Glycoside Hydrolase Family 13 / Subf 12

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (341); Uniprot accession (51); PDB accession (5); 3D entries (2); cryst (0)
| 1 | 2 | 3 | 4 |
Bacteria
Protein Name EC#Organism GenBankUniprotPDB/3DSubf
 MGAS1882_1586 (PulA)   Streptococcus pyogenes MGAS1882 AFC68767.1     12
 MGAS2096_Spy1703 (PulA)   Streptococcus pyogenes MGAS2096 ABF36754.1 Q1J9Q7   12
 MGAS23530_1500 (PulA)   Streptococcus pyogenes MGAS23530 ANC26153.1     12
 MGAS27061_1535 (PulA)   Streptococcus pyogenes MGAS27061 ANC27781.1     12
 PulA   Streptococcus pyogenes MGAS315 AAM80301.1
NP_665498.1
Q8K5S2   12
 M5005_Spy1680 (PulA)   Streptococcus pyogenes MGAS5005 AAZ52298.2     12
 M28_Spy1668 (PulA)   Streptococcus pyogenes MGAS6180 AAX72778.1     12
 SpyM18_2038   Streptococcus pyogenes MGAS8232 AAL98513.1
NP_608014.1
Q8NZB0   12
 MGAS9429_Spy1681 (PulA)   Streptococcus pyogenes MGAS9429 ABF32868.1 Q1JJV5   12
 MTB313_1656 (PulA)   Streptococcus pyogenes MTB313 BAQ52044.1     12
 MTB314_1559 (PulA)   Streptococcus pyogenes MTB314 BAQ53706.1     12
 ERS445054_01807 (PulA)   Streptococcus pyogenes NCTC8198 CKH14036.1     12
 SD89_08805   Streptococcus pyogenes NGAS322 AKZ51180.1     12
 DK93_07625   Streptococcus pyogenes NGAS327 AKI39303.1     12
 DK92_07940   Streptococcus pyogenes NGAS596 AKI37729.1     12
 SD90_07870   Streptococcus pyogenes NGAS638 AKZ52825.1     12
 DI45_09060   Streptococcus pyogenes NGAS743 AKI36123.1     12
 A4265_07400   Streptococcus pyogenes NS53 ANC75067.1     12
 Spy49_1626c (PulA) 3.2.1.41 Streptococcus pyogenes NZ131 ACI61883.1
CAD32942.1
B5XIM1
Q8KLP1
  12
 SPs1696   Streptococcus pyogenes SSI-1 BAC64791.1
NP_802958.1
F7IVX1   12
 VT08_08040   Streptococcus pyogenes STAB09014 ANP30136.1     12
 B4W66_08505   Streptococcus pyogenes STAB090229 ASQ21902.1     12
 VU19_08525   Streptococcus pyogenes STAB10015 AKL63847.1     12
 STAB1102_08180   Streptococcus pyogenes STAB1102 AIG49513.1     12
 B5D85_08855   Streptococcus pyogenes STAB120304 ASQ23714.1     12
 AXK13_08250   Streptococcus pyogenes STAB13021 AND05521.1     12
 AYM92_08595   Streptococcus pyogenes STAB14018 ARV01933.1     12
 STAB901_08180   Streptococcus pyogenes STAB901 AIG50879.1     12
 STAB902_09135   Streptococcus pyogenes STAB902 AIG47963.1     12
 SpyM51651   Streptococcus pyogenes str. Manfredo CAM30972.1 A2RGJ0   12
 Ssal_01131 (AmyA)   Streptococcus salivarius 57.I AEJ53430.1     12
 V471_07820   Streptococcus salivarius ATCC 25975 ARI60136.1     12
 NX99_01915   Streptococcus salivarius ATCC 27945 ARI57271.1     12
 SALIVB_1059 (PulA)   Streptococcus salivarius CCHSS3 CCB93348.1     12
 A6J87_10485   Streptococcus salivarius FDAARGOS_259 ARC49756.1     12
 HSISS4_00917   Streptococcus salivarius HSISS4 ALR79929.1     12
 AWB63_04975   Streptococcus salivarius JF AMB82773.1     12
 SALIVA_1072 (PulA)   Streptococcus salivarius JIM8777 CCB95387.1     12
 SSAL8618_05340   Streptococcus salivarius NCTC 8618 AIY21166.1     12
 SSA_0453   Streptococcus sanguinis SK36 ABN43901.1 A3CL46   12
 ORF (fragment)   Streptococcus sp. CAM56050.1     12
 A6J31_04115   Streptococcus sp. FDAARGOS_192 ARC22447.1     12
 A6J68_07785   Streptococcus sp. group B FDAARGOS_229 ARC25065.1     12
 N596_05315   Streptococcus sp. I-G2 AGY40221.1     12
 N597_07175   Streptococcus sp. I-P16 AGY38741.1     12
 SNAG_1661 (PulA)   Streptococcus sp. NPS 308 BAV80530.1     12
 AXF18_04620   Streptococcus sp. oral taxon 064 W10853 ANR75233.1     12
 V470_08675   Streptococcus sp. VT 162 AHZ48483.1     12
 HAS68_18330   Streptococcus suis 05HAS68 AHF60382.1     12
 SSU05_2064   Streptococcus suis 05ZYH33 ABP91030.1 A4VY41   12
