NCTC11902_00289 (Pula_1)   Streptococcus pneumoniae NCTC11902 SQG97928.1     12
 NCTC13276_00256 (Pula_1)   Streptococcus pneumoniae NCTC13276 SQG33538.1     12
 ERS445053_00223 (Pula_1)   Streptococcus pneumoniae NCTC7465 COT01062.1     12
 NCTC7466_00257 (Pula_1)   Streptococcus pneumoniae NCTC7466 SQG01316.1     12
 SpnNT_00275 (PulA)   Streptococcus pneumoniae NT_110_58 AJD71216.1     12
 SPNOXC_02880   Streptococcus pneumoniae OXC141 CBW31948.1 E1XEJ2   12
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