Activities in Sub Family	[retaining] pullulanase (EC 3.2.1.41); Isoamylase (EC 3.2.1.68);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1119); Uniprot accession (10); PDB accession (5); 3D entries (2); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
STHERMO_1212 (fragment)		Streptococcus thermophilus isolate STH_CIRM_368	CAD0145340.1			12
STHERMO_1076 (fragment)		Streptococcus thermophilus isolate STH_CIRM_65	CAD0137684.1			12
STHERMO_1073 (fragment)		Streptococcus thermophilus isolate STH_CIRM_65	CAD0137678.1			12
STHERMO_1074 (fragment)		Streptococcus thermophilus isolate STH_CIRM_65	CAD0137680.1			12
STHERMO_1256 (fragment)		Streptococcus thermophilus isolate STH_CIRM_67	CAD0192972.1			12
STHERMO_1255 (fragment)		Streptococcus thermophilus isolate STH_CIRM_67	CAD0192971.1			12
STHERMO_1253 (fragment)		Streptococcus thermophilus isolate STH_CIRM_67	CAD0192969.1			12
STHERMO_1066 (fragment)		Streptococcus thermophilus isolate STH_CIRM_772	CAD0142048.1			12
STHERMO_1068 (fragment)		Streptococcus thermophilus isolate STH_CIRM_772	CAD0142054.1			12
STHERMO_1067 (fragment)		Streptococcus thermophilus isolate STH_CIRM_772	CAD0142051.1			12
STHERMO_1110 (fragment)		Streptococcus thermophilus isolate STH_CIRM_956	CAD0141823.1			12
STHERMO_1108 (fragment)		Streptococcus thermophilus isolate STH_CIRM_956	CAD0141819.1			12
STHERMO_1109 (fragment)		Streptococcus thermophilus isolate STH_CIRM_956	CAD0141821.1			12
STHERMO_1107 (fragment)		Streptococcus thermophilus isolate STH_CIRM_956	CAD0141817.1			12
STHERMO_1128 (fragment)		Streptococcus thermophilus isolate STH_CIRM_961	CAD0147672.1			12
STHERMO_1130 (fragment)		Streptococcus thermophilus isolate STH_CIRM_961	CAD0147674.1			12
STHERMO_1129 (fragment)		Streptococcus thermophilus isolate STH_CIRM_961	CAD0147673.1			12
STHERMO_1127 (fragment)		Streptococcus thermophilus isolate STH_CIRM_961	CAD0147671.1			12
STHERMO_1158 (fragment)		Streptococcus thermophilus isolate STH_CIRM_967	CAD0150060.1			12
STHERMO_1156 (fragment)		Streptococcus thermophilus isolate STH_CIRM_967	CAD0150058.1			12
STHERMO_1157 (fragment)		Streptococcus thermophilus isolate STH_CIRM_967	CAD0150059.1			12
STHERMO_1131 (fragment)		Streptococcus thermophilus isolate STH_CIRM_998	CAD0152412.1			12
STHERMO_1128 (fragment)		Streptococcus thermophilus isolate STH_CIRM_998	CAD0152409.1			12
STHERMO_1130 (fragment)		Streptococcus thermophilus isolate STH_CIRM_998	CAD0152411.1			12
STHERMO_1127 (fragment)		Streptococcus thermophilus isolate STH_CIRM_998	CAD0152408.1			12
STHERMO_1129 (fragment)		Streptococcus thermophilus isolate STH_CIRM_998	CAD0152410.1			12
STH8232_1226 (fragment)		Streptococcus thermophilus JIM 8232	CCC19920.1			12
STH8232_1227 (fragment)		Streptococcus thermophilus JIM 8232	CCC19921.1			12
STH8232_1228 (fragment)		Streptococcus thermophilus JIM 8232	CCC19922.1			12
BEN15_08255 (fragment)		Streptococcus thermophilus KLDS 3.1003	AOD27394.1			12
BEN15_08270 (fragment)		Streptococcus thermophilus KLDS 3.1003	AOD27037.1			12
BEN15_08265 (fragment)		Streptococcus thermophilus KLDS 3.1003	AOD27036.1			12
A9497_01325 (fragment)		Streptococcus thermophilus KLDS SM	ANJ62050.1			12
A9497_01320 (fragment)		Streptococcus thermophilus KLDS SM	ANJ62049.1			12
A9497_01315 (fragment)		Streptococcus thermophilus KLDS SM	ANJ63505.1			12
A9497_01330 (fragment)		Streptococcus thermophilus KLDS SM	ANJ62051.1			12
stu1033 (fragment)		Streptococcus thermophilus LMG 18311	AAV60690.1			12
stu1031 (fragment)		Streptococcus thermophilus LMG 18311	AAV60688.1			12
AMD33_03480 (fragment)		Streptococcus thermophilus MN-BM-A01	ALD16897.1			12
AMD33_03470 (fragment)		Streptococcus thermophilus MN-BM-A01	ALD16895.1			12
AMD33_03475 (fragment)		Streptococcus thermophilus MN-BM-A01	ALD16896.1			12
MNA02_1005 (fragment)		Streptococcus thermophilus MN-BM-A02	AKH33458.1			12
