Glycoside Hydrolase Family 94

Known Activitiescellobiose phosphorylase (EC 2.4.1.20); cellodextrin phosphorylase (EC 2.4.1.49); chitobiose phosphorylase (EC 2.4.1.-); cyclic β-1,2-glucan synthase (EC 2.4.1.-)
Mechanism Inverting
3D Structure Status( α / α ) 6
Catalytic Nucleophile/Basephosphate
Catalytic Proton DonorAsp
NoteFormerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L.
External resourcesCAZypedia;
Statistics GenBank accession (530); Uniprot accession (325); PDB accession (12); 3D entries (4); cryst (1)
All (497) Archaea (5) Bacteria (484) Eukaryota (6) unclassified (2) Structure (4 - 1 cryst) Characterized (19)
Bacteria
Protein Name EC#OrganismGenBankUniprotPDB/3D
 cellobiose phosphorylase (CbP) 2.4.1.20 Cellulomonas uda DSM 20108 AAQ20920.1 Q7WTR6 cryst
 cellobiose phosphorylase (Cbp) 2.4.1.20 Clostridium thermocellum YM4 AAL67138.1 Q8VP44 3QDE[A,B]
 chitobiose phosphorylase (ChbP) 2.4.1.- Vibrio proteolyticus BAC87867.1 Q76IQ9 1V7V[A]
1V7W[A]
1V7X[A]
 cellobiose phosphorylase 2.4.1.20 [Cellvibrio] gilvus ATCC13127 BAA28631.1 O66264 2CQS[A,B]
2CQT[A,B]
3ACS[A,B]
3ACT[A,B]
3AFJ[A,B]
3QFY[A,B]
3QFZ[A,B]
3QG0[A,B]




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