Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1139); Uniprot accession (75); PDB accession (109); 3D entries (9); cryst (0)

Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
β-galactosidase (LacZ)	3.2.1.23	pubmed	Actinobacillus pleuropneumoniae CM5	AAB17954.1	P70753		10
β-galactosidase (Amuc_0824)	3.2.1.23	pubmed	Akkermansia muciniphila ATCC BAA-835	ACD04657.1 QTD81445.1			10
β-galactosidase (Amuc_1666)	3.2.1.23	pubmed	Akkermansia muciniphila ATCC BAA-835	ACD05484.1 QTD80199.1			10
β-galactosidase (HHJ01_10865)	3.2.1.23	pubmed	Akkermansia muciniphila CSUN-19 AmIV	MBT8796011.1			10
β-galactosidase	3.2.1.23	pubmed	Alkalilactibacillus ikkensis 517	ACO52514.1	C1KC07		10
β-galactosidase (GalA)	3.2.1.23	pubmed	Alteromonas sp. ANT48	QGZ98840.1	A0A6B9KBH1		10
β-galactosidase (Bgal)	3.2.1.23	doi	Alteromonas sp. ML117	AZK90254.1	A0A3S8S8V3		10
β-galactosidase	3.2.1.23	pubmed	Alteromonas sp. ML52	AZK90255.1	A0A3Q8SL57		10
β-galactosidase (Gal2A)	3.2.1.23	pubmed	Alteromonas sp. QD01	QPF69526.1	A0A7U3PJ41		10
β-galactosidase (BglA)	3.2.1.23	pubmed	Arthrobacter psychrolactophilus F2	BAF33372.1	Q08KN3		10
β-galactosidase (BgaS)	3.2.1.23	pubmed	Arthrobacter sp. 20B	ACI41243.1	B6EAX7		10
β-galactosidase (ArthβDG)	3.2.1.23	pubmed	Arthrobacter sp. 32cB	AHY00656.1	A0A023UGN9	6ETZ[A] 6H1P[A] 6SE8[A] 6SE9[A] 6SEA[A] 6SEB[A] 6SEC[A] 6SED[A] 6ZJP[A] 6ZJQ[A] 6ZJR[A] 6ZJS[A] 6ZJT[A] 6ZJU[A] 6ZJV[A] 6ZJW[A] 6ZJX[A]	10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Arthrobacter sp. B7	AAA69907.1	Q59140		10
β-galactosidase (LacZ)	3.2.1.23	doi	Arthrobacter sp. C2-2	CAD29775.1	Q8KRF6	1YQ2[A,B,C,D,E,F]	10
β-galactosidase (GalA)	3.2.1.23	pubmed	Arthrobacter sp. ON14	ADJ18283.1	D9IU58		10
β-galactosidase (BgaS)	3.2.1.23	pubmed	Arthrobacter sp. SB	AAQ19029.1	Q7WTU5		10
β-galactosidase (cleaves D-Galp-β-1,2-L-Acef; BT0993)	3.2.1.23	pubmed	Bacteroides thetaiotaomicron VPI-5482	AAO76100.1 NP_809906.1	Q8A924		10
β-galactosidase (BgaC;BAD_1582)	3.2.1.23	pubmed	Bifidobacterium adolescentis ATCC 15703	BAF40363.1	A1A3T0		10
β-galactosidase (BIF1;OLGA1)	3.2.1.23	pubmed	Bifidobacterium bifidum DSM 20215	CAB75342.1	Q9L3L4		10
β-galactosidase (BIF2)	3.2.1.23	pubmed	Bifidobacterium bifidum DSM 20215	CAC14565.1	Q9F4D6		10
β-galactosidase P1 (BbgI;Bbg1)	3.2.1.23	pubmed	Bifidobacterium bifidum NCIMB 41171	ABE27151.1 ABE27151.2	Q0ZI53		10
β-galactosidase K2 (BbgIV)	3.2.1.23	pubmed	Bifidobacterium bifidum NCIMB 41171	ABE00939.1	Q0ZII7		10
β-galactosidase (LacZ;Lacz6;Bbr_1552_lacZ;Bbr_1552)	3.2.1.23	pubmed	Bifidobacterium breve UCC2003	ABE96223.1			10
β-galactosidase (Lacz1; Bbr_0010)	3.2.1.23	pubmed	Bifidobacterium breve UCC2003	ABE94727.1			10
