Glycoside Hydrolase Family 1

Known Activities	β-glucosidase (EC 3.2.1.21); β-galactosidase (EC 3.2.1.23); β-mannosidase (EC 3.2.1.25); β-glucuronidase (EC 3.2.1.31); β-D-fucosidase (EC 3.2.1.38); phlorizin hydrolase (EC 3.2.1.62); exo-β-1,4-glucanase (EC 3.2.1.74); 6-phospho-β-galactosidase (EC 3.2.1.85); 6-phospho-β-glucosidase (EC 3.2.1.86); strictosidine β-glucosidase (EC 3.2.1.105); lactase (EC 3.2.1.108); amygdalin β-glucosidase (EC 3.2.1.117); prunasin β-glucosidase (EC 3.2.1.118); raucaffricine β-glucosidase (EC 3.2.1.125); thioglucosidase (EC 3.2.1.147); β-primeverosidase (EC 3.2.1.149); isoflavonoid 7-O-β-apiosyl-β-glucosidase (EC 3.2.1.161); hydroxyisourate hydrolase (EC 3.-.-.-); β-glycosidase (EC 3.2.1.-)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) ₈
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental); absent in plant myrosinases
External resources	CAZypedia; HOMSTRAD; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (5042); Uniprot accession (2187); PDB accession (133); 3D entries (35); cryst (1)

Summary

All (3511) Archaea (72) Bacteria (2808) Eukaryota (624) unclassified (7) Structure (35 - 1 cryst) Characterized (231)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-mannosidase (BglB;PF0356)	3.2.1.25	Pyrococcus furiosus DSM 3638	AAL80480.1 NP_578085.1	Q8U3U9
β-mannosidase (BmnA;PF1208) (Man1)	3.2.1.25	Pyrococcus furiosus DSM 3638	AAL81332.1 ABC06395.1 NP_578937.1
β-glucosidase / β-glycosidase / β-rutinosidase (CelB;BGLPf;PF0073;Pf-β-gly) (Cel1A)	3.2.1.- 3.2.1.21	Pyrococcus furiosus DSM 3638	AAC25555.1 AAL80197.1 ABC06396.1 NP_577802.1	Q51723	3APG[A,B,C,D]
β-glucosidase (BglA;PF0442) (Cel1B)	3.2.1.21	Pyrococcus furiosus DSM 3638	AAG28457.1 AAL80566.1 NP_578171.1	Q9HHB3
β-mannosidase (BmnA)	3.2.1.25	Pyrococcus furiosus DSM 3638 VC1	AAC44387.1 AAM56789.1 CAH68690.1 CAT81449.1	Q51733
alkyl β-glucosidase (membrane-bound) (BGPh;BglA;PH0366)	3.2.1.21	Pyrococcus horikoshii OT3	ABC06393.1 BAA29440.1 NP_142340.1	O58104	1VFF[A]
β-mannosidase (BglB;PH0501)	3.2.1.25	Pyrococcus horikoshii OT3	ABC06394.1 BAA29589.1 NP_142473.1	O58237
β-galactosidase	3.2.1.23	Pyrococcus woesei	AAB97862.1	O52629.1
β-glycosidase (BgaS;Saci_1849)	3.2.1.-	Sulfolobus acidocaldarius DSM 639	AAY81155.1 YP_256448.1
β-glycosidase (Bgly;SSG)	3.2.1.-	Sulfolobus shibatae	AAA79030.1	P50388
β-glycosidase (LacS;Ssol98_010100014373)	3.2.1.21 3.2.1.23	Sulfolobus solfataricus 98/2	AAD21094.1 AET42942.1
β-galactosidase	3.2.1.23	Sulfolobus solfataricus DSM1616	CAA34074.1	P14288.2
β-glycosidase S (LacS;Ss-β-gly;SSO3019)	3.2.1.- 3.2.1.21 3.2.1.23	Sulfolobus solfataricus P2	AAA72843.1 AAE29896.1 AAK43121.1 ABC06397.1 ACW34039.1 CAC24042.1 CAJ18790.1 NP_344331.1	P22498.2	1GOW[A,B] 1UWI[A,B,C,D] 1UWQ[A,B] 1UWR[A,B] 1UWS[A,B] 1UWT[A,B] 1UWU[A,B] 2CEQ[A,B] 2CER[A,B]
β-glycosidase (TK1827)	3.2.1.-	Thermococcus kodakarensis KOD1	BAD86016.1 CAT70991.1	Q5JDV2
β-glycosidase (TK1761)	3.2.1.-	Thermococcus kodakarensis KOD1	BAA78713.1 BAD85950.1 CAT70557.1	Q9YGB8
β-glucosidase (BglT)	3.2.1.21	Thermococcus sp. AL662	CAA94187.1	O08324
TVG0691226	3.2.1.23	Thermoplasma volcanium GSS1	BAB59827.1 NP_111204.1	Q97AX4
β-glycosidase (Tα-β-gly)	3.2.1.-	Thermosphaera aggregans M11TL	AAD43138.1 AAM56782.1	Q9YGA8	1QVB[A,B]
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-glucosidase (Abg)	3.2.1.21	Agrobacterium sp. ATCC21400	AAA22085.1 AAQ79763.1	P12614.1
β-glycosidase (GlyB;Aaci_1895)	3.2.1.-	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	AAZ81839.1 ACV58907.1	C8WXU5
