| Mechanism | Retaining a |
|---|---|
| Clan | GH-H |
| 3D Structure Status | ( β / α ) 8 barrel |
| Catalytic Nucleophile/Base | Asp (experimental) |
| Catalytic Proton Donor | Glu (experimental) |
| Note | New: many members have been assigned to subfamilies as described by Stam et al. (2006) Protein Eng Des Sel. 19, 555-562 (PMID: 17085431) |
| External resources | CAZypedia; EBI Protein of the Month; HOMSTRAD; PDB Molecule of the Month; PRINTS; |
| Commercial Enzyme Provider(s) | MEGAZYME; PROZOMIX; |
| Statistics | GenBank accession (1107); Uniprot accession (42); PDB accession (36); 3D entries (14); cryst (0) |
| Bacteria | ||||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |||
| α-amylase (AliC) | 3.2.1.1 | pubmed |
Alicyclobacillus sp. 18711 | AWX66236.1 | A0A3P8MUS3 | 6GXV[A,B] 6GYA[A,B,C,D] |
5 | |||
| α-amylase (Amy1;AmyA;AmyB) | 3.2.1.1 | pubmed |
Alkalimonas amylolytica N10 | AAQ01675.1 | Q6WUB6 | 8XC9[A,B] | 5 | |||
| α-amylase (AFA;AMY1048) | 3.2.1.1 | doi |
Anoxybacillus flavithermus | CAL14744.1 | 5 | |||||
| α-amylase (Amy1;AmyQ;BAA) | 3.2.1.1 | pubmed |
Bacillus amyloliquefaciens | AAA22191.1 AAE12381.1 AAE13947.1 AAE37101.1 AAE59727.1 AAE62349.1 AAN20003.1 AAN27445.1 AAN99911.1 AAP02211.1 AAR63623.1 AAR76030.1 AAS26291.1 AAY72173.1 ABJ43095.1 ABJ43099.1 ABN29369.1 ACK07344.1 ACP75069.1 ACS10035.1 ACW26904.1 CAA01489.1 CAA23430.1 CAC16484.1 CAC88551.1 CAD19035.1 CAD20678.1 CAD26702.1 CAD67517.1 CAD88112.1 |
P00692 | 3BH4[A,B] | 5 | |||
| α-amylase | 3.2.1.1 | doi |
Bacillus amyloliquefaciens ATCC 23842 | ADE44086.1 | D5KR60 | 5 | ||||
| α-amylase (BCA) | 3.2.1.1 | pubmed |
Bacillus cereus GUF8 | ABY86223.1 | B0LVG1 | 5 | ||||
| α-amylase (Am-Lich) | 3.2.1.1 | Bacillus licheniformis | AAE12380.1 | 5 | ||||||
| α-amylase | 3.2.1.1 | Bacillus licheniformis | AAE12379.1 ABA38472.1 ACS10033.1 |
5 | ||||||
| α-amylase (AmyL) | 3.2.1.1 | Bacillus licheniformis 44MB82/G | AGR34077.1 | Q208A7 | 5 | |||||
| α-amylase (AmyA;AmyL;AmyS;BLA) | 3.2.1.1 | pubmed |
Bacillus licheniformis 584 / ATCC 27811 | AAA22226.1 AAA22193.1 AAA22240.1 AAN99913.1 AAO26743.1 AAT46561.1 ACM17155.1 CAA01355.1 CAA03075.1 CAA26981.1 CAC16483.1 CAC88550.1 CAD19034.1 CAD20677.1 CAD26701.1 CAD26706.1 CAD88111.1 CAV31708.1 |
P06278 Q208A7 Q6GWE2 |
1BLI[A] 1BPL[A,B] 1OB0[A] 1VJS[A] |
5 | |||
| amylase (BliAmy) | 3.2.1.1 | doi |
Bacillus licheniformis ATCC 9945a | AEM05860.1 | I3P686 | 6TOY[A] 6TOZ[A] 6TP0[A] 6TP1[A] 6TP2[A] |
5 | |||
| α-amylase (AmyL) | 3.2.1.1 | Bacillus licheniformis B0204 | ABD66592.1 | Q208A7 | 5 | |||||
| α-amylase (thermostable) | 3.2.1.1 | Bacillus licheniformis CICC 10181 | ACN88150.1 | C0LZX2 | 5 | |||||
