Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
External resources	CAZypedia; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (1139); Uniprot accession (75); PDB accession (109); 3D entries (9); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-galactosidase (ArthβDG)	3.2.1.23	Arthrobacter sp. 32cB	AHY00656.1	A0A023UGN9	6ETZ[A] 1.80 6H1P[A] 3.01 6SE8[A] 1.84 6SE9[A] β-D-Fruf β-D-Galp β-D-Galp-(1-4)-β-D-Glcp 1.97 6SEA[A] β-D-Galp β-D-Galp-(1-4)-β-D-Glcp 1.87 6SEB[A] β-D-Galp1S-(1-1)-<non_carb> 2.27 6SEC[A] β-D-Galp-(1-1)-<non_carb> 2.77 6SED[A] β-D-Galp 2.23 6ZJP[A] 1.85 6ZJQ[A] β-D-Galp 1.70 6ZJR[A] 2.00 6ZJS[A] β-D-Galp 1.50 6ZJT[A] β-D-Galp β-D-Galp-(1-4)-β-D-Fruf 1.97 6ZJU[A] α-D-Glcp-(1-2)-β-D-Fruf 1.75 6ZJV[A] 2.25 6ZJW[A] β-D-Galp 2.12 6ZJX[A] 2.21	10
β-galactosidase (LacZ)	3.2.1.23	Arthrobacter sp. C2-2	CAD29775.1	Q8KRF6	1YQ2[A,B,C,D,E,F] 1.90	10
BT3179		Bacteroides thetaiotaomicron VPI-5482	AAO78285.1 NP_812091.1		3DEC[A] 2.80	10
BT1626		Bacteroides thetaiotaomicron VPI-5482	AAO76733.1 NP_810539.1		3BGA[A,B] 2.10	10
RG69_19155 (LacZ)		Escherichia coli S40	APL67431.1		6TSH[A,B,C,D] <carb_like_DGJ> 2.30 6TSK[A,B,C,D] β-L-Ribp 2.30 6TTE[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.20	10
β-galactosidase Z (LacZ;Lac-Z;b0344;EO53_16565)	3.2.1.23	Escherichia coli str. K-12 substr. MG1655	AAA24053.1 AAB18068.1 AAC73447.1 AAW06767.1 AAY73171.1 ABN72582.1 AEL16982.1 AIZ92504.1 ALI41840.1 AMC97588.1 AUG15134.1 AVI54877.1 CAA23573.1 CAC87491.1 NP_414878.1	B8LFD6 G0ZKW2 P00722 Q8VNN2	1BGL[A] 2.50 1BGM[I] 2.50 1DP0[A] 1.70 1F49[A] 2.50 1F4A[A] 2.80 1F4H[A] 2.80 1GHO[I] 2.50 1HN1[A,B,C,D] 3.00 1JYN[A] β-D-Galp-(1-4)-β-D-Glcp 1.80 1JYV[A] β-D-Galp-(1-1)-<non_carb> 1.75 1JYW[A] β-D-Galp-(1-1)-<non_carb> 1.55 1JYX[A] β-D-Galp1S-(1-1)-<non_carb> 1.75 1JYY[A] β-D-Galp2F-(1-4)-β-D-Glcp 2.70 1JYZ[I] β-D-Galp2F-(1-4)-β-D-Glcp 2.70 1JZ0[A] α-D-Galp2F-(1-4)-GLU 2.60 1JZ1[I] α-D-Galp2F-(1-4)-GLU 2.60 1JZ2[A] α-D-Galp2F-(1-4)-GLU 2.10 1JZ3[A] α-D-2-deoxy-Galp-(1-4)-GLU 1.75 1JZ4[A] α-D-2-deoxy-Galp-(1-4)-GLU 2.10 1JZ5[A] α-D-Galp β-D-Galp 1.80 1JZ6[A] <carb_like_GTZ> 2.10 1JZ7[A] β-D-Galp 1.50 1JZ8[A] β-D-Galp-(1-6)-β-D-Glcp β-D-Galp-(1-6)-β-D-Manp 1.50 1PX3[A,B,C,D] 1.60 1PX4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.60 3CZJ[A,B,C,D] β-D-Galp 2.05 