Glycoside Hydrolase Family 94

Activities in Familycellobiose phosphorylase (EC 2.4.1.20); laminaribiose phosphorylase (EC 2.4.1.31); cellodextrin phosphorylase (EC 2.4.1.49); chitobiose phosphorylase (EC 2.4.1.-); cyclic β-1,2-glucan synthase (EC 2.4.1.-); cellobionic acid phosphorylase (EC 2.4.1.321); β-1,2-oligoglucan phosphorylase (EC 2.4.1.-)
Mechanism Inverting
3D Structure Status( α / α ) 6
Catalytic Nucleophile/Basephosphate
Catalytic Proton DonorAsp
NoteFormerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L.
External resourcesCAZypedia;
Statistics GenBank accession (1859); Uniprot accession (337); PDB accession (28); 3D entries (7); cryst (0)
Bacteria
Protein Name EC#OrganismGenBankUniprot
PDB/3D Carbohydrate Ligands Resolution (Å)
 cellobiose phosphorylase (Cbp;CgCBP) 2.4.1.20 Cellulomonas gilvus ATCC13127 BAA28631.1 O66264
2CQS[A,B] β-D-Glcp
2.00
2CQT[A,B] β-D-Glcp
2.10
3ACS[A,B] β-D-Glcp
2.51
3ACT[A,B] β-D-Glcp
1.85
3AFJ[A,B] β-D-Glcp
1.90
3QFY[A,B] β-D-Glcp
<carb_like_IFM>
2.30
3QFZ[A,B] β-D-Glcp
<carb_like_NOJ>
2.39
3QG0[A,B] β-D-Glcp
<carb_like_NOJ>
2.70
 cellobiose phosphorylase (CbP) 2.4.1.20 Cellulomonas uda DSM 20108 AAQ20920.1 Q7WTR6
3RRS[A,B] 1.70
3RSY[A,B] 1.81
3S4A[A,B] β-D-Glcp-(1-4)-β-D-Glcp
1.99
3S4B[A,B] β-D-Glcp
2.40
3S4C[A] 2.40
3S4D[A] β-D-Glcp-(1-4)-β-D-Glcp
3.30
 β-1,2-oligoglucan phosphorylase (LpSOGP;Cphy_0694) 2.4.1.- Lachnoclostridium phytofermentans ISDg ABX41081.1 A9KJS6
5H3Z[A,B] 2.00
5H40[A,B] β-D-Glcp-(1-2)-β-D-Glcp
2.20
5H41[A,B] β-D-Glcp-(1-2)-α-D-Glcp
<carb_like_IFM>
2.00
5H42[A,B] α-D-Glcp
2.10
 cellodextrin-phosphorylase (Cdp;CtCDP;Cthe_2989) 2.4.1.49 Ruminiclostridium thermocellum ATCC 27405 ABN54185.1
BAA22081.1
A3DJQ6
O24780
5NZ7[A,B] 2.30
5NZ8[A,B] β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp-(1-4)-β-D-Glcp
3.00
 cellobiose phosphorylase (Cbp) 2.4.1.20 Ruminiclostridium thermocellum YM4 AAL67138.1 Q8VP44
3QDE[A,B] 2.40
 cellobionic acid phosphorylase 94B (Cep94B;CBAP;SdCBAP;Sde_0906) (Cep94B) 2.4.1.321 Saccharophagus degradans 2-40 ABD80168.1 Q21MB1
4ZLE[A] 2.10
4ZLF[A] β-D-Glcp-(1-1)-<non_carb>
1.60
4ZLG[A] β-D-Glcp
1.75
4ZLI[A] β-D-Glcp-(1-3)-α-D-GlcpA
1.80
 chitobiose phosphorylase (ChbP;VpChBP) 2.4.1.- Vibrio proteolyticus BAC87867.1 Q76IQ9
1V7V[A] 1.80
1V7W[A] α-D-GlcpNAc
β-D-GlcpNAc
1.60
1V7X[A] α-D-GlcpNAc
β-D-GlcpNAc
2.00
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