Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] pullulanase (EC 3.2.1.41);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (6); Uniprot accession (12);

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
CMR01_14825		Clostridium perfringens LLY_N11	ATD50001.1			39
OEG88_00470		Clostridium perfringens MFDS1012647	UYC93101.1			39
MN196_00455		Clostridium perfringens ML2	UNM60557.1			39
NCTC13170_00094 (ApU)		Clostridium perfringens NCTC13170	SQG37362.1			39
NCTC2837_00098 (ApU)		Clostridium perfringens NCTC2837	SQI02037.1			39
PVA22_00485		Clostridium perfringens QHY-2	WDT39627.1			39
CPR_0084		Clostridium perfringens SM101	ABG86305.1			39
CPE0066		Clostridium perfringens str. 13	BAB79772.1 CAY02923.1 CAZ78718.1 NP_560982.1			39
CSACC_43490 (ApU)		Clostridium saccharobutylicum BAS/B3/SW/136	AQS02499.1			39
CLSA_c43790		Clostridium saccharobutylicum DSM 13864	AGX45322.1			39
CLOSACC_43490 (ApU)		Clostridium saccharobutylicum NCP 195	AQS16482.1			39
CLOSC_43440 (ApU)		Clostridium saccharobutylicum NCP 200	AQR92597.1			39
CLOBY_42780 (ApU)		Clostridium saccharobutylicum NCP 258	AQS12101.1			39
Cspa_c55800 (AmyB)		Clostridium saccharoperbutylacetonicum N1-4(HMT)	AGF59311.1			39
CLSAP_53290 (ApU)		Clostridium saccharoperbutylacetonicum N1-504	AQR97982.1			39
CSCA_2673		Clostridium scatologenes ATCC 25775	AKA69798.1			39
CP523_06785		Clostridium septicum DSM 7534	AYE34191.1			39
NH397_15105		Clostridium septicum RMA 8861	USS00774.1			39
EI377_01260		Clostridium septicum VAT12	QAS59555.1			39
Q6375_15325		Clostridium septicum WW106	WLF69316.1			39
GOM42_00525		Clostridium sp. 16K-1-R1	USQ63597.1			39
Q6H37_22255		Clostridium sp. JS66	WPC40595.1			39
RCG18_27530		Clostridium sp. OS1-26	WML34947.1			39
BGI42_13630		Clostridium taeniosporum 1/k	AOR24713.1			39
CTK_C29870		Clostridium tyrobutyricum KCTC 5387	AND86225.1			39
GTH52_12485		Clostridium tyrobutyricum L319	QNB67648.1			39
BA182_13890		Clostridium tyrobutyricum W428	ANP70716.1			39
EAV92_00075		Cohnella sp. 18JY8-7	AYQ71145.1			39
GXM19_02565		Collinsella aerofaciens ATCC 25986 JCM 10188	QIA33253.1			39
CV096_05105		Collinsella aerofaciens C11	AZH69620.1			39
CSV91_02795		Collinsella aerofaciens indica	ATP53560.1			39
LCQ44_08165		Collinsella aerofaciens NBRC 114504	UBS35391.1			39
KPC83_04655		Collinsella sp. zg1085	QWT17141.1			39
NQ498_08965		Collinsella stercoris DSM 13279	UWP11392.1			39
BTM29_03670		Companilactobacillus allii WiKim39	APX71706.1			39
M5C72_00745		Companilactobacillus allii WiKim39	USQ68794.1			39
JP39_11000		Companilactobacillus heilongjiangensis DSM 28069	ALB29836.1			39
D1B17_11830		Companilactobacillus zhachilii HBUAS52074	AYE39526.1			39
COPRO5265_0073		Coprothermobacter proteolyticus DSM 5265	ACI17347.1 ACI17347.2			39
Corgl_1074		Coriobacterium glomerans PW2	AEB07182.1			39
IM538_21825		Cytobacillus suaedae HD4P25	QOR69029.1			39
LPY66_20010		Dehalobacter sp. DCM	UWG97122.1			39
Desde_2650		Desulfitobacterium dehalogenans ATCC 51507	AFM00967.1			39
Desdi_2209		Desulfitobacterium dichloroeliminans LMG P-21439	AGA69642.1			39
DPCES_2231		Desulfitobacterium hafniense	CDX02118.1			39
Dhaf_3195		Desulfitobacterium hafniense DCB-2	ACL21215.1 CAZ78852.1			39
DSY2035		Desulfitobacterium hafniense Y51	BAE83824.1			39
DESME_08205		Desulfitobacterium metallireducens DSM 15288 853-15A	AHF07053.1			39