| α-amylase (Amy;BLi00656;BL00499) | 3.2.1.1 | Bacillus licheniformis DSM 13 = ATCC 14580 | AAU39594.1 AAU22245.1 |
Q65MX0 | 5 | |||||
| α-amylase (AmyB) | 3.2.1.1 | Bacillus licheniformis MD1 | CAH10338.1 | P06278 Q6GWE2 |
5 | |||||
| α-amylase | 3.2.1.1 | Bacillus licheniformis NCIB8061 | AAE12384.1 | 5 | ||||||
| α-amylase (AmyN) | 3.2.1.1 | Bacillus licheniformis NH1 | ABL75259.1 | A1YR25 | 5 | |||||
| α-amylase (AmyL;BaLi_c07450) | 3.2.1.1 | pubmed |
Bacillus paralicheniformis ATCC 9945a | AGN35141.1 | 5 | |||||
| alkaline α-amylase (AmyK) | 3.2.1.1 | Bacillus sp. (in: firmicutes) KSM-1378 | AAR68734.1 AAN99906.1 AAR63625.1 AAV01045.1 ABE15847.1 ABI05897.1 ABY03536.1 ACS10038.1 ACW26917.1 BAA32431.1 CAD35985.1 CAD38135.1 CAV31709.1 |
O82839 | 2DIE[A] | 5 | ||||
| maltohexaose-forming amylase | 3.2.1.98 | pubmed |
Bacillus sp. 707 | AAA22231.1 AAE59728.1 AAE62350.1 AAP02212.1 AAR63620.1 AAS26292.1 AAY06019.1 AAY72174.1 ACK07346.1 ACP75071.1 ACS10032.1 ACW26905.1 |
P19571 | 1WP6[A] 1WPC[A] 2D3L[A] 2D3N[A] |
5 | |||
| maltopentaose-producing α-amylase (Amy204) | 3.2.1.- | Bacillus sp. JAMB-204 | BAF03567.1 | Q0KKZ7 | 5 | |||||
| α-amylase (PRK09441) (fragment) | 3.2.1.1 | Bacillus sp. KR-8104 | AAW29920.1 | Q5MB94 | 5 | |||||
| α-amylase (AmyK38) | 3.2.1.1 | doi |
Bacillus sp. KSM-K38 | CAC39917.1 AAN18958.1 AAV01046.1 ABA16539.1 ABI05896.1 ABY03537.1 ACS10039.1 BAB71820.1 CAD35986.1 CAD38133.1 CAJ00040.1 CAJ28078.1 |
Q93I48 | 1UD2[A] 1UD3[A] 1UD4[A] 1UD5[A] 1UD6[A] 1UD8[A] 9FZ3[A] |
5 | |||
| α-amylase (Amy) | 3.2.1.1 | pubmed |
Bacillus sp. TS-23 | AAA63900.1 | Q59222 | 5 | ||||
| α-amylase (Amy;BYXA) | 3.2.1.1 | Bacillus sp. YX / YX-1 | ABW87262.1 | A9YDD9 | 5 | |||||
| α-amylase (RSDA) (raw-starch-digesting) | 3.2.1.1 | Cytophaga sp. | AAF00567.1 | Q9RQT8 | 5 | |||||
| α-amylase 2 (AmyAl;EAMY) | 3.2.1.1 | pubmed |
Escherichia coli str. K-12 substr. MG1655 | AAC74994.1 AAA23810.1 AAA82575.1 AIZ90980.1 ALI40306.1 AMC94904.1 AUG16689.1 AVI56440.1 AYG19026.1 NP_416437.1 |
P26612 | 5 | ||||
| α-amylase (AmyH) | 3.2.1.1 | pubmed |
Exiguobacterium sp. DAU5 | AFZ41193.1 | M4I6P6 | 5 | ||||
| α-amylase (Am-Stearo) | 3.2.1.1 | Geobacillus stearothermophilus | AAE12382.1 | 5 | ||||||
| α-amylase (Ami;Amy;AmyUS100) | 3.2.1.1 | pubmed |
Geobacillus stearothermophilus US100 / ATCC 31195 | AAB86961.1 AAN99912.1 ACH26104.1 CAB93517.1 CCB84710.1 |
G0LY26 O31193 Q9KWY6 |
5 | ||||
| α-amylase (AmyS) | 3.2.1.1 | pubmed |
Geobacillus stearothermophilus DY5 / PHI300 / NZ-3 | AAA22235.2 AAA22227.1 AAA22241.1 AAE13948.1 AAE37102.1 AAE59725.1 AAE62347.1 AAN20004.1 AAN27446.1 AAP02209.1 AAR63624.1 AAS26289.1 AAY06018.1 AAY72171.1 ABJ43096.1 ABN29370.1 ACK07342.1 ACP75067.1 ACW26902.1 CAA26547.1 CAC16485.1 CAC88549.1 CAD19033.1 CAD20676.1 CAD26700.1 CAD67515.1 |
