Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] pullulanase (EC 3.2.1.41);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (6); Uniprot accession (12);

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
A5888_004004		Enterococcus sp. 9E7_DIV0242	WYJ92231.1			39
DOK78_001585		Enterococcus sp. DIV2402	WYJ76948.1			39
CXP51_10120		Ethanoligenens harbinense B49	AYF39228.1			39
DRA42_10260		Ethanoligenens harbinense W1	QCN92807.1			39
CN246_10670		Ethanoligenens harbinense X-29	AYF42052.1			39
Ethha_1997		Ethanoligenens harbinense YUAN-3	ADU27515.1 AVQ96567.1			39
Q5O14_17275		Eubacteriaceae bacterium ES2	WKY44339.1			39
Q5O24_00345		Eubacteriaceae bacterium ES3	WKY47813.1			39
EUCAG14_06550 (ApU)		Eubacterium callanderi DSM 107592	WPK75121.1			39
EUCA2A_12720 (ApU)		Eubacterium callanderi DSM 2593	WPK67116.1			39
EUCA11A_12720 (ApU)		Eubacterium callanderi DSM 2594	WPK71414.1			39
EUCAMar_22640 (ApU)		Eubacterium callanderi DSM 3468	WPK84715.1			39
B2M23_07160		Eubacterium limosum ATCC 8486	ARD65330.1			39
M5595_10260 (fragment)		Eubacterium limosum B2	UQZ24596.1			39
ELI_3876		Eubacterium limosum KIST612	ADO38823.1			39
ACH52_0652		Eubacterium limosum SA11	ALU13481.1			39
EUMA32_04660 (ApU)		Eubacterium maltosivorans DSM 20517	WPK79067.1			39
CPZ25_009830		Eubacterium maltosivorans YI	QCT71612.1			39
EUS_22330		[Eubacterium] siraeum 70/3	CBK97234.1			39
NQ549_01635		[Eubacterium] siraeum DSM 15702	UWP25564.1			39
ES1_08330		[Eubacterium] siraeum V10Sc8a	CBL33914.1			39
NQ558_01190		Eubacterium ventriosum ATCC 27560	UWP36213.1			39
GXM22_05400		Faecalibacterium duncaniae JCM 31915	QIA42555.1			39
NKF69_09510 (fragment)		Faecalibacterium duncaniae NB2B-19-DCM	UTB39526.1			39
NKF69_09505 (fragment)		Faecalibacterium duncaniae NB2B-19-DCM	UTB39525.1			39
C3706_02540		Faecalibacterium prausnitzii 942/30-2	AXA81210.1			39
CG447_03530		Faecalibacterium prausnitzii A2165	ATO99049.1			39
C4Q21_08235		Faecalibacterium prausnitzii APC918/95b	AXB28912.1			39
CRH10_02830		Faecalibacterium prausnitzii Indica	ATL89317.1			39
FP2_15880		Faecalibacterium prausnitzii L2-6 L2/6	CBK99060.1			39
PXW01_05565		Faecalibacterium sp. BCRC 81399	WWE31316.1			39
MTP37_09150		Faecalibacterium sp. HTF-F	UQK55802.1			39
MTP42_04915		Faecalibacterium sp. I2-3-92	UQK49332.1			39
MTP39_08795		Faecalibacterium sp. I3-3-33	UQK44682.1			39
MTP38_09035		Faecalibacterium sp. I3-3-89	UQK42247.1			39
MTP36_06555		Faecalibacterium sp. I4-1-79	UQK41459.1			39
MTP41_13920		Faecalibacterium sp. I4-3-84	UQK36927.1			39
MTP43_08625		Faecalibacterium sp. IP-1-18	UQK52867.1			39
MTP40_05275		Faecalibacterium sp. IP-3-29	UQK52163.1			39
I6G91_04550		Fannyhessea vaginae FDAARGOS_934	QPR41476.1			39
ABE41_018805		Fictibacillus arsenicus G25-54	ANX14067.1			39
ABE65_019795		Fictibacillus phosphorivorans G25-29	ANC78922.1			39
N510_002436 (ApU)		Firmicutes bacterium ASF500	USF27489.1			39
GXM20_02670		Flavonifractor plautii JCM 32125	QIA29536.1			39
LCR02_12650		Flavonifractor plautii NBRC 113805	UBS59758.1			39
K5I25_14450		Flavonifractor plautii VE303-08	UOX44364.1			39
A4U99_16945		Flavonifractor plautii YL31	ANU42650.1 QQR04449.1 UQA25243.1			39
KIW23_01230		Floricoccus penangensis ML061-4	URZ87701.1			39
FOC48_02845		Gemella haemolysans FDAARGOS_740	QIX87760.1			39
NCTC10459_01081 (ApU)		Gemella haemolysans NCTC10459	VEI38828.1			39
