Glycoside Hydrolase Family 3

Activities in Familyβ-glucosidase (EC 3.2.1.21); xylan 1,4-β-xylosidase (EC 3.2.1.37); β-glucosylceramidase (EC 3.2.1.45); β-N-acetylhexosaminidase (EC 3.2.1.52); α-L-arabinofuranosidase (EC 3.2.1.55); glucan 1,4-β-glucosidase (EC 3.2.1.74); isoprimeverose-producing oligoxyloglucan hydrolase (EC 3.2.1.120); coniferin β-glucosidase (EC 3.2.1.126); exo-1,3-1,4-glucanase (EC 3.2.1.-); β-N-acetylglucosaminide phosphorylases (EC 2.4.1.-); β-1,2-glucosidase (EC 3.2.1.-); β-1,3-glucosidase (EC 3.2.1.-); xyloglucan-specific exo-β-1,4-glucanase / exo-xyloglucanase (EC 3.2.1.155)
Mechanism Retaining
Catalytic Nucleophile/BaseAsp (experimental);
Catalytic Proton DonorGlu for hydrolases (experimental); histidine for phosphorylases (experimental)
NoteA recent paper by Macdonald et al. (PMID=25533455) has shown that some enzymes that cleave b-hexosaminides are in fact retaining b-glycoside phosphorylases.
External resourcesCAZypedia; HOMSTRAD; PRINTS; PROSITE;
Commercial Enzyme Provider(s)MEGAZYME; PROZOMIX ;
Statistics GenBank accession (33291); Uniprot accession (2560); PDB accession (148); 3D entries (53); cryst (4)
All (32336) Archaea (130) Bacteria (30490) Eukaryota (1611) Viruses (1) unclassified (104) Structure (53 - 4 cryst) Characterized (313)
| 1 | 2 |
Archaea
Protein Name EC#OrganismGenBank UniprotPDB/3D
 SSOP1_3127 (fragment)   Saccharolobus solfataricus P1 SAI86681.1    
 SSOP1_3128 (fragment)   Saccharolobus solfataricus P1 SAI86682.1    
 β-xylosidase / α-L-arabinosidase (XarS;SSO3032) 3.2.1.37
3.2.1.55
Saccharolobus solfataricus P2 AAK43134.1
NP_344344.1
Q97UI4  
 SULZ_04195   Saccharolobus solfataricus SUL120 AZF83362.1    
 SULA_0810   Saccharolobus solfataricus SULA AKA78566.1    
 SULB_0812   Saccharolobus solfataricus SULB AKA73176.1    
 L593_01725   Salinarchaeum sp. Harcht-Bsk1 AGN00298.1    
 L593_08905   Salinarchaeum sp. Harcht-Bsk1 AGN01726.1    
 L593_07345   Salinarchaeum sp. Harcht-Bsk1 AGN01414.1    
 C2R22_01145   Salinigranum rubrum GX10 AUV80434.1    
 HS1genome_1643 (fragment)   Sulfodiicoccus acidiphilus HS-1 BBD73254.1    
 HS1genome_1644 (fragment)   Sulfodiicoccus acidiphilus HS-1 BBD73255.1    
 SiH_2262   Sulfolobus islandicus HVE10/4 ADX83603.1    
 LD85_2613   Sulfolobus islandicus L.D.8.5 ADB88231.1 D2PG91  
 LS215_2494   Sulfolobus islandicus L.S.2.15 ACP36454.1 C3ML52  
 SiL_2171   Sulfolobus islandicus LAL14/1 AGJ63611.1    
 M1425_2322   Sulfolobus islandicus M.14.25 ACP39052.1 C3MSA9  
 M1627_2401   Sulfolobus islandicus M.16.27 ACP56257.1 C3N1N0  
 M164_2324   Sulfolobus islandicus M.16.4 ACR42924.1 C4KKR2  
 SiRe_2208   Sulfolobus islandicus REY15A ADX86262.1    
 YG5714_2461   Sulfolobus islandicus Y.G.57.14 ACP46703.1 C3NAB0  
 YN1551_0446   Sulfolobus islandicus Y.N.15.51 ACP47608.1 C3NL94  
 BFU36_07155   Sulfolobus sp. A20 AOL16510.1    
 GFS03_12515   Sulfolobus sp. E5-1-F QGA55335.1    
 N186_00235   Thermofilum adornatum 1910b AGT34445.1    
 TCARB_1127   Thermofilum adornatus 1505 AJB42175.1    
 β-glucosidase (Bgl;TpBgl;Tpen_1494) 3.2.1.21 Thermofilum pendens Hrk 5 ABL78891.1 A1S0B1  
 MA03_03765   Thermofilum uzonense 1807-2 AKG38579.1    
 ALOHA_HF4000APKG10I20ctg7g21   uncultured marine crenarchaeote HF4000_APKG10I20 ABZ10240.1 B3TCC5  
 ALOHA_HF4000APKG8D22ctg7g1   uncultured marine crenarchaeote HF4000_APKG8D22 ABZ09512.1 B3TAA3  
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