Glycoside Hydrolase Family 1

Known Activities	b-glucosidase (EC 3.2.1.21); b-galactosidase (EC 3.2.1.23); b-mannosidase (EC 3.2.1.25); b-glucuronidase (EC 3.2.1.31); b-D-fucosidase (EC 3.2.1.38); phlorizin hydrolase (EC 3.2.1.62); exo-b-1,4-glucanase (EC 3.2.1.74); 6-phospho-b-galactosidase (EC 3.2.1.85); 6-phospho-b-glucosidase (EC 3.2.1.86); strictosidine b-glucosidase (EC 3.2.1.105); lactase (EC 3.2.1.108); amygdalin b-glucosidase (EC 3.2.1.117); prunasin b-glucosidase (EC 3.2.1.118); raucaffricine b-glucosidase (EC 3.2.1.125); thioglucosidase (EC 3.2.1.147); b-primeverosidase (EC 3.2.1.149); isoflavonoid 7-O-b-apiosyl-b-glucosidase (EC 3.2.1.161); hydroxyisourate hydrolase (EC 3.-.-.-); b-glycosidase (EC 3.2.1.-)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	(b/a)8
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental); absent in plant myrosinases
External resources	CAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (6076); Uniprot accession (2269); PDB accession (178); 3D entries (39); cryst (0)

Summary

All (4496) Archaea (83) Bacteria (3705) Eukaryota (697) unclassified (11) Structure (39 ) Characterized (267)

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Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-mannosidase (BglB;PF0356)	3.2.1.25	Pyrococcus furiosus DSM 3638	AAL80480.1 NP_578085.1	Q8U3U9
β-mannosidase (BmnA;PF1208) (Man1)	3.2.1.25	Pyrococcus furiosus DSM 3638	AAL81332.1 ABC06395.1 NP_578937.1	E7FHC0
β-glucosidase / β-glycosidase / β-rutinosidase (Cel1A;CelB;BGLPf;PFTG;PF0073;Pf-β-gly)	3.2.1.21 3.2.1.-	Pyrococcus furiosus DSM 3638	AAC25555.1 AAL80197.1 ABC06396.1 NP_577802.1	E7FHY4 Q51723	3APG[A,B,C,D]
β-glucosidase (BglA;PF0442) (Cel1B)	3.2.1.21	Pyrococcus furiosus DSM 3638	AAG28457.1 AAL80566.1 NP_578171.1	E7FI48 Q9HHB3
β-mannosidase (BmnA)	3.2.1.25	Pyrococcus furiosus DSM 3638 VC1	AAC44387.1 AAM56789.1 CAH68690.1 CAT81449.1	E7FHC0 Q51733
alkyl β-glucosidase (membrane-bound) (BGPh;BglA;PH0366)	3.2.1.21	Pyrococcus horikoshii OT3	ABC06393.1 BAA29440.1 NP_142340.1	O58104	1VFF[A]
β-mannosidase (BglB;PH0501)	3.2.1.25	Pyrococcus horikoshii OT3	ABC06394.1 BAA29589.1 NP_142473.1	O58237
β-galactosidase	3.2.1.23	Pyrococcus woesei	AAB97862.1	O52629
β-glycosidase (BgaS;Saci_1849)	3.2.1.-	Sulfolobus acidocaldarius DSM 639	AAY81155.1 YP_256448.1	P14288
β-glycosidase (Bgly;SSG)	3.2.1.-	Sulfolobus shibatae	AAA79030.1	P50388
