Glycoside Hydrolase Family 13 / Subf 4

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (428); Uniprot accession (71); PDB accession (24); 3D entries (6); cryst (0)
Bacteria
Protein Name EC#OrganismGenBank UniprotPDB/3DSubf
 amylosucrase (Amas) 2.4.1.4 Alteromonas macleodii KCTC 2957 BAG82876.1 B6F2H1   4
 amylosucrase (DgAS;Dgeo_0572) 2.4.1.4 Deinococcus geothermalis DSM 11300 ABF44874.1
YP_604043.1
Q1J0W0 3UCQ[A]
3UER[A]
4
 amylosucrase (AS;DrAS;DR0933) 2.4.1.4 Deinococcus radiodurans R1 AAF10510.1
ANC71873.1
NP_294657.1
Q9RVT9 4AYS[A] 4
 amylosucrase (MFAS;Mfla_2611) 2.4.1.4 Methylobacillus flagellatus KT ABE50875.1 Q1GY12   4
 amylosucrase (Ams;NpAS) 2.4.1.4 Neisseria polysaccharea ATCC 43768 AAT15258.1
AAW13710.1
ACK26675.1
CAA03058.1
CAA03083.1
CAA09772.1
E2PEX2
Q9ZEU2
1G5A[A]
1JG9[A]
1JGI[A]
1MVY[A]
1MW0[A]
1MW1[A]
1MW2[A]
1MW3[A]
1S46[A]
1ZS2[A]
3UEQ[A]
4FLO[A]
4FLQ[A]
4FLR[A]
4FLS[A]
4
 amylosucrase (AcAS;Achl_3605) 2.4.1.4 Pseudarthrobacter chlorophenolicus A6 ACL41561.1 B8H6N5   4
 amylosucrase (AmsA;1986-orf;AMS;SYNPCC7002_A0885) 2.4.1.4 Synechococcus sp. PCC 7002 ACA98889.1
CDL67995.1
B1XIU7   4
 sucrose hydrolase (XAC3490) 3.2.1.- Xanthomonas axonopodis pv. citri str. 306 AAM38333.1
NP_643797.1
Q8PGX2   4
 sucrose hydrolase (Suh) 3.2.1.- Xanthomonas axonopodis pv. glycines 8RA AAQ93678.1 Q6UVM5 3CZE[A]
3CZG[A]
3CZK[A]
3CZL[A]
4
 sucrose hydrolase (SuxB;XCC3359) 3.2.1.- Xanthomonas campestris pv. campestris str. ATCC 33913 AAM42629.1
NP_638705.1
Q8P5I2 2WPG[A] 4
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