B0537_07160		Desulforamulus ferrireducens GSS09	AQS58883.1			39
Dred_1458		Desulforamulus reducens MI-1	ABO49988.1			39
Desru_2584		Desulforamulus ruminis DSM 2154	AEG60811.1			39
Desaci_0650		Desulfosporosinus acidiphilus SJ4	AFM39712.1			39
Desor_2280		Desulfosporosinus orientis DSM 765	AET67877.1			39
Desca_0951		Desulfotomaculum nigrificans CO-1-SRB	AEF93827.1			39
FNV33_05185		Dolosigranulum pigrum 83VPs-KB5	QDO92371.1			39
B5772_05730		Dolosigranulum pigrum KPL1914	QJS96430.1			39
B8A41_06835		Dolosigranulum pigrum KPL1934_CDC4709-98	QJS98330.1			39
FE321_04530		Dolosigranulum pigrum KPL3033	QTJ32911.1			39
FE322_04645		Dolosigranulum pigrum KPL3043	QTJ34661.1			39
FE323_05140		Dolosigranulum pigrum KPL3050	QTJ36411.1			39
FE324_05135		Dolosigranulum pigrum KPL3052	QTJ38195.1			39
FE325_04620		Dolosigranulum pigrum KPL3065	QTJ39840.1			39
FE326_05060		Dolosigranulum pigrum KPL3069	QTJ41571.1			39
FE327_04735		Dolosigranulum pigrum KPL3070	QTJ43271.1			39
FE328_02015		Dolosigranulum pigrum KPL3077	QTJ44424.1			39
FE329_04835		Dolosigranulum pigrum KPL3084	QTJ46686.1			39
FE330_04655		Dolosigranulum pigrum KPL3086	QTJ48322.1			39
FE331_05185		Dolosigranulum pigrum KPL3090	QTJ50068.1			39
FE332_04735		Dolosigranulum pigrum KPL3246	QTJ51797.1			39
FE333_04680		Dolosigranulum pigrum KPL3250	QTJ53445.1			39
FE334_02700		Dolosigranulum pigrum KPL3256	QTJ54737.1			39
FE335_01470		Dolosigranulum pigrum KPL3264	QTJ56239.1			39
FE336_04510		Dolosigranulum pigrum KPL3274	QTJ58538.1			39
FE337_04905		Dolosigranulum pigrum KPL3911	QTJ60207.1			39
KFE19_09610		Dysosmobacter sp. Marseille-Q4140	QUO36689.1			39
EIO64_10165		Dysosmobacter welbionis J115	QCI59536.1			39
MUG87_15330		Ectobacillus sp. JY-23	UOY91830.1			39
EH197_14445		Enterococcus avium 352	QCQ13322.1			39
AUF16_02710		Enterococcus avium FDAARGOS_184	AYQ23620.1			39
G15_2167 (ApU)		Enterococcus avium G-15	BBM18501.1			39
CIRMBP1274_01474		Enterococcus cecorum isolate CIRMBP-1274	CAI3393392.1			39
CIRMBP1212_01286		Enterococcus cecorum isolate CIRMBP-1212	CAI3366990.1			39
CIRMBP1281_01500		Enterococcus cecorum isolate CIRMBP-1281	CAI3382558.1			39
CIRMBP1228_01684		Enterococcus cecorum isolate CIRMBP-1228	CAI3405112.1			39
CIRMBP1283_01303		Enterococcus cecorum isolate CIRMBP-1283	CAI3399698.1			39
CIRMBP1261_01140		Enterococcus cecorum isolate CIRMBP-1261	CAI3355102.1			39
CIRMBP1246_01316		Enterococcus cecorum isolate CIRMBP-1246	CAI3359806.1			39
CIRMBP1287_01397		Enterococcus cecorum isolate CIRMBP-1287	CAI3517572.1			39
CIRMBP1302_01395		Enterococcus cecorum isolate CIRMBP-1302	CAI3444559.1			39
NCTC12421_01352 (ApU)		Enterococcus cecorum NCTC12421	SQE55393.1			39
PML78_13080		Enterococcus dispar K205-4b	WCG33086.1			39
EGCR1_04345		Enterococcus gilvus CR1	AXG37975.1			39
P3T75_05560		Enterococcus montenegrensis CoE-012-22	WHA10272.1			39
N7K38_07395		Enterococcus raffinosus ATCC 49464	UXK05558.1			39
K5P74_06220		Enterococcus raffinosus CX012922	QZO10075.1			39
N7K39_06655		Enterococcus raffinosus Er676	UXJ96940.1			39
J9537_06825		Enterococcus raffinosus F162_2	QXJ60479.1			39
N4S13_06730		Enterococcus raffinosus HG-5	UXC26865.1			39
EsVE80_15470 (ApU)		Enterococcus saigonensis VE80	BCA86024.1			39
CXP51_10120		Ethanoligenens harbinense B49	AYF39228.1			39
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