β-galactosidase (Bdg2A)	3.2.1.23	pubmed	Bifidobacterium dentium Y510	UGN49370.1			10
agarotriose-specific β-galactosidase 2A (Blon_2334) (Bga2A)	3.2.1.23	pubmed	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088	ACJ53392.1 WP_012578561.1	B7GNN6		10
β-galactosidase I (β-GalI)	3.2.1.23	pubmed	Bifidobacterium longum subsp. infantis HL96	AAL02052.1	Q93GI6		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Bifidobacterium longum subsp. longum MB219	CAB44428.1	Q9X6Y5		10
β-galactosidase (B8809_0611)	3.2.1.23	pubmed	Bifidobacterium longum subsp. longum NCIMB8809	ALO72284.1			10
β-galactosidase (B8809_1361)	3.2.1.23	pubmed	Bifidobacterium longum subsp. longum NCIMB8809	ALO73032.1			10
β-galactosidase	3.2.1.23	pubmed	Clostridium acetobutylicum	AAA23216.1	P24131		10
β-galactosidase (Bga;BgaB5)	3.2.1.23	doi	Enterobacter cloacae B5	ABB73039.1	Q2XQU3		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Enterobacter cloacae GAO	BAA07673.1	Q47077		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Erwinia rhapontici E602	AYC35409.1	A0A385ZAF4		10
β-galactosidase (LacZ;EcDH1_3262)	3.2.1.23	pubmed	Escherichia coli DH1	ACX40886.1 BAJ42187.1	C9QQT4		10
β-galactosidase Z (LacZ;Lac-Z;b0344;EO53_16565)	3.2.1.23	pubmed	Escherichia coli str. K-12 substr. MG1655	AAA24053.1 AAB18068.1 AAC73447.1 AAW06767.1 AAY73171.1 ABN72582.1 AEL16982.1 AIZ92504.1 ALI41840.1 AMC97588.1 AUG15134.1 AVI54877.1 CAA23573.1 CAC87491.1 NP_414878.1	B8LFD6 G0ZKW2 P00722 Q8VNN2	1BGL[A] 1BGM[I] 1DP0[A] 1F49[A] 1F4A[A] 1F4H[A] 1GHO[I] 1HN1[A,B,C,D] 1JYN[A] 1JYV[A] 1JYW[A] 1JYX[A] 1JYY[A] 1JYZ[I] 1JZ0[A] 1JZ1[I] 1JZ2[A] 1JZ3[A] 1JZ4[A] 1JZ5[A] 1JZ6[A] 1JZ7[A] 1JZ8[A] 1PX3[A,B,C,D] 1PX4[A,B,C,D] 3CZJ[A,B,C,D] 3DYM[A,B,C,D] 3DYO[A,B,C,D] 3DYP[A,B,C,D] 3E1F[1,2,3,4] 3I3B[A,B,C,D] 3I3D[A,B,C,D] 3I3E[A,B,C,D] 3IAP[A,B,C,D] 3IAQ[A,B,C,D] 3J7H[A,B,C,D] 3MUY[1,2,3,4] 3MUZ[1,2,3,4] 3MV0[1,2,3,4] 3MV1[1,2,3,4] 3SEP[A,B,C,D] 3T08[A,B,C,D] 3T09[A,B,C,D] 3T0A[A,B,C,D] 3T0B[A,B,C,D] 3T0D[A,B,C,D] 3T2O[A,B,C,D] 3T2P[A,B,C,D] 3T2Q[A,B,C,D] 3VD3[A,B,C,D] 3VD4[A,B,C,D] 3VD5[A,B,C,D] 3VD7[A,B,C,D] 3VD9[A,B,C,D] 3VDA[A,B,C,D] 3VDB[A,B,C,D] 3VDC[A,B,C,D] 4CKD[A,B,C,D] 4DUV[A,B,C,D] 4DUW[A,B,C,D] 4DUX[A,B,C,D] 4TTG[A,B,C,D] 4V40[A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P] 4V41[A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P] 4V44[A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P] 4V45[A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P] 5A1A[A,B,C,D] 6CVM[A,B,C,D] 6DRV[A,B,C,D] 6KUZ[A,B,C,D] 6X1Q[A,B,C,D] 7BRS[A,B,C,D] 7BTK[A,B,C,D] 8BK7[A,B,C,D] 8BK8[A,B,C,D] 8BKG[A,B,C,D] 8Q7Y[A,B,C,D] 8RI6[A,B,C,D] 8RI7[A,B,C,D] 8RI8[A,B,C,D] 8VT0[A,B,C,D]	10
