Mechanism	Retaining e
Clan	GH-A
3D Structure Status	( β / α ) 8 barrel
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	Once known as cellulase family A; many members have been assigned to subfamilies as described by Aspeborg et al. (2012) BMC Evol Biol. 12(1):186 (PMID: 22992189).
External resources	CAZypedia; HOMSTRAD; PROSITE;
Commercial Enzyme Provider(s)	MEGAZYME; NZYTech; PROZOMIX;
Statistics	GenBank accession (2595); Uniprot accession (64); PDB accession (35); 3D entries (9); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
endo-β-1,4-glucanase (Cel5A ; Ps_Cel5A; PST_2494)	3.2.1.4	Stutzerimonas stutzeri A1501	ABP80146.1	A4VME5	4LX4[A,B,C,D] 1.56 6R2J[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp 1.44	5
endoglucanase (RBcel1)	3.2.1.4	uncultured bacterium	ACO55737.1	C1JI15	4EE9[A] 1.38 4M24[A] β-D-Glcp-(1-4)-β-D-Glcp 1.39 5LJF[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.73 6ZZ3[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU 2.10 7P6G[A,B] 1.49 7P6H[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.73 7P6I[A] 1.47 7P6J[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU 1.75	5
XAC0029 (XacEgl5B)		Xanthomonas citri pv. citri str. 306	AAM34921.1 NP_640385.1 WP_011050033.1		5HOS[A] 1.60	5
XAC0030 (Egl)		Xanthomonas citri pv. citri str. 306	AAM34922.1 NP_640386.1		4W7U[A] 1.48 4W7V[A] β-D-Glcp-(1-4)-β-D-Glcp 1.43 4W7W[A] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.05 5HNN[A,B,C] 1.63 5HPC[A] 2.60	5
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)	Subf
endo-β-1,4-glucanase (Eng1; EgI; AnCel5A)	3.2.1.4	Aspergillus niger IFO31125	AAG50051.1	O74706 Q9C3Z7	5I77[A] β-D-GlcpNAc 1.80 5I78[A,B] 1.58 5I79[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 2.35	5
endo-β-1,4-glucanase (GlCel5A; GL28282) (Cel5A)	3.2.1.4	Ganoderma lucidum	QDK64599.1	A0A1A9TAJ1 A0A514YQS6	5D8W[A,B] 2.86 5D8Z[A,B] β-D-Glcp-(1-4)-β-D-Glcp 2.70	5
endoglucanase 2 (EG2;EGIIa) (partial) (Cel5A)	3.2.1.4	Talaromyces verruculosus B221-151		A0A1U7Q1U3	5L9C[A,B,C,D] β-D-Glcp-(1-4)-β-D-Glcp 1.85 6T9F[A,B,C,D] 2.25 6T9G[A,B,C,D] 2.30 6TPC[A,C] 1.52 6YON[A,B,C,D] 2.60 5I6S[A] 2.10	5
endo-β-1,4-glucanase (Eg1; Eng1; TaCel5A) (Cel5A)	3.2.1.4	Thermoascus aurantiacus IFO 9748	AAL88714.2 AAL16412.1 ACE10214.1 CAT02251.1 CAZ67882.1	Q8TG26 Q96UI5	1GZJ[A,B] 1.62 1H1N[A,B] 1.12 7R28[A,B] 1.22 7R29[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp-(1-4)-GLU 1.28 7R2A[A,B] 1.30 7R2C[A,B] β-D-Glcp-(1-1)-<non_carb> 1.48 7R2D[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.61	5
endo-β-1,4-glucanase II (EG2; EGII; Egl2 ;EG-II; TrEGII; TrCel5A) (formerly EGIII) (Cel5A)	3.2.1.4	Trichoderma reesei	AAA34213.1 AAQ24883.1 AAR60515.1 ABA64553.1 ABE22094.1	P07982 Q2F8H3	3QR3[A,B] 2.05	5
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