FOC49_05190		Gemella morbillorum FDAARGOS_741	QGS09308.1			39
NCTC11323_01030 (ApU)		Gemella morbillorum NCTC11323	SQH55560.1			39
FOC50_07830		Gemella sanguinis FDAARGOS_742	QGS08177.1			39
CG018_06380		Gemella sp. ND 6198	AXI27051.1			39
AXE85_04255		Gemella sp. oral taxon 928 W2231	AME09415.1			39
M493_16425		Geobacillus genomosp. 3 JF8	AGT33499.1			39
GK3180		Geobacillus kaustophilus HTA426	BAD77465.1 CAZ78790.1			39
E5Z46_01270		Geobacillus kaustophilus NBRC 102445	QCK81118.1			39
GLN3_08520		Geobacillus lituanicus N-3	ASS87130.1			39
RA955_18195		Geobacillus proteiniphilus T-6	WMJ16506.1			39
GC56T3_3189		Geobacillus sp. C56-T3	ADI28114.1			39
GHH_c32540		Geobacillus sp. GHH01	AGE23745.1			39
IB49_08105		Geobacillus sp. LC300	AKU26420.1			39
GYMC52_3302		Geobacillus sp. Y412MC52	ADU95654.1			39
GYMC61_3272		Geobacillus sp. Y412MC61	ACX79826.1			39
GT50_07795		Geobacillus stearothermophilus 10	ALA70121.1			39
QSJ10_14105		Geobacillus stearothermophilus ATCC 12980	WJM12632.1			39
α-amylase / high-molecular-mass amylase (Hmma;HmwA)	3.2.1.1	Geobacillus stearothermophilus ATCC 12980	ABR26448.1	A6N9J4		39
EPB69_15795		Geobacillus stearothermophilus B5	QHN50487.1			39
QT235_17115		Geobacillus stearothermophilus EF60045	WJQ06879.1			39
QT238_17095		Geobacillus stearothermophilus EF60134	WJQ13802.1			39
amylopullulanase (ApuTS)	3.2.1.1 3.2.1.41	Geobacillus stearothermophilus TS-23	AAG44799.1 CAZ78886.1	Q9EZZ4		39
GT3570_15515		Geobacillus thermoleovorans KCTC 3570	AMV12324.1			39
amylopullulanase (Apu; Apu105; Gt-Apu)	3.2.1.1 3.2.1.41	Geobacillus thermoleovorans NP33	AFI70750.1	I1WWV6		39
C1N76_09280		Geobacillus thermoleovorans SGAir0734	AWO74683.1			39
IC805_10525		Geobacillus thermoleovorans SURF-48B	QNU20033.1			39
QWV57_16115		Geobacillus zalihae JS1-2	WKA47163.1			39
IC806_09190		Geobacillus zalihae SURF-114	QNU23368.1			39
Gferi_20625		Geosporobacter ferrireducens IRF9	AOT71727.1			39
MY490_02630		Gottfriedia acidiceleris LH14	UPM54787.1			39
Curi_c06010 (ApU)		Gottschalkia acidurici 9a	AFS77675.1			39
SANA_15970		Gottschalkiaceae bacterium SANA	BES65158.1			39
NQ540_05540		Granulicatella adiacens ATCC 49175	UWP37393.1			39
N8I82_05505		Granulicatella adiacens KHUD_009	UXY40674.1			39
MUO15_14755		Halobacillus amylolyticus SSHM10-5	UOR10862.1			39
HBHAL_4988		Halobacillus halophilus DSM 2266	CCG47325.1			39
CEH05_19565		Halobacillus halophilus HL2HP6	ASF41242.1			39
HLI_12950		Halobacillus litoralis ERB 031	QAS53031.1			39
LC065_05505		Halobacillus litoralis SaN21-8	WLR48641.1			39
HM131_02470		Halobacillus mangrovi KTB 131	ARI75759.1			39
P9989_20820		Halobacillus naozhouensis KCTC 13234	WFT74753.1			39
MUN89_03455		Halobacillus salinarum SSBR10-3	UOQ45022.1			39
MUO14_11065		Halobacillus shinanisalinarum SSTM10-2	UOQ95418.1			39
QRD89_18280		Halobacillus sp. ACCC02827	WJE15644.1			39
Haur_4335		Herpetosiphon aurantiacus DSM 785	ABX06967.1			39
A4V00_13940		Hungateiclostridiaceae bacterium KB18	ANU56099.1			39
RX717_14735		Intestinibacillus sp. NTUH-41-i26	WOC75214.1			39
NQ514_11130		Intestinibacter bartlettii DSM 16795	UWO80463.1			39
IB211_00224		Intestinimonas butyriciproducens AF211	ALP92620.1			39
SRB521_00143		Intestinimonas butyriciproducens SRB-521-5-I	QBB64407.1			39
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