Known Activities	β-glucosidase (EC 3.2.1.21); β-galactosidase (EC 3.2.1.23); β-mannosidase (EC 3.2.1.25); β-glucuronidase (EC 3.2.1.31); β-D-fucosidase (EC 3.2.1.38); phlorizin hydrolase (EC 3.2.1.62); exo-β-1,4-glucanase (EC 3.2.1.74); 6-phospho-β-galactosidase (EC 3.2.1.85); 6-phospho-β-glucosidase (EC 3.2.1.86); strictosidine β-glucosidase (EC 3.2.1.105); lactase (EC 3.2.1.108); amygdalin β-glucosidase (EC 3.2.1.117); prunasin β-glucosidase (EC 3.2.1.118); raucaffricine β-glucosidase (EC 3.2.1.125); thioglucosidase (EC 3.2.1.147); β-primeverosidase (EC 3.2.1.149); isoflavonoid 7-O-β-apiosyl-β-glucosidase (EC 3.2.1.161); hydroxyisourate hydrolase (EC 3.-.-.-); β-glycosidase (EC 3.2.1.-)
Mechanism	Retaining
Clan	GH-A
3D Structure Status	( β / α ) 8
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental); absent in plant myrosinases
External resources	CAZypedia; HOMSTRAD; InterPro; PFAM; PRINTS; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (3825); Uniprot accession (1211); PDB accession (117); 3D entries (29); cryst (2)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
alkyl β-glucosidase (membrane-bound) (BGPh;PH0366)	3.2.1.21	Pyrococcus horikoshii OT3	ABC06393.1 BAA29440.1 NP_142340.1	O58104	1VFF[A]
β-glycosidase S (LacS;Ss-β-gly;SSO3019)	3.2.1.- 3.2.1.21 3.2.1.23	Sulfolobus solfataricus P2	AAA72843.1 AAE29896.1 AAK43121.1 ABC06397.1 ACW34039.1 CAC24042.1 CAJ18790.1 NP_344331.1	P22498.2	1GOW[A,B] 1UWI[A,B,C,D] 1UWQ[A,B] 1UWR[A,B] 1UWS[A,B] 1UWT[A,B] 1UWU[A,B] 2CEQ[A,B] 2CER[A,B]
β-glycosidase (Tα-β-gly)	3.2.1.-	Thermosphaera aggregans M11TL	AAD43138.1 AAM56782.1	Q9YGA8	1QVB[A,B]
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
β-glucosidase (BglA)	3.2.1.21	Bacillus circulans subsp. alkalophilus	AAA22266.1 AAQ79761.1 CBG21249.1	Q03506	1QOX[A]
β-glucosidase A (BglA)	3.2.1.21	Clostridium cellulovorans	AAQ00997.1 CBG21209.1	Q53EH2	3AHX[A,B,C,D]
6-P-β-galactosidase (LacG)	3.2.1.85	Lactococcus lactis Z268	AAA25173.1 AAA25183.1 AAA26949.1 AAQ79752.1 CBG21261.1	P11546.2	1PBG[A,B] 2PBG[A] 3PBG[A,B] 4PBG[A,B]
β-glucosidase B (BglB)	3.2.1.21	Paenibacillus polymyxa	AAA22264.1 AAQ79762.1 CBG21256.1	P22505.1	2JIE[A] 2O9P[A] 2O9R[A] 2O9T[A] 2Z1S[A]
β-glucosidase A (BglA)	3.2.1.21	Paenibacillus polymyxa	AAA22263.1 AAQ79760.1 CBG21252.1	P22073.1	1BGA[A,B,C,D] 1BGG[A,B,C,D] 1E4I[A] 1TR1[A,B,C,D] 1UYQ[A]
β-glucosidase (Bgl3)	3.2.1.21	Streptomyces sp. QM-B814	CAA82733.1 CBG21211.1	Q59976	1GNX[A,B] 1GON[A,B]
β-glucosidase A (BglA)	3.2.1.21	Thermotoga maritima MSB8	CAA52276.1 CBG21253.1	Q08638	1OD0[A,B] 1OIF[A,B] 1OIM[A,B] 1OIN[A,B] 1UZ1[A,B] 1W3J[A,B] 2CBU[A,B] 2CBV[A,B] 2CES[A,B] 2CET[A,B] 2J75[A,B] 2J77[A,B] 2J78[A,B] 2J79[A,B] 2J7B[A,B] 2J7C[A,B] 2J7D[A,B] 2J7E[A,B] 2J7F[A,B] 2J7G[A,B] 2J7H[A,B] 2JAL[A,B] 2VRJ[A,B] 2WBG[A,B,C,D] 2WC3[A,B,C,D] 2WC4[A,B,C,D]
β-glycosidase (Gly)	3.2.1.21	Thermus nonproteolyticus HG102	AAF36392.1	Q9L794	1NP2[A,B]
β-glycosidase (TTHB087)		Thermus thermophilus HB8	AAN05439.1 BAD71883.1 CBG21166.1	Q53W75 Q8GEB3	1UG6[A]
