Activities in Sub Family	[retaining] amylosucrase (EC 2.4.1.4); [retaining] sucrose α-glucosidase (EC 3.2.1.48);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (29); Uniprot accession (12); PDB accession (26); 3D entries (7); cryst (0)

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
JNO48_03585		Clostridiales bacterium XBB3002	QTE69671.1			4
psyc5s11_26970		Clostridium gelidum C5S11	BCZ46630.1			4
CE91St63_23780		[Clostridium] hylemonae CE91-St63	BDF05316.1			4
LAJLEIBI_02323 (AmS)		[Clostridium] hylemonae DSM 15053	QEK18306.1			4
C820_002157 (Ams_1)		Clostridium sp. MD294 ASF356	USF30714.1			4
C820_002792 (Ams_2)		Clostridium sp. MD294 ASF356	USF31346.1			4
os1_40150 (AmS)		Comamonadaceae bacterium OS-1	BDT69823.1			4
NQ556_03850		Coprococcus comes ATCC 27758	UWP14846.1			4
V3C08_04480		Coprococcus comes DFI.3.84	WVP81100.1			4
I6K69_13860		Coprococcus comes FDAARGOS_1339	QRT49301.1			4
NQ536_04370		Coprococcus eutactus ATCC 27759	UWP17885.1			4
CCU_01540		Coprococcus sp. ART55/1	CBK82085.1			4
SH580_14590 (fragment)		Coraliomargarita sp. J2-16	WPJ94660.1			4
SH580_14595 (fragment)		Coraliomargarita sp. J2-16	WPJ94661.1			4
SCMU_08190		Corynebacterium cyclohexanicum ATCC 51369	BCT74977.1			4
HYG79_09200		Costertonia aggregata KCCM 42265	QLG45515.1			4
Mal65_31250 (AmS)		Crateriforma conspicua Mal65	QDV63978.1			4
PAZ_c09350		Cutibacterium acnes 266	AEE72114.1			4
TIB1ST10_04635 (fragment)		Cutibacterium acnes 6609	AEH29224.1			4
CPA42_04810		Cutibacterium acnes ATCC 6919	AVT26409.1			4
F6X01_04835		Cutibacterium acnes ATCC 6919	QEW95563.1			4
PAC1_04750		Cutibacterium acnes C1	AFU40712.1			4
OYC59_001395		Cutibacterium acnes CBS-BPNBT19153	WGH33492.1			4
OYC57_001398		Cutibacterium acnes CBS-BPNBT19223	WGH37979.1			4
OYC58_000920		Cutibacterium acnes CBS-BPNBT19227	WGH37302.1			4
OYC54_000960		Cutibacterium acnes CBS-BPNBT19269	WGH46332.1			4
cact_04830		Cutibacterium acnes DSM 1897	QAZ48485.1			4
EGY06_02910		Cutibacterium acnes FDAARGOS_503	AYZ24899.1			4
EGX38_09155		Cutibacterium acnes FDAARGOS_577	AYW80648.1			4
TIA1EST1_04420		Cutibacterium acnes hdn-1	AID35699.1			4
PAGK_1251		Cutibacterium acnes HL096PA1	AGJ80085.1			4
ALW29_04475		Cutibacterium acnes PA_12_1_L1	ALT41948.1			4
ALW27_04485		Cutibacterium acnes PA_12_1_R1	ALT39711.1			4
ALW33_04635		Cutibacterium acnes PA_15_1_R1	ALT44236.1			4
ALW23_04630		Cutibacterium acnes PA_15_2_L1	ALT37435.1			4
ALW22_04530		Cutibacterium acnes PA_30_2_L1	ALT32866.1			4
HMPREF0675_3957		Cutibacterium acnes SK137	ADD99646.1			4
CacPP4_08650		Cutibacterium acnes subsp. acnes NBRC 107605	BBK84250.1			4
TIIST44_10160 (fragment)		Cutibacterium acnes subsp. defendens ATCC 11828	AER06487.1			4
CASZ1_08590		Cutibacterium acnes SZ1	BCB10730.1			4
CASZ2_08610		Cutibacterium acnes SZ2	BCB12957.1			4
TPCU389_03870		Cutibacterium acnes TP-CU389	BBJ75862.1			4
TPCU411_08330		Cutibacterium acnes TP-CU411	BDE67228.1			4
TPCU426_08610		Cutibacterium acnes TP-CU426	BDQ42064.1			4
TIA2EST22_04475		Cutibacterium acnes TypeIA2 P.acn17	AEW81215.1			4
TIA2EST36_04445		Cutibacterium acnes TypeIA2 P.acn31	AEW83479.1			4
TIA2EST2_04395		Cutibacterium acnes TypeIA2 P.acn33	AEW78972.1			4
P2W57_02330		Cutibacterium acnes YM-1	WET46316.1			4
PALO_06565		Cutibacterium avidum 44067	AGJ77921.1			4