β-glucosidase (BglA)	3.2.1.21	Bacillus circulans subsp. alkalophilus	AAA22266.1 AAQ79761.1 CBG21249.1	Q03506	1QOX[A]
β-glucosidase (BglA;BH1923)	3.2.1.21	Bacillus halodurans C-125	BAB05642.1 CBG20998.1 NP_242789.1	Q9KBK3
β-glucosidase A	3.2.1.21	Bacillus sp. GL1	BAA36160.1 CBG21242.1	Q9ZNN7
6-P-β-glucosidase (YdhP;BSU05840)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	BAA19708.1 CAB12403.1 CBG21028.1 NP_388465.1	O05508
6-P-β-glucosidase (BglA;BSU40110)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	AAA22660.1 BAA11270.1 CAB16048.1 CBG21250.1 NP_391891.1	P42973
6-P-β-glucosidase 1 (BglH;N17D;BSU39260)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	CAA84287.1 CAB15962.2 CBG21247.1 NP_391805.1	P40740
6-P-β-glucosidase (YckE;BglC;BSU03410)	3.2.1.86	Bacillus subtilis subsp. subtilis str. 168	BAA06429.1 BAA08975.1 CAB12135.1 CBG21248.1 NP_388223.1	P42403
β-glucosidase / β-xylosidase (BglB;P11-6B)	3.2.1.21 3.2.1.37	bacterium enrichment culture clone P11-6B	ADT62000.1
β-glucosidase / β-D-fucosidase	3.2.1.21 3.2.1.38	Bifidobacterium breve 203 / clb	BAA19881.1 CBG21199.1	P94248
β-glucosidase / cellodextrinase (CldC; Bbr_0109)	3.2.1.21	Bifidobacterium breve UCC2003	ABE94821.1 ACT78497.1
β-glucosidase (BglS)	3.2.1.21	Caldicellulosiruptor 14B	CAA10354.1
β-glucosidase A (BglA)	3.2.1.21	Caldicellulosiruptor saccharolyticus	AAQ79759.1 CAA31087.1 CBG21257.1	P10482.1
β-glucosidase (BglA;Csac_1089)	3.2.1.21	Caldicellulosiruptor saccharolyticus DSM 8903	ABP66702.1	A4XIG7
cellobiase (Cba3)	3.2.1.21	Cellulomonas biazotea ATCC 486	AEM45802.1
β-glucosidase	3.2.1.21	Cellulomonas fimi ATCC 484	AAA23091.1	Q46043
cellodextrin glucohydrolase (Ccel_0374)	3.2.1.21 3.2.1.23 3.2.1.74	Clostridium cellulolyticum H10 ATCC 35319	ACL74759.1	B8I5U2
β-glucosidase A (BglA;BG)	3.2.1.21	Clostridium cellulovorans	AAQ00997.1 CBG21209.1	Q53EH2	3AHX[A,B,C,D]
6-P-β-glucosidase (AbgA)	3.2.1.86	Clostridium longisporum	AAC05714.1	Q46130
exo-β-glucosidase 1A (BglA; β-G;Cthe_0212) (Glc1A)	3.2.1.21	Clostridium thermocellum ATCC 27405	AAQ79758.1 ABN51453.1 CAA42814.1	P26208.1
β-glucosidase A (BglA;DP2448)	3.2.1.21	Desulfotalea psychrophila LSv54	CAG37177.1 YP_066184.1
6-P-β-glucosidase (BglB)	3.2.1.86	Enterococcus faecium BFE 900	AAD28227.1 CBG21239.1	Q9X564
6-P-β-glucosidase (ArbB)	3.2.1.86	Erwinia chrysanthemi 3665	AAA24815.1 AAQ79756.1 CBG21244.1	P26206.1
6-P-β-glucosidase (BglB;b3721)	3.2.1.86	Escherichia coli K-12 MG1655	AAA23511.1 AAA62072.1 AAA83838.1 AAC76744.1 AAQ79757.1 NP_418177.1	P11988.2
6-P-β-glucosidase 2 (AscB)	3.2.1.86	Escherichia coli K-12 MG1655	AAA16430.1 AAA69226.1 AAC75758.1 NP_417196.1	P24240.2
6-P-β-glucosidase A (BglA;b2901)	3.2.1.86	Escherichia coli K-12 MG1655	AAA83082.1 AAC75939.1 CBG20988.1 NP_417377.1	Q46829	2XHY[A,B,C,D]
β-glucosidase (BglA)	3.2.1.21	Exiguobacterium sp. DAU5	ADK47980.1	E0X9H4
β-glucosidase (FiBgl1A) (Bgl1A)	3.2.1.21	Fervidobacterium islandicum	CCA60742.1
β-glucosidase (BglA)	3.2.1.21	Fervidobacterium sp. YNP	AAN60220.1	Q8GKQ8
6-P-β-glucosidase (PbgA)	3.2.1.86	Fusobacterium mortiferum	AAB49339.1	P94793
6-P-β-glucosidase / phospho-cellobiase (CasB)	3.2.1.86	Klebsiella oxytoca P2	AAB51564.1 CBG21258.1	Q48409
6-P-β-galactosidase	3.2.1.85	Lactobacillus acidophilus	BAA07122.1 CBG21259.1	P50977
6-P-β-galactosidase	3.2.1.85	Lactobacillus casei	AAA25238.1 AAD15134.1 AAQ79754.1 CAB02557.1 CBG21260.1	P14696.1
6-P-β-galactosidase 1 (Pbg1)	3.2.1.86	Lactobacillus gasseri JCM1031	BAA20086.1 BAD95552.1 CBG21210.1	O06532