β-glycosidase / β-glucosidase (BglA) (Bgl1A)	3.2.1.- 3.2.1.21	uncultured bacterium	ABI18350.1	Q0GMU3	3CMJ[A] 3FIY[A] 3FIZ[A] 3FJ0[A]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D
myrosinase (BMY1)	3.2.1.147	Brevicoryne brassicae	AAL25999.1 CBG21232.1	Q95X01	1WCG[A,B]
neutral β-glycosylceramidase / β-glucosidase (Gba3;CbgCBgl1;GluC;KLrP) (cytosolic)	3.2.1.21 3.2.1.62	Homo sapiens	AAG39217.1 AAH29362.1 AAI01830.1 AAI09378.1 AAL37305.1 BAB18741.1 BAD96683.1 BAF85435.1 BAG36006.1 BAH12772.1 CAC08178.1 NP_066024.1	A8K9N1 Q9H227.2	2E9L[A] 2E9M[A] 2JFE[X] 2ZOX[A]
β-glucosidase 2 (Bgl2;BGLII) (Cel1A)	3.2.1.21	Hypocrea jecorina QM9414	BAA74959.1	O93785	3AHY[A,B,C,D]
β-glucosidase (Bgl;NkBgl;NkBG)	3.2.1.21	Neotermes koshunensis	BAB91145.1	Q8T0W7	3AHZ[A] 3AI0[A]
β-1,3-1,4-oligoglucosidase/transglucosidase / β-glucosidase (Os04g0474800;Os4bglu12)	2.4.1.- 3.2.1.- 3.2.1.21	Oryza sativa Japonica Group	BAF14984.1 BAG94781.1 CAE05483.2 CAE05489.1	Q7XKV4	cryst
β-glucosidase (Os03g0212800;Os3bglu6)	3.2.1.21	Oryza sativa Japonica Group	AAN01354.1 BAF11272.1 BAG99682.1	Q8L7J2	3GNO[A] 3GNP[A] 3GNR[A]
β-glucosidase (Bglu1;Os03g0703000;Os3bglu7)	3.2.1.21	Oryza sativa Japonica Group	AAA84906.2 AAA84906.3 AAS07254.1 AAS07255.1 AAT85322.1 AAX95519.1 BAF12927.1 BAG94472.1 CBD28462.1 CBD28469.1	Q42975	2RGL[A,B] 2RGM[A,B] 3AHT[A,B] 3AHV[A,B] 3F4V[A,B] 3F5I[A,B] 3F5J[A,B] 3F5K[A,B] 3F5L[A,B]
β-glucosidase (Bgl1A)	3.2.1.21	Phanerochaete chrysosporium K-3	BAE87008.1	Q25BW5	2E3Z[A,B] 2E40[A,B]
raucaffricine β-glucosidase	3.2.1.125	Rauvolfia serpentina	AAF03675.1	Q9SPP9	cryst
strictosidine β-glucosidase (Sgr1)	3.2.1.105	Rauvolfia serpentina	CAC83098.1 CBG21226.1	Q8GU20	2JF6[A,B] 2JF7[A,B]
myrosinase	3.2.1.147	Sinapis alba		P29736 Q7SIB0	1DWA[M] 1DWF[M] 1DWG[M] 1DWH[M] 1DWI[M] 1DWJ[M] 1E4M[M] 1E6Q[M] 1E6S[M] 1E6X[M] 1E70[M] 1E71[M] 1E72[M] 1E73[M] 1MYR[A] 1W9B[M] 1W9D[M] 2MYR 2WXD[M]
cyanogenic β-glucosidase (dhurrinase 1)	3.2.1.21	Sorghum bicolor P721N	AAC49177.1 CAW42873.1 CAW48576.1 CBC08401.1 CBD21369.1 CBG21205.1	Q41290	1V02[A,B,C,D,F] 1V03[A]
β-glucosidase 2 (cyanogenic)	3.2.1.21	Trifolium repens	ABV54716.1 ABV54717.1 ABV54718.1 ABV54719.1 ABV54720.1 ABV54721.1 ABV54722.1 ABV54723.1 ABV54724.1 ABV54725.1 ABV54726.1 ABV54727.1 ABV54728.1 ABV54729.1 ABV54730.1 ABV54731.1 ABV54732.1 ABV54733.1 ABV54734.1 ABV54735.1 ABV54736.1 ABV54737.1 ABV54738.1 ABV54739.1 ABV54740.1 ABV54741.1 ABV54742.1 ABV54743.1 ABV54744.1 ABV54745.1 ABV54746.1 ABV54747.1 ABV54748.1 ABV54749.1 CAA40057.1	A8C6G0 P26205.1	1CBG[A]
β-glucosidase (TaGlu1b)	3.2.1.21	Triticum aestivum	BAE92259.1	Q1XH05	2DGA[A]
zeatin β-glucosidase (p60.1;Zm-p60.1)	3.2.1.21	Zea mays	CAA52293.1 CAM55487.1 CAM55549.1 CAW42858.1 CAW42907.1 CAW48561.1 CAW48562.1 CAW48610.1 CBC08386.1 CBC08387.1 CBD21354.1 CBD21355.1	P49235 Q53WW9	1HXJ[A,B]
β-glucosidase 1 (Glu1)	3.2.1.21	Zea mays	AAA65946.1 AAB03266.1 AAD10503.1 ACF82863.1 ACG32200.1 CAM55450.1 CAM55655.1 CAW42888.1 CAW48591.1 CBC08416.1 CBD21384.1	P49235 Q53WW9	1E1E[A,B] 1E1F[A,B] 1E4L[A,B] 1E4N[A,B] 1E55[A,B] 1E56[A,B] 1H49[A,B] 1V08[A,B]