Activities in Sub Family	[retaining] α-amylase (EC 3.2.1.1); [retaining] pullulanase (EC 3.2.1.41);
Mechanism	Retaining a
Clan	GH-H
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Asp (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431)
External resources	CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS;
Commercial Enzyme Provider(s)	MEGAZYME; PROZOMIX;
Statistics	GenBank accession (6); Uniprot accession (12);

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Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D	Subf
CLOST_0971		Acetoanaerobium sticklandii DSM 519	CBH21094.1			39
DOZ58_10425		Acetobacterium sp. KB-1	AWW27013.1			39
CHL1_000314 (fragment)		Acetobacterium wieringae CH1	URN84731.1			39
CHL1_000315 (fragment)		Acetobacterium wieringae CH1	URN84732.1			39
LNN31_01735		Acetobacterium wieringae Y	UYO63190.1			39
Awo_c03460 (AmyB)		Acetobacterium woodii DSM 1030	AFA47147.1			39
Acfer_0405		Acidaminococcus fermentans DSM 20731	ADB46808.1			39
DEACI_3459 (fragment)		Acididesulfobacillus acetoxydans	CAA7602780.1			39
ADH66_14400		Acutalibacter muris KB18	ASB42775.1 QIG72395.1 QQR32000.1			39
RWV98_01810		Agathobaculum sp. NTUH-O15-33	WNX85035.1			39
amylase-pullulanase (Amy;Aaci_2874)	3.2.1.1 3.2.1.41	Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446	ACV59878.1 CAA44638.1 CAZ78868.1	C8WUR2 Q06307		39
TC41_3217		Alicyclobacillus acidocaldarius subsp. acidocaldarius Tc-4-1	AEJ45097.1			39
JZ786_21230		Alicyclobacillus ferrooxydans S30H14	QSO46921.1			39
α-amylase (AmyA4)	3.2.1.1	Alicyclobacillus sp. A4 / CGMCC 3147	ADZ99363.1	F2VRZ2		39
FY534_13270		Alicyclobacillus sp. TC	QRF24488.1			39
MOJ78_17425		Alkalihalobacillus sp. AL-G	WLD92769.1			39
Amet_2944		Alkaliphilus metalliredigens QYMF	ABR49094.1			39
bsdcttw_47050		Anaerocolumna sp. CTTW	BCK01665.1			39
ANT_30520		Anaerolinea thermophila UNI-1	BAJ65078.1			39
bsdtb5_17790		Anaeromicropila herbilytica TB5	BCN30484.1			39
AMOR_14300		Anaeromyxobacter oryzae Red232	BDG02434.1			39
V3C18_10490		Anaerostipes caccae DFI.7.76	WVP48485.1			39
EYQ97_06445		Anaerostipes caccae DSM 14662	QMW70945.1			39
ANCC_21990		Anaerostipes caccae JCM 13470	BCD36163.1			39
NQ561_11125		Anaerostipes caccae L1-92 DSM 14662	UWN70369.1			39
LCQ53_00870		Anaerostipes caccae NBRC 114412	UBS42809.1			39
RBI15_10600		Anaerostipes hadrus BA1	WMD15822.1			39
DO83_11500		Anaerostipes hadrus BPB5	AQP40143.1			39
V3C09_09520		Anaerostipes hadrus DFI.4.30	WVP57612.1			39
RBI16_10600		Anaerostipes hadrus GIF7	WMD24686.1			39
Ahadr17467_19320		Anaerostipes hadrus JCM 17467	BEG60302.1			39
AR1Y2_2314		Anaerostipes rhamnosivorans 1y2	QCP35768.1			39
P8F77_12115		Anaerostipes sp. PC18	WRY46298.1			39
NQ528_16365		Anaerotruncus colihominis DSM 17241	UWN74729.1			39
K5I23_08540		Anaerotruncus colihominis VE303-02	UOX67238.1			39
PP175_14770		Aneurinibacillus sp. Ricciae_BoGa-3	WCK52693.1			39
GFC30_469 (ApU)		Anoxybacillus amylolyticus DSM 15939	ANB61061.1			39
B379_08195		Anoxybacillus ayderensis G10	AXM89129.1			39
amylopullanase (C289_2785;ApuASK)	3.2.1.1 3.2.1.41	Anoxybacillus ayderensis SK3-4	EPZ37192.1			39
AF2641_04165		Anoxybacillus flavithermus DSM 2641T	AST06126.1			39
Aflv_2609		Anoxybacillus flavithermus WK1	ACJ34962.1			39
AFK25_13355		Anoxybacillus gonensis G2	AKS39528.1			39
CFX0092_b0285		Ardenticatena Cfx-K	CUS05819.1			39
HPK19_20870		Arthrobacter citreus NEB 577	QKE75020.1			39
ATOBIA_01060		Atopobiaceae bacterium P1	BCV18024.1			39