MNA02_1004 (fragment)		Streptococcus thermophilus MN-BM-A02	AKH33457.1			12
Y1U_C0875 (fragment)		Streptococcus thermophilus MN-ZLW-002	AFJ83324.1			12
StMoz109_04785 (fragment)		Streptococcus thermophilus Moz109	QYK22188.1			12
StMoz109_04780 (fragment)		Streptococcus thermophilus Moz109	QYK22187.1			12
StMoz109_04775 (fragment)		Streptococcus thermophilus Moz109	QYK22231.1			12
StMoz109_04795 (fragment)		Streptococcus thermophilus Moz109	QYK22274.1			12
StMoz109_04790 (fragment)		Streptococcus thermophilus Moz109	QYK22189.1			12
STN4L_00532 (fragment)		Streptococcus thermophilus N4L	SSC62488.1			12
STN4L_00530 (fragment)		Streptococcus thermophilus N4L	SSC62486.1			12
STN4L_00535 (fragment)		Streptococcus thermophilus N4L	SSC62491.1			12
STN4L_00531 (fragment)		Streptococcus thermophilus N4L	SSC62487.1			12
STN4L_00529 (fragment)		Streptococcus thermophilus N4L	SSC62485.1			12
NCTC12958_01261 (Pula_1) (fragment)		Streptococcus thermophilus NCTC12958	SQF25065.1			12
NCTC12958_01262 (Pula_2) (fragment)		Streptococcus thermophilus NCTC12958	SQF25066.1			12
NCTC12958_01263 (AmyX) (fragment)		Streptococcus thermophilus NCTC12958	SQF25067.1			12
STND_0981 (fragment)		Streptococcus thermophilus ND03	ADQ63033.1			12
BBD27_1796 (fragment)		Streptococcus thermophilus ND07	AOZ59880.1			12
BBD27_1797 (fragment)		Streptococcus thermophilus ND07	AOZ59881.1			12
AVT04_00040 (fragment)		Streptococcus thermophilus S9	ALX90366.1			12
AVT04_00045 (fragment)		Streptococcus thermophilus S9	ALX90367.1			12
SMQ301_1027 (fragment)		Streptococcus thermophilus SMQ-301	AKB97647.1			12
SMQ301_1026 (fragment)		Streptococcus thermophilus SMQ-301	AKB97646.1			12
SMQ301_1028 (fragment)		Streptococcus thermophilus SMQ-301	AKB97648.1			12
D1O36_05170 (fragment)		Streptococcus thermophilus ST106	AXT15468.1			12
D1O36_05165 (fragment)		Streptococcus thermophilus ST106	AXT15467.1			12
D1O36_05155 (fragment)		Streptococcus thermophilus ST106	AXT16269.1			12
DV947_04940 (fragment)		Streptococcus thermophilus ST109	AXN98183.1			12
BGL51_05230 (fragment)		Streptococcus thermophilus ST3	ASX19380.1			12
BGL51_05225 (fragment)		Streptococcus thermophilus ST3	ASX19379.1			12
BGL51_05215 (fragment)		Streptococcus thermophilus ST3	ASX19377.1			12
BGL51_05220 (fragment)		Streptococcus thermophilus ST3	ASX19378.1			12
BGL51_05235 (fragment)		Streptococcus thermophilus ST3	ASX19381.1			12
ST64987_0913 (fragment)		Streptococcus thermophilus ST64987	QIS47430.1			12
ST64987_0914 (fragment)		Streptococcus thermophilus ST64987	QIS47431.1			12
GFB63_09810 (fragment)		Streptococcus thermophilus TK-P3A	QOH31644.1			12
STYK_17520		Streptococcus toyakuensis TP1632	BDB09938.1			12
NCTC4674_00296 (PulA)		Streptococcus uberis NCTC4674	SQG82435.1			12
NCTC3166_01593 (Pula_2)		Streptococcus viridans NCTC3166	VED67760.1			12
CL176_10630		Suicoccus acidiformans ZY16052	AXY26410.1			12
B4U37_19775		Sutcliffiella horikoshii 20a	ART78141.1			12
K7887_19965		Sutcliffiella horikoshii BAT	UAL49675.1			12
O1A01_18975		Sutcliffiella sp. NC1	WBL13975.1			12
ODU73_001889 (fragment)		Thermoclostridium stercorarium RKWS1	UZQ84825.1			12
ODU73_001888 (fragment)		Thermoclostridium stercorarium RKWS1	UZQ84824.1			12
CSTERLE_09010		Thermoclostridium stercorarium subsp. leptospartum DSM 9219	ANX01702.1			12
Cst_c18540		Thermoclostridium stercorarium subsp. stercorarium DSM 8532	AGI39830.1 AGC68831.1			12
CSTERTH_08925		Thermoclostridium stercorarium subsp. thermolacticum DSM 2910	ANW99138.1			12
EW093_04485		Thiospirochaeta perfilievii	QEN03988.1			12
amylopullulanase (Ss4;PulSS4)	3.2.1.41	uncultured bacterium	AGL50935.1	R4NSY4		12
pullulanase (fragment)		uncultured bacterium	AIE44109.1			12
KBP50_13655		Virgibacillus pantothenticus DSM 26	QTY14959.1			12
BKP57_16300		Virgibacillus sp. 6R	API93235.1			12
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