β-glycosidase (LacS;Ssol98_010100014373)	3.2.1.21 3.2.1.23	Sulfolobus solfataricus 98/2	AAD21094.1 AET42942.1	G8GCT7
β-glycosidase / β-galactosidase	3.2.1.-	Sulfolobus solfataricus DSM1616	CAA34074.1	P14288
β-glycosidase S (LacS;Ss-β-gly;SSO3019)	3.2.1.21 3.2.1.23 3.2.1.-	Sulfolobus solfataricus P2	AAA72843.1 AAE29896.1 AAK43121.1 ABC06397.1 ACW34039.1 CAC24042.1 CAJ18790.1 NP_344331.1	P22498	1GOW[A,B] 1UWI[A,B,C,D] 1UWQ[A,B] 1UWR[A,B] 1UWS[A,B] 1UWT[A,B] 1UWU[A,B] 2CEQ[A,B] 2CER[A,B] 4EAM[A,B] 4EAN[A,B]
β-glycosidase (TK1827)	3.2.1.-	Thermococcus kodakarensis KOD1	BAD86016.1 CAT70991.1	Q5JDV2
β-glycosidase (TK1761)	3.2.1.-	Thermococcus kodakarensis KOD1	BAA78713.1 BAD85950.1 CAT70557.1	Q9YGB8
β-glucosidase (BglT)	3.2.1.21	Thermococcus sp. AL662	CAA94187.1	O08324
TVG0691226	3.2.1.23	Thermoplasma volcanium GSS1	BAB59827.1 NP_111204.1	Q97AX4
β-glycosidase (Tα-β-gly)	3.2.1.-	Thermosphaera aggregans M11TL	AAD43138.1 AAM56782.1	Q9YGA8	1QVB[A,B]
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-glucosidase (Abg)	3.2.1.21	Agrobacterium sp. ATCC21400	AAA22085.1 AAQ79763.1	P12614
β-glycosidase (GlyB;Aaci_1895)	3.2.1.-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	AAZ81839.1 ACV58907.1	C8WXU5
β-glucosidase (BglA)	3.2.1.21	Bacillus circulans subsp. alkalophilus	AAA22266.1 AAQ79761.1 CBG21249.1	Q03506	1QOX[A]
β-glucosidase (BglA;BH1923)	3.2.1.21	Bacillus halodurans C-125	BAB05642.1 CBG20998.1 NP_242789.1	Q9KBK3
β-glucosidase A	3.2.1.21	Bacillus sp. GL1	BAA36160.1 CBG21242.1	Q9ZNN7
6-P-β-glucosidase (YdhP;BSU05840)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	BAA19708.1 CAB12403.1 CBG21028.1 NP_388465.1	O05508
6-P-β-glucosidase (BglA;BSU40110)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	AAA22660.1 BAA11270.1 CAB16048.1 CBG21250.1 NP_391891.1	P42973
6-P-β-glucosidase 1 (BglH;N17D;BSU39260)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	CAA84287.1 CAB15962.2 CBG21247.1 NP_391805.1	P40740
6-P-β-glucosidase (YckE;BglC;BSU03410)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	BAA06429.1 BAA08975.1 CAB12135.1 CBG21248.1 NP_388223.1	P42403
β-glucosidase / β-xylosidase (BglB;P11-6B)	3.2.1.21 3.2.1.37	bacterium enrichment culture clone P11-6B	ADT62000.1
β-glucosidase / β-D-fucosidase	3.2.1.21 3.2.1.38	Bifidobacterium breve 203 / clb	BAA19881.1 CBG21199.1	P94248
β-glucosidase / cellodextrinase (CldC; Bbr_0109)	3.2.1.21	Bifidobacterium breve UCC2003	ABE94821.1 ACT78497.1
β-glucosidase (BglS)	3.2.1.21	Caldicellulosiruptor 14B	CAA10354.1