J4859_00605		Atopobium sp. oral taxon 416 W9116	QUC03509.1			39
CWI35_12030		[Bacillus] caldolyticus NEB414	AUI37140.1			39
LC087_12020		Bacillus carboniphilus SaN35-3	WLR41605.1			39
RGB74_01515		Bacillus sp. NEB1478	WNB92369.1			39
M662_18595		Bacillus sp. SB49	QHT48411.1			39
amylase-pullulanase (AapT)	3.2.1.1 3.2.1.41	Bacillus sp. XAL601	BAA05832.1	Q45643		39
HA075_04670		bacterium BFN5	QJW48913.1			39
HA075_04810		bacterium BFN5	QJW48918.1			39
NQ550_04560		Blautia wexlerae DSM 19850	UWO21589.1			39
BREVNS_0657 (fragment)		Brevinematales bacterium NS	QJR21407.1			39
KY493_07050		Brevundimonas sp. PAMC22021	QYF88207.1			39
JOS54_02530		Bulleidia sp. zg-1006	QRG87203.1			39
CL2_07800		butyrate-producing bacterium SSC/2	CBL37811.1			39
CLDAP_27060		Caldilinea aerophila DSM 14535 = NBRC 104270	BAM00746.1			39
CLDAP_19170		Caldilinea aerophila DSM 14535 = NBRC 104270	BAL99956.1			39
FDN13_06325		Caloramator sp. E03	QCX33355.1			39
PL321_03385 (fragment)		Caloramator sp. mosi_1	WDC84717.1			39
PL321_03380 (fragment)		Caloramator sp. mosi_1	WDC85580.1			39
PL321_03375 (fragment)		Caloramator sp. mosi_1	WDC84716.1			39
DCMF_28745		Candidatus Formimonas warabiya DCMF	ATW28215.1			39
H6X83_00280		Caproicibacterium amylolyticum LBM18003	QNO18145.1			39
PXC00_00295		Caproicibacterium argilliputei ZCY20-5	WOC32340.1			39
GJQ69_08965		Caproicibacterium lactatifermentans LBM19010	QKN24589.1			39
GJQ69_00310		Caproicibacterium lactatifermentans LBM19010	QKN23060.1			39
OP489_11660		Caproicibacterium sp. BJN0003	UZT82105.1			39
FL966_09730		Caproiciproducens galactitolivorans BS-1	QEY35300.1			39
HCR03_11440		Caproiciproducens sp. 7D4C2	QNK39367.1			39
HCR03_17615		Caproiciproducens sp. 7D4C2	QNK40439.1			39
VXK30_00235		Caproiciproducens sp. CPB-2	WVE47806.1			39
EQM14_00540		Caproiciproducens sp. NJN-50	QAT48387.1			39
Q783_07995		Carnobacterium inhibens subsp. gilichinskyi WN1359	AGY82130.1			39
CAR_c17380 (fragment)		Carnobacterium sp. 17-4	AEB30396.1			39
NY10_1127		Carnobacterium sp. CP1	ALV21736.1			39
JHE06_09020		Carnobacterium sp. CS13	QQP71479.1			39
LHA31_10615		Carnobacterium viridans AB01	UDE94993.1			39
OZ401_002293		Chloroflexota bacterium L227-S17	WJW66490.1			39
Caur_0705		Chloroflexus aurantiacus J-10-fl	ABY33944.1 CAZ78820.1			39
Chy400_0761		Chloroflexus aurantiacus Y-400-fl	ACM52191.1			39
B1H56_05205		Christensenella minuta DSM 22607	AYH39921.1			39
CE91St36_14520		Christensenellaceae bacterium CE91-St36	BDF58635.1			39
CE91St37_14530		Christensenellaceae bacterium CE91-St37	BDF61303.1			39
C12CBH8_21060		Clostridia bacterium 12CBH8	BCI61467.1			39
NQU17_06280		Clostridiaceae bacterium HFYG-1003	UUM13161.1			39
KFE18_04585		Clostridiaceae bacterium Marseille-Q4143	QUO22862.1			39
KFE16_12820		Clostridiaceae bacterium Marseille-Q4149	QUO34762.1			39
CLOSBL6_0057		Clostridiales bacterium	CAB1239245.1			39
CLOSBL4_0838		Clostridiales bacterium	CAB1243760.1			39
FRZ06_05325		Clostridiales bacterium MT110	QOX62807.1			39
BN1096_340008		Clostridioides difficile	CDS84395.1			39
DA418_04190		Clostridioides difficile 020477	AYC92852.1			39
DA422_04950		Clostridioides difficile 020482	AYC96562.1			39
DA426_14975		Clostridioides difficile 020688	AYD11557.1			39
DA430_04935		Clostridioides difficile 020695	AYD13294.1			39
DA432_04895		Clostridioides difficile 020696	AYD16959.1			39
DA434_04935		Clostridioides difficile 020709	AYD20606.1			39
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