Mechanism	Retaining a
Clan	GH-T
3D Structure Status	( β / α ) 7 barrel
Catalytic Nucleophile/Base	Glu
Catalytic Proton Donor	Asp
Note	Subfamilies established in (Polacek et al., 2025) [PMID:40187459]
External resources	CAZypedia;
Statistics	GenBank accession (6112); Uniprot accession (40); PDB accession (32); 3D entries (8); cryst (0)

Archaea
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
4-α-glucanotransferase (formerly cyclodextrin glucanotransferase)	2.4.1.25	pubmed	Archaeoglobus fulgidus 7324 / DSM8774	ABW95829.1	A9QMB3		1
bifunctional amylopullulanase cyclomaltodextrinase (CMApu;Cmaq_1371)	3.2.1.1 3.2.1.41 3.2.1.54	doi doi doi	Caldivirga maquilingensis IC-167	ABW02197.1	A8M8X8		7
amylopullulanase (DApu)	3.2.1.1 3.2.1.41	pubmed pubmed	Desulfurococcus amylolyticus Z-533	WP_042667248.1			7
α-amylase (MJ1611)	3.2.1.1	pubmed	Methanocaldococcus jannaschii DSM 2661	AAB99631.1 NP_248621.1	Q59006		3
α-amylase (Pcal_0672)	3.2.1.1	doi	Pyrobaculum calidifontis JCM 11548	ABO08098.1			6
amylopullulanase (Pcal-apu; Pcal_1616)	3.2.1.1 3.2.1.41	URL URL	Pyrobaculum calidifontis JCM 11548	ABO09033.1	A3MWL6		2
α-amylase (AmyA; PaAD; PAB0118)	3.2.1.1	pubmed	Pyrococcus abyssi GE5	CAB49100.1 NP_125869.1	Q9V298		1
4-α-glucanotransferase (AmyA; PfuAmyGT; PF0272) (Amy57A)	2.4.1.25	pubmed	Pyrococcus furiosus DSM 3638	AAL80396.1 AAA72035.1 NP_578001.1	P49067		1
amylopullulanase (Apu; PFAPU; Apu-Pf; PF1935; PF1935*) (Amy57B)	3.2.1.1 3.2.1.41	pubmed pubmed	Pyrococcus furiosus DSM 3638	AAL82059.1 AAB71229.1 ABA33719.1 NP_579664.1	O30772 Q3HUR3 Q8TZQ1		2
maltogenic α-amylase (PF0870)	3.2.1.133	pubmed	Pyrococcus furiosus DSM 3638	AAL80994.1 NP_578599.1	Q8U2G5		8
α-galactosidase (GalA; PF0444) (Gal57)	3.2.1.22	doi	Pyrococcus furiosus DSM 3638	AAG28455.1 AAL80568.1 NP_578173.1	E7FHU9 Q9HHB5		4
glycogen branching enzyme (GBE;PhGBE;PH1386)	3.2.1.1 2.4.1.18	pubmed pubmed	Pyrococcus horikoshii OT3	BAA30492.1 NP_143265.1	O50094	5WU7[A,B]	5
4-α-glucanotransferase (PSGT; Py04_0423)	2.4.1.25	pubmed	Pyrococcus sp. ST04	AFK22025.1	I3RCR5		1
maltose-forming α-amylase (PSMA; Py04_0872)	3.2.1.133	pubmed	Pyrococcus sp. ST04	AFK22464.1	I3RE04	4CMR[A,B]	8
amylopullulanase (PulPY; PYCH_17120)	3.2.1.1 3.2.1.41	pubmed pubmed	Pyrococcus yayanosii CH1	AEH25371.1	F8AHK0		2
α-amylase (AmyA; SSO1172)	3.2.1.1	pubmed	Saccharolobus solfataricus P2	AAK41420.1 NP_342630.1	Q97YY0		7
bifunctional amylopullulanase cyclomaltodextrinase (Apu-Sm; SMApu; Smar_1407) (intracellular)	3.2.1.1 3.2.1.41 3.2.1.54	pubmed pubmed pubmed	Staphylothermus marinus F1	ABN70497.1	A3DPD7	7E1Y[A,B,C,D,E,F,G,H]	7
amylopullulanase (AmyA;Saci_1162)	3.2.1.1 3.2.1.41	pubmed pubmed	Sulfolobus acidocaldarius DSM 639	AAY80509.1	Q4J9M2		7
dual α-amylase / glycogen-branching enzyme (AmyA; Saci_1200)	3.2.1.1 2.4.1.18	pubmed pubmed	Sulfolobus acidocaldarius DSM 639	AAY80547.1			3
maltose-forming α-amylase (TCMA; CL1_0868)	3.2.1.133	pubmed	Thermococcus cleftensis	AFL95073.1	I3ZTN9		8