6-P-β-galactosidase II (Pbg2)	3.2.1.86	Lactobacillus gasseri JCM1031	BAA25004.1 CBG21197.1	O50658
aryl 6-P-β-glucosidase (BglH;BglT)	3.2.1.86	Lactobacillus plantarum B21	CAA75906.1	O86291
6-P-β-galactosidase (LacG)	3.2.1.85	Lactococcus lactis Z268	AAA25173.1 AAA25183.1 AAA26949.1 AAQ79752.1 CBG21261.1	P11546.2	1PBG[A,B] 2PBG[A] 3PBG[A,B] 4PBG[A,B]
β-glucosidase (BglM1;Mmwyl1_4295) (cold-active)	3.2.1.21	Marinomonas sp. MWYL1	ABR73190.1	A6W3B1
β-glucosidase (Bgl;BglU)	3.2.1.21	Micrococcus antarcticus	ACM66669.1	B9V8P5
6-P-β-glucosidase (BglD;OEOE_0224)	3.2.1.86	Oenococcus oeni PSU-1	ABJ56211.1	Q04H61
6P-β-glucosidase (CelD;OEOE_0341)	3.2.1.86	Oenococcus oeni PSU-1	ABJ56315.1	Q04GV7
β-glucosidase A (BglA)	3.2.1.21	Paenibacillus polymyxa	AAA22263.1 AAQ79760.1 CBG21252.1	P22073.1	1BGA[A,B,C,D] 1BGG[A,B,C,D] 1E4I[A] 1TR1[A,B,C,D] 1UYQ[A]
β-glucosidase B (BglB)	3.2.1.21	Paenibacillus polymyxa	AAA22264.1 AAQ79762.1 CBG21256.1	P22505.1	2JIE[A] 2O9P[A] 2O9R[A] 2O9T[A] 2Z1S[A]
β-glucosidase (BglA)	3.2.1.21	Paenibacillus sp. HC1	BAE48718.1	Q2WGB4
β-glucosidase A	3.2.1.21	Pantoea agglomerans WD4 WILD STRAIN	CAA56282.1	Q59437
β-glucosidase (CelG)	3.2.1.21	Pectobacterium carotovorum subsp. carotovorum LY34	ABL14155.1	A1C3J9
β-glucosidase 1B (Sde_1394) (Bgl1B)	3.2.1.21	Saccharophagus degradans 2-40	ABD80656.1	Q21KX3
β-glucosidase 1A (Sde_3603) (Bgl1A)	3.2.1.21	Saccharophagus degradans 2-40	ABD82858.1	Q21EM1
β-glucosidase (SMc03160)	3.2.1.21	Sinorhizobium meliloti 1021	CAC47470.1 NP_386997.1	Q92LX2
β-glucosidase (Bgl1)	3.2.1.21	Sphingomonas paucimobilis	AAG59862.1	Q9APE3
β-glucosidase (BglSp)	3.2.1.21	Sphingomonas sp. 2F2	ADY18331.1
6-P-β-galactosidase (SAV2189)	3.2.1.85	Staphylococcus aureus subsp. aureus Mu50	AAA26650.1 AAQ79753.1 BAB45117.1 BAB58351.1 CBG21263.1 NP_372713.1	P11175.1
6-P-β-glucosidase (SAV0266;BglA)	3.2.1.86	Staphylococcus aureus subsp. aureus Mu50	BAB56428.1 CBG21027.1 NP_370790.1	Q99WV9
6-P-β-glucosidase (BglA;MW0242)	3.2.1.86	Staphylococcus aureus subsp. aureus MW2	BAB94107.1 NP_645057.1	Q8NYG9
6-P-β-galactosidase (LacG)	3.2.1.85	Streptococcus gordonii V288	AAG39001.1 CBG21234.1	Q9EV38
β-glucosidase (BglA)	3.2.1.86	Streptococcus mutans NG8	AAF89979.1 CBG21238.1	Q9KJ76
6-P-β-galactosidase (LacG)	3.2.1.85	Streptococcus mutans UA130	AAA16450.1 CBG21265.1	P50978
6-P-β-glucosidase (BglA;SMU.985)	3.2.1.86	Streptococcus mutans UA159	AAN58686.1 CBG21066.1 NP_721380.1
6-P-β-glucosidase (Arb;AscB;SMU.1102)	3.2.1.86	Streptococcus mutans UA159	AAN58797.1 CBG21067.1 NP_721491.1
6-phospho-β-galactosidase (LacG;SMU.1490)	3.2.1.85	Streptococcus mutans UA159	AAN59144.1 NP_721838.1
6-P-β-glucosidase (CelA;Bgl;SMU.1601)	3.2.1.86	Streptococcus mutans UA159	AAN59243.1 CBG21068.1 NP_721937.1		3PN8[A,B]
β-glucosidase (Bgl3)	3.2.1.21	Streptomyces sp. QM-B814	CAA82733.1 CBG21211.1	Q59976	1GNX[A,B] 1GON[A,B]
β-glucosidase (CglT)	3.2.1.21	Thermoanaerobacter brockii	CAA91220.1 CBG21215.1	Q60026
β-glucosidase (BglA)	3.2.1.21	Thermoanaerobacter ethanolicus JW 200	ADD25173.1	D3Y2V4
β-glucosidase (BglC) (Bgl1C)	3.2.1.21	Thermobifida fusca ER1	AAF37730.1	Q9LAV5
β-glucosidase (BglC;BGL;Tfu_0937) (Bgl1C)	3.2.1.21	Thermobifida fusca YX	AAZ54975.1 CBG21191.1	Q47RE2
β-glucosidase B (BglB)	3.2.1.21	Thermobispora bispora	AAA25311.1	P38645
β-glucosidase A (BglA)	3.2.1.21	Thermotoga maritima MSB8	AEP25088.1 CAA52276.1 CBG21253.1	Q08638	1OD0[A,B] 1OIF[A,B] 1OIM[A,B] 1OIN[A,B] 1UZ1[A,B] 1W3J[A,B] 2CBU[A,B] 2CBV[A,B] 2CES[A,B] 2CET[A,B] 2J75[A,B] 2J77[A,B] 2J78[A,B] 2J79[A,B] 2J7B[A,B] 2J7C[A,B] 2J7D[A,B] 2J7E[A,B] 2J7F[A,B] 2J7G[A,B] 2J7H[A,B] 2JAL[A,B] 2VRJ[A,B] 2WBG[A,B,C,D] 2WC3[A,B,C,D] 2WC4[A,B,C,D]
