Mechanism	Retaining a
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Unclear, possible neighboring group participation from O2 (after Thompson et al. Proc. Natl. Acad. Sci. USA., 2012, 109, 781-786)
Catalytic Proton Donor	Glu (experimental)
Note	Created after Spiro, Bhoyroo and Spiro (1997) J. Biol. Chem. 272, 29356-29363
External resources	CAZypedia;
Statistics	GenBank accession (726); Uniprot accession (8); PDB accession (40); 3D entries (3); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-α-1,2-mannosidase (BtGH99;BT3862) (BtGH99)	3.2.1.130 3.2.1.198	Bacteroides thetaiotaomicron VPI-5482	AAO78967.1 NP_812773.1	Q8A109	4ACY[A,B] 1.69 4ACZ[A,B] 1.99 4AD0[A,B,C,D] 2.09
endo-α-1,2-mannosidase (BxGH99;EndoMan;BXY_34140) (BxGH99)	3.2.1.130 3.2.1.198	Bacteroides xylanisolvens XB1A	CBK68409.1	D6D1V7	4AD1[A] 1.90 4AD2[A,B,C,D] α-D-Glcp-(1-1)-<non_carb> <carb_like_IFM> 2.10 4AD3[A,B,C,D] α-D-Glcp-(1-1)-<non_carb> <carb_like_DMJ> 2.00 4AD4[A] α-D-Glcp-(1-1)-<non_carb> α-D-Manp-(1-2)-β-D-Manp <carb_like_IFM> 1.90 4AD5[A] α-D-Glcp-(1-1)-<non_carb> α-D-Manp-(1-2)-α-D-Manp <carb_like_DMJ> 1.90 4UTF[A] α-D-Manp-(1-1)-<non_carb> α-D-Manp-(1-2)-α-D-Manp <carb_like_IFM> 1.30 4V27[A] α-D-Manp-(1-1)-<non_carb> <carb_like_IFM> 1.90 4V28[A] α-D-Manp-(1-3)-α-D-Manp-(1-1)-<non_carb> 1.20 5LYR[A] α-D-Manp-(1-1)-<non_carb> <carb_like_MNM> 1.14 5M03[A] α-D-Manp-(1-1)-<non_carb> α-D-Manp-(1-2)-α-D-Manp <carb_like_MNM> 1.05 5M17[A] α-D-Manp-(1-3)-D-1,2-deoxy-Glcp 1.03 5M3W[A] α-D-Manp-(1-2)-α-D-Manp α-D-Manp-(1-3)-D-1,2-deoxy-Glcp 1.04 5M5D[A] α-D-Manp-(1-3)-D-1,2-deoxy-Glcp 1.07 5MC8[A] α-D-Manp-(1-2)-α-D-Manp α-D-Manp-(1-3)-D-1,2-deoxy-Glcp 1.18 5MEL[A] α-D-Glcp-(1-1)-<non_carb> 1.20 6FAM[A] α-D-Manp-(1-1)-<non_carb> 1.13 6FAR[A] α-D-Manp-(1-1)-<non_carb> <carb_like_MVL> 1.30 6FWG[A] α-D-Manp-(1-3)-α-D-Manp-(1-2)-α-D-Manp-(1-2)-α-D-Manp-(1-1)-methyl 1.07 6FWI[A] α-D-Glcp-(1-1)-<non_carb> 1.25 6FWJ[A] α-D-Glcp-(1-1)-<non_carb> α-D-Manp-(1-2)-α-D-Manp 1.04 6FWL[A] α-D-Glcp-(1-1)-<non_carb> 1.12 6FWM[A] α-D-Glcp-(1-1)-<non_carb> 1.28 6FWO[A] α-D-Glcp-(1-1)-<non_carb> 1.34 6FWP[A] α-D-Manp-(1-2)-α-D-Manp α-D-Manp-(1-3)-α-D-Manp 1.08 6FWQ[A] α-D-Manp-(1-2)-α-D-Manp α-D-Manp-(1-3)-α-D-Manp 1.65 6HMG[A] α-D-Glcp-(1-1)-<non_carb> 1.27 6HMH[A] α-D-Glcp-(1-1)-<non_carb> α-D-Manp-(1-2)-α-D-Manp 1.03 6ZJ6[AAA]
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
glycoprotein endo-α-1,2-mannosidase (ManeA)	3.2.1.130	Homo sapiens	CAE12165.1 AAI46672.1 AAI50200.1 AAL07306.1 AAL07307.1 AAQ75077.1 ACH33940.1 BAB14298.1 BAF84629.1 CAD97640.1 CAE45927.1 CAH33902.1 CAI17346.1 CAI17347.1 NP_078917.2	A6H8M6 Q5SRI9 Q8WWX4	6ZDC[A] 2.25 6ZDF[A,B,C] 3.00 6ZDK[AAA] 6ZDL[AAA] 6ZFA[AAA] 6ZFN[AAA] 6ZFQ[AAA] 6ZJ1[AAA] 6ZJ5[AAA]
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