β-glucosidase / cellodextrinase (CbBgl1A;CbCdx1A;Athe_0458) (Bgl1A=Cdx1A)	3.2.1.21	Anaerocellum thermophilum DSM 6725	ACM59590.1	B9MNR1
β-glucosidase A (BglA)	3.2.1.21	Caldicellulosiruptor saccharolyticus	AAQ79759.1 CAA31087.1 CBG21257.1	P10482
β-glucosidase (BglA;Csac_1089)	3.2.1.21	Caldicellulosiruptor saccharolyticus DSM 8903	ABP66702.1	A4XIG7
cellobiase (Cba3)	3.2.1.21	Cellulomonas biazotea ATCC 486	AEM45802.1
β-glucosidase	3.2.1.21	Cellulomonas fimi ATCC 484	AAA23091.1	Q46043
cellodextrin glucohydrolase (Ccel_0374)	3.2.1.21 3.2.1.23 3.2.1.74	Clostridium cellulolyticum H10 ATCC 35319	ACL74759.1	B8I5U2
β-glucosidase A (BglA;BG)	3.2.1.21	Clostridium cellulovorans	AAQ00997.1 CBG21209.1	Q53EH2	3AHX[A,B,C,D]
6-P-β-glucosidase (AbgA)	3.2.1.86	Clostridium longisporum	AAC05714.1	Q46130
exo-β-glucosidase 1A (BglA; β-G;Cthe_0212) (Glc1A)	3.2.1.21	Clostridium thermocellum ATCC 27405	AAQ79758.1 ABN51453.1 CAA42814.1	P26208
β-glucosidase A (BglA;DP2448)	3.2.1.21	Desulfotalea psychrophila LSv54	CAG37177.1 YP_066184.1	Q6AKE8
β-glucosidase (DtGH;DICTH_1689)	3.2.1.21	Dictyoglomus thermophilum H-6-12 ATCC 35947	ACI19973.1	B5YAN1
6-P-β-glucosidase (BglB)	3.2.1.86	Enterococcus faecium BFE 900	AAD28227.1 CBG21239.1	Q9X564
6-P-β-glucosidase (ArbB)	3.2.1.86	Erwinia chrysanthemi 3665	AAA24815.1 AAQ79756.1 CBG21244.1	P26206
6-P-β-glucosidase (BglB;b3721)	3.2.1.86	Escherichia coli K-12 MG1655	AAA23511.1 AAA62072.1 AAA83838.1 AAC76744.1 AAQ79757.1 NP_418177.1	P11988
6-P-β-glucosidase 2 (AscB)	3.2.1.86	Escherichia coli K-12 MG1655	AAA16430.1 AAA69226.1 AAC75758.1 NP_417196.1	P24240
6-P-β-glucosidase A (BglA;b2901)	3.2.1.86	Escherichia coli K-12 MG1655	AAA83082.1 AAC75939.1 CBG20988.1 NP_417377.1	Q46829	2XHY[A,B,C,D]
β-glucosidase (BglA)	3.2.1.21	Exiguobacterium sp. DAU5	ADK47980.1	E0X9H4
β-glucosidase (FiBgl1A) (Bgl1A)	3.2.1.21	Fervidobacterium islandicum	CCA60742.1
β-glucosidase (BglA)	3.2.1.21	Fervidobacterium sp. YNP	AAN60220.1	Q8GKQ8
6-P-β-glucosidase (PbgA)	3.2.1.86	Fusobacterium mortiferum	AAB49339.1	P94793
6-phospho-β-glycosidase (GK3214)	3.2.1.85 3.2.1.86	Geobacillus kaustophilus HTA426	BAD77499.1 CBG21169.1	Q5KUY7
6-phospho-β-glycosidase (GK1856)	3.2.1.85 3.2.1.86	Geobacillus kaustophilus HTA426	BAD76141.1 CBG21167.1	Q5KYU5
β-glucosidase A (BglA;Hore_15280)	3.2.1.21	Halothermothrix orenii H 168 H 168; DSM 9562	ACL70277.1	B8CYA8	3TA9[A,B]