P06279 | 1HVX[A] 4UZU[A] |
5 | |||
| α-amylase | 3.2.1.1 | doi |
Geobacillus stearothermophilus GRE1 | ABC18196.1 | 5 | |||||
| α-amylase | 3.2.1.1 | Geobacillus stearothermophilus TISTR 329 | AAU10478.1 | Q66T78 | 5 | |||||
| α-amylase (GA) | 3.2.1.1 | Geobacillus thermodenitrificans LH8 | ABX83871.1 | A9UJ60 | 5 | |||||
| α-amylase (Amy;Gt-Amy) | 3.2.1.1 | Geobacillus thermoleovorans NP54 | AFC87833.1 | J9PXA2 | 5 | |||||
| α-amylase B (AmyB) | 3.2.1.1 | pubmed |
Halothermothrix orenii H 168 | ACL70573.1 ACB11224.1 |
B8CZ54 | 3BC9[A] 3BCD[A] 3BCF[A] |
5 | |||
| α-amylase (fragment) | 3.2.1.1 | pubmed |
Heyndrickxia acidicola TSAS1 | AEW07376.1 | J9PQD2 | 5 | ||||
| α-amylase (Amy1) | 3.2.1.1 | Nostoc sp. PCC 7119 | CAQ30277.1 | B1VK33 | 5 | |||||
| α-amylase (AmyZ1) | 3.2.1.1 | pubmed |
Pontibacillus sp. ZY | AXV43605.1 | A0A385FPX0 | 5 | ||||
| α-amylase II (Amy II) | 3.2.1.1 | Streptococcus equinus 148 | BAA24178.1 | O50583 | 5 | |||||
| α-amylase | 3.2.1.1 | Streptococcus equinus JB1 | AAA97431.1 | Q53786 | 5 | |||||
| α-amylase (intracellular) (Amy) | 3.2.1.1 | Streptococcus mutans LT11 | AAC35010.1 | O68875 | 5 | |||||
| α-amylase (AmyA;SMU.1590) | 3.2.1.1 | Streptococcus mutans UA159 | AAN59233.1 NP_721927.1 |
Q8DT08 | 5 | |||||
| α-amylase | 3.2.1.1 | pubmed |
Sutcliffiella halmapala | CAD26699.1 AAE12377.1 AAE59724.1 AAE59730.1 AAE62346.1 AAE62352.1 AAP02208.1 AAP02214.1 AAS26288.1 AAS26294.1 AAY06017.1 AAY72170.1 AAY72176.1 ACK07341.1 ACP75066.1 ACS10037.1 ACW26901.1 CAC88548.1 CAD19032.1 CAD20675.1 CAD67514.1 |
1W9X[A] 2GJP[A] 2GJR[A] |
5 | ||||
| α-amylase (Amy13A) | 3.2.1.1 | pubmed |
uncultured bacterium | CCD30600.1 | I7I8P3 | 5 | ||||
| α-amylase (Amy63) | 3.2.1.1 | Vibrio alginolyticus 129-63 | ALP73597.1 | A0A0S2UQL4 | 8JJM[A,B] | 5 | ||||
| Eukaryota | ||||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |||
| maltotriose-producing α-amylase (AmyD;An01g13610) (intracellular) | 3.2.1.1 3.2.1.116 |
Aspergillus niger CBS 513.88 | CAK37367.1 | A2QB23 | 5 | |||||
| cell-wall active enzyme / α-amylase (Amy1;PbrAmy1) | 3.2.1.1 | pubmed |
Paracoccidioides brasiliensis IVIC Pb73 / ATCC 3207 | ABS11196.1 | A7L832 | 5 | ||||
| α-amylase (NFAmy13B) | 3.2.1.1 | pubmed |
uncultured fungus | UIX27107.1 | 5 | |||||
| unclassified | ||||||||||
| Protein Name | EC# | Reference | Organism | GenBank | Uniprot | PDB/3D | Subf | |||
| α-amylase | 3.2.1.1 | pubmed |
uncultured organism | ADD61689.1 | D9ZED5 | 5 | ||||
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