Glycoside Hydrolase Family 13 / Subf 12

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (338); Uniprot accession (51); PDB accession (5); 3D entries (2); cryst (0)
| 1 | 2 | 3 | 4 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 AXY_17360   Amphibacillus xylanus NBRC 15112 BAM47868.1     12
 AXY_17370   Amphibacillus xylanus NBRC 15112 BAM47869.1     12
 BBEV_0319 (Pulα-1)   Bacillus beveridgei MLTeJB AOM81713.1     12
 BBEV_0399 (Pulα-2)   Bacillus beveridgei MLTeJB AOM81793.1     12
 BH3697   Bacillus halodurans C-125 BAB07416.1
NP_244564.1
Q9K6N1   12
 B4U37_19775   Bacillus horikoshii 20a ART78141.1     12
 N288_22515   Bacillus infantis NRRL B-14911 AGX06343.1     12
 BpOF4_04230   Bacillus pseudofirmus OF4 ADC48912.1 D3FXS5   12
 Bsel_0993   [Bacillus] selenitireducens MLS10 ADH98514.1 D6Y0C3   12
 alkaline amylase-pullulanase 3.2.1.1
3.2.1.41
Bacillus sp. KSM-1378 AAS36537.1
AAS36539.1
BAA11332.1
P70983   12
 pullulanase (Pul1876)   Bacillus sp. KSM-1876 BAB47586.1 Q93UZ9   12
 CSTERLE_09010   [Clostridium] stercorarium subsp. leptospartum DSM 9219 ANX01702.1     12
 Cst_c18540   [Clostridium] stercorarium subsp. stercorarium DSM 8532 AGI39830.1
AGC68831.1
    12
 CSTERTH_08925   [Clostridium] stercorarium subsp. thermolacticum DSM 2910 ANW99138.1     12
 HBHAL_2962 (PulA)   Halobacillus halophilus DSM 2266 CCG45310.1     12
 CEH05_09750   Halobacillus halophilus HL2HP6 ASF39393.1     12
 HM131_10095   Halobacillus mangrovi KTB 131 ARI79178.1     12
 BW721_00145   Jeotgalibaca sp. PTS2502 APZ48227.1     12
 Nther_0868   Natranaerobius thermophilus JW/NM-WN-LF ACB84453.1 B2A887   12
 SAMN05444162_3517   Paenibacillaceae bacterium GAS479 SDT23904.1     12
 PBOR_10995   Paenibacillus borealis DSM 13188 AIQ57390.1     12
 A3958_11090   Paenibacillus glucanolyticus 5162 ANA80481.1     12
 PGRAT_10190   Paenibacillus graminis DSM 15220 AIQ67951.1     12
 BBD41_12600   Paenibacillus ihbetae IHBB 9852 ANY73354.1     12
 PRIO_2159   Paenibacillus riograndensis SBR5 CQR54568.1     12
 H70357_10230   Paenibacillus sp. FSL H7-0357 AIQ16995.1     12
 H70737_08620   Paenibacillus sp. FSL H7-0737 AIQ22910.1     12
 P40081_10735   Paenibacillus sp. FSL P4-0081 AIQ28595.1     12
 R50345_09135   Paenibacillus sp. FSL R5-0345 AIQ34761.1     12
 R50912_10265   Paenibacillus sp. FSL R5-0912 AIQ40360.1     12
 R70723_09840   Paenibacillus sp. FSL R7-0273 AIQ46154.1     12
 R70331_09720   Paenibacillus sp. FSL R7-0331 AIQ51763.1     12
 GYMC10_0346   Paenibacillus sp. Y412MC10 ACX62652.1 D3E9K1   12
 SAMN05661091_5635   Paenibacillus uliginis N3/975 SMF91957.1     12
 BBI08_08755   Planococcus halocryophilus DSM 24743 ANU15501.1     12
 Plano_2303   Planococcus sp. PAMC 21323 AIY06268.1     12
 SZO_11410   Steptococcus equi subsp. zooepidemicus H70 CAW99566.1     12
 SA111_01387   Streptococcus agalactiae CZT39498.1     12
 BQ8897_BM110_01307   Streptococcus agalactiae SIW57750.1     12
 BSA_12940   Streptococcus agalactiae 09mas018883 CCW38063.1     12
 pullulanase type I (Sap;BibA;SAG1216;SAG_1216) 3.2.1.41 Streptococcus agalactiae 2603V/R AAN00098.1
NP_688225.1
    12
 CHF17_01305   Streptococcus agalactiae 874391 ASZ01560.1     12
 SAK_1302   Streptococcus agalactiae A909 ABA44447.1     12
 GT95_06810   Streptococcus agalactiae C001 ASI66315.1     12
 W903_1299   Streptococcus agalactiae CNCTC 10/84 AIX04977.1     12
 pullulanase type I (SAP;GBSCOH1_1122)   Streptococcus agalactiae COH1 CDN66588.1
EAO76676.1
Q3DB05 3FAW[A]
3FAX[A]
12
 BB161_06985   Streptococcus agalactiae CUGBS591 ASA79917.1     12
 CUGBS08_01248 (PulA)   Streptococcus agalactiae CU_GBS_08 AMQ14799.1     12
 CUGBS98_01185 (PulA)   Streptococcus agalactiae CU_GBS_98 AMQ16791.1     12
