Glycoside Hydrolase Family 57

Mechanism Retaining a
ClanGH-T
3D Structure Status( β / α ) 7 barrel
Catalytic Nucleophile/BaseGlu
Catalytic Proton DonorAsp
NoteSubfamilies established in (Polacek et al., 2025) [PMID:40187459]
External resourcesCAZypedia;
Statistics GenBank accession (6112); Uniprot accession (40); PDB accession (32); 3D entries (8); cryst (0)
Archaea
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 glycogen branching enzyme (GBE;PhGBE;PH1386) 3.2.1.1
2.4.1.18
Pyrococcus horikoshii OT3 BAA30492.1
NP_143265.1
O50094
5WU7[A,B] 2.31
5
 maltose-forming α-amylase (PSMA; Py04_0872) 3.2.1.133 Pyrococcus sp. ST04 AFK22464.1 I3RE04
4CMR[A,B] 1.80
8
 bifunctional amylopullulanase cyclomaltodextrinase (Apu-Sm; SMApu; Smar_1407) (intracellular) 3.2.1.1
3.2.1.41
3.2.1.54
Staphylothermus marinus F1 ABN70497.1 A3DPD7
7E1Y[A,B,C,D,E,F,G,H] 2.90
7
 branching enzyme (TkGBE; TkGBE57; TkGBE57; TK1436) 2.4.1.18 Thermococcus kodakarensis KOD1 BAD85625.1
CAT71088.1
WP_011250387.1
Q5JDJ7
3N8T[A] 2.40
3N92[A] β-D-Glcp
2.89
3N98[A] β-D-Glcp
1.87
5
 4-α-glucanotransferase (Jgt) 2.4.1.25 Thermococcus litoralis BAA22063.1 O32462
1K1W[A] α-D-Glcp
2.80
1K1X[A,B] 2.40
1K1Y[A,B] α-D-Glcp-(1-4)-α-D-Glcp
<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp
2.40
1
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
Subf
 amylopullulanase (AaApu; APU_Aquae; Aq_720) 3.2.1.1
3.2.1.41
Aquifex aeolicus VF5 AAC06900.1
NP_213494.1
WP_010880432.1
O66934
8ZYI[A,B]
9IHT[A,B]
9IHU[A,B]
9IHV[A,B]
9IHW[A,B]
9IHX[A,B]
9II0[A,B]
9II1[A,B]
9JLQ[A,B,C,D]
9JLR[A,B,C,D]
9JLS[A,B,C,D]
9JLT[A,B]
9JLU[A]
9JLV[A]
9JLW[A,B]
9K7C[A,B,C,D]
9K7D[A,B]
9KYP[A,B,C,D]
9KYQ[A,B]
9KYR[A,B,C,D]
2
 α-1,4-glucan branching enzyme / α-amylase (AmyC; GBE; TmGBE57; TM1438) 3.2.1.1
2.4.1.18
Thermotoga maritima MSB8 AHD18669.1
AGL50368.1
AKE29197.1
QFD27285.1
WP_004081707.1
R4P360
2B5D[X] 2.20
5
 glycogen branching enzyme (GBE; TtGBE; TtGBE57; TTHA1902; TT1467) 2.4.1.18 Thermus thermophilus HB8 BAD71725.1 Q5SH28
1UFA[A] 2.20
3P0B[A] 1.35
5
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