Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] pullulanase (EC 3.2.1.41);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (6); Uniprot accession (12);

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
DA436_04885		Clostridioides difficile 020711	AYD24167.1			39
C3348_04945		Clostridioides difficile 08-00495	AVB48946.1			39
TW87_07045		Clostridioides difficile 08ACD0030	AMM56264.1			39
C3353_04945		Clostridioides difficile 09-00072	AVB59878.1			39
C3349_04945		Clostridioides difficile 10-00071	AVB56251.1			39
C3351_04950		Clostridioides difficile 10-00078	AVB63498.1			39
C3352_04955		Clostridioides difficile 10-00253	AVB67044.1			39
C3347_04945		Clostridioides difficile 12-00008	AVB33000.1			39
C3350_04955		Clostridioides difficile 12-00011	AVB52626.1			39
KFK07_04920		Clostridioides difficile 2015	QUP84755.1			39
JMW46_14210		Clostridioides difficile 2016	QQY75295.1			39
CD0886		Clostridioides difficile 630	CAJ67719.1 AJP10558.1			39
CDIF630erm_01006 (AmyB)		Clostridioides difficile 630 delta erm	ARE61785.1			39
CD630DERM_08860		Clostridioides difficile 630Derm	CEJ97433.1			39
JMW47_06790		Clostridioides difficile 7009825	QQY73857.1			39
BN1095_630009		Clostridioides difficile 7032989	CDT66671.1			39
BN1097_350010		Clostridioides difficile 7032994	CDS84838.1			39
C4J70_04305		Clostridioides difficile AK	AVI11533.1			39
CDIF1296T_01052		Clostridioides difficile ATCC 9689 = DSM 1296	AKP41939.1			39
BZ168_13520		Clostridioides difficile BR81	AQU10635.1			39
DF008_00865		Clostridioides difficile CBA7204	QHF99232.1			39
C3W74_04940		Clostridioides difficile CD-10-00484	AVB40343.1			39
DDG63_05250		Clostridioides difficile CD161	AWH80417.1			39
JMW48_10310		Clostridioides difficile CD1801	QQY67507.1			39
CD196_0836		Clostridioides difficile CD196	CBA61597.1			39
JMW51_15975		Clostridioides difficile CD4401	QQY63105.1			39
JMW55_00680		Clostridioides difficile CD9001	QQY60247.1			39
JMW49_03740		Clostridioides difficile CD9301	QQY57405.1			39
JMW52_15525		Clostridioides difficile CD9501	QQY52824.1			39
JMW50_01640		Clostridioides difficile CD9701	QQY50243.1			39
DDG61_05520		Clostridioides difficile CDT4	AWH76650.1			39
GLV80_14405		Clostridioides difficile CFSAN096664	QOX88099.1			39
CGC51_04920		Clostridioides difficile DH/NAP11/106/ST-42	ASN88667.1			39
CDIF101085_00941		Clostridioides difficile DSM 101085	QPK99253.1			39
CDIF102859_01041		Clostridioides difficile DSM 102859	AXU26874.1			39
CDIF102860_01132		Clostridioides difficile DSM 102860	AXU30733.1			39
CDIF102978_01132		Clostridioides difficile DSM 102978	AXU34521.1			39
DSM105001_00984		Clostridioides difficile DSM 105001	QNG63983.1			39
CDIF27638_00971		Clostridioides difficile DSM 27638	AXB60063.1			39
CDIF27639_00968		Clostridioides difficile DSM 27639	AXB63752.1			39
CDIF27640_00971		Clostridioides difficile DSM 27640	AXB67508.1			39
CDIF28196_00970		Clostridioides difficile DSM 28196	AXU56360.1			39
CDIF28666_01033		Clostridioides difficile DSM 28666	AXU60115.1			39
CDIF28668_00973		Clostridioides difficile DSM 28668	AXU70924.1			39
CDIF28669_00982		Clostridioides difficile DSM 28669	AXU63645.1			39
CDIF28670_00971		Clostridioides difficile DSM 28670	AXU74584.1			39
CDIF29020_00972		Clostridioides difficile DSM 29020	AXU67303.1			39
CDIF29627_01023		Clostridioides difficile DSM 29627	AXU45513.1			39
