Glycoside Hydrolase Family 97

Activities in Family	α-glucosidase (EC 3.2.1.20); α-galactosidase (EC 3.2.1.22); [inverting] glucoamylase (EC 3.2.1.3); Glucodextranase (EC 3.2.1.70); [retaining] β-L-arabinopyranosidase / β-L-arabinosidase (EC 3.2.1.88);
Mechanism	Inverting and retaining a
3D Structure Status	( β / α ) ₈ barrel
Catalytic Nucleophile/Base	Glu for inverting members; Asp for retaining members
Catalytic Proton Donor	Glu
Note	Created based on a paper by Hughes et al. (2003) Oral Microbiol Immunol. 18:309-312 (PMID: 12930523); *Mechanistic and structural elucidation by Gloster et al. (2008) Chem. Biol., Oct 8 (PMID:18848471); the inverting a-glucosidases (EC 3.2.1.20) could be renamed glucoamylases (EC 3.2.1.3 according to Kitamura et al. (2008) J. Biol. Chem., Nov 3 (PMID:18981178)
External resources	CAZypedia;
Statistics	GenBank accession (6048); Uniprot accession (13); PDB accession (16); 3D entries (5); cryst (0)

Bacteria

Protein Name

EC#

Organism

GenBank

Uniprot

PDB/3D

Carbohydrate Ligands

Resolution (Å)

α-galactosidase (BtGH97b; BT1871; BT_1871)

AAO76978.1
NP_810784.1

3A24[A,B]		2.30
5E1Q[A,B]		1.94

bifunctional β-L-arabinopyranosidase/α-D-galactosidase (BT3661)

AAO78766.1
NP_812572.1

2.30

α-glucosidase / glucoamylase (SusB; BtGH97a; BT3703; BT_3703)

AAO78808.1
AAC44671.1
NP_812614.1

2D73[A,B]		1.60
2JKA[A,B]		1.90
2JKE[A,B]	<carb_like_NOJ>	1.7
2JKP[A,B]	<carb_like_CTS>	1.99
2ZQ0[A,B]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp	1.60
3WFA[A,B]		2.00

dextran exo-α-1,6-glucosidase (FjGH97A; Fjoh_4429)

8WG0[A,B,C,D]	α-D-Glcp	1.95
8WG1[A,B,C,D]	α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-β-D-Glcp	2.34
8WG2[A,B,C,D]	α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-α-D-Glcp α-D-Glcp-(1-6)-α-D-Glcp-(1-6)-β-D-Glcp	2.45

dextran exo-α-1,6-glucosidase (PspAG97A) (Ag97A)

5HQ4[A]		1.93
5HQA[A]	<non_carb>-(1-4)-α-D-6-deoxy-Glcp4N-(1-4)-α-D-Glcp-(1-4)-α-D-Glcp	1.75
5HQB[A]	α-D-Glcp-(1-6)-α-D-Glcp-(1-4)-D-1-deoxy-Glcp	1.80
5HQC[A]		2.00