β-glucosidase A / 1A (BglA;GghA;CTN_0782) (Bgl1A)	3.2.1.21	Thermotoga neapolitana DSM 4359	AAB95492.2 ACM22958.1 CAB10165.1 CBG21254.1	B9K7M5 O33843.2
β-glucosidase (BG-A;TCel12;Tpet_0952)	3.2.1.21	Thermotoga petrophila RKU-1	ABQ46970.1	A5IL97
β-glycosidase / β-glucosidase (BglT;BglA)	3.2.1.- 3.2.1.21	Thermus caldophilus GK24	AAO15361.1	Q8GHE5
β-glycosidase (tfi β-gly)	3.2.1.-	Thermus filiformis WAI33 A1	AAN05440.1	Q8GEB2
β-glycosidase (Gly)	3.2.1.21	Thermus nonproteolyticus HG102	AAF36392.1	Q9L794	1NP2[A,B]
β-glycosidase (BglA)	3.2.1.-	Thermus sp. IB-21	AAN05441.1	Q8GEB1
β-glycosidase (BglB)	3.2.1.-	Thermus sp. IB-21	AAN05442.1	Q8GEB0
β-glucosidase	3.2.1.21	Thermus sp. Z-1	BAA86923.1	Q9RA58
β-glycosidase	3.2.1.-	Thermus thermophilus AT-62	AAN05438.1	Q8GEB4
β-glycosidase (BglT;B-gly;TTP0042)	3.2.1.-	Thermus thermophilus HB27	AAS82372.1 CBG21120.1	Q746L1
β-glycosidase (β-gly)	3.2.1.-	Thermus thermophilus HJ6	ABW87307.1	A8WAC9
β-glycosidase (β-gly)	3.2.1.21 3.2.1.23	Thermus thermophilus KNOUC202	ABI35984.1	Q0GA07
β-glycosidase (BglT;B-gly)	3.2.1.-	Thermus thermophilus TH125	AAD32630.2 CAB42553.2	Q9RA61
β-glucosidase (Bgl1B;PB47B2)	3.2.1.21	uncultured bacterium	ACY09072.1	D0VEC8
β-glucosidase (PB42H12) (Bgl1A)	3.2.1.21	uncultured bacterium	ADD96762.1	D5KX75
β-glycosidase / β-glucosidase (BglA) (Bgl1A)	3.2.1.- 3.2.1.21	uncultured bacterium	ABI18350.1	Q0GMU3	3CMJ[A] 3FIY[A] 3FIZ[A] 3FJ0[A]
6-P-β-glucosidase (BglA;YPO1254)	3.2.1.86	Yersinia pestis CO92 Biovar Orientalis	CAC90089.1 CAL19912.1 NP_404854.1	Q8ZGN2
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
At3g03640 (β-glucosidase;Gluc;Bglu25)	3.2.1.21	Arabidopsis thaliana	AAC31962.1 AAC32194.1 AAF03468.1 AEE73966.1 NP_187014.1	O82772
ER β-glucosidase (Psr3.2;Bglu21;At1g66270)	3.2.1.21	Arabidopsis thaliana	AAB64244.1 AAG51761.1 AAG52157.1 AAK74056.1 AAO11600.1 AEE34488.1 AEE34489.1 NP_176801.1 NP_849848.1	O23656 Q9C525
At5g26000 (myrosinase;TGG1;Bglu38)	3.2.1.147	Arabidopsis thaliana	AAC18869.1 AAD40143.1 AAK74039.1 AAL25596.1 AAL91284.1 AAN60236.1 AED93511.1 AED93512.1 BAH20253.1 CAA55786.1 CAH40799.1 CAH40800.1 CAH40801.1 CAH40802.1 CAH40803.1 CAH40804.1 CAH40805.1 CAH40806.1 CAH40807.1 CAH40808.1 CAH40809.1 CAH40810.1 CAH40811.1 CAH40812.1 CAH40813.1 CAH40814.1 CAH40815.1 CAH40816.1 CAH40817.1 CAH40818.1 CAH40819.1 CAH40820.1 CAH40821.1 CAH40822.1 CAH40823.1 CAH40824.1 CAH40825.1 CAH40826.1 NP_197972.1 NP_851077.1	P37702.1
myrosinase 2 (TGG2;Bglu37;At5g25980) (leaf)	3.2.1.147	Arabidopsis thaliana	AAD40134.1 AAK28645.1 AAK32833.1 AAL77743.1 AAM44928.1 AAN60275.1 AED93507.1 AED93508.1 BAD94532.1 BAD94810.1 BAE98479.1 CAA55787.1 NP_568479.1	Q42595 Q8H7E3 Q9ASV0 Q9C5C2
myrosinase (TGG4;Bglu34;At1g47600)	3.2.1.147	Arabidopsis thaliana	AAD46026.1 AAN17448.1 AAN72213.1 AAO00818.1 ACO95139.1 AEE32191.1 AEE32192.1 NP_175191.2	Q8GRX1 Q9SX92
At1g61810 (monolignol β-glucosidase;Bglu45)	3.2.1.21	Arabidopsis thaliana	AAC28501.1 AEE33889.1 AEE33890.1 AEE33891.1 NP_176374.1	O80689
At1g61820 (monolignol β-glucosidase;Bglu46)	3.2.1.21	Arabidopsis thaliana	AAC28502.1 AAN60285.1 AAU05454.1 AAU45206.1 AEE33892.1 AEE33893.1 NP_176375.1	O80690 Q8H7D5
ER β-glucosidase (Pyk10;Bglu23;Psr3.1;At3g09260)	3.2.1.21	Arabidopsis thaliana	AAB38783.1 AAF14024.1 AAK62412.1 AAM97105.1 AAM98201.1 AAN15549.1 AEE74744.1 BAD94012.1 BAE98937.1 BAF02144.1 BAH20034.1 BAH20110.1 NP_187537.1	O24433 Q9SR37
