Polysaccharide Lyase Family 42

Activities in FamilyL-rhamnose-α-1,4-D-glucuronate lyase (EC 4.2.2.-); L-Rhα-α-1,4-GlcA α-L-rhamnohydrolase (EC 3.2.1.-)
Mechanism b-elimination
3D Structure Status7-fold β-propeller
NoteFormerly known as glycoside hydrolase family GH145 (Munoz-Munoz et al., PMID=28396425); subsequently reclassified as PL42 based on the publications of Kondo T et al. JBC 2021 (PMID=34303708) and Munoz-Munoz et al., mBio 2021 (PMID=34340552) showing lyase activity on aLRha-1,4-aDGlcA. Several members of the family show hydrolase activity catalyzed by a non-invariant active site on the posterior side of the b-propeller structure while the lyase active site on the anterior surface is well conserved and shows similarities to PL24 and PL25.
External resourcesCAZypedia;
Statistics GenBank accession (486); Uniprot accession (4); PDB accession (9); 3D entries (5); cryst (0)
Bacteria
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (BACCELL_00856) 4.2.2.- pubmed
Bacteroides cellulosilyticus DSM 14838 EEF91490.1 E2N9B1 5MVH[A]
 Bovatus_03783 / BACOVA_03493 3.2.1.- pubmed
Bacteroides ovatus ATCC 8483 ALJ48388.1 A7M066 4IRT[A]
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase / exo-acting α-L-rhamnosidase (BT3686;BT_3686) 4.2.2.-
3.2.1.-
pubmed
pubmed
Bacteroides thetaiotaomicron VPI-5482 AAO78791.1 Q8A1H5 5MUK[A]
5MUL[A]
 L-Rhα-a-1,4-GlcA α-L-rhamnohydrolase (HMPREF9455_02360) 3.2.1.- pubmed
Dysgonomonas gadei ATCC BAA-286 EGK01332.1 F5IZ43  
Eukaryota
Protein Name EC#ReferenceOrganismGenBank UniprotPDB/3D
 L-rhamnose-α-1,4-D-glucuronate lyase (Forham1) 4.2.2.- pubmed
Fusarium oxysporum 12S BCT36655.1   7ESK[A]
7ESL[A]
7ESM[A]
7ESN[A]
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