 AN924_12530   Streptococcus suis 90-1330 AMU79709.1     12
 SSU98_2067   Streptococcus suis 98HAH33 ABP93225.1 A4W4D6   12
 SSUA7_1881   Streptococcus suis A7 AER45198.1     12
 SSUBM407_1919   Streptococcus suis BM407 CAZ56775.1 C6GXW3   12
 SSUD12_2036   Streptococcus suis D12 AER20299.1     12
 SSUD9_2082   Streptococcus suis D9 AER18240.1     12
 SSGZ1_1875   Streptococcus suis GZ1 ADE32331.1 D5AET8   12
 SSUJS14_2019   Streptococcus suis JS14 ADV71067.1     12
 bifunctional amylopullulanase (ApuA;SSU1849) 3.2.1.1
3.2.1.41
Streptococcus suis P1/7 CAR47543.1 C5VVZ9   12
 YYK_08920   Streptococcus suis S735 AFR01291.1     12
 NJAUSS_1903   Streptococcus suis SC070731 AGG65362.1     12
 B9H01_09920   Streptococcus suis SC19 ARL71004.1     12
 SSUSC84_1871   Streptococcus suis SC84 CAZ52647.1 C6GSP9   12
 SSU12_1998   Streptococcus suis SS12 AER16173.1     12
 SSUST1_1962   Streptococcus suis ST1 AER22304.1     12
 SSUST3_1908   Streptococcus suis ST3 AEB82319.1     12
 T15_2129   Streptococcus suis T15 AGZ24199.1     12
 TL13_1866   Streptococcus suis TL13 AGL48789.1     12
 YB51_9480   Streptococcus suis YB51 AGW88268.1     12
 B1761_00805 (fragment)   Streptococcus thermophilus APC151 AQW32990.1     12
 B1761_00800 (fragment)   Streptococcus thermophilus APC151 AQW32989.1     12
 B1761_00795 (fragment)   Streptococcus thermophilus APC151 AQW32988.1     12
 B1761_00810 (fragment)   Streptococcus thermophilus APC151 AQW32991.1     12
 T303_06155 (fragment)   Streptococcus thermophilus ASCC 1275 AIC25142.1     12
 T303_06150 (fragment)   Streptococcus thermophilus ASCC 1275 AIC24466.1     12
 T303_06160 (fragment)   Streptococcus thermophilus ASCC 1275 AIC25143.1     12
 CG712_01445 (fragment)   Streptococcus thermophilus B59671 ATH76038.1     12
 str1032 (fragment)   Streptococcus thermophilus CNRZ1066 AAV62610.1     12
 BAY21_09360 (fragment)   Streptococcus thermophilus CS8 ANS62222.1     12
 STH8232_1226 (fragment)   Streptococcus thermophilus JIM 8232 CCC19920.1     12
 STH8232_1227 (fragment)   Streptococcus thermophilus JIM 8232 CCC19921.1     12
 STH8232_1228 (fragment)   Streptococcus thermophilus JIM 8232 CCC19922.1     12
 BEN15_08255 (fragment)   Streptococcus thermophilus KLDS 3.1003 AOD27394.1     12
 BEN15_08270 (fragment)   Streptococcus thermophilus KLDS 3.1003 AOD27037.1     12
 BEN15_08265 (fragment)   Streptococcus thermophilus KLDS 3.1003 AOD27036.1     12
 A9497_01325 (fragment)   Streptococcus thermophilus KLDS SM ANJ62050.1     12
 A9497_01320 (fragment)   Streptococcus thermophilus KLDS SM ANJ62049.1     12
 A9497_01315 (fragment)   Streptococcus thermophilus KLDS SM ANJ63505.1     12
 A9497_01330 (fragment)   Streptococcus thermophilus KLDS SM ANJ62051.1     12
 stu1033 (fragment)   Streptococcus thermophilus LMG 18311 AAV60690.1     12
 stu1031 (fragment)   Streptococcus thermophilus LMG 18311 AAV60688.1     12
 AMD33_03480 (fragment)   Streptococcus thermophilus MN-BM-A01 ALD16897.1     12
 AMD33_03470 (fragment)   Streptococcus thermophilus MN-BM-A01 ALD16895.1     12
 AMD33_03475 (fragment)   Streptococcus thermophilus MN-BM-A01 ALD16896.1     12
 MNA02_1005 (fragment)   Streptococcus thermophilus MN-BM-A02 AKH33458.1     12
 MNA02_1004 (fragment)   Streptococcus thermophilus MN-BM-A02 AKH33457.1     12
 Y1U_C0875 (fragment)   Streptococcus thermophilus MN-ZLW-002 AFJ83324.1     12
 STND_0981 (fragment)   Streptococcus thermophilus ND03 ADQ63033.1 E4SP35   12
 BBD27_1796 (fragment)   Streptococcus thermophilus ND07 AOZ59880.1     12
 BBD27_1797 (fragment)   Streptococcus thermophilus ND07 AOZ59881.1     12
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