Activities in Family	Levan fructotransferase (DFA-IV-forming) (EC 4.2.2.16);
Mechanism	Retaining a
Clan	GH-J
3D Structure Status	5-fold β-propeller
Catalytic Nucleophile/Base	Asp
Catalytic Proton Donor	Glu
External resources	CAZypedia; PROSITE;
Statistics	GenBank accession (29883); Uniprot accession (300); PDB accession (85); 3D entries (25); cryst (2)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
inulin exo-β-2,1-fructosidase (InuA)	3.2.1.153	Arthrobacter sp. MN8	AGC01505.1	A0A0F6MV26	8I12[A] 1.36
endolevanase (LevB1) (partial)	3.2.1.65	Bacillus licheniformis IBT-ALM / IBt1	AHK25455.1	W8GV60	9H8I[A] 1.54
endo-β-2,6-fructanase / endo-levanase (BT1760; BT_1760)	3.2.1.65	Bacteroides thetaiotaomicron VPI-5482	AAO76867.1 NP_810673.1	Q8A6W6	6R3R[A] 1.65 6R3U[A] β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 1.90 7ZNR[A,B] β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-1)-<non_carb> β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-α-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 2.65 7ZNS[A,B] α-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 2.30 8A9Y[A,C] 3.50 8AA0[A,C] β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 3.20 8AA2[A,D] β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 3.10
C8077_07125		Bifidobacterium adolescentis 1-11	AVT45692.1		6NUM[A] 1.90 6NUN[A] β-D-Fruf 1.87
BAD_1325		Bifidobacterium adolescentis ATCC 15703	BAF40106.1		6NOB[A] 2.44
β-fructofuranosidase	3.2.1.26	Bifidobacterium longum KN29.1	ABN04092.1	A2TLS9	3PIG[A,B] 1.87 3PIJ[A,B] β-D-Fruf 1.80
β-fructofuranosidase (FperFFase;FPB0191_01269)	3.2.1.26	Frischella perrara PEB0191	AJA45090.1 BCZ08027.1	A0A0A7S0J1	7VCO[A] 1.60 7VCP[A] β-D-Fruf 2.00
exo-inulinase (InuA)	3.2.1.153	Geobacillus stearothermophilus KP1289	BAC45010.1	Q8GI55	cryst
sucrose-6-phosphate hydrolase (LgAs32; LGAS_1779)	3.2.1.26	Lactobacillus gasseri ATCC 33323 = JCM 1131	ABJ61066.1	A0A806A0W7 D1YK18	6NU7[A] 1.90 6NU8[A] β-D-Fruf 1.80
levan fructotransferase (DFA-IV-forming) (LftA)	4.2.2.16	Paenarthrobacter ureafaciens K-2032	AAF73829.1 AAX01526.1 AAX01527.1	Q9KJD0	4FFF[A,B,C,D] 2.57 4FFG[A,B,C,D] β-D-Fruf-(2-6)-β-D-Fruf <carb_like_0U8> 2.30 4FFH[A,B,C,D] α-D-Glcp-(1-2)-β-D-Fruf 2.20 4FFI[A,B,C,D] β-D-Fruf-(2-6)-β-D-Fruf β-D-Fruf-(2-6)-β-D-Fruf-(2-6)-β-D-Fruf 2.30
invertase (BfrA;Tm1414)	3.2.1.26	Thermotoga maritima MSB8	AAD36485.1 AGL50344.1 AHD18692.1 AKE29174.1 CAA04518.1 NP_229215.1	G4FFE9 O33833	1UTW[A,B,C,D,E,F] 1.90 1UYP[A,B,C,D,E,F] 1.9 1W2T[A,B,C,D,E,F] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 1.87
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
cell-wall invertase 1 (AtBFruct1;Fruct1;AtcwINV1;At3g13790)	3.2.1.26	Arabidopsis thaliana	AAK76450.1 AAL85153.1 AEE75414.1 AEE75415.1 ANM65335.1 ANM65336.1 ANM65337.1 BAB01930.1 BAH56786.1 CAA52619.1 CAA52620.1 NP_187995.1 NP_566464.1	A0A1I9LRX7 A0A5S9XBW4 C0Z257 F4JEJ0 Q43866 Q9LIB8	2AC1[A] 2.15 2OXB[A] α-D-Glcp-(1-2)-β-D-Fruf 2.60 2QQU[A] α-D-Glcp-(1-2)-β-D-Fruf 2.84 2QQV[A] α-D-Glcp-(1-2)-β-D-Fruf 3.01 2QQW[A] α-D-Glcp-(1-2)-β-D-Fruf 2.80 2XQR[A,C,E,G,I,K] β-D-Fruf 2.58