ER β-glucosidase / ABA-β-glucosidase (BG1;Bgl1;Bglu18;AtBG1;At1g52400)	3.2.1.175 3.2.1.21	Arabidopsis thaliana	AAF22295.1 AAG21562.1 AAG51546.1 AAK63959.1 AAL08271.1 AAN18084.1 AAN31804.1 AAN60329.1 AEE32799.1 AEE32800.1 AEE32801.1 BAD94819.1 CAC19786.1 CBG20990.1 CBG21227.1 CBG21235.1 CBG21246.1 NP_175649.1	Q8H169 Q8H7A3 Q9FNZ3 Q9SE50
β-glucosidase (Sfr2;At3g06510)	3.2.1.21	Arabidopsis thaliana	AAF23823.1 AAG51335.1 AAL24371.1 AAL31914.1 AAM65250.1 AAN72111.1 AEE74404.1 AEE74405.1 CAD36512.1 CAD36513.1 NP_187303.1 NP_566285.1	Q8LAN2 Q8LGU5 Q8W578 Q93Y07 Q9C8Z2 Q9SDL5
β-mannosidase/β-glucosidase (Bglu44;At3g18080)	3.2.1.21 3.2.1.25	Arabidopsis thaliana	AAK25950.1 AAK64096.1 AAM61427.1 AEE76044.1 BAB02020.1 BAH19552.1 BAH19607.1 CBD33469.1 CBG20992.1 NP_188436.1	Q9LV33
ER β-glucosidase (Bglu22;At1g66280)	3.2.1.21	Arabidopsis thaliana	AAG12895.1 AAG52159.1 AAL67074.1 AEE34490.1 BAH56964.1 CAK32149.1 NP_176802.1	Q9C8Y9
myrosinase (TGG5;Bglu35;At1g51470)	3.2.1.147	Arabidopsis thaliana	AAG12767.1 AAG52628.1 ACO95140.1 AEE32671.1 NP_175558.1 NP_175558.3	Q3ECS3 Q9C8J9
myrosinase (M1;ArMY1)	3.2.1.147	Armoracia rusticana	AAV71147.1	Q5PXK2
β-glucosidase (Bgl1)	3.2.1.21	Aspergillus niger AMS11	AAF74209.2	Q9P456
β-glucosidase B3 (Bgl3;AO090003000497) (fragment)	3.2.1.21	Aspergillus oryzae RIB40	BAE57671.1
β-glucosidase B6 (AO090113000148)	3.2.1.21	Aspergillus oryzae RIB40	BAE63197.1
β-glucosidase I (avenacosidase;AsGlu1;As-Glu1;P60)	3.2.1.21	Avena sativa	CAA55196.1 CAW42867.1 CAW48570.1 CBC08395.1 CBD21363.1 CBG21201.1 S50756	Q38786
β-glucosidase II (AsGlu2;As-Glu2;P60b)	3.2.1.21	Avena sativa	AAD02839.1 CAW42887.1 CAW48590.1 CBC08415.1 CBD21383.1 CBG21243.1	Q9ZP27
β-glucosidase	3.2.1.21	Bombyx mori	AAP13852.1	Q86D78
myrosinase (Myr1)	3.2.1.147	Brassica juncea	CAA11412.1	Q9ZP01
myrosinase	3.2.1.147	Brassica juncea	AAG54074.1 CAW42883.1 CAW48586.1 CBC08411.1 CBD21379.1	Q9AWB5
zeatin β-glucosidase (Bgl)	3.2.1.21	Brassica napus	ACH11657.1 CAA57913.1 CAW42875.1 CAW48578.1 CBC08403.1 CBD21371.1	Q42618
myrosinase	3.2.1.147	Brassica napus	AAQ79764.1 ABQ42337.1 ADP24126.1 ADP24127.1 CAA42775.1 CAA55685.1 CAW42868.1 CAW42890.1 CAW48571.1 CAW48593.1 CBC08396.1 CBC08418.1 CBD21364.1 CBD21386.1 CBD31889.1 CBD31921.1	Q00326 Q39343
myrosinase 1	3.2.1.147	Brassica napus	CAA79989.2 CAW42886.1 CAW48589.1 CBC08414.1 CBD21382.1	Q9STD7
myrosinase 2	3.2.1.147	Brassica napus	CAA79990.1 CAW42876.1 CAW48579.1 CBC08404.1 CBD21372.1	Q42629
myrosinase (BMY1)	3.2.1.147	Brevicoryne brassicae	AAL25999.1 CBG21232.1	Q95X01	1WCG[A,B]
β-primeverosidase (Bgl;PD)	3.2.1.149	Camellia sinensis	AAR76593.1 AAR76595.1 AAS83105.1 ACH11663.1 ADV40931.1 ADV40931.2 BAC78656.1 CAK97604.1 CAK97604.2 CBG21219.1	Q7X9A9
β-glucosidase (BglB)	3.2.1.21	Candida wickerhamii CAPRIOTTI	AAC49036.1	Q12601
ipecoside β-glucosidase (IpeGlu1)	3.2.1.21	Carapichea ipecacuanha	BAH02544.1	B6ZKM3
myrosinase (β-thioglucoside glucohydrolase) (Tgg2)	3.2.1.147	Carica papaya	ACO95143.1	C9WCQ1
myrosinase (β-thioglucoside glucohydrolase) (Tgg1)	3.2.1.147	Carica papaya	ACO95142.1	C9WCQ0
strictosidine β-glucosidase	3.2.1.105	Catharanthus roseus	AAF28800.1 ABW77570.1 CAC09045.1	Q9M7N7
β-glucosidase	3.2.1.21	Cavia porcellus	AAB41058.1	P97265
furostanol glycoside 26-O-β-glucosidase	3.2.1.21	Cheilocostus speciosus	BAA11831.1 CBG21206.1	Q42707
non-cyanogenic β-glucosidase (Bglc-2)	3.2.1.21	Cicer arietinum	CAG14979.1 CAW42877.1 CAW48580.1 CBC08405.1 CBD21373.1	Q700B1