CDIF29629_01015		Clostridioides difficile DSM 29629	AXU49212.1			39
CDIF29632_00955		Clostridioides difficile DSM 29632	AXU82131.1			39
CDIF29637_00940		Clostridioides difficile DSM 29637	AXU52717.1			39
CDIF29688_00979		Clostridioides difficile DSM 29688	AXU78347.1			39
CDIF29745_00964		Clostridioides difficile DSM 29745	AXU85810.1			39
CDIF29747_00930		Clostridioides difficile DSM 29747	AXU89474.1			39
A6J95_05310		Clostridioides difficile FDAARGOS_267	ARC14442.1			39
FOC31_09215		Clostridioides difficile FDAARGOS_723	QGS39571.1			39
JMW53_13005		Clostridioides difficile M120	QQY45511.1			39
FQN08_04725		Clostridioides difficile Mta-79	QEK80539.1			39
ERS445050_02811 (ApU)		Clostridioides difficile NCTC13307	CKH55004.1			39
CWR57_04265		Clostridioides difficile R0104a	AUA36209.1			39
C4E25_16190		Clostridioides difficile R1	AVD44206.1			39
C4E26_16180		Clostridioides difficile R2	AVD40696.1			39
CDR20291_0816		Clostridioides difficile R20291	CBE02886.1 AVB36621.1			39
CDIF27147_00973		Clostridioides difficile R20291	QPK95621.1			39
C4E42_08430		Clostridioides difficile R3	AVD35856.1			39
FTB24_14205		Clostridioides difficile TW11	QFS32455.1			39
EUU24_11880		Clostridioides difficile TW11-RT078	QIF79227.1			39
CWR54_04320		Clostridioides difficile W0003a	AUA28293.1			39
CWR55_04555		Clostridioides difficile W0022a	AUA21026.1			39
CWR56_04355		Clostridioides difficile W0023a	AUA24709.1			39
PCZ31_0905 (ApU)		Clostridioides difficile Z31	ALP02849.1			39
JJC01_15895		Clostridioides sp. ES-S-0010-02	UDN57638.1			39
JJC02_04580		Clostridioides sp. ES-S-0054-01	UDN55461.1			39
JJC16_04500		Clostridioides sp. ES-S-0107-01	UDN51938.1			39
JJJ25_04780		Clostridioides sp. ES-S-0173-01	UDN48381.1			39
IC758_04740		Clostridioides sp. ES-W-0016-02	UDN62771.1			39
CACET_c33780		Clostridium aceticum DSM 1496	AKL96821.1			39
Cbs_4906		Clostridium beijerinckii ATCC 35702 SA-1	AIU01760.1			39
CLBIJ_52340 (ApU)		Clostridium beijerinckii BAS/B3/I/124	AQS07770.1			39
PZA12_23870		Clostridium beijerinckii BRM001	WWA21399.1			39
KDJ94_25665		Clostridium beijerinckii CBEI	QUF73999.1			39
OD350_21825		Clostridium beijerinckii CloBei18h	UYZ34869.1			39
CIBE_5925		Clostridium beijerinckii DSM 6423	CUU51005.1			39
KEC93_24735		Clostridium beijerinckii DSM 791	QUN35076.1			39
LF65_07045		Clostridium beijerinckii NCIMB 14988	AMK50520.1			39
Cbei_4906		Clostridium beijerinckii NCIMB 8052	ABR37012.1			39
X276_01030		Clostridium beijerinckii NRRL B-598	ALB43962.1			39
CM240_1890		Clostridium bornimense M2/40	CDM69048.1			39
U728_2335		Clostridium botulinum 202F	AIY81730.1			39
CB17B3322		Clostridium botulinum B str. Eklund 17B (NRP)	CDH92311.1			39
CLL_A3414		Clostridium botulinum B str. Eklund 17B (NRP)	ACD21882.1			39
CbC4_1474		Clostridium botulinum BKT015925	AEB76153.1			39
IG390_05925		Clostridium botulinum BKT2873	QPW61698.1			39
CLH_3164		Clostridium botulinum E3 str. Alaska E43	ACD51807.1			39
ST13_14965		Clostridium botulinum NCTC 8266	AJF30956.1			39
ST12_14965		Clostridium botulinum NCTC 8550	AJF34018.1			39
JW813_16410		Clostridium botulinum ZJK-3	UZP03275.1			39
JYA71_16680		Clostridium botulinum ZJK-8	UZP06634.1			39
JYA74_16405		Clostridium botulinum ZJK-9	UZP10014.1			39
HLV60_00015		Clostridium butyricum 16-3	QJU42812.1			39
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