evolved β-galactosidase (EbgA;b3076)	3.2.1.23	pubmed	Escherichia coli str. K-12 substr. MG1655	AAA61971.1 AAA57877.1 AAC76111.1 AAT48164.1 AIZ89904.1 ALI39224.1 AMC95983.1 AUG17794.1 AVI53249.1 AYG17960.1 CAA36274.1 NP_417547.2	P06864		10
β-galactosidase (Gal4214-1)	3.2.1.23	pubmed	Flavobacterium sp. 4214	AAX48919.1	Q2VT50		10
β-galactosidase (BglP)	3.2.1.23	pubmed	gamma proteobacterium NT2-2	AMK07416.1	A0A140D6D2		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Klebsiella pneumoniae 285	AEA30145.1	F2X2W7		10
β-galactosidase (GalB)	3.2.1.23	pubmed	Klebsiella sp. T17-2	AMK07522.1	A0A126QG41		10
β-galactosidase (LacL+LacM)	3.2.1.23	pubmed	Lactiplantibacillus plantarum ATCC 8014	AAL09167.1 ATQ34817.1	Q938Q6		10
β-galactosidase (LacL+LacM)	3.2.1.23	pubmed	Lactiplantibacillus plantarum FUA3112	CAZ66936.1	C6H176		10
β-galactosidase (LacL+LacM,lp_3483+lp_3484)	3.2.1.23	pubmed	Lactiplantibacillus plantarum WCFS1	CAD65569.1 CCC80451.1 NP_786691.1	F9UUE9		10
β-galactosidase large subunit (LacL)	3.2.1.23	pubmed	Lactobacillus acidophilus ATCC 4356	ACC38286.1	B2LWG2		10
β-galactosidase Z	3.2.1.23	pubmed	Lactobacillus delbrueckii	AAA25240.1 AAA25244.1 AAY08349.1 ACW22137.1 CAC07488.1	P0C1Y0 Q1G9Z4		10
β-galactosidase Z (LacZ; Lbulagal; Ldb1201)	3.2.1.23	pubmed	Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002	CAI98003.1 AEN14565.1	Q1G9Z4		10
β-galactosidase (BgaL3)	3.2.1.23	doi	Lactobacillus delbrueckii subsp. bulgaricus L3	ACE06986.1	B3GS90		10
β-galactosidase (LacL; EcoliBL21Lhβ-gal) (large subunit)	3.2.1.23	pubmed	Lactobacillus helveticus DSM 20075 = CGMCC 1.1877 ATCC 15009	CAD55499.1	Q7WTB4		10
β-galactosidase large subunit (LacL;WANG_0293)	3.2.1.23	pubmed	Lactobacillus kefiranofaciens ZW3	AEG39988.1			10
β-galactosidase (LacZ)	3.2.1.23	doi	Lactococcus lactis ATCC 7962	AAD11504.1 AAC63020.1 CAA56341.1	O87523 Q48727		10
β-galactosidase (LacZ;L0025)	3.2.1.23	pubmed	Lactococcus lactis subsp. lactis Il1403	AAK06078.1 NP_268137.2	Q48727		10
β-galactosidase L+S (LacL+LacS) (heterodimer)	3.2.1.23	pubmed	Latilactobacillus sakei subsp. sakei DSM 20017	CAA57730.1	Q48846		10
β-galactosidase L+M (LacL+LacM)	3.2.1.23	pubmed	Leuconostoc lactis	AAA25267.1 CAL48858.1	Q02603		10
β-galactosidase (LVIS_2259 + LVIS_2258)	3.2.1.23	pubmed	Levilactobacillus brevis ATCC 367	ABJ65308.1	Q03NB4		10
β-galactosidase (LacLM;LacL+LacM)	3.2.1.23	pubmed	Limosilactobacillus fermentum K4	AEJ32720.1	F8T7X0		10
β-galactosidase (Lreubgal) (LacL + LacM)	3.2.1.23	pubmed	Limosilactobacillus reuteri L103	ABF72116.1	Q19R72		10