β-glucosidase (fragment)	3.2.1.21	Cicer arietinum	CAC08209.2	Q9FSY8
β-glucosidase	3.2.1.21	Coptotermes formosanus	ADB23476.1	D2X5V7
β-glucosidase (CjCel1A) (Cel1A)	3.2.1.21	Corbicula japonica	BAG71912.1 BAH14963.1	B5U9B3
dalcochinin β-glucosidase / β-fucosidase (Bglu1)	3.2.1.21 3.2.1.38	Dalbergia cochinchinensis	AAF04007.1	Q9SPK3
isoflavonoid 7-O-β-apiosyl-β-glucose β-glycosidase (DnBglu)	3.2.1.161 3.2.1.21 3.2.1.38	Dalbergia nigrescens KURZ.	AAV34606.1	Q5UB04
β-glucosidase (peptide fragment)	3.2.1.21	Daldinia eschscholzii
AA7GT / BgluL	2.4.1.-	Delphinium grandiflorum	BAJ33502.1	E3W9M3
acyl-glucose-dependent anthocyanin 5-O-glucosytransferase (BgluL;DcBgluL;AA5GT)	2.4.1.-	Dianthus caryophyllus	BAJ33501.1 BAL45928.1	E3W9M2
cardenolide 16'-O-glucohydrolase	3.2.1.21	Digitalis lanata	CAB38854.2 CAB38854.2	Q9ZPB6 Q9ZPB6
hydroxyisourate hydrolase (HIUHase)	3.5.2.17	Glycine max	AAL92115.1 CAW42880.1 CAW48583.1 CBC08408.1 CBD21376.1	Q8S3J3
isoflavone conjugate-specific β-glucosidase (GmICHG)	3.2.1.161 3.2.1.21	Glycine max	BAF34333.1	Q08IT7
lactase-phlorizin hydrolase (Lct)	3.2.1.108	Homo sapiens	AAA59504.1 AAP12677.1 AAX88924.1 ACM80708.1 ACM82337.1 CAA30801.1 NP_002290.1	P09848.1
lactase-phlorizin hydrolase (Lct)	3.2.1.62	Homo sapiens	AAA59504.1 AAP12677.1 AAQ79768.1 AAX88924.1 ACM80708.1 ACM82337.1 CAA30801.1 NP_002290.1	P09848.1
neutral β-glycosylceramidase / β-glucosidase (Gba3;CbgCBgl1;GluC;KLrP) (cytosolic)	3.2.1.21 3.2.1.62	Homo sapiens	AAG39217.1 AAH29362.1 AAI01830.1 AAI09378.1 AAL37305.1 BAB18741.1 BAD96683.1 BAF85435.1 BAG36006.1 BAH12772.1 CAC08178.1 NP_066024.1	A8K9N1 Q9H227.2	2E9L[A] 2E9M[A] 2JFE[X] 2ZOX[A]
β-glucosidase	3.2.1.21	Hordeum vulgare	AAA87339.1 CAW42869.1 CAW48572.1 CBC08397.1 CBD21365.1 CBG21202.1	Q40025
β-mannosidase / β-glucosidase (HvBII)	3.2.1.21 3.2.1.25 3.2.1.74	Hordeum vulgare subsp. vulgare	ACF07998.1	B5A496
β-glucosidase (Bgl4)	3.2.1.21	Humicola grisea var. thermoidea IFO9854	BAA74958.1	O93784
β-glucosidase 2 (Bgl2;BGLII) (Cel1A)	3.2.1.21	Hypocrea jecorina QM9414	BAA74959.1	O93785	3AHY[A,B,C,D]
β-1,4-glucosidase (Bgl2)	3.2.1.21	Hypocrea lixii T7	ABN50090.1 ABN50091.1	A3FPG4
β-glucosidase	3.2.1.21	Lasiodiplodia theobromae CBS 247.96	CAJ81226.1
male-specific β-glycosidase (Lmα-p72)	3.2.1.-	Leucophaea maderae	AAL40863.1	Q8WQL9
hydroxynitrile β-glucosidase D2 (LjBGD2)	3.2.1.21	Lotus japonicus	ACD65510.1	B2ZUU1
hydroxynitrile β-glucosidase D4 (LjBGD4)	3.2.1.21	Lotus japonicus	ACD65509.1	B2ZUU0
β-glucosidase	3.2.1.21	Malus x domestica	AEN94900.1
β-glucosidase (linamarase)	3.2.1.21	Manihot esculenta	AAB71381.1 CAW42866.1 CAW48569.1 CBC08394.1 CBD21362.1	O24524
β-glucosidase A	3.2.1.21	Manihot esculenta	CAA64442.1 CAW42870.1 CAW48573.1 CBC08398.1 CBD21366.1 CBG21203.1	Q40283
β-glucosidase (linamarase)	3.2.1.21	Manihot esculenta	AAB22162.1 CAW42872.1 CAW48575.1 CBC08400.1 CBD21368.1 CBG21204.1	Q41172
klotho (β-glucuronidase and sialidase activity) (KL)	3.2.1.18 3.2.1.31	Mus musculus	AAI38260.1 AAQ41830.1 BAA23381.1 BAA25308.1 CAC42188.1 NP_038851.1	O35082
klotho (β-glucuronidase and sialidase activity) (KL)	3.2.1.31	Mus musculus	AAI38260.1 AAQ41830.1 BAA23381.1 BAA25308.1 BAE22211.1 CAC42188.1 NP_038851.1	O35082
β-glucosidase (SgNtBG1;SgNtBG3)	3.2.1.21	Nasutitermes takasagoensis	BAI50018.1 BAI50020.1	D0VYR5
β-glucosidase (SgNtBG2)	3.2.1.21	Nasutitermes takasagoensis	BAI50019.1	D0VYR6
β-glucosidase (SgNtBG4)	3.2.1.21	Nasutitermes takasagoensis	BAI50021.1	D0VYR8
