Polysaccharide Lyase Family 42

Activities in FamilyL-rhamnose-α-1,4-D-glucuronate lyase (EC;
Mechanism b-elimination
3D Structure Status7-fold β-propeller
NoteFormerly known as glycoside hydrolase family GH145 (Munoz-Munoz et al., PMID=28396425); subsequently reclassified as PL42 based on the publications of Kondo T et al. JBC 2021 (PMID=34303708) and Munoz-Munoz et al., mBio 2021 (PMID=34340552) showing lyase activity on aLRha-1,4-aDGlcA. Several members of the family show hydrolase activity catalyzed by a non-invariant active site on the posterior side of the b-propeller structure while the lyase active site on the anterior surface is well conserved and shows similarities to PL24 and PL25.
External resourcesCAZypedia;
Statistics GenBank accession (781); Uniprot accession (5); PDB accession (11); 3D entries (5); cryst (0)
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) Bacteroides cellulosilyticus DSM 14838 EEF91490.1 E2N9B1
5MVH[A] 1.80
 BACINT_00347   Bacteroides intestinalis DSM 17393    
5MUM[A] 1.80
 Bovatus_03783 / BACOVA_03493   Bacteroides ovatus ATCC 8483 ALJ48388.1 A7M066
4IRT[A] 1.74
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686; BT_3686) Bacteroides thetaiotaomicron VPI-5482 AAO78791.1 Q8A1H5
5MUK[A] 1.49
5MUL[A] β-D-GlcpA
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 L-rhamnose-α-1,4-D-glucuronate lyase (Forham1) Fusarium oxysporum 12S BCT36655.1 A0A8D5PZ14
7ESK[A] 1.05
7ESL[A] 1.40
7ESM[A] α-L-Rhap
7ESN[A] α-L-Rhap-(1-4)-β-D-GlcpA
7YQS[A] α-L-Rhap
8I4D[A] α-L-Rhap

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