β-galactosidase (BgaB;MsBgaB;MS0749)	3.2.1.23	pubmed	[Mannheimia] succiniciproducens MBEL55E	AAU37356.1	Q65UK4		10
β-galactosidase (BgaA;MsBgaA;MS0806)	3.2.1.23	pubmed	[Mannheimia] succiniciproducens MBEL55E	AAU37413.1	Q65UE7		10
β-galactosidase (PbBGal2A)	3.2.1.23	pubmed	Paenibacillus barengoltzii CAU904	AJP16550.1	A0A0C5GSQ2	9M0E[A,B,C,D,E,F]	10
β-galactosidase	3.2.1.23	pubmed	Pantoea agglomerans B1	ABN42680.1	A3FEW8		10
β-1,3-galactosidase (tolerant for 6O-methylation) (BpGH2C; BACPLE_01706)	3.2.1.23	doi	Phocaeicola plebeius DSM 17135	EDY95440.1	B5CYA9		10
β-galactosidase (BgaBM)	3.2.1.23	pubmed	Priestia megaterium 2-37-4-1	ABN13675.1	A3FFK4		10
β-galactosidase (BgaM)	3.2.1.23	pubmed	Priestia megaterium DSM 319	CAA04267.1 ADF38979.1	O52847		10
β-galactosidase (MbgA;BG04_4479)	3.2.1.23	pubmed	Priestia megaterium NBRC 15308 = ATCC 14581	AJI24329.1 AAC34375.1	A0A0B6AWT5 O85167		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Pseudoalteromonas haloplanktis TAE 79	CAA10470.1 AAU97746.1	P81650		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Pseudoalteromonas sp. 22b	AAR92204.1	Q6RGS1		10
β-galactosidase	3.2.1.23	pubmed pubmed	Saccharopolyspora rectivirgula	BAA34817.1	Q9ZBD1		10
β-galactosidase	3.2.1.23	pubmed pubmed	Saccharopolyspora rectivirgula	BAA34817.1	Q9ZBD1		10
β-galactosidase (LacH)	3.2.1.23	pubmed	Staphylococcus xylosus	CAA74937.1	O33815		10
β-galactosidase (LacZ)	3.2.1.23	pubmed	Streptococcus thermophilus FUA3194	CAZ66940.1	C6H180		10
β-galactosidase (STER_1366)	3.2.1.23	pubmed	Streptococcus thermophilus LMD-9	ABJ66539.1	Q03JT3		10
β-galactosidase (SCO3479; SCE65.15c)	3.2.1.23	pubmed	Streptomyces coelicolor A3(2)	CAB61803.1 NP_627682.1	Q9RKF8		10
β-galactosidase 2C (Bga2C; Lacz2; Cst_c06190)	3.2.1.23	pubmed	Thermoclostridium stercorarium subsp. stercorarium DSM 8532	AGC67637.1 AGI38685.1	L7VLR6		10
β-galactosidase Z (LacZ;TmLac;TM1193)	3.2.1.23	pubmed	Thermotoga maritima MSB8	AAD36268.1 AAA50597.1 AGL50124.1 AHD18900.1 AKE28970.1 CAA04513.1 NP_228998.1	G4FEF3 Q56307	6S6Z[A,B,C,D,E,F,G,H] 6SD0[A,B,C,D]	10
Eukaryota
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
intracellular β-galactosidase (BgaD)	3.2.1.23	pubmed	Aspergillus nidulans WG355	ADM36015.1	G8Z4I7		10
β-galactosidase (Lac4;BgaL)	3.2.1.23	pubmed	Kluyveromyces lactis CBS2359	AAA35265.1	P00723	3OB8[A,B,C,D] 3OBA[A,B,C,D]	10
β-galactosidase (BglKL)	3.2.1.23	pubmed	Kluyveromyces lactis F61	AHW49176.1	X5D4M1		10
β-galactosidase (BgaL;KLLA0B14883g)	3.2.1.23	pubmed	Kluyveromyces lactis NRRL Y-1140	CAH02587.1	P00723		10
β-galactosidase	3.2.1.23	pubmed	Kluyveromyces marxianus DSM5418	AAS18310.1	Q6QTF4		10
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