exo-inulinase (Inu1)	3.2.1.153	Aspergillus awamori var. 2250	CAC44220.1	Q96TU3	1Y4W[A] 1.55 1Y9G[A] β-D-Fruf 1.87 1Y9M[A] D-1-deoxy-Manp 1.89
endo-inulinase (Inu2)	3.2.1.7	Aspergillus ficuum ATCC 16882	CAA07345.1 ACR55693.1	C5J3N4 O94220	3RWK[X] α-D-Manp-(1-3)-α-D-Manp-(1-6)-D-1-deoxy-Manp β-D-Fruf 2.10 3SC7[X] α-D-Manp-(1-3)-α-D-Manp-(1-6)-D-1-deoxy-Manp 1.50
fructosyltransferase (FT)	2.4.1.100	Aspergillus japonicus CB05	ADK46938.1	F1ADK9 Q1W3Z7	3LDK[A] α-D-Glcp-(1-2)-β-D-Fruf 2.20 3LDR[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf 2.10 3LEM[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf 2.10 3LF7[A] 2.00 3LFI[A,B] β-D-Glcp 2.00 3LIG[A] 1.80 3LIH[A] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.20
fructosyltransferase (AKAW_06215)	2.4.1.100	Aspergillus luchuensis IFO 4308	GAA88101.1	A0ABF7PII4 G7XM46	5XH8[A] 2.10 5XH9[A] 2.30 5XHA[A] β-D-Fruf 2.10
exo-inulinase (InuE)	3.2.1.153	Aspergillus niger 12	BAD01476.1	Q76HP6	cryst
β-fructofuranosidase (BmSUC1)	3.2.1.26	Bombyx mori Fuyo x Tsukubane	BCD57653.1	A0A6F8Z6Y2	7BWB[A] 1.80 7BWC[A] α-D-Glcp-(1-2)-β-D-Fruf 1.95
fructan b-(2,1)-fructosidase; fructan 1-exohydrolase IIa (1-FEH IIa)	3.2.1.153	Cichorium intybus	CAC37922.1 AAP85536.1 AFU54056.1	Q93X60 V9LTK7	1ST8[A] 2.35 2ADD[A] α-D-Glcp-(1-2)-β-D-Fruf 2.50 2ADE[A] β-D-Fruf 2.65 2AEY[A] <carb_like_DQQ> 3.27 2AEZ[A] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf 3.05
KLMA_10518		Kluyveromyces marxianus DMKU3-1042	BAO38140.1		6J0T[A,B] 2.80
sucrose:{sucrose/fructan} 6-fructosyltransferase (6-Sst/6-Sft)	2.4.1.10 2.4.1.-	Pachysandra terminalis	CBM41476.2 CBM41476.1	E3PQS3	3UGF[A,B] 1.70 3UGG[A,B] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf 2.90 3UGH[A,B] 2.90
β-fructofuranosidase (Inv; RdINV)	3.2.1.26 2.4.1.99	Rhodotorula dairenensis CECT 1416	QFG75712.1	A0A856TAI5	8BEQ[A,B] 2.07 8BES[A,B,C,D] β-D-Fruf 1.86 8BET[A,B,C,D] α-D-Glcp-(1-2)-β-D-Fruf 2.38 8BEU[A,B,C,D] α-D-Galp-(1-6)-α-D-Glcp-(1-2)-β-D-Fruf 2.27
invertase 2 (Suc2;YIL162w)	3.2.1.26	Saccharomyces cerevisiae S288c	CAA24618.1 BAJ07819.1 CAA87030.1 NP_012104.1	D5MS22 P00724	4EQV[A,B,C,D,E,F,G,H] 3.40
invertase / β-fructofuranosidase (Inv;SoINV;Ffase)	3.2.1.26	Schwanniomyces occidentalis ATCC 26077	ADN34605.1 AAE51668.1 CAA35606.1	E5D0X5 P24133	3KF3[A,B] β-D-Fruf 1.90 3KF5[A,B] 2.90 3U14[A,B] β-D-Fruf-(2-1)-<non_carb> 2.24 3U75[A,B,C,D] α-D-Glcp-(1-2)-β-D-Fruf 2.68 6S1T[A,B] α-D-Glcp-(1-2)-β-D-Fruf 2.09 6S2B[A,B] β-D-Fruf-(2-1)-<non_carb> 1.88
β-fructofuranosidase (Inv; Xd-INV; XdINV)	3.2.1.26	Xanthophyllomyces dendrorhous ATCC MYA-131	ACL79833.1	B8YJM2 J7HDY4	5ANN[A,B] 2.14 5FIX[A,B] α-D-Glcp-(1-2)-β-D-Fruf 2.01 5FK7[A,B] 2.05 5FK8[A,B] β-D-Fruf-(2-6)-α-D-Glcp-(1-4)-α-D-Glcp 1.88 5FKB[A,B] α-D-Glcp-(1-2)-β-D-Fruf 1.78 5FKC[A,B] 1.82 5FMB[A,B] β-D-Fruf 1.91 5FMC[A,B] β-D-Fruf 1.84 5FMD[A,B] α-D-Glcp-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf-(1-2)-β-D-Fruf D-1-deoxy-Manp 1.78 5NSL[A,B] β-D-Fruf 1.70 5O47[A,B] β-D-Fruf-(2-1)-<non_carb> 1.91 6FJE[A,B] α-D-Glcp β-D-Fruf 1.85 6FJG[A,B] β-D-Fruf 1.73 6S2G[A,B] β-D-Fruf 2.03 6S2H[A,B] β-D-Fruf 1.80 6S3Z[A,B] β-D-Fruf 1.85 6S82[A,B] 1.85
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