Activities in Sub Family	[retaining] amylosucrase (EC 2.4.1.4);[retaining] sucrose α-glucosidase (EC 3.2.1.48)
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1473); Uniprot accession (12); PDB accession (26); 3D entries (7); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
α-amylase family protein		Calidithermus timidus	WP_018466847.1		7ESH[A,B,C,D] 2.29	4
amylosucrase (DgAS; Dgeo_0572)	2.4.1.4	Deinococcus geothermalis DSM 11300	ABF44874.1 YP_604043.1	Q1J0W0	3UCQ[A] 1.97 3UER[A] α-D-Glcp-(1-3)-α-D-Fruf 2.10	4
amylosucrase (AS;DrAS;DR0933)	2.4.1.4	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	AAF10510.1 ANC71873.1 NP_294657.1	Q9RVT9	4AYS[A] 3.15	4
amylosucrase (Ams)		Neisseria polysaccharea 85322	AAM51153.1		5N6V[A] α-D-Glcp α-D-Glcp-(1-2)-β-D-Fruf β-D-Fruf 1.60 5N7J[A] α-D-Glcp-(1-2)-β-D-Fruf α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.00	4
amylosucrase (Ams; NpAS)	2.4.1.4	Neisseria polysaccharea ATCC 43768	AAT15258.1 AAW13710.1 ACK26675.1 CAA03058.1 CAA03083.1 CAA09772.1	E2PEX2 Q9ZEU2	1G5A[A] 1.40 1JG9[A] β-D-Glcp 1.66 1JGI[A] α-D-Glcp-(1-2)-β-D-Fruf 2.00 1MVY[A] α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.00 1MW0[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.01 1MW1[A] α-D-Glcp-(1-2)-β-D-Fruf 2.10 1MW2[A] α-D-Glcp-(1-2)-β-D-Fruf α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.10 1MW3[A] α-D-Glcp-(1-2)-β-D-Fruf 2.00 1S46[A] β-D-Glcp-(1-4)-ASP 2.20 1ZS2[A] α-D-Glcp-(1-2)-β-D-Fruf α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp 2.16 3UEQ[A] α-D-Glcp-(1-1)-<non_carb> α-D-Glcp-(1-3)-β-D-Fruf 1.85 4FLO[A] 2.20 4FLQ[A] 2.50 4FLR[A] 2.40 4FLS[A] α-D-Glcp-(1-2)-β-D-Fruf 2.30	4
sucrose hydrolase (SUH; XaSH)	3.2.1.48	Xanthomonas axonopodis pv. glycines 8RA	AAQ93678.1	Q6UVM5	3CZE[A] 1.90 3CZG[A] α-D-Glcp 1.80 3CZK[A] α-D-Glcp-(1-2)-β-D-Fruf 2.20 3CZL[A] α-D-Glcp 2.00	4
sucrose hydrolase (SuxB;XCC3359)	3.2.1.48	Xanthomonas campestris pv. campestris str. ATCC 33913	AAM42629.1 NP_638705.1	Q8P5I2	2WPG[A] 1.90	4
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