Mechanism	Retaining e
Clan	GH-C
3D Structure Status	β-jelly roll
Catalytic Nucleophile/Base	Glu (experimental)
Catalytic Proton Donor	Glu (experimental)
Note	formerly known as cellulase family H.
External resources	CAZypedia; HOMSTRAD;
Commercial Enzyme Provider(s)	MEGAZYME;
Statistics	GenBank accession (3411); Uniprot accession (94); PDB accession (61); 3D entries (23); cryst (1)

Archaea
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-glucanase A (EglA; EGPf; TCel1; PF0854) (Cel12)	3.2.1.4	Pyrococcus furiosus DSM 3638	AAL80978.1 AAD54602.1 AAE50601.1 AAM56799.1 AAW12536.1 ABJ38334.1 ACM94275.1 ACW20099.1 AEG76943.1 NP_578583.1	E7FHY8 Q9V2T0	3VGI[A] 1.07 3WQ0[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.22 3WQ1[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp 1.30 3WQ7[A,B] 1.68 3WR0[A] 1.16 3WT3[A,B] 1.68 3WXP[A] β-D-Glcp-(1-4)-β-D-Glcp 1.42 3WY6[A] β-D-Glcp β-D-Glcp-(1-3)-β-D-Glcp 1.45
multidomain glycosidase (MDG; ADU37_CDS22600)	3.2.1.4	Thermococcus sp. 2319x1	ALV63957.1	A0A0U3SGP7	7S8K[A,B] 2.55
Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endoglucanase (GuxA; Acel_0615) (Cel6A-Cel12A)	3.2.1.4	Acidothermus cellulolyticus 11B	ABK52388.1	A0LSH8	7MKR[A] 7MKS[A]
endo-β-1,4-glucanase / xyloglucanase (XG12;BLi03304;BL02586) (Cel12A)	3.2.1.4 3.2.1.151	Bacillus licheniformis DSM 13 = ATCC 14580	AAU42138.1 AAU24774.1	Q65FM6 Q7X4S4	2JEM[A,B] 1.78 2JEN[A] α-D-Xylp-(1-6)-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp 1.4
cellulase A (CelA) (Cel12A)	3.2.1.4	Rhodothermus marinus ITI378	AAB65594.1 AAR63650.1 AAR67703.1 ABJ29234.1 ACP76253.1	O33897	1H0B[A,B] 1.8 2BW8[A,B] 1.54 2BWA[A,B] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.68 2BWC[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-1)-<non_carb> 2.15 3B7M[A,B,C,D] 2.10
xyloglucanase / type III effector (NopAA; GunA)	3.2.1.151	Sinorhizobium fredii USDA257	AGH09926.1	A0A0T6ZV48 M4PUR5	6SDU[A,B,C,D] β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp 2.20
endo-β-1,4-glucanase (CelB;CelB2;EGI)	3.2.1.4	Streptomyces lividans 1326	AAB71950.1 AAR63649.1 AAR67702.1 ABH87634.1 ABJ29233.1 ACP76252.1	Q54331	1NLR[A] 1.75 2NLR[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp2F 1.20
cellulase 12A (Cel12A)	3.2.1.4	Streptomyces sp. 11AG8	AAF91283.1 AAE59731.1 AAQ25310.1 AAQ31830.1 AAR63648.1 AAR67701.1 ACP76251.1	Q9KIH1	1OA4[A] 1.5
endo-β-1,4-glucanase A (CelA;TmCel12A;TM1524;TM_1524;Tmari_1532) (Cel12A)	3.2.1.4	Thermotoga maritima MSB8	AGL50456.1 AAD36591.1 AHD18579.1 AKE29285.1 CAA93273.1 NP_229324.1	G4FFR2 Q60032 Q9S5X8	3AMH[A,B] 2.09 3AMM[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.98 3AMN[A,B] β-D-Glcp-(1-4)-α-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp 1.47 3AMP[A,B] β-D-Glcp-(1-4)-β-D-Glcp β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-α-D-Glcp 1.78 3AMQ[A,B,C,D] β-D-Glcp β-D-Glcp-(1-4)-α-D-Glcp 1.80 3O7O[A,B] 2.41 3VHN[A,B,C,D,E,F,G,H] β-D-Glcp-(1-4)-β-D-Glcp 2.50 3VHO[A,B] 1.93 3VHP[A,B] β-D-Glcp-(1-4)-β-D-Glcp 1.93
