Glycoside Hydrolase Family 94

Activities in Familycellobiose phosphorylase (EC; laminaribiose phosphorylase (EC; cellodextrin phosphorylase (EC; chitobiose phosphorylase (EC 2.4.1.-); cyclic β-1,2-glucan synthase (EC 2.4.1.-); cellobionic acid phosphorylase (EC; β-1,2-oligoglucan phosphorylase (EC 2.4.1.-)
Mechanism Inverting
3D Structure Status( α / α ) 6
Catalytic Nucleophile/Basephosphate
Catalytic Proton DonorAsp
NoteFormerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L.
External resourcesCAZypedia;
Statistics GenBank accession (2578); Uniprot accession (337); PDB accession (31); 3D entries (8); cryst (0)
All (2517) Archaea (11) Bacteria (2479) Eukaryota (8) unclassified (19) Structure (8) Characterized (28)
Protein Name EC#OrganismGenBank UniprotPDB/3D
 Cellobiose phosphorylase (EC 2.4.1.-)   feces metagenome SJX74208.1    
 BN138_195 (fragment)   termite gut metagenome CCO21007.1    
 ORF   uncultured organism ADD61402.1
 ORF   uncultured organism ADD61963.1 D9ZF59  
 E1750_12290   Flavobacterium sp. GS13 QBN19544.1    
 W01_22870 (NdvB)   Candidatus Nitrotoga sp. AM1 BBJ24360.1    
 DAIF1_21780 (NdvB)   Stenotrophomonas sp. DAIF1 QBR44613.1    
 E4191_15545   Paracoccus sp. 2251 QBX35938.1    
 E4Z61_06160   Citrobacter sp. SNU WT2 QBX79973.1    
 E2K93_02180   Thalassotalea sp. HSM 34 QBY03248.1    
 E5678_04940   Hydrogenophaga sp. PAMC20947 QCB48586.1    
 E5678_16275   Hydrogenophaga sp. PAMC20947 QCB48774.1    
 E5225_16190   Cellulomonas sp. Z28 QCB94869.1    
 E7745_05145   Duncaniella sp. C9 QCD38979.1    
 FCN45_22560   Pantoea sp. SO10 QCP62173.1    
 FDZ78_08850   Duncaniella sp. B8 QCP72669.1    
 C1N80_02555   Brachybacterium sp. SGAir0954 QCR52578.1    
 E6C60_1090   Paenibacillus sp. HB172198 QCT01808.1    
 FE005_02740   Enterococcus sp. M190262 QCT90941.1    

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