Glycoside Hydrolase Family 13 / Subf 4

Activities in Familyα-amylase (EC 3.2.1.1); pullulanase (EC 3.2.1.41); cyclomaltodextrin glucanotransferase (EC 2.4.1.19); cyclomaltodextrinase (EC 3.2.1.54); trehalose-6-phosphate hydrolase (EC 3.2.1.93); oligo-α-glucosidase (EC 3.2.1.10); maltogenic amylase (EC 3.2.1.133); neopullulanase (EC 3.2.1.135); α-glucosidase (EC 3.2.1.20); maltotetraose-forming α-amylase (EC 3.2.1.60); isoamylase (EC 3.2.1.68); glucodextranase (EC 3.2.1.70); maltohexaose-forming α-amylase (EC 3.2.1.98); maltotriose-forming α-amylase (EC 3.2.1.116); branching enzyme (EC 2.4.1.18); trehalose synthase (EC 5.4.99.16); 4-α-glucanotransferase (EC 2.4.1.25); maltopentaose-forming α-amylase (EC 3.2.1.-) ; amylosucrase (EC 2.4.1.4) ; sucrose phosphorylase (EC 2.4.1.7); malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141); isomaltulose synthase (EC 5.4.99.11); malto-oligosyltrehalose synthase (EC 5.4.99.15); amylo-α-1,6-glucosidase (EC 3.2.1.33); α-1,4-glucan: phosphate α-maltosyltransferase (EC 2.4.99.16); 6'-P-sucrose phosphorylase (EC 2.4.1.-); amino acid transporter
Activities in Sub Family
Mechanism Retaining
ClanGH-H
3D Structure Status( β / α ) 8
Catalytic Nucleophile/BaseAsp (experimental)
Catalytic Proton DonorGlu (experimental)
NoteNew: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resourcesCAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX;
Statistics GenBank accession (409); Uniprot accession (71); PDB accession (24); 3D entries (6); cryst (0)
Bacteria
Protein Name EC# OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 amylosucrase (DgAS;Dgeo_0572) 2.4.1.4 Deinococcus geothermalis DSM 11300 ABF44874.1
YP_604043.1
Q1J0W0
3UCQ[A] 1.97
3UER[A] α-D-Glcp-(1-3)-α-D-Fruf
2.10
4
 amylosucrase (AS;DrAS;DR0933) 2.4.1.4 Deinococcus radiodurans R1 AAF10510.1
ANC71873.1
NP_294657.1
Q9RVT9
4AYS[A] 3.15
4
 amylosucrase (Ams)   Neisseria polysaccharea 85322 AAM51153.1 Q84HD5
Q9ZEU2
5N7J[A] α-D-Glcp-(1-2)-β-D-Fruf
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.00
4
 amylosucrase (Ams;NpAS) 2.4.1.4 Neisseria polysaccharea ATCC 43768 AAT15258.1
AAW13710.1
ACK26675.1
CAA03058.1
CAA03083.1
CAA09772.1
E2PEX2
Q9ZEU2
1G5A[A] 1.40
1JG9[A] β-D-Glcp
1.66
1JGI[A] α-D-Glcp-(1-2)-β-D-Fruf
2.00
1MVY[A] α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.00
1MW0[A] α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.01
1MW1[A] α-D-Glcp-(1-2)-β-D-Fruf
2.10
1MW2[A] α-D-Glcp-(1-2)-β-D-Fruf
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.10
1MW3[A] α-D-Glcp-(1-2)-β-D-Fruf
2.00
1S46[A] β-D-Glcp-(1-4)-ASP
2.20
1ZS2[A] α-D-Glcp-(1-2)-β-D-Fruf
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.16
3UEQ[A] α-D-Glcp-(1-1)-<non_carb>
α-D-Glcp-(1-3)-β-D-Fruf
1.85
4FLO[A] 2.20
4FLQ[A] 2.50
4FLR[A] 2.40
4FLS[A] α-D-Glcp-(1-2)-β-D-Fruf
2.30
4
 sucrose hydrolase (Suh) 3.2.1.- Xanthomonas axonopodis pv. glycines 8RA AAQ93678.1 Q6UVM5
3CZE[A] 1.90
3CZG[A] α-D-Glcp
1.80
3CZK[A] α-D-Glcp-(1-2)-β-D-Fruf
2.20
3CZL[A] α-D-Glcp
2.00
4
 sucrose hydrolase (SuxB;XCC3359) 3.2.1.- Xanthomonas campestris pv. campestris str. ATCC 33913 AAM42629.1
NP_638705.1
Q8P5I2
2WPG[A] 1.90
4
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