BFS79_02590		Cutibacterium avidum DPC 6544	AOG27602.1			4
JMX58_04055		Cutibacterium avidum KCTC 5339	QRH10757.1			4
JMX17_10410		Cutibacterium avidum KGMB02810	QQY12471.1			4
JMX53_09165		Cutibacterium avidum KGMB09337	QQY14458.1			4
TPCV302_14550		Cutibacterium avidum TP-CV302	BDY02063.1			4
TPCV4_08810		Cutibacterium avidum TP-CV4	BCQ02437.1			4
TPCV14_08690		Cutibacterium avidum TPCV-14	BCQ04825.1			4
KB1_14220		Cutibacterium modestum KB17-24694	BCY25432.1			4
Cyagr_0977		Cyanobium gracile PCC 6307	AFY28159.1			4
OGCDGJMD_01548 (AmS)		Cyanobium usitatum str. Tous	CAK6694013.1			4
CYPRO_2346		Cyclonatronum proteinivorum Omega	AXJ01588.1			4
AUC44_02855		Deinococcus actinosclerus BM2	ALW87966.1			4
DM785_03325		Deinococcus actinosclerus SJTR	AWT34695.1			4
DAETH_23290		Deinococcus aetherius ST0316	BDP42360.1			4
M8445_07725		Deinococcus aquaticus PB-314	WDA57267.1			4
Deide_05900		Deinococcus deserti VCD115	ACO45430.1			4
amylosucrase (DgAS; Dgeo_0572)	2.4.1.4	Deinococcus geothermalis DSM 11300	ABF44874.1 YP_604043.1	Q1J0W0	3UCQ[A] 3UER[A]	4
DGo_CA0887		Deinococcus gobiensis I-0	AFD24814.1			4
DEGR_05120		Deinococcus grandis ATCC 43672	BBN93779.1			4
DKM44_11020		Deinococcus irradiatisoli 17bor-2	AWN23694.1			4
Deima_2728		Deinococcus maricopensis DSM 21211	ADV68358.1			4
E5F05_10410		Deinococcus metallilatus MA1002	QBY08313.1			4
Deipe_2892		Deinococcus peraridilitoris DSM 19664	AFZ68353.1			4
EHF33_05715		Deinococcus psychrotolerans S14-83T	AZI43805.1			4
SU48_12345		Deinococcus puniceus DY1	ANE44420.1			4
E5E91_04845		Deinococcus radiodurans ATCC 13939	UDL00079.1			4
HAV35_09200		Deinococcus radiodurans BND-54	QIP32253.1			4
HAV23_07595		Deinococcus radiodurans BNK-50	QIP29042.1			4
DRO_0925		Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	UID69925.1			4
amylosucrase (AS;DrAS;DR0933)	2.4.1.4	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	AAF10510.1 ANC71873.1 NP_294657.1	Q9RVT9	4AYS[A]	4
DXG80_00755		Deinococcus radiodurans R1 dM1	QEM70429.1			4
amylosucrase (DRpAS)	2.4.1.4	Deinococcus radiopugnans ATCC 19172	MBB6016233.1 QCT05769.1			4
HLB42_04465		Deinococcus radiopugnans Mariana-D7000	QLG12367.1			4
N0D28_13325		Deinococcus rubellus Ant6	UWX63699.1			4
SY84_11600		Deinococcus soli (ex Cha et al. 2016) N5	AKH17570.1			4
U2P90_14930		Deinococcus sp. AB2017081	WQE94688.1			4
DAAJ005_12080		Deinococcus sp. AJ005	QFP78373.1			4
MF271_16050		Deinococcus sp. KNUC1210	ULH15415.1			4
M1R55_08800		Deinococcus sp. QL22	UQN05010.1			4
WDJ50_05275		Deinococcus sp. VB142	WYF45538.1			4
QR90_04310		Deinococcus swuensis DY59	AIZ46461.1			4
LAJ19_01755		Deinococcus taeanensis TS293	UBV42975.1			4
amylosucrase (DwAS)	2.4.1.4	Deinococcus wulumuqiensis	WP_017868998.1			4
DVJ83_06585		Deinococcus wulumuqiensis NEB 479	AXH00069.1			4
G6R31_07410		Deinococcus wulumuqiensis R12	QII20608.1			4
DAD186_03850		Dermabacter vaginalis AD1-86	ANP26942.1			4
FOB48_09455		Dermabacter vaginalis FDAARGOS_640	QEU12788.1			4
FV141_13065		Dermacoccus abyssi HZAU 226	QEH94342.1			4
H7F30_04375		Dermacoccus sp. PAMC28757	QNK53544.1			4
AAM4_0251		Desulfitobacterium hafniense	CED90146.1			4
EYB58_08170		Desulfobacter hydrogenophilus AcRS1	QBH12892.1			4
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