6-P-β-glucosidase / phospho-cellobiase (CasB)	3.2.1.86	Klebsiella oxytoca P2	AAB51564.1 CBG21258.1	Q48409
6-P-β-galactosidase	3.2.1.85	Lactobacillus acidophilus	BAA07122.1 CBG21259.1	P50977
6-P-β-galactosidase	3.2.1.85	Lactobacillus casei	AAA25238.1 AAD15134.1 AAQ79754.1 CAB02557.1 CBG21260.1	P14696
6-P-β-glucosidase (Pbg1;LGAS_0185)	3.2.1.86	Lactobacillus gasseri ATCC 33323	ABJ59596.1	Q046M6
6-P-β-galactosidase (LacG1;LGAS_0344)	3.2.1.85	Lactobacillus gasseri ATCC 33323	ABJ59750.1	Q045X2
6-P-β-galactosidase (LacG2;LGAS_0502)	3.2.1.85	Lactobacillus gasseri ATCC 33323	ABJ59900.1	Q045H2
6-P-β-glucosidase (Pbg2;LGAS_1668)	3.2.1.86	Lactobacillus gasseri ATCC 33323	ABJ60960.1	Q041B2
6-P-β-galactosidase 1 (Pbg1)	3.2.1.86	Lactobacillus gasseri JCM1031	BAA20086.1 BAD95552.1 CBG21210.1	O06532
6-P-β-galactosidase II (Pbg2)	3.2.1.86	Lactobacillus gasseri JCM1031	BAA25004.1 CBG21197.1	O50658
aryl 6-P-β-glucosidase (BglH;BglT)	3.2.1.86	Lactobacillus plantarum B21	CAA75906.1	O86291
6-P-β-galactosidase (LacG)	3.2.1.85	Lactococcus lactis Z268	AAA25173.1 AAA25183.1 AAA26949.1 AAQ79752.1 CBG21261.1	P11546	1PBG[A,B] 2PBG[A] 3PBG[A,B] 4PBG[A,B]
β-glucosidase (BglM1;Mmwyl1_4295) (cold-active)	3.2.1.21	Marinomonas sp. MWYL1	ABR73190.1	A6W3B1
β-glucosidase (Bgl;BglU)	3.2.1.21	Micrococcus antarcticus	ACM66669.1	B9V8P5
6-P-β-glucosidase (BglD;OEOE_0224)	3.2.1.86	Oenococcus oeni PSU-1	ABJ56211.1	Q04H61
6P-β-glucosidase (CelD;OEOE_0341)	3.2.1.86	Oenococcus oeni PSU-1	ABJ56315.1	Q04GV7
β-glucosidase A (BglA)	3.2.1.21	Paenibacillus polymyxa	AAA22263.1 AAQ79760.1 CBG21252.1	P22073	1BGA[A,B,C,D] 1BGG[A,B,C,D] 1E4I[A] 1TR1[A,B,C,D] 1UYQ[A]
β-glucosidase B (BglB)	3.2.1.21	Paenibacillus polymyxa	AAA22264.1 AAQ79762.1 CBG21256.1	P22505	2JIE[A] 2O9P[A] 2O9R[A] 2O9T[A] 2Z1S[A]
β-glucosidase (BglA)	3.2.1.21	Paenibacillus sp. HC1	BAE48718.1	Q2WGB4
β-1,4-glucosidase (Gluc1C)	3.2.1.21	Paenibacillus sp. ICGEB2008 / MTCC 5639	AFQ36783.1
β-glucosidase A	3.2.1.21	Pantoea agglomerans WD4 WILD STRAIN	CAA56282.1	Q59437
6-P-β-glucosidase (AscB)	3.2.1.21 3.2.1.86	Pectobacterium carotovorum subsp. carotovorum LY34	AAT45375.1	Q6IV14
6-P-β-glucosidase (BglA)	3.2.1.21 3.2.1.86	Pectobacterium carotovorum subsp. carotovorum LY34	AAV37466.1	Q5UAU4
β-glucosidase (CasB)	3.2.1.21	Pectobacterium carotovorum subsp. carotovorum LY34	AAX57580.1	Q4VK76
β-glucosidase (BglY)	3.2.1.21	Pectobacterium carotovorum subsp. carotovorum LY34	AAX76617.1	Q2TI56