 A6J82_06735   Streptococcus agalactiae FDAARGOS_254 ARC45052.1     12
 A9256_06275   Streptococcus agalactiae FWL1402 ANR97252.1     12
 AOY37_06500   Streptococcus agalactiae GBS ST-1 ALP87760.1     12
 AV644_06280   Streptococcus agalactiae GBS-M002 APS25249.1     12
 DK41_06455   Streptococcus agalactiae GBS1-NY AIK71770.1     12
 DK42_06750   Streptococcus agalactiae GBS2-NM AIK73888.1     12
 DX05_06775   Streptococcus agalactiae GBS6 AIK75937.1     12
 GBS85147_1194   Streptococcus agalactiae GBS85147 AKI57619.1     12
 A964_1188   Streptococcus agalactiae GD201008-001 AFS45953.1     12
 GX064_06125   Streptococcus agalactiae GX064 AKU00170.1     12
 SAH002_06250   Streptococcus agalactiae H002 AKU03734.1     12
 SAHN016_06210   Streptococcus agalactiae HN016 AKT96296.1     12
 MSA_13420   Streptococcus agalactiae ILRI005 CCW40200.1     12
 SAIL_13330   Streptococcus agalactiae ILRI112 CCW42347.1     12
 Gbs1288   Streptococcus agalactiae NEM316 CAD46947.1
NP_735732.1
    12
 EN72_06720   Streptococcus agalactiae NGBS061 AIF86771.1     12
 AMM49_06330   Streptococcus agalactiae NGBS128 AMD32522.1     12
 AMR84_06765   Streptococcus agalactiae NGBS357 AOF51262.1     12
 EN73_06255   Streptococcus agalactiae NGBS572 AIF88788.1     12
 B1H24_06955   Streptococcus agalactiae Sag37 AQY24504.1     12
 AMD29_06515   Streptococcus agalactiae SG-M1 ALB16227.1     12
 BB194_06615   Streptococcus agalactiae SG-M158 ASA84071.1     12
 BB197_06615   Streptococcus agalactiae SG-M163 ASA82018.1     12
 BB164_07225   Streptococcus agalactiae SG-M25 ASA90319.1     12
 BB268_06615   Streptococcus agalactiae SG-M29 ASA88175.1     12
 BB165_06240   Streptococcus agalactiae SG-M4 ASA99129.1     12
 BB313_06610   Streptococcus agalactiae SG-M50 ASA86121.1     12
 BB162_06260   Streptococcus agalactiae SG-M6 ASA94434.1     12
 BB159_06570   Streptococcus agalactiae SG-M8 ASA92440.1     12
 RDF_1212   Streptococcus agalactiae SS1 AKI95627.1     12
 BBP08_02675   Streptococcus agalactiae WC1535 AOQ20213.1     12
 YM001_06150   Streptococcus agalactiae YM001 AKT98242.1     12
 SAIN_1534 (Pula2)   Streptococcus anginosus C1051 AGU82249.1     12
 SANR_1773 (Pula2)   Streptococcus anginosus C238 AGU84182.1     12
 SanJ4211_1620c   Streptococcus anginosus J4211 ALL03707.1     12
 ANG_0493   Streptococcus anginosus subsp. whileyi MAS624 BAN60963.1     12
 SCI_1667 (Pula2)   Streptococcus constellatus subsp. pharyngis C1050 AGU80565.1     12
 SCRE_1623 (Pula2)   Streptococcus constellatus subsp. pharyngis C232 AGU73420.1     12
 SCR2_1623 (Pula2)   Streptococcus constellatus subsp. pharyngis C818 AGU75174.1     12
 I872_08425   Streptococcus cristatus AS 1.3089 AGK71765.1     12
 SDSE167_0246   Streptococcus dysgalactiae subsp. equisimilis 167 BAN92651.1     12
 SDSE_0243 (PulA)   Streptococcus dysgalactiae subsp. equisimilis AC-2713 CCI61754.1     12
 SDE12394_01035   Streptococcus dysgalactiae subsp. equisimilis ATCC 12394 ADX23755.1     12
 SDEG_0237   Streptococcus dysgalactiae subsp. equisimilis GGS_124 BAH80754.1 C5WED9   12
 GGS_0220 (PulA)   Streptococcus dysgalactiae subsp. equisimilis RE378 BAM60393.1     12
 SEQ_0944   Streptococcus equi subsp. equi 4047 CAW93478.1 C0M7W1   12
 SE071780_00975   Streptococcus equi subsp. equi ATCC 39506 ASB96578.1     12
 SeseC_01097 (GlgX)   Streptococcus equi subsp. zooepidemicus ATCC 35246 AEJ25113.1     12
 Q426_05065   Streptococcus equi subsp. zooepidemicus CY AIA67739.1     12
 Sez_0820 (PulA)   Streptococcus equi subsp. zooepidemicus MGCS10565 ACG62179.1 B4U2G2   12
 A6J79_08025   Streptococcus equinus FDAARGOS_251 ARC34343.1     12
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