cellulase (Lc-CelA)	3.2.1.4	uncultured bacterium	AHL27894.1	W8PWF3	3WX5[A,B] 1.85
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
endo-β-1,4-glucanase 1 (Cmc1;Cmc-1;FI CM-cellulase;FI-CMCase)	3.2.1.4	Aspergillus aculeatus F-50	BAA00435.1 AAR63632.1 AAR67685.1 ABJ29216.1 ACP76235.1 CAA36757.1	P22669	5GM3[A,B] 1.59 5GM4[A,B,C,D,E,F,G] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.92 5GM5[A,B,C,D,E,F,G] β-D-Glcp-(1-4)-β-D-Glcp 1.73
endo-β-1,4-glucanase (xyloglucan-specific) (XEG1)	3.2.1.151	Aspergillus aculeatus KSM 510	AAD02275.1	O94218	3VL8[A] 1.90 3VL9[A,B] α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp α-D-Xylp-(1-6)[β-D-Glcp-(1-4)]-β-D-Glcp-(1-4)[α-D-Xylp-(1-6)]-β-D-Glcp-(1-4)-β-D-Glcp 1.20 3VLB[B,D] 2.70
GH12 β-1, 4-endoglucanase		Aspergillus fischeri CGMCC 3.15369	APQ48493.1		6K98[A] 2.03 6K9D[A] 1.51
endo-β-1,4-glucanase (EglA; AnEglA)	3.2.1.4	Aspergillus niger CBS 120.49 / N400	AAE60101.1 AAE84291.1 CAA03652.1 CAA03657.1 CAA11964.1 CAG17891.1	O74705	1KS4[A] 2.50 1KS5[A] 2.10
xyloglucan-specific endo-β-1,4-glucanase (XegA)	3.2.1.151	Aspergillus niveus PR-2	AEV23011.1	H6TQN1	4NPR[A,B] 2.50
endoglucanase (EglD;AtEglD;AtGH12;ATEG_09894)	3.2.1.4	Aspergillus terreus NIH2624	EAU30085.1 XP_001218516.1	Q0C8U0	cryst
endo-β-1,3-1,4-glucanase (CtGlu12)	3.2.1.73	Chaetomium sp. CQ31		A0A915Q9D1	7EE2[A] 1.37 7EEE[A] β-D-Manp-(1-6)-β-D-Manp 1.66 7EEJ[A] β-D-Manp-(1-4)-β-D-Manp-(1-3)-β-D-Manp-(1-4)-β-D-Manp 1.48
Endoglucanase-like protein (ACRE_041060)		Hapsidospora chrysogenum ATCC 11550			5M2D[B] 2.10 5M2D[A] 2.10
endoglucanase (Cel12A)	3.2.1.4	Humicola grisea ATCC 22081	AAM77714.2 AAR63636.1 AAR63637.1 AAR67689.1 AAR67690.1 ABJ29220.1 ABJ29221.1 ACP76239.1 ACP76240.1	Q8NJY3	1OLR[A] 1.2 1UU4[A] β-D-Glcp-(1-4)-β-D-Glcp 1.49 1UU5[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-3)-β-D-Glcp-(1-4)-β-D-Glcp 1.7 1UU6[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.4 1W2U[A] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp 1.52
endoglucanase (Cel12A)	3.2.1.4	Hypocrea schweinitzii ATCC 66965	AAM77711.1 AAE59776.1 AAN19481.1 AAO95540.1 AAQ42102.1 AAR63631.1 AAR67684.1 ABJ29215.1 ACP76234.1	Q8NJY6	1OA3[A,B,C,D] 1.7
xyloglucan-specific endoglucanase (EGL12-G;XEG1;PsXEG1;Ps109681;PHYSODRAFT_559651) (Cel12G)	3.2.1.151	Phytophthora sojae	ABB22029.1 EGZ16757.1	G4ZHR2 Q30BZ2	7DRB[A,C] 7DRC[A] 2.92 7W3V[A] 3.11
endo-β-1,4-glucanase 3 / III (Egl3;EG3;EGIII;ThEG3;ThEGIII) (Cel12A)	3.2.1.4	Trichoderma harzianum IOC-3844	AFK32784.1	I3RY46	4H7M[A,B] 2.07
endo-β-1,4-glucanase III (Egl3;EGIII;EG III;EG3;TrCel12A) (Cel12A)	3.2.1.4	Trichoderma reesei QM9414	AAE59774.1 AAE59775.1 AAN19479.1 AAN19480.1 AAO95538.1 AAO95539.1 AAQ24888.1 AAQ42100.1 AAQ42101.1 AAR60524.1 AAR63629.1 AAR63630.1 AAR67682.1 AAR67683.1 ABE22099.1 ABJ29213.1 ABJ29214.1 ABV71388.1 ACP76232.1 ACP76233.1 BAA20140.1	O00095	1H8V[A,B,C,D,E,F] 1.9 1OA2[A,B,C,D,E,F] 1.5 1OLQ[A,B] 1.7
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