amylopullulanase (ApuA)	3.2.1.1 3.2.1.41	pubmed pubmed	Thermococcus hydrothermalis AL662	AAD28552.1	Q9Y8I8		2
amylopullulanase (Apu-Tk; TK1774)	3.2.1.1 3.2.1.41	doi doi	Thermococcus kodakarensis KOD1	BAD85963.1 CAT69104.1	Q5JJ55		2
branching enzyme (TkGBE; TkGBE57; TkGBE57; TK1436)	2.4.1.18	pubmed	Thermococcus kodakarensis KOD1	BAD85625.1 CAT71088.1 WP_011250387.1	Q5JDJ7	3N8T[A] 3N92[A] 3N98[A]	5
4-α-glucanotransferase (GtpK; TK1809)	2.4.1.25	doi	Thermococcus kodakarensis KOD1	BAA22062.1 BAD85998.1 CAT70994.1	O32450		1
amylopullulanase (Apu; TLAPU)	3.2.1.1 3.2.1.41	pubmed pubmed	Thermococcus litoralis	BAC10983.1	Q8NKS8		2
4-α-glucanotransferase (Jgt)	2.4.1.25	pubmed	Thermococcus litoralis	BAA22063.1	O32462	1K1W[A] 1K1X[A,B] 1K1Y[A,B]	1
4-α-glucanotransferase / amylomaltase (TonAmyGT; TON_1716)	2.4.1.25	pubmed	Thermococcus onnurineus NA1	ACJ17206.1	B6YUX8		1
amylopullulanase (TsiApu; Apu-Ts)	3.2.1.1 3.2.1.41	pubmed pubmed	Thermococcus sp. HJ21	ACJ03924.1	B6SED6		2
amylopullulanase (TPApu; Tpen_0210)	3.2.1.1 3.2.1.41	pubmed pubmed	Thermofilum pendens Hrk 5	ABL77620.1	A1RWP0		7
Bacteria
Protein Name	EC#	Reference	Organism	GenBank	Uniprot	PDB/3D	Subf
amylopullulanase (AaApu; APU_Aquae; Aq_720)	3.2.1.1 3.2.1.41	doi doi	Aquifex aeolicus VF5	AAC06900.1 NP_213494.1 WP_010880432.1	O66934	8ZYI[A,B] 9IHT[A,B] 9IHU[A,B] 9IHV[A,B] 9IHW[A,B] 9IHX[A,B] 9II0[A,B] 9II1[A,B] 9JLQ[A,B,C,D] 9JLR[A,B,C,D] 9JLS[A,B,C,D] 9JLT[A,B] 9JLU[A] 9JLV[A] 9JLW[A,B] 9K7C[A,B,C,D] 9K7D[A,B] 9KYP[A,B,C,D] 9KYQ[A,B] 9KYR[A,B,C,D]	2
amylopullulanase (Apu; CEApu; CSE_07380)	3.2.1.1 3.2.1.41	doi doi	Caldisericum exile AZM16c01	BAL80864.1	A0A7U6GEE7		2
4-α-glucanotransferase / α-amylase A (AmyA)	2.4.1.25	pubmed	Dictyoglomus thermophilum	CAA30735.1	P09961		1
pullulanase (Dtur_0675)	3.2.1.41	pubmed	Dictyoglomus turgidum DSM 6724	ACK41960.1	B8DZM7		2
α-1,4-glucan branching enzyme (MsGBE57; EWH23_01975)	2.4.1.18	pubmed	Meiothermus sp. PNK-Is4	RYM39963.1 WP_129865543.1			5
α-1,4-glucan branching enzyme (GlgB57; PmGBE57; Pmob_0588)	2.4.1.18	pubmed	Petrotoga mobilis SJ95	ABX31322.1	A9BH92		5
α-1,4-glucanotransferase / amylomaltase (PoGH57; C1N53_10890)	3.2.1.1 2.4.1.25	pubmed pubmed	Pontibacter sp. SGAir0037	QCR22797.1	A0A4P8RDB5		6
α-amylase (Amo45; Thal_1401)	3.2.1.1	pubmed	Thermocrinis albus DSM 14484	ADC90030.1 YP_003474157.1	D3SMQ0		6
α-1,4-glucan branching enzyme / α-amylase (AmyC; GBE; TmGBE57; TM1438)	3.2.1.1 2.4.1.18	pubmed pubmed	Thermotoga maritima MSB8	AHD18669.1 AGL50368.1 AKE29197.1 QFD27285.1 WP_004081707.1	R4P360	2B5D[X]	5
glycogen branching enzyme (GBE; TtGBE; TtGBE57; TTHA1902; TT1467)	2.4.1.18	pubmed	Thermus thermophilus HB8	BAD71725.1	Q5SH28	1UFA[A] 3P0B[A]	5
α-amylase (Amy_GH57)	3.2.1.1	pubmed	uncultured bacterium	AEC23345.1	F4ZW71		6
α-amylase (Amy2; persiAmy2)	3.2.1.1	doi	uncultured bacterium	QJT73070.1	A0A7D3P4A5		3
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