Activities in Family	L-rhamnose-α-1,4-D-glucuronate lyase (EC 4.2.2.28);
Mechanism	b-elimination
3D Structure Status	7-fold β-propeller
Note	Formerly known as glycoside hydrolase family GH145 (Munoz-Munoz et al., PMID=28396425); subsequently reclassified as PL42 based on the publications of Kondo T et al. JBC 2021 (PMID=34303708) and Munoz-Munoz et al., mBio 2021 (PMID=34340552) showing lyase activity on aLRha-1,4-aDGlcA. Several members of the family show hydrolase activity catalyzed by a non-invariant active site on the posterior side of the b-propeller structure while the lyase active site on the anterior surface is well conserved and shows similarities to PL24 and PL25.
External resources	CAZypedia;
Statistics	GenBank accession (757); Uniprot accession (5); PDB accession (11); 3D entries (5); cryst (0)

Bacteria
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856)	4.2.2.28	Bacteroides cellulosilyticus DSM 14838	EEF91490.1	E2N9B1	5MVH[A] 1.80
BACINT_00347		Bacteroides intestinalis DSM 17393			5MUM[A] 1.80
Bovatus_03783 / BACOVA_03493		Bacteroides ovatus ATCC 8483	ALJ48388.1	A7M066	4IRT[A] 1.74
L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686; BT_3686)	4.2.2.28	Bacteroides thetaiotaomicron VPI-5482	AAO78791.1	Q8A1H5	5MUK[A] 1.49 5MUL[A] β-D-GlcpA 1.39
Eukaryota
Protein Name	EC#	Organism	GenBank	Uniprot	PDB/3D Carbohydrate Ligands Resolution (Å)
L-rhamnose-α-1,4-D-glucuronate lyase (Forham1)	4.2.2.28	Fusarium oxysporum 12S	BCT36655.1	A0A8D5PZ14	7ESK[A] 1.05 7ESL[A] 1.40 7ESM[A] α-L-Rhap 1.40 7ESN[A] α-L-Rhap-(1-4)-β-D-GlcpA 2.42 7YQS[A] α-L-Rhap 1.25 8I4D[A] α-L-Rhap 1.06
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