Glycoside Hydrolase Family 94

Known Activitiescellobiose phosphorylase (EC 2.4.1.20); cellodextrin phosphorylase (EC 2.4.1.49); chitobiose phosphorylase (EC 2.4.1.-); cyclic β-1,2-glucan synthase (EC 2.4.1.-)
Mechanism Inverting
3D Structure Status( α / α ) 6
Catalytic Nucleophile/Basephosphate
Catalytic Proton DonorAsp
NoteFormerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L.
External resourcesCAZypedia;
Statistics GenBank accession (530); Uniprot accession (325); PDB accession (12); 3D entries (4); cryst (1)
All (497) Archaea (5) Bacteria (484) Eukaryota (6) unclassified (2) Structure (4 - 1 cryst) Characterized (19)
Bacteria
Protein Name EC#OrganismGenBankUniprotPDB/3D
 cyclic β-1,2-glucan synthetase (ChvB) 2.4.1.-
2.4.1.-
Agrobacterium tumefaciens str. C58 AAK73356.1 Q93N05  
 cyclic β-1,2-glucan synthetase (Cgs) 2.4.1.- Brucella abortus S19 AAC34747.1
AAC34747.2
ACD71661.1
B2S8A8
B2S8A8
O85166
 
 cellobiose phosphorylase (CbP) 2.4.1.20 Cellulomonas uda DSM 20108 AAQ20920.1 Q7WTR6 cryst
 cellodextrin-phosphorylase (CepB) 2.4.1.49 Clostridium stercorarium DSM8532 AAC45511.1 P77846  
 cellobiose-phosphorylase (CepA) 2.4.1.20 Clostridium stercorarium NCIB 11754 AAC45510.1 Q59316  
 cellodextrin-phosphorylase (Cdp;Cthe_2989) 2.4.1.49 Clostridium thermocellum ATCC 27405 ABN54185.1
BAA22081.1
A3DJQ6
O24780
 
 cellobiose phosphorylase (Cbp;Cthe_0275) 2.4.1.20 Clostridium thermocellum ATCC 27405 ABN51514.1 A3DC35  
 cellodextrin phosphorylase (Cdp-ym4) 2.4.1.49 Clostridium thermocellum YM4 BAB71818.1 Q93HT8  
 cellobiose phosphorylase (Cbp) 2.4.1.20 Clostridium thermocellum YM4 AAL67138.1 Q8VP44 3QDE[A,B]
 cellobiose-phosphorylase (Cbp) 2.4.1.20 Clostridium thermocellum YM4 BAA25846.1 O66383  
 cellobiose phosphorylase 94B (Sde_0906) (Cep94B) 2.4.1.20 Saccharophagus degradans 2-40 ABD80168.1 Q21MB1  
 cellobiose phosphorylase 94A (Sde_1318) (Cep94A) 2.4.1.20 Saccharophagus degradans 2-40 ABD80580.1 Q21L49  
 cyclic β-1,2-glucan synthase (NdvB;SMc04382) 2.4.1.- Sinorhizobium meliloti 1021 AAA26305.1
CAC47865.1
NP_387392.1
P20471.2  
 cellobiose-phosphorylase (CepA;TM1848) 2.4.1.20 Thermotoga maritima MSB8 AAD36910.1
NP_229644.1
Q9X2G3  
 cellobiose-phosphorylase (CepA) 2.4.1.20 Thermotoga neapolitana CAB16926.1 O87964  
 cellobiose-phosphorylase (CbpA) 2.4.1.20 Thermotoga neapolitana AAB95491.2 O52504  
 diacetylchitobiose phosphorylase (ChbP) 2.4.1.- Vibrio furnissii AAG23740.1 Q9F8X1  
 chitobiose phosphorylase (ChbP) 2.4.1.- Vibrio proteolyticus BAC87867.1 Q76IQ9 1V7V[A]
1V7W[A]
1V7X[A]
 cellobiose phosphorylase 2.4.1.20 [Cellvibrio] gilvus ATCC13127 BAA28631.1 O66264 2CQS[A,B]
2CQT[A,B]
3ACS[A,B]
3ACT[A,B]
3AFJ[A,B]
3QFY[A,B]
3QFZ[A,B]
3QG0[A,B]

Footnotes

Experimental characterization exclusively covers enzyme activities at present. If you are aware of any missing experimental characterization that should be mentioned here, we encourage you to send your comment and support of the experimental evidence, including an electronic copy or appropriate link to the support material (i.e., article(s), thesis, database, etc), to cazy@afmb.univ-mrs.fr.





Last update: 2012-01-31 © Copyright 1998-2012
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