3DYM[A,B,C,D] 2.05 3DYO[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.80 3DYP[A,B,C,D] 1.75 3E1F[1,2,3,4] 3.00 3I3B[A,B,C,D] α-D-Galp β-D-Galp 2.20 3I3D[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.20 3I3E[A,B,C,D] 2.10 3IAP[A,B,C,D] 2.00 3IAQ[A,B,C,D] 2.70 3J7H[A,B,C,D] 3.20 3MUY[1,2,3,4] 2.50 3MUZ[1,2,3,4] 1.90 3MV0[1,2,3,4] 2.20 3MV1[1,2,3,4] 2.20 3SEP[A,B,C,D] 2.05 3T08[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.00 3T09[A,B,C,D] α-D-Galp β-D-Galp 1.75 3T0A[A,B,C,D] 1.90 3T0B[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.40 3T0D[A,B,C,D] α-D-Galp β-D-Galp 1.93 3T2O[A,B,C,D] 1.85 3T2P[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.60 3T2Q[A,B,C,D] α-D-Galp β-D-Galp 2.40 3VD3[A,B,C,D] 2.80 3VD4[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.00 3VD5[A,B,C,D] 2.70 3VD7[A,B,C,D] <carb_like_GTZ> 2.87 3VD9[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.05 3VDA[A,B,C,D] 2.50 3VDB[A,B,C,D] α-D-Galp β-D-Galp 2.05 3VDC[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.55 4CKD[A,B,C,D] 13.00 4DUV[A,B,C,D] α-D-2-deoxy-Galp-(1-4)-GLU 2.10 4DUW[A,B,C,D] β-D-Galp-(1-6)-β-D-Glcp 2.20 4DUX[A,B,C,D] β-L-Ribp 2.30 4TTG[A,B,C,D] 1.60 4V40[A,B,C,D,E,F,G,H,I,J, 4V41[A,B,C,D,E,F,G,H,I,J, 4V44[A,B,C,D,E,F,G,H,I,J, 4V45[A,B,C,D,E,F,G,H,I,J, 5A1A[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 2.20 6CVM[A,B,C,D] β-D-Galp1S-(1-1)-<non_carb> 1.90 6DRV[A,B,C,D] 2.20 6KUZ[A,B,C,D] β-D-Galp-(1-1)-<non_carb> 2.83 6X1Q[A,B,C,D] 1.80 7BRS[A,B,C,D] β-D-Galp-(1-1)-<non_carb> 2.67 7BTK[A,B,C,D] β-D-Galp-(1-1)-<non_carb> 2.70 8BK7[A,B,C,D] 3.30 8BK8[A,B,C,D] 2.90 8BKG[A,B,C,D] 3.20 8Q7Y[A,B,C,D] 2.60 8RI6[A,B,C,D] 2.06 8RI7[A,B,C,D] 8RI8[A,B,C,D] 8VT0[A,B,C,D] 3.58	10
β-galactosidase (PbBGal2A)	3.2.1.23	Paenibacillus barengoltzii CAU904	AJP16550.1	A0A0C5GSQ2	9M0E[A,B,C,D,E,F] 2.80	10
β-galactosidase Z (LacZ;TmLac;TM1193)	3.2.1.23	Thermotoga maritima MSB8	AAD36268.1 AAA50597.1 AGL50124.1 AHD18900.1 AKE28970.1 CAA04513.1 NP_228998.1	G4FEF3 Q56307	6S6Z[A,B,C,D,E,F,G,H] 2.10 6SD0[A,B,C,D] 3.70	10
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
β-galactosidase (Lac4;BgaL)	3.2.1.23	Kluyveromyces lactis CBS2359	AAA35265.1	P00723	3OB8[A,B,C,D] β-D-Galp 2.80 3OBA[A,B,C,D] 2.75	10
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