β-glucosidase (Bgl595;NfBGL595;NFIA_060550)	3.2.1.21	Neosartorya fischeri NRRL 181
aryl β-glucosidase (Bgl1;NfBGL1;BGL;NFIA_064710)	3.2.1.21	Neosartorya fischeri NRRL 181
β-glucosidase (Bgl;NkBgl;NkBG;G1NkBG)	3.2.1.21	Neotermes koshunensis	BAB91145.1	Q8T0W7	3AHZ[A] 3AI0[A]
β-glucosidase (Gh1-1;NCU00130)	3.2.1.21	Neurospora crassa OR74A	EAA26947.1 XP_322216.1
β-glucosidase (Bgl1)	3.2.1.21	Orpinomyces sp. PC-2	AAD45834.1	Q9UUQ3
lactase phlorizin hydrolase (LCT;LPH)	3.2.1.108 3.2.1.62	Oryctolagus cuniculus	CAA30802.1	P09849.1
lactase phlorizin hydrolase (LCT;LPH)	3.2.1.108 3.2.1.62	Oryctolagus cuniculus	AAQ79765.1	P09849.1
lactase-phlorizin hydrolase	3.2.1.108 3.2.1.62	Oryctolagus cuniculus	CAA81690.1	Q29518
lactase-phlorizin hydrolase	3.2.1.108 3.2.1.62	Oryctolagus cuniculus	CAA81690.1	Q29518
β-glucosidase isozyme 2 (Os09g0491100;Os9bglu30)	3.2.1.21 3.2.1.38	Oryza sativa Japonica Group	AAL14713.1 BAF25453.1	Q0J0N4
β-glucosidase (Os03g0212800;Os3bglu6)	3.2.1.21	Oryza sativa Japonica Group	AAN01354.1 BAF11272.1 BAG99682.1	Q8L7J2	3GNO[A] 3GNP[A] 3GNR[A]
β-mannosidase / β-glucosidase (Os7bglu26;Os07g0656200)	3.2.1.21 3.2.1.25 3.2.1.74	Oryza sativa Japonica Group	BAC16123.1 BAC20802.1 BAF22416.1
tuberonic acid glucoside-hydrolyzing β-glucosidase (OsTAGG1;Os4bglu13;Os04g0474900)	3.2.1.21	Oryza sativa Japonica Group	BAF14985.1 CAE05485.2 CAE05491.1	A3AZS2 Q7XKV2
β-glucosidase (Os04g0474800;Os4bglu12) (β-1,3-1,4-oligosaccharide and tuberonic acid glucoside-hydrolyzing)	3.2.1.21	Oryza sativa Japonica Group	BAF14984.1 BAG94781.1 CAE05483.2 CAE05489.1	Q7XKV4	3PTK[A,B] 3PTM[A,B] 3PTQ[A,B]
β-glucosidase (Os3bglu8;Os03g0703100)	3.2.1.21 3.2.1.74	Oryza sativa Japonica Group	AAS07251.1 AAT85323.1 AAX95520.1 BAF12928.1 BAH00173.1 CBD28470.1	Q53RI4
β-glucosidase (Bglu1;Os03g0703000;Os3bglu7)	3.2.1.21	Oryza sativa Japonica Group	AAA84906.2 AAA84906.3 AAS07254.1 AAS07255.1 AAT85322.1 AAX95519.1 BAF12927.1 BAG94472.1 CBD28462.1 CBD28469.1	Q42975 Q75I93	2RGL[A,B] 2RGM[A,B] 3AHT[A,B] 3AHV[A,B] 3F4V[A,B] 3F5I[A,B] 3F5J[A,B] 3F5K[A,B] 3F5L[A,B]
indican β-glucosidase	3.2.1.21	Persicaria tinctoria	BAA78708.1 CBG21240.1	Q9XJ67
β-glucosidase (Bgl1A)	3.2.1.21	Phanerochaete chrysosporium K-3	BAE87008.1	Q25BW5	2E3Z[A,B] 2E40[A,B]
β-glucosidase (Bgl1B)	3.2.1.21	Phanerochaete chrysosporium K-3	BAE87009.1	Q25BW4
monolignol β-glucosidase	3.2.1.21	Pinus contorta	AAC69619.1 AAE33518.1	Q9ZT64
β-glucosidase (Bgl1A) (Bgl1A=Cel1A)	3.2.1.21	Piromyces sp. E2	CAC34952.1	Q9C122
β-glucosidase	3.2.1.21	Prunus avium	AAA91166.1 ACH11661.1	Q43014
prunasin hydrolase isoform PH B (PH-L3)	3.2.1.118	Prunus serotina	AAL06338.1 AAL39079.1 CAW42881.1 CAW48584.1 CBC08409.1 CBD21377.1	Q8W1W7 Q945N9
prunasin hydrolase isoform PH C (PH-L4)	3.2.1.118	Prunus serotina	AAL07434.1 AAL35324.1	Q8W594 Q945I4
prunasin hydrolase isoform PH-L1	3.2.1.118	Prunus serotina	AAF34651.2	Q9M5X4
prunasin hydrolase isoform PH A (PH-L2) (fragment)	3.2.1.118	Prunus serotina	AAL07435.1	Q945I3
prunasin hydrolase isoform PHA	3.2.1.118	Prunus serotina	AAF34650.1	Q9M5X5
β-glucosidase (PH-S1)	3.2.1.21	Prunus serotina	AAA93032.1 AAW06330.1	Q43073
amygdalin β-glucosidase I (AH I;AH1)	3.2.1.117	Prunus serotina	AAA93234.2 AAL07489.1 CAW42871.1 CAW48574.1 CBC08399.1 CBD21367.1	P29259.1 P29260.1 P29261.1 P29262.1 Q40984 Q945G7
β-glucosidase G2 (peptide fragments)	3.2.1.21	Pythium irregulare
β-glucosidase G3 (peptide fragments)	3.2.1.21	Pythium irregulare
myrosinase (RMB2)	3.2.1.147	Raphanus sativus	BAB17227.1	Q9FXR0