β-glucosidase (CelG)	3.2.1.21	Pectobacterium carotovorum subsp. carotovorum LY34	ABL14155.1	A1C3J9
β-glucosidase 1B (Sde_1394) (Bgl1B)	3.2.1.21	Saccharophagus degradans 2-40	ABD80656.1	Q21KX3
β-glucosidase 1A (Sde_3603) (Bgl1A)	3.2.1.21	Saccharophagus degradans 2-40	ABD82858.1	Q21EM1
β-glucosidase (SMc03160)	3.2.1.21	Sinorhizobium meliloti 1021	ADA60163.1 CAC47470.1 NP_386997.1	Q92LX2
β-glucosidase (Bgl1)	3.2.1.21	Sphingomonas paucimobilis	AAG59862.1	Q9APE3
β-glucosidase (BglSp)	3.2.1.21	Sphingomonas sp. 2F2	ADY18331.1
6-P-β-galactosidase (SAV2189)	3.2.1.85	Staphylococcus aureus subsp. aureus Mu50	AAA26650.1 AAQ79753.1 BAB45117.1 BAB58351.1 CBG21263.1 NP_372713.1	P11175
6-P-β-glucosidase (SAV0266;BglA)	3.2.1.86	Staphylococcus aureus subsp. aureus Mu50	BAB56428.1 CBG21027.1 NP_370790.1	Q99WV9
6-P-β-glucosidase (BglA;MW0242)	3.2.1.86	Staphylococcus aureus subsp. aureus MW2	BAB94107.1 NP_645057.1	Q8NYG9
6-P-β-galactosidase (LacG;SGO_1512)	3.2.1.85	Streptococcus gordonii str. Challis substr. CH1	AAG39001.1 ABV10357.1 CBG21234.1	Q9EV38
β-glucosidase (BglA)	3.2.1.86	Streptococcus mutans NG8	AAF89979.1 CBG21238.1	Q9KJ76
6-P-β-galactosidase (LacG)	3.2.1.85	Streptococcus mutans UA130	AAA16450.1 CBG21265.1	P50978
6-P-β-glucosidase (BglA;SMU.985)	3.2.1.86	Streptococcus mutans UA159	AAN58686.1 CBG21066.1 NP_721380.1
6-P-β-glucosidase (Arb;AscB;SMU.1102)	3.2.1.86	Streptococcus mutans UA159	AAN58797.1 CBG21067.1 NP_721491.1
6-P-β-galactosidase (LacG;SMU.1490)	3.2.1.85	Streptococcus mutans UA159	AAN59144.1 NP_721838.1
6-P-β-glucosidase (CelA;Bgl;SmBgl;SMU.1601;SMU_1601)	3.2.1.86	Streptococcus mutans UA159	AAN59243.1 CBG21068.1 NP_721937.1		3PN8[A,B] 4DDE[A,B] 4F66[A,B] 4F79[A] 4GPN[A,B]
6-P-β-glucosidase (BglA-2;SP0578)	3.2.1.86	Streptococcus pneumoniae TIGR4	AAK74732.1 CBG21011.1 NP_345092.1	Q97S37	4IPL[A,B] 4IPN[B,E]
6-phospho-β-glucosidase (SPy_1599;SPy1599)	3.2.1.86	Streptococcus pyogenes M1 GAS SF370	AAK34377.1 NP_269656.1	Q99YP9	4B3K[A,B,C,D,E,F] 4B3L[A,B,C,D,E,F]
β-glucosidase (Bgsl)	3.2.1.21	Streptomyces rochei A2	CAA52344.1	Q55000
β-glucosidase (Bgl3)	3.2.1.21	Streptomyces sp. QM-B814	CAA82733.1 CBG21211.1	Q59976	1GNX[A,B] 1GON[A,B]
β-glucosidase (CglT;BglT)	3.2.1.21	Thermoanaerobacter brockii ATCC 53556 / DSM 1457	CAA91220.1 CBG21215.1	Q60026
β-glucosidase (BglA)	3.2.1.21	Thermoanaerobacter ethanolicus JW 200	ADD25173.1	D3Y2V4
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Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.