myrosinase (RMB1)	3.2.1.147	Raphanus sativus	BAB17226.1	Q9FXR1
strictosidine β-glucosidase (Sgr1;SG)	3.2.1.105	Rauvolfia serpentina	CAC83098.1 CBG21226.1	Q8GU20	2JF6[A,B] 2JF7[A,B]
raucaffricine β-glucosidase (RG)	3.2.1.125	Rauvolfia serpentina	AAF03675.1	Q9SPP9	3U57[A,B] 3U5U[A,B] 3U5Y[A,B]
β-glucosidase (BGluc-1;RfBGluc-1)	3.2.1.21 3.2.1.37 3.2.1.38	Reticulitermes flavipes	ADK12988.1	D9ILU1
β-glucosidase (ScGlu) (plastidic)	3.2.1.21	Secale cereale	AAG00614.1 CAW42885.1 CAW48588.1 CBC08413.1 CBD21381.1 CBG21237.1	Q9FYS3	3AIU[A] 3AIV[A] 3AIW[A]
myrosinase	3.2.1.147	Sinapis alba	AAQ79766.1 CAA42534.1	P29092.1
myrosinase (fragment)	3.2.1.147	Sinapis alba	CAA42533.1	P29736
myrosinase (fragment)	3.2.1.147	Sinapis alba	CAA42536.1	P29737.1
myrosinase (fragment)	3.2.1.147	Sinapis alba	CAA42535.1	P29738.1
myrosinase	3.2.1.147	Sinapis alba		P29736 Q7SIB0	1DWA[M] 1DWF[M] 1DWG[M] 1DWH[M] 1DWI[M] 1DWJ[M] 1E4M[M] 1E6Q[M] 1E6S[M] 1E6X[M] 1E70[M] 1E71[M] 1E72[M] 1E73[M] 1MYR[A] 1W9B[M] 1W9D[M] 2MYR 2WXD[M]
β-mannosidase (LeMside 2)	3.2.1.25	Solanum lycopersicum	AAL37714.1 AAL37719.1 CBD28478.1	Q8VWL8
cyanogenic β-glucosidase (dhurrinase-2)	3.2.1.21	Sorghum bicolor	AAK49119.1 CAW42882.1 CAW48585.1 CBC08410.1 CBD21378.1 CBG21230.1	Q93XR2
cyanogenic β-glucosidase (dhurrinase 1)	3.2.1.21	Sorghum bicolor P721N	AAC49177.1 CAW42873.1 CAW48576.1 CBC08401.1 CBD21369.1 CBG21205.1	Q41290	1V02[A,B,C,D,F] 1V03[A]
β-glucosidase (β-gly;SfÎ²gly)	3.2.1.- 3.2.1.21 3.2.1.23 3.2.1.38	Spodoptera frugiperda	AAC06038.1	O61594
β-galactosidase/β-glucosidase (BglA)	3.2.1.21 3.2.1.23	Sporobolomyces singularis ATCC 24193	BAD95570.1	Q564N5
β-glucosidase (peptide fragments)	3.2.1.21	Stereum hirsutum SKU512
β-glucosidase 1 (Bg1;BglI;BGIV)	3.2.1.21	Talaromyces emersonii CBS 814.70	AAL34084.2 AAL89551.1	Q8X214
β-glucosidase	3.2.1.21	Tenebrio molitor	AAG26008.1	Q9GSE6
β-glucosidase 1 (non-cyanogenic)	3.2.1.21	Trifolium repens	AAQ79767.1 CAA40058.1 CAW42889.1 CAW48592.1 CBC08417.1 CBD21385.1	P26204.1
β-glucosidase 2 (cyanogenic)	3.2.1.21	Trifolium repens	ABV54716.1 ABV54717.1 ABV54718.1 ABV54719.1 ABV54720.1 ABV54721.1 ABV54722.1 ABV54723.1 ABV54724.1 ABV54725.1 ABV54726.1 ABV54727.1 ABV54728.1 ABV54729.1 ABV54730.1 ABV54731.1 ABV54732.1 ABV54733.1 ABV54734.1 ABV54735.1 ABV54736.1 ABV54737.1 ABV54738.1 ABV54739.1 ABV54740.1 ABV54741.1 ABV54742.1 ABV54743.1 ABV54744.1 ABV54745.1 ABV54746.1 ABV54747.1 ABV54748.1 ABV54749.1 CAA40057.1	A8C6G0 P26205.1	1CBG[A]
β-glucosidase (Glu1B;TaGlu1b)	3.2.1.21	Triticum aestivum	BAE92259.1	Q1XH05	2DGA[A] 3AIQ[A] 3AIR[A] 3AIS[A]
furcatin hydrolase (FH)	3.2.1.161	Viburnum furcatum	BAD14925.1	Q75W17
vicianin hydrolase β-glucosidase (VH)	3.2.1.21	Vicia sativa subsp. nigra	ABD03937.1	A2SY66
β-glucosidase (fragment)	3.2.1.21	Vitis vinifera	AAK72100.1	Q93X78
zeatin β-glucosidase (Glu1;p60.1;Zm-p60.1)	3.2.1.21	Zea mays	CAA52293.1 CAM55487.1 CAM55549.1 CAW42858.1 CAW42907.1 CAW48561.1 CAW48562.1 CAW48610.1 CBC08386.1 CBC08387.1 CBD21354.1 CBD21355.1	P49235 Q53WW9	1HXJ[A,B]
β-glucosidase 2 (Glu2)	3.2.1.21	Zea mays	AAD09850.1 AEM17055.1 CAM55515.1 CAM55640.1 CAW42861.1 CAW42874.1 CAW48564.1 CAW48577.1 CBC08389.1 CBC08402.1 CBD21357.1 CBD21370.1	Q41761
β-glucosidase 1 (Glu1)	3.2.1.21	Zea mays	AAA65946.1 AAB03266.1 AAD10503.1 ACF82863.1 ACG32200.1 CAM55450.1 CAM55655.1 CAW42888.1 CAW48591.1 CBC08416.1 CBD21384.1	P49235 Q53WW9	1E1E[A,B] 1E1F[A,B] 1E4L[A,B] 1E4N[A,B] 1E55[A,B] 1E56[A,B] 1H49